Click here to switch to the map view.

The map label for this gene is braD [H]

Identifier: 66047654

GI number: 66047654

Start: 5295137

End: 5296726

Strand: Direct

Name: braD [H]

Synonym: Psyr_4427

Alternate gene names: 66047654

Gene position: 5295137-5296726 (Clockwise)

Preceding gene: 66047653

Following gene: 66047655

Centisome position: 86.9

GC content: 62.52

Gene sequence:

>1590_bases
ATGCCTTCTTATTTTTACCGTTTGATCCTCGCCATCTGCCTGCTGCTGCCTTTGGCTGCCCAGGCCGGTGATGCCAGCGA
CTTTGTCGCAGCCAGTTCTTCGCAACAGGCAGAACTGCTGGAGGCCTGGGCCGCCAAGCCTGTGCCGGAACGCGTCGAGC
TGCTTGAAGCCTTACGCGATGGCCGTGTGGGTGCCGACAGCAGCAAGCGCGCCTGGATCGAAAACAACGATCAATACGTG
GCCGTCGATGCACAGGCACCAGCCGCCGCCGATGCGCCAACGCCTGACGAACCGCGTAAATTGCGCTTGAACAACCGGCT
GCGCGGCCTGGTTGAAACCGCGCTGGCCAGCCACCAGTTGCTGGCAGCCGATACACGACTGCGCATGGCCGCCGCCCTGC
AATTGCAAAAAAGCGCCCGCCCTGCACAACTGGAGTTGCTGACCGAACGCGTGGCCGGCGAAACAGATTCCAGCGTCAAA
AGCGCACTGACCCTGGCGCTGGCCAACCTGCAAATGGTCGATTCCAGCCCGACAGTACGTCTGGCTGCCGTGCGACTGCT
GGGCGAAACCGGTGATCCGCTGGCGCGTACCCGTCTTGAATCATTGCTGGCGCCCGGCGTGGAAACCGACGCTTCGGTAC
GCGTGGCGGCTGAAACCAGCCTGGCGCAGGTCAAGCGCAAGCTGATGATCGGTGAGATCCTCGGCCAGGCGTTTAGCGGC
ATGTCGCTGGGCTCTATCCTGTTGCTGGCGGCGCTGGGTCTGGCGATCACCTTCGGCCTGCTGGGCGTGATCAACATGGC
CCATGGCGAGATGCTGATGCTGGGGGCCTACTCCACCTACGTCGTGCAACTGATGCTGCAGCGCTACGCACCGGGGGCCA
TCGAGTACTACCCGCTGATCGCGTTGCCGGTGGCGTTCTTTGTCACCGCCGCGATCGGCATGGCCCTGGAGCGTACGGTC
ATTCGTCATCTGTATGGCCGCCCACTGGAAACCCTGCTGGCGACCTGGGGCATCAGCCTGATGCTGATTCAGGCCGTGCG
TCTGGCCTTCGGCGCGCAGAACGTCGAAGTCGCCAACCCGCAATGGCTGTCTGGCGGCATCCAGGTGCTGCCTAATCTGG
TGCTGCCTTACAACCGCATCGTGATCATCGCGTTCGCGCTGTTCGTGGTGGTGCTGACCTGGCTGCTGCTGAACAAGACC
CGCCTGGGCCTGAACGTGCGCGCCGTCACCCAGAACCGCAACATGGCCGCCTGCTGTGGCGTACCGACCGGGCGCATCGA
CATGATGGCCTTCGGTCTGGGTTCAGGTATCGCAGGTCTGGGGGGTGTAGCCCTGAGCCAGATCGGCAACGTGGGCCCTG
ATCTTGGCCAAAGCTACATCATCGATTCGTTCCTGGTGGTCGTGCTCGGCGGTGTCGGGCAACTGGCCGGTAGCGTGATG
GCGGCCTTCGGGCTGGGCATCGCCAACAAGATTCTCGAACCGCAAATCGGCGCCGTGTTGGGCAAGATCCTGATCCTGGC
GCTGATCATTCTGTTTATCCAGAAACGTCCGCAAGGCCTCTTCGCATTGAAAGGACGGGTGATCGACTGA

Upstream 100 bases:

>100_bases
GCCAGAATCAAAAACAGCCCCCGCATGTGACGCTTGGCGAAGGGCGTGAAGGACCGTCTCAAGAGTAGACGACGCCATAC
TGAATCAAGGCCATATTGAT

Downstream 100 bases:

>100_bases
TGAGCCAGCCATTGATAGTAACTGCCGCGCAAAAAGTCGGGCCCAGAGGCACGCTGACCGCAGGCTGCGTAATCCTTGCA
GTGCTGCTGGCACTGCCGCT

Product: inner-membrane translocator

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 529; Mature: 528

Protein sequence:

>529_residues
MPSYFYRLILAICLLLPLAAQAGDASDFVAASSSQQAELLEAWAAKPVPERVELLEALRDGRVGADSSKRAWIENNDQYV
AVDAQAPAAADAPTPDEPRKLRLNNRLRGLVETALASHQLLAADTRLRMAAALQLQKSARPAQLELLTERVAGETDSSVK
SALTLALANLQMVDSSPTVRLAAVRLLGETGDPLARTRLESLLAPGVETDASVRVAAETSLAQVKRKLMIGEILGQAFSG
MSLGSILLLAALGLAITFGLLGVINMAHGEMLMLGAYSTYVVQLMLQRYAPGAIEYYPLIALPVAFFVTAAIGMALERTV
IRHLYGRPLETLLATWGISLMLIQAVRLAFGAQNVEVANPQWLSGGIQVLPNLVLPYNRIVIIAFALFVVVLTWLLLNKT
RLGLNVRAVTQNRNMAACCGVPTGRIDMMAFGLGSGIAGLGGVALSQIGNVGPDLGQSYIIDSFLVVVLGGVGQLAGSVM
AAFGLGIANKILEPQIGAVLGKILILALIILFIQKRPQGLFALKGRVID

Sequences:

>Translated_529_residues
MPSYFYRLILAICLLLPLAAQAGDASDFVAASSSQQAELLEAWAAKPVPERVELLEALRDGRVGADSSKRAWIENNDQYV
AVDAQAPAAADAPTPDEPRKLRLNNRLRGLVETALASHQLLAADTRLRMAAALQLQKSARPAQLELLTERVAGETDSSVK
SALTLALANLQMVDSSPTVRLAAVRLLGETGDPLARTRLESLLAPGVETDASVRVAAETSLAQVKRKLMIGEILGQAFSG
MSLGSILLLAALGLAITFGLLGVINMAHGEMLMLGAYSTYVVQLMLQRYAPGAIEYYPLIALPVAFFVTAAIGMALERTV
IRHLYGRPLETLLATWGISLMLIQAVRLAFGAQNVEVANPQWLSGGIQVLPNLVLPYNRIVIIAFALFVVVLTWLLLNKT
RLGLNVRAVTQNRNMAACCGVPTGRIDMMAFGLGSGIAGLGGVALSQIGNVGPDLGQSYIIDSFLVVVLGGVGQLAGSVM
AAFGLGIANKILEPQIGAVLGKILILALIILFIQKRPQGLFALKGRVID
>Mature_528_residues
PSYFYRLILAICLLLPLAAQAGDASDFVAASSSQQAELLEAWAAKPVPERVELLEALRDGRVGADSSKRAWIENNDQYVA
VDAQAPAAADAPTPDEPRKLRLNNRLRGLVETALASHQLLAADTRLRMAAALQLQKSARPAQLELLTERVAGETDSSVKS
ALTLALANLQMVDSSPTVRLAAVRLLGETGDPLARTRLESLLAPGVETDASVRVAAETSLAQVKRKLMIGEILGQAFSGM
SLGSILLLAALGLAITFGLLGVINMAHGEMLMLGAYSTYVVQLMLQRYAPGAIEYYPLIALPVAFFVTAAIGMALERTVI
RHLYGRPLETLLATWGISLMLIQAVRLAFGAQNVEVANPQWLSGGIQVLPNLVLPYNRIVIIAFALFVVVLTWLLLNKTR
LGLNVRAVTQNRNMAACCGVPTGRIDMMAFGLGSGIAGLGGVALSQIGNVGPDLGQSYIIDSFLVVVLGGVGQLAGSVMA
AFGLGIANKILEPQIGAVLGKILILALIILFIQKRPQGLFALKGRVID

Specific function: Component of the high affinity leucine, isoleucine, valine, transport system (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG0559

COG function: function code E; Branched-chain amino acid ABC-type transport system, permease components

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the binding-protein-dependent transport system permease family. LivHM subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789866, Length=296, Percent_Identity=28.7162162162162, Blast_Score=94, Evalue=3e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001851 [H]

Pfam domain/function: PF02653 BPD_transp_2 [H]

EC number: NA

Molecular weight: Translated: 56179; Mature: 56048

Theoretical pI: Translated: 9.00; Mature: 9.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSYFYRLILAICLLLPLAAQAGDASDFVAASSSQQAELLEAWAAKPVPERVELLEALRD
CCHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHHHHC
GRVGADSSKRAWIENNDQYVAVDAQAPAAADAPTPDEPRKLRLNNRLRGLVETALASHQL
CCCCCCCCCCCCEECCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
LAADTRLRMAAALQLQKSARPAQLELLTERVAGETDSSVKSALTLALANLQMVDSSPTVR
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHEECCCCCHH
LAAVRLLGETGDPLARTRLESLLAPGVETDASVRVAAETSLAQVKRKLMIGEILGQAFSG
HHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHCC
MSLGSILLLAALGLAITFGLLGVINMAHGEMLMLGAYSTYVVQLMLQRYAPGAIEYYPLI
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHCCCCCCHHHHHH
ALPVAFFVTAAIGMALERTVIRHLYGRPLETLLATWGISLMLIQAVRLAFGAQNVEVANP
HHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCC
QWLSGGIQVLPNLVLPYNRIVIIAFALFVVVLTWLLLNKTRLGLNVRAVTQNRNMAACCG
HHHCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCHHCCCEEEEEECCCCEEEECC
VPTGRIDMMAFGLGSGIAGLGGVALSQIGNVGPDLGQSYIIDSFLVVVLGGVGQLAGSVM
CCCCCHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHH
AAFGLGIANKILEPQIGAVLGKILILALIILFIQKRPQGLFALKGRVID
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCC
>Mature Secondary Structure 
PSYFYRLILAICLLLPLAAQAGDASDFVAASSSQQAELLEAWAAKPVPERVELLEALRD
CHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHHHHC
GRVGADSSKRAWIENNDQYVAVDAQAPAAADAPTPDEPRKLRLNNRLRGLVETALASHQL
CCCCCCCCCCCCEECCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
LAADTRLRMAAALQLQKSARPAQLELLTERVAGETDSSVKSALTLALANLQMVDSSPTVR
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHEECCCCCHH
LAAVRLLGETGDPLARTRLESLLAPGVETDASVRVAAETSLAQVKRKLMIGEILGQAFSG
HHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHCC
MSLGSILLLAALGLAITFGLLGVINMAHGEMLMLGAYSTYVVQLMLQRYAPGAIEYYPLI
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHCCCCCCHHHHHH
ALPVAFFVTAAIGMALERTVIRHLYGRPLETLLATWGISLMLIQAVRLAFGAQNVEVANP
HHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCC
QWLSGGIQVLPNLVLPYNRIVIIAFALFVVVLTWLLLNKTRLGLNVRAVTQNRNMAACCG
HHHCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCHHCCCEEEEEECCCCEEEECC
VPTGRIDMMAFGLGSGIAGLGGVALSQIGNVGPDLGQSYIIDSFLVVVLGGVGQLAGSVM
CCCCCHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHH
AAFGLGIANKILEPQIGAVLGKILILALIILFIQKRPQGLFALKGRVID
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2120183; 10984043; 2509433 [H]