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The map label for this gene is purD [H]

Identifier: 66047634

GI number: 66047634

Start: 5269495

End: 5270790

Strand: Direct

Name: purD [H]

Synonym: Psyr_4407

Alternate gene names: 66047634

Gene position: 5269495-5270790 (Clockwise)

Preceding gene: 66047633

Following gene: 66047635

Centisome position: 86.47

GC content: 61.34

Gene sequence:

>1296_bases
TTGAATATTTTGATCATTGGCAGCGGCGGTCGTGAACACGCCCTGGCATGGAAAGTCGCCCAGGACCCGCGCGTGCAGAA
GGTGTTTGTCGCACCGGGCAATGCGGGCACGGCGATCGAGGCCAAATGCGAGAACGTGGCCATCGACGTTCTGGCACTGG
AGCAACTGGCGGATTTCGCTGAAAAGAACGTTGCGCTGACCATCGTCGGCCCTGAAGTACCGCTGGTTGCAGGCGTCGTC
GACCTGTTCCGCAGCCGTGGCCTGGACTGTTTCGGCCCGACTGCCGGTGCTGCGCAGCTCGAAGGTTCCAAGGCGTTCAC
CAAGGACTTCCTGGCGCGCCACAAGATTCCTACTGCCGACTATCAGAACTTCACCGAGATCGAGCCTGCTCTGGCCTATC
TGCGTGAAAAGGGCGCGCCGATTGTCATCAAGGCCGACGGCCTGGCCGCAGGCAAGGGCGTGATCGTCGCCATGACCCTG
GCAGAAGCCGAAGACGCCGTACGTGACATGCTGGCTGGCAACGCCTTTGGCGACGCCGGTTCGCGCGTTGTCATCGAAGA
GTTTCTCGATGGCGAAGAAGCCAGTTTCATTGTCATGGTCGACGGCAAGAATGTATTGCCCATGGCCACCAGCCAGGACC
ACAAGCGCGTCGGCGACGGCGACAGCGGGCCGAACACCGGCGGCATGGGTGCCTACTCCCCTGCCCCGGTGGTCACCGCC
GAGGTCCATCAACGTGTCATGGACCTGGTTATCTGGCCGACCGTCAGGGGCATGGCCGATGAAGGCAACGTTTACACCGG
CTTCCTCTATGCTGGTCTGATGATCGATAAAGCGGGTAACCCGAAAGTCATCGAGTTCAATTGCCGCTTCGGCGACCCGG
AAACCCAGCCGGTCATGCTGCGTCTGCAATCGAGCCTGGTACTGCTGGTCGAAGCTGCACTGGCGCAGGCGCTGGACAAG
ATCGAAGCACAGTGGGACCCGCGCCCGAGTCTGGGTATCGTGCTGGCCGCCGGGGGTTATCCTGGCGACTACGCCAAAGG
CGCGGTCATCGAAGGTCTGGACGCAGCGGCACAGCTCGAAGGCAAGATCTTCCATGCAGGCACAGCCTTGCAGGATGAGC
GTGTCGTGACCAGCGGTGGTCGTGTACTGTGTGCCACAGCACTGGGTGACAGCGTGGGCAACGCGCAGGAAAATGCCTAC
GCGCTGGCCGCAAAAGTCGACTGGCAAGGGTGTTTCTACCGCAAGGACATTGGCTACCGAGCCATTGCCCGTGAGCGTGG
CGAAGACCAGGAATAA

Upstream 100 bases:

>100_bases
AACCATTCAACGCCACAAGCTAAACACTGCGCTTTTACTTGCAGCTTGCAGCTTGCAGCTTTTAGCTAACAGCTTGTAGC
TACTCCAAAGGAGTCTGTTT

Downstream 100 bases:

>100_bases
TCGATCGGACCAGCCTCTTCAAACAGAGGCTGGCCGGTTGCTCCGGTATTCGCGGTTTTTACCAGGCATTCACTCACGAA
GGGATTTCATTGTGCGCTGG

Product: phosphoribosylamine--glycine ligase

Products: NA

Alternate protein names: GARS; Glycinamide ribonucleotide synthetase; Phosphoribosylglycinamide synthetase [H]

Number of amino acids: Translated: 431; Mature: 431

Protein sequence:

>431_residues
MNILIIGSGGREHALAWKVAQDPRVQKVFVAPGNAGTAIEAKCENVAIDVLALEQLADFAEKNVALTIVGPEVPLVAGVV
DLFRSRGLDCFGPTAGAAQLEGSKAFTKDFLARHKIPTADYQNFTEIEPALAYLREKGAPIVIKADGLAAGKGVIVAMTL
AEAEDAVRDMLAGNAFGDAGSRVVIEEFLDGEEASFIVMVDGKNVLPMATSQDHKRVGDGDSGPNTGGMGAYSPAPVVTA
EVHQRVMDLVIWPTVRGMADEGNVYTGFLYAGLMIDKAGNPKVIEFNCRFGDPETQPVMLRLQSSLVLLVEAALAQALDK
IEAQWDPRPSLGIVLAAGGYPGDYAKGAVIEGLDAAAQLEGKIFHAGTALQDERVVTSGGRVLCATALGDSVGNAQENAY
ALAAKVDWQGCFYRKDIGYRAIARERGEDQE

Sequences:

>Translated_431_residues
MNILIIGSGGREHALAWKVAQDPRVQKVFVAPGNAGTAIEAKCENVAIDVLALEQLADFAEKNVALTIVGPEVPLVAGVV
DLFRSRGLDCFGPTAGAAQLEGSKAFTKDFLARHKIPTADYQNFTEIEPALAYLREKGAPIVIKADGLAAGKGVIVAMTL
AEAEDAVRDMLAGNAFGDAGSRVVIEEFLDGEEASFIVMVDGKNVLPMATSQDHKRVGDGDSGPNTGGMGAYSPAPVVTA
EVHQRVMDLVIWPTVRGMADEGNVYTGFLYAGLMIDKAGNPKVIEFNCRFGDPETQPVMLRLQSSLVLLVEAALAQALDK
IEAQWDPRPSLGIVLAAGGYPGDYAKGAVIEGLDAAAQLEGKIFHAGTALQDERVVTSGGRVLCATALGDSVGNAQENAY
ALAAKVDWQGCFYRKDIGYRAIARERGEDQE
>Mature_431_residues
MNILIIGSGGREHALAWKVAQDPRVQKVFVAPGNAGTAIEAKCENVAIDVLALEQLADFAEKNVALTIVGPEVPLVAGVV
DLFRSRGLDCFGPTAGAAQLEGSKAFTKDFLARHKIPTADYQNFTEIEPALAYLREKGAPIVIKADGLAAGKGVIVAMTL
AEAEDAVRDMLAGNAFGDAGSRVVIEEFLDGEEASFIVMVDGKNVLPMATSQDHKRVGDGDSGPNTGGMGAYSPAPVVTA
EVHQRVMDLVIWPTVRGMADEGNVYTGFLYAGLMIDKAGNPKVIEFNCRFGDPETQPVMLRLQSSLVLLVEAALAQALDK
IEAQWDPRPSLGIVLAAGGYPGDYAKGAVIEGLDAAAQLEGKIFHAGTALQDERVVTSGGRVLCATALGDSVGNAQENAY
ALAAKVDWQGCFYRKDIGYRAIARERGEDQE

Specific function: De novo purine biosynthesis; second step. [C]

COG id: COG0151

COG function: function code F; Phosphoribosylamine-glycine ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Homo sapiens, GI28416899, Length=425, Percent_Identity=51.5294117647059, Blast_Score=416, Evalue=1e-116,
Organism=Homo sapiens, GI4503915, Length=424, Percent_Identity=51.6509433962264, Blast_Score=416, Evalue=1e-116,
Organism=Homo sapiens, GI209869995, Length=424, Percent_Identity=51.6509433962264, Blast_Score=416, Evalue=1e-116,
Organism=Homo sapiens, GI209869993, Length=424, Percent_Identity=51.6509433962264, Blast_Score=416, Evalue=1e-116,
Organism=Escherichia coli, GI1790438, Length=428, Percent_Identity=74.2990654205608, Blast_Score=643, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17567511, Length=426, Percent_Identity=43.1924882629108, Blast_Score=319, Evalue=2e-87,
Organism=Saccharomyces cerevisiae, GI6321203, Length=439, Percent_Identity=42.1412300683371, Blast_Score=333, Evalue=3e-92,
Organism=Drosophila melanogaster, GI62471619, Length=430, Percent_Identity=44.6511627906977, Blast_Score=340, Evalue=1e-93,
Organism=Drosophila melanogaster, GI24582400, Length=430, Percent_Identity=44.6511627906977, Blast_Score=340, Evalue=1e-93,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR020561
- InterPro:   IPR000115
- InterPro:   IPR020560
- InterPro:   IPR020559
- InterPro:   IPR020562
- InterPro:   IPR011054 [H]

Pfam domain/function: PF01071 GARS_A; PF02843 GARS_C; PF02844 GARS_N [H]

EC number: =6.3.4.13 [H]

Molecular weight: Translated: 45573; Mature: 45573

Theoretical pI: Translated: 4.51; Mature: 4.51

Prosite motif: PS50975 ATP_GRASP ; PS00184 GARS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNILIIGSGGREHALAWKVAQDPRVQKVFVAPGNAGTAIEAKCENVAIDVLALEQLADFA
CEEEEEECCCCCCEEEEEECCCCCEEEEEEECCCCCCEEEEECCCEEEHHHHHHHHHHHH
EKNVALTIVGPEVPLVAGVVDLFRSRGLDCFGPTAGAAQLEGSKAFTKDFLARHKIPTAD
HCCEEEEEECCCCHHHHHHHHHHHHCCCCCCCCCCCCCEECCCHHHHHHHHHHCCCCCCC
YQNFTEIEPALAYLREKGAPIVIKADGLAAGKGVIVAMTLAEAEDAVRDMLAGNAFGDAG
CCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCEEEEEEEHHHHHHHHHHHCCCCCCCCC
SRVVIEEFLDGEEASFIVMVDGKNVLPMATSQDHKRVGDGDSGPNTGGMGAYSPAPVVTA
CEEEEHHHCCCCCCEEEEEECCCCEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEH
EVHQRVMDLVIWPTVRGMADEGNVYTGFLYAGLMIDKAGNPKVIEFNCRFGDPETQPVML
HHHHHHHHHEECCCCCCCCCCCCEEEEEEEEEEEEECCCCCCEEEEEECCCCCCCCCEEE
RLQSSLVLLVEAALAQALDKIEAQWDPRPSLGIVLAAGGYPGDYAKGAVIEGLDAAAQLE
EEHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCCCCHHHCCCHHHHHC
GKIFHAGTALQDERVVTSGGRVLCATALGDSVGNAQENAYALAAKVDWQGCFYRKDIGYR
CEEEECCCCCCCCEEEECCCCEEEEEECCCCCCCCCCCEEEEEEEECCCCEEEECCCCHH
AIARERGEDQE
HHHHHCCCCCC
>Mature Secondary Structure
MNILIIGSGGREHALAWKVAQDPRVQKVFVAPGNAGTAIEAKCENVAIDVLALEQLADFA
CEEEEEECCCCCCEEEEEECCCCCEEEEEEECCCCCCEEEEECCCEEEHHHHHHHHHHHH
EKNVALTIVGPEVPLVAGVVDLFRSRGLDCFGPTAGAAQLEGSKAFTKDFLARHKIPTAD
HCCEEEEEECCCCHHHHHHHHHHHHCCCCCCCCCCCCCEECCCHHHHHHHHHHCCCCCCC
YQNFTEIEPALAYLREKGAPIVIKADGLAAGKGVIVAMTLAEAEDAVRDMLAGNAFGDAG
CCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCEEEEEEEHHHHHHHHHHHCCCCCCCCC
SRVVIEEFLDGEEASFIVMVDGKNVLPMATSQDHKRVGDGDSGPNTGGMGAYSPAPVVTA
CEEEEHHHCCCCCCEEEEEECCCCEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEH
EVHQRVMDLVIWPTVRGMADEGNVYTGFLYAGLMIDKAGNPKVIEFNCRFGDPETQPVML
HHHHHHHHHEECCCCCCCCCCCCEEEEEEEEEEEEECCCCCCEEEEEECCCCCCCCCEEE
RLQSSLVLLVEAALAQALDKIEAQWDPRPSLGIVLAAGGYPGDYAKGAVIEGLDAAAQLE
EEHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCCCCHHHCCCHHHHHC
GKIFHAGTALQDERVVTSGGRVLCATALGDSVGNAQENAYALAAKVDWQGCFYRKDIGYR
CEEEECCCCCCCCEEEECCCCEEEEEECCCCCCCCCCCEEEEEEEECCCCEEEECCCCHH
AIARERGEDQE
HHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12928499 [H]