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The map label for this gene is agp [H]

Identifier: 66047727

GI number: 66047727

Start: 5363481

End: 5364764

Strand: Direct

Name: agp [H]

Synonym: Psyr_4500

Alternate gene names: 66047727

Gene position: 5363481-5364764 (Clockwise)

Preceding gene: 66047726

Following gene: 66047729

Centisome position: 88.02

GC content: 62.93

Gene sequence:

>1284_bases
ATGACTTCTAGGCCCGTGATGATGAAATCGACTTTGTTGAAGTATGCGGGGTTGGCGCTGGGCGTGTGCCTGGCGCTTCC
ATCGGTGGCCGCGCCTCTGGACACCACCGGCCTGCGCCTGGACAAGGTGGTACTGGTGATGCGCCACGGTATCCGCCCGG
CTACCGACACCGCCAAGTTGCAACGCTGGTCGGCCAGGACCTGGCCAGCGTTTGGTGCCCGCGACGGTCAGTTGACCGAG
CATGGCCGCGCTGCCACCGTGCTGCTGGGCCAATGGCAACGCCACACGCTGGATTCGCTCGGGCTGTTCAAGACGGGGCA
GTGCCCGCAGGCGGGGGATGCCTACGTGTGGTCCAGCCCGGTAGCGCGAACCCAGGCCACCGGTGCGGCGCTGGTAAAGG
GGATGTTCCCGGGCTGCAACATAGCAGTACACCATGGGCGTGAGAGTGAAGACCGGCTGTTCCACGGCGGCGAAAATGGC
CTGGCACCGCTGGACCCGGCGCGCACCCAGGCGGCGATGCTGGCAGCCATGGGTGGCAGCGTCGACGCAGCTCGCGAGCG
CTTTGCCGCGCCGTTGCTGGCCATGCAGCAATTGGCAGGCGTGCCGACGAACTGCGGGAAAAAGACCTGTGCCTTGAGCA
AGCAACCCTGGGCGCTGAAAGAGAAGGCAGGCGTCACCAAGCTCAGCGGACCGTTGAGCGTGGGCGCGGCCATGAGCGAA
ACCTTCCGCATGCAATACGCCGAAGGCTTGCCACTGGACCAGGTGGCGTTTGGCGAAGGGCGCACCGCTGCAGACGTATC
GCGCTTGATGGCCTTGCGTTCGGGCAAATACGCGCTGAGCAATCACGTACCTTACATCGCCCAGCGGGGTGCTTCGCAAT
TGCTCGGACAAATCCTCCTGGCTCTGCAACCGACATCCGTGGGCAGCCCGCCGGGTAGCAAATGGTTGGCGTACGTAGGC
CACGACAGCAACATCGCGCAGTTGCGAACGCTGCTGGGTTTTGACTGGAAAATCGCCGAATACCCCGAGAATGACGCAGC
ACCAGGCGGCACCATCGTGTTTGAACGCTGGGCGAACGACCGCACGGGCGCGCAGTTTGTCAGCGTTGCCTATGTGGCGC
AGAGCCTGGACCAGTTACGCAATTTGTCCGGTGAACCGCCGTATCAGGTGCAGTACCCGGGGTATGCAGGAGAGCAATTG
ATGCCATTGAACGGGTTTGTTGCCGATATGGAAAAGCGCATCGATCGGTCTGCGACTGAGGTACAGCACTACGACAACCC
TTGA

Upstream 100 bases:

>100_bases
GCCAACCTCACCAACCTGGCCAACAAAAAGTACTGGACCTACTATCAGGAAAACTACCTGCAACCGGGTTCGCCACGTAC
GTTAAGCCTCAATACCCGCT

Downstream 100 bases:

>100_bases
AACACCAGCTTTGGCCCAACAACAGTCAACAGCACCACCCAAAAACCGCCAATACCTGTCCACCCAGCGCCCAACGAATC
AAACAAAAAACCGCTCCTTC

Product: 4-phytase

Products: NA

Alternate protein names: G1Pase [H]

Number of amino acids: Translated: 427; Mature: 426

Protein sequence:

>427_residues
MTSRPVMMKSTLLKYAGLALGVCLALPSVAAPLDTTGLRLDKVVLVMRHGIRPATDTAKLQRWSARTWPAFGARDGQLTE
HGRAATVLLGQWQRHTLDSLGLFKTGQCPQAGDAYVWSSPVARTQATGAALVKGMFPGCNIAVHHGRESEDRLFHGGENG
LAPLDPARTQAAMLAAMGGSVDAARERFAAPLLAMQQLAGVPTNCGKKTCALSKQPWALKEKAGVTKLSGPLSVGAAMSE
TFRMQYAEGLPLDQVAFGEGRTAADVSRLMALRSGKYALSNHVPYIAQRGASQLLGQILLALQPTSVGSPPGSKWLAYVG
HDSNIAQLRTLLGFDWKIAEYPENDAAPGGTIVFERWANDRTGAQFVSVAYVAQSLDQLRNLSGEPPYQVQYPGYAGEQL
MPLNGFVADMEKRIDRSATEVQHYDNP

Sequences:

>Translated_427_residues
MTSRPVMMKSTLLKYAGLALGVCLALPSVAAPLDTTGLRLDKVVLVMRHGIRPATDTAKLQRWSARTWPAFGARDGQLTE
HGRAATVLLGQWQRHTLDSLGLFKTGQCPQAGDAYVWSSPVARTQATGAALVKGMFPGCNIAVHHGRESEDRLFHGGENG
LAPLDPARTQAAMLAAMGGSVDAARERFAAPLLAMQQLAGVPTNCGKKTCALSKQPWALKEKAGVTKLSGPLSVGAAMSE
TFRMQYAEGLPLDQVAFGEGRTAADVSRLMALRSGKYALSNHVPYIAQRGASQLLGQILLALQPTSVGSPPGSKWLAYVG
HDSNIAQLRTLLGFDWKIAEYPENDAAPGGTIVFERWANDRTGAQFVSVAYVAQSLDQLRNLSGEPPYQVQYPGYAGEQL
MPLNGFVADMEKRIDRSATEVQHYDNP
>Mature_426_residues
TSRPVMMKSTLLKYAGLALGVCLALPSVAAPLDTTGLRLDKVVLVMRHGIRPATDTAKLQRWSARTWPAFGARDGQLTEH
GRAATVLLGQWQRHTLDSLGLFKTGQCPQAGDAYVWSSPVARTQATGAALVKGMFPGCNIAVHHGRESEDRLFHGGENGL
APLDPARTQAAMLAAMGGSVDAARERFAAPLLAMQQLAGVPTNCGKKTCALSKQPWALKEKAGVTKLSGPLSVGAAMSET
FRMQYAEGLPLDQVAFGEGRTAADVSRLMALRSGKYALSNHVPYIAQRGASQLLGQILLALQPTSVGSPPGSKWLAYVGH
DSNIAQLRTLLGFDWKIAEYPENDAAPGGTIVFERWANDRTGAQFVSVAYVAQSLDQLRNLSGEPPYQVQYPGYAGEQLM
PLNGFVADMEKRIDRSATEVQHYDNP

Specific function: Absolutely required for the growth of E.coli in a high- phosphate medium containing G-1-P as the sole carbon source [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the histidine acid phosphatase family [H]

Homologues:

Organism=Escherichia coli, GI1787237, Length=384, Percent_Identity=31.7708333333333, Blast_Score=163, Evalue=2e-41,
Organism=Escherichia coli, GI1787215, Length=404, Percent_Identity=29.7029702970297, Blast_Score=162, Evalue=5e-41,

Paralogues:

None

Copy number: 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 380 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000560 [H]

Pfam domain/function: PF00328 Acid_phosphat_A [H]

EC number: =3.1.3.10 [H]

Molecular weight: Translated: 45839; Mature: 45708

Theoretical pI: Translated: 9.25; Mature: 9.25

Prosite motif: PS00616 HIS_ACID_PHOSPHAT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSRPVMMKSTLLKYAGLALGVCLALPSVAAPLDTTGLRLDKVVLVMRHGIRPATDTAKL
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCCCHHHH
QRWSARTWPAFGARDGQLTEHGRAATVLLGQWQRHTLDSLGLFKTGQCPQAGDAYVWSSP
HHHCCCCCCCCCCCCCCCCCCCCEEEEEECCHHHHHHHHCCCCCCCCCCCCCCCEEECCC
VARTQATGAALVKGMFPGCNIAVHHGRESEDRLFHGGENGLAPLDPARTQAAMLAAMGGS
CHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCEEECCCCCCCCCCHHHHHHHHHHHHCCC
VDAARERFAAPLLAMQQLAGVPTNCGKKTCALSKQPWALKEKAGVTKLSGPLSVGAAMSE
HHHHHHHHHHHHHHHHHHCCCCCCCCCCHHCCCCCCCCHHHHCCCEECCCCHHHHHHHHH
TFRMQYAEGLPLDQVAFGEGRTAADVSRLMALRSGKYALSNHVPYIAQRGASQLLGQILL
HHHHHHHCCCCCCCEECCCCCCHHHHHHHHHHHCCCCHHHCCCCHHHHCCHHHHHHHHHH
ALQPTSVGSPPGSKWLAYVGHDSNIAQLRTLLGFDWKIAEYPENDAAPGGTIVFERWAND
HCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHCCCCEEECCCCCCCCCCCEEEEEECCCC
RTGAQFVSVAYVAQSLDQLRNLSGEPPYQVQYPGYAGEQLMPLNGFVADMEKRIDRSATE
CCHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCEECCCHHHHHHHHHHHHHHHH
VQHYDNP
HHCCCCC
>Mature Secondary Structure 
TSRPVMMKSTLLKYAGLALGVCLALPSVAAPLDTTGLRLDKVVLVMRHGIRPATDTAKL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCCCHHHH
QRWSARTWPAFGARDGQLTEHGRAATVLLGQWQRHTLDSLGLFKTGQCPQAGDAYVWSSP
HHHCCCCCCCCCCCCCCCCCCCCEEEEEECCHHHHHHHHCCCCCCCCCCCCCCCEEECCC
VARTQATGAALVKGMFPGCNIAVHHGRESEDRLFHGGENGLAPLDPARTQAAMLAAMGGS
CHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCEEECCCCCCCCCCHHHHHHHHHHHHCCC
VDAARERFAAPLLAMQQLAGVPTNCGKKTCALSKQPWALKEKAGVTKLSGPLSVGAAMSE
HHHHHHHHHHHHHHHHHHCCCCCCCCCCHHCCCCCCCCHHHHCCCEECCCCHHHHHHHHH
TFRMQYAEGLPLDQVAFGEGRTAADVSRLMALRSGKYALSNHVPYIAQRGASQLLGQILL
HHHHHHHCCCCCCCEECCCCCCHHHHHHHHHHHCCCCHHHCCCCHHHHCCHHHHHHHHHH
ALQPTSVGSPPGSKWLAYVGHDSNIAQLRTLLGFDWKIAEYPENDAAPGGTIVFERWAND
HCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHCCCCEEECCCCCCCCCCCEEEEEECCCC
RTGAQFVSVAYVAQSLDQLRNLSGEPPYQVQYPGYAGEQLMPLNGFVADMEKRIDRSATE
CCHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCEECCCHHHHHHHHHHHHHHHH
VQHYDNP
HHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2153660; 8905232; 9278503; 9298646; 12782623 [H]