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The map label for this gene is pys2 [H]

Identifier: 66047877

GI number: 66047877

Start: 5513236

End: 5515167

Strand: Direct

Name: pys2 [H]

Synonym: Psyr_4651

Alternate gene names: 66047877

Gene position: 5513236-5515167 (Clockwise)

Preceding gene: 66047873

Following gene: 66047878

Centisome position: 90.47

GC content: 50.72

Gene sequence:

>1932_bases
ATGTCACAGAACGAGATTACACTGCCCCCTATCATCGTTACACCTGATCCACCACTGCCTCAGCCTCCAGGTCCTATTCC
AGGAGGTGGTGTGATTGCCAAGCCGCTGCCATGGGACGGTAAAGTGCCTGCAGACGCTGAAATTGATAAGTTCTTTAGTC
AGCAAGGAATTAAAGGTGAGCAGTACACGATTTCTGTGGTGGCAACGGTAGCCACTACTCAACGAAACATTGAGCAAGCC
TTTACGGCCTATTTGCCGCAATTACCGGCAGACATTGACGCAGAAATTGCTGCTGCAGTCGGGCCCAATCCTTTATCCGC
ACTTGAAAAGGCCAAAACTGAAAAAAGTGTGGTGGATAACCTGATCACGCAGAACACTGCAGAACTGGCAAATGCCAACG
CAGCAGCCAGCGCTTTTTTTGGCAGAAATGTGTTGGCAGTAGAAATTAAAAAAAGCGCAGTAGATTTTGTAAATATCTTT
CAATCAAGACAAGATCGTGGCACCCCGCTCGAGGTTTTCAAAAGTTGGGAAGCGTCAGCTACCGCCGCCTATGCTGCGAA
AATTATTGAGGAGAAGATCCGAATACTCACGGAAAAGTCAGCTGCACTTTTACAGACCGTGGCTACGGCGCAGGCTGAAG
AGGATGCTCGCATTGCTGCAGAGGCAGAAGCTAAACGTTTGGCTGATGAAGCAGCGGCTGCAGAAGCCAAACGCTTGGCT
GATGAGGCAGCGGCTGCAGAAGCCAAACGCTTGGCTGATGAAGCAGCAGCTGCGGAAGCCAAACGCATTGCCGAAGAGCA
GGCTCGTATTGCTGCAGAGGCCGTTCGGACAGCTAATACATTCCGTGCGCCTGGCCCGCTTTCAGCTACCGCTCCCGTTA
TCATGACGGCTGCGGGAACGATTGCCGTTATCGAAGCAGCGACAGTTACCTTGCAAGCAGCTATCCGCTCCGCTGTTGCC
GCGCTAACTAATTTAGCCGCTGGTACGGCTTCGGGGCTTTTGGTCGGCGTGTCAGCTTTGGTCTATTCACCCAAACTGGC
TAACGGAGAGTTACCTGAGCGTTATGCTTTCAACACACCATTGTCGGATCTGACGCCTGAGCTTGGTAAAGACTTGCCAG
CGATTGCGGCATCTGGTGGTACTGTTGATTTGCCATTTCGACTTAGCTCTAAAACAGCAGCAGATGGGCAGTCTGAAGTT
TTTGTTGTCAAGACCGATGCTTTGACAGCATCCTCGAAAGTAAGAGTGGTATCAGCTGTTCTTGATGTAGAGCAGAACAC
CTATAGCGTGACAACGGGAGATGTACCACCCAGGATATTGACTTGGACACCGATTGTCAGTCCTGGGAATAGCTCAACAA
CATCGCCTGCTGAACAGCCAGCTCCACCCGTGTATACGGGGGCTGCAGTAACGCCCGTTGAAGGGAGGATAGATGCATTC
CCGGCTGTTTCAGAAGCCAGTTTTGATGATTTCATTACGGTTTTTCCGGCAGACTCTGGTTTACCACCCATCTATACGAT
GTTCCGGGATCGGCGAGAAGATCCGGGCGTTGCAACAGGTGTCGGTCAACCTGTATCGGGGATCTGGCTAGGGGCTGCAT
CGCAAGGTGAAGGGGCTCCCATTCCATCGCAGATTGCTGATCAGCTTCGCGGCAAGGAGTTCAAGAACTTCAGAGACTTT
CGGAAAGCTTTCTGGCTCGCTGTCGGTGCTGATCTAGAGTTGTCAAAACAATTCAAAGGTTCAAATAATACTCTGATTAA
AGGAGGCACTGCGCCATTTGCAATTCCGAGCGAGCAAGTTGGAGGGCGTGGTCAATTTGAGATTCATCACGTCATACCTG
TTCATCCAGCCGGAGCGGTTTACGATGTGGAAAACATGCGTATAATGACCCCCAAACTACATATTCAAACTCACACCAAG
AAAGAGGGATAG

Upstream 100 bases:

>100_bases
GTCATTTTTACTGTATATATATACAGCTATCTCATGCCTCGATACTATCCGTGCTGAGAGTTAGATTTAGCAAGGTGACC
CACAAATCAAGGAGAAAGAC

Downstream 100 bases:

>100_bases
TGAAATGGAATTTAAAAATAAATATGAAGAGTATACAGAGGCTGAATTTTTAGCGTTTCTTGAAGAGTTTTTTGTCAACA
AACAAAATCTAGAGGGTGAT

Product: S-type Pyocin

Products: NA

Alternate protein names: Killer protein [H]

Number of amino acids: Translated: 643; Mature: 642

Protein sequence:

>643_residues
MSQNEITLPPIIVTPDPPLPQPPGPIPGGGVIAKPLPWDGKVPADAEIDKFFSQQGIKGEQYTISVVATVATTQRNIEQA
FTAYLPQLPADIDAEIAAAVGPNPLSALEKAKTEKSVVDNLITQNTAELANANAAASAFFGRNVLAVEIKKSAVDFVNIF
QSRQDRGTPLEVFKSWEASATAAYAAKIIEEKIRILTEKSAALLQTVATAQAEEDARIAAEAEAKRLADEAAAAEAKRLA
DEAAAAEAKRLADEAAAAEAKRIAEEQARIAAEAVRTANTFRAPGPLSATAPVIMTAAGTIAVIEAATVTLQAAIRSAVA
ALTNLAAGTASGLLVGVSALVYSPKLANGELPERYAFNTPLSDLTPELGKDLPAIAASGGTVDLPFRLSSKTAADGQSEV
FVVKTDALTASSKVRVVSAVLDVEQNTYSVTTGDVPPRILTWTPIVSPGNSSTTSPAEQPAPPVYTGAAVTPVEGRIDAF
PAVSEASFDDFITVFPADSGLPPIYTMFRDRREDPGVATGVGQPVSGIWLGAASQGEGAPIPSQIADQLRGKEFKNFRDF
RKAFWLAVGADLELSKQFKGSNNTLIKGGTAPFAIPSEQVGGRGQFEIHHVIPVHPAGAVYDVENMRIMTPKLHIQTHTK
KEG

Sequences:

>Translated_643_residues
MSQNEITLPPIIVTPDPPLPQPPGPIPGGGVIAKPLPWDGKVPADAEIDKFFSQQGIKGEQYTISVVATVATTQRNIEQA
FTAYLPQLPADIDAEIAAAVGPNPLSALEKAKTEKSVVDNLITQNTAELANANAAASAFFGRNVLAVEIKKSAVDFVNIF
QSRQDRGTPLEVFKSWEASATAAYAAKIIEEKIRILTEKSAALLQTVATAQAEEDARIAAEAEAKRLADEAAAAEAKRLA
DEAAAAEAKRLADEAAAAEAKRIAEEQARIAAEAVRTANTFRAPGPLSATAPVIMTAAGTIAVIEAATVTLQAAIRSAVA
ALTNLAAGTASGLLVGVSALVYSPKLANGELPERYAFNTPLSDLTPELGKDLPAIAASGGTVDLPFRLSSKTAADGQSEV
FVVKTDALTASSKVRVVSAVLDVEQNTYSVTTGDVPPRILTWTPIVSPGNSSTTSPAEQPAPPVYTGAAVTPVEGRIDAF
PAVSEASFDDFITVFPADSGLPPIYTMFRDRREDPGVATGVGQPVSGIWLGAASQGEGAPIPSQIADQLRGKEFKNFRDF
RKAFWLAVGADLELSKQFKGSNNTLIKGGTAPFAIPSEQVGGRGQFEIHHVIPVHPAGAVYDVENMRIMTPKLHIQTHTK
KEG
>Mature_642_residues
SQNEITLPPIIVTPDPPLPQPPGPIPGGGVIAKPLPWDGKVPADAEIDKFFSQQGIKGEQYTISVVATVATTQRNIEQAF
TAYLPQLPADIDAEIAAAVGPNPLSALEKAKTEKSVVDNLITQNTAELANANAAASAFFGRNVLAVEIKKSAVDFVNIFQ
SRQDRGTPLEVFKSWEASATAAYAAKIIEEKIRILTEKSAALLQTVATAQAEEDARIAAEAEAKRLADEAAAAEAKRLAD
EAAAAEAKRLADEAAAAEAKRIAEEQARIAAEAVRTANTFRAPGPLSATAPVIMTAAGTIAVIEAATVTLQAAIRSAVAA
LTNLAAGTASGLLVGVSALVYSPKLANGELPERYAFNTPLSDLTPELGKDLPAIAASGGTVDLPFRLSSKTAADGQSEVF
VVKTDALTASSKVRVVSAVLDVEQNTYSVTTGDVPPRILTWTPIVSPGNSSTTSPAEQPAPPVYTGAAVTPVEGRIDAFP
AVSEASFDDFITVFPADSGLPPIYTMFRDRREDPGVATGVGQPVSGIWLGAASQGEGAPIPSQIADQLRGKEFKNFRDFR
KAFWLAVGADLELSKQFKGSNNTLIKGGTAPFAIPSEQVGGRGQFEIHHVIPVHPAGAVYDVENMRIMTPKLHIQTHTKK
EG

Specific function: Causes breakdown of chromosomal DNA as well as complete inhibition of lipid synthesis in sensitive cells [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the colicin/pyosin nuclease family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003615
- InterPro:   IPR003060
- InterPro:   IPR010715
- InterPro:   IPR016128 [H]

Pfam domain/function: PF06958 Pyocin_S [H]

EC number: 3.1.21.1

Molecular weight: Translated: 67400; Mature: 67268

Theoretical pI: Translated: 4.78; Mature: 4.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
0.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
0.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQNEITLPPIIVTPDPPLPQPPGPIPGGGVIAKPLPWDGKVPADAEIDKFFSQQGIKGE
CCCCCCCCCCEEECCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCHHHHHHHHCCCCCC
QYTISVVATVATTQRNIEQAFTAYLPQLPADIDAEIAAAVGPNPLSALEKAKTEKSVVDN
CEEEEEEEEHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHH
LITQNTAELANANAAASAFFGRNVLAVEIKKSAVDFVNIFQSRQDRGTPLEVFKSWEASA
HHHCCHHHHHCCCHHHHHHHCCCEEEEEEHHHHHHHHHHHHHHCCCCCHHHHHHCCCCHH
TAAYAAKIIEEKIRILTEKSAALLQTVATAQAEEDARIAAEAEAKRLADEAAAAEAKRLA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
DEAAAAEAKRLADEAAAAEAKRIAEEQARIAAEAVRTANTFRAPGPLSATAPVIMTAAGT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEECCCC
IAVIEAATVTLQAAIRSAVAALTNLAAGTASGLLVGVSALVYSPKLANGELPERYAFNTP
EEEHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCC
LSDLTPELGKDLPAIAASGGTVDLPFRLSSKTAADGQSEVFVVKTDALTASSKVRVVSAV
HHHCCHHHCCCCCCEECCCCEEEEEEEECCCCCCCCCCCEEEEEECCCCCCCHHHHHHHH
LDVEQNTYSVTTGDVPPRILTWTPIVSPGNSSTTSPAEQPAPPVYTGAAVTPVEGRIDAF
HHCCCCEEEEECCCCCCCEEEEECEECCCCCCCCCCCCCCCCCCCCCCEECCCCCCCCCC
PAVSEASFDDFITVFPADSGLPPIYTMFRDRREDPGVATGVGQPVSGIWLGAASQGEGAP
CCCCCCCCCCEEEEEECCCCCCHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCCCCCCC
IPSQIADQLRGKEFKNFRDFRKAFWLAVGADLELSKQFKGSNNTLIKGGTAPFAIPSEQV
CHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCEEHHHCCCCCCCEEECCCCCCCCCHHHC
GGRGQFEIHHVIPVHPAGAVYDVENMRIMTPKLHIQTHTKKEG
CCCCCEEEEEEEEECCCCCEEECCCCEEECCEEEEEECCCCCC
>Mature Secondary Structure 
SQNEITLPPIIVTPDPPLPQPPGPIPGGGVIAKPLPWDGKVPADAEIDKFFSQQGIKGE
CCCCCCCCCEEECCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCHHHHHHHHCCCCCC
QYTISVVATVATTQRNIEQAFTAYLPQLPADIDAEIAAAVGPNPLSALEKAKTEKSVVDN
CEEEEEEEEHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHH
LITQNTAELANANAAASAFFGRNVLAVEIKKSAVDFVNIFQSRQDRGTPLEVFKSWEASA
HHHCCHHHHHCCCHHHHHHHCCCEEEEEEHHHHHHHHHHHHHHCCCCCHHHHHHCCCCHH
TAAYAAKIIEEKIRILTEKSAALLQTVATAQAEEDARIAAEAEAKRLADEAAAAEAKRLA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
DEAAAAEAKRLADEAAAAEAKRIAEEQARIAAEAVRTANTFRAPGPLSATAPVIMTAAGT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEECCCC
IAVIEAATVTLQAAIRSAVAALTNLAAGTASGLLVGVSALVYSPKLANGELPERYAFNTP
EEEHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCC
LSDLTPELGKDLPAIAASGGTVDLPFRLSSKTAADGQSEVFVVKTDALTASSKVRVVSAV
HHHCCHHHCCCCCCEECCCCEEEEEEEECCCCCCCCCCCEEEEEECCCCCCCHHHHHHHH
LDVEQNTYSVTTGDVPPRILTWTPIVSPGNSSTTSPAEQPAPPVYTGAAVTPVEGRIDAF
HHCCCCEEEEECCCCCCCEEEEECEECCCCCCCCCCCCCCCCCCCCCCEECCCCCCCCCC
PAVSEASFDDFITVFPADSGLPPIYTMFRDRREDPGVATGVGQPVSGIWLGAASQGEGAP
CCCCCCCCCCEEEEEECCCCCCHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCCCCCCC
IPSQIADQLRGKEFKNFRDFRKAFWLAVGADLELSKQFKGSNNTLIKGGTAPFAIPSEQV
CHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCEEHHHCCCCCCCEEECCCCCCCCCHHHC
GGRGQFEIHHVIPVHPAGAVYDVENMRIMTPKLHIQTHTKKEG
CCCCCEEEEEEEEECCCCCEEECCCCEEECCEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8491711; 10984043 [H]