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The map label for this gene is purH [H]

Identifier: 66047633

GI number: 66047633

Start: 5267706

End: 5269322

Strand: Direct

Name: purH [H]

Synonym: Psyr_4406

Alternate gene names: 66047633

Gene position: 5267706-5269322 (Clockwise)

Preceding gene: 66047632

Following gene: 66047634

Centisome position: 86.45

GC content: 62.52

Gene sequence:

>1617_bases
ATGGAAATCGAGATGACCGACCAGACTACCCGCCTGCCGATCCGCCGCGCTCTGATCAGCGTATCCGACAAGACAGGAAT
CCTTGAGTTTGCCCGCGAGCTTGAAGCGCTGGGCGTCGAGATTCTTTCCACTGGCGGTACGTTCAAGCTGCTCAAGGACA
ACGGTGTCGCGGCAGTCGAAGTGGCCGACTATACCGGCTTCGCTGAAATGATGGACGGCCGGGTCAAGACCCTGCACCCC
ATGATCCATGGCGGGATTCTGGGTCGTCGCGGCATAGACGACGCCATCATGACCGAGCACGGCATCAAGCCGATCGATCT
GGTTGCGGTCAATCTGTACCCGTTTGAAGCCACTGTCTCCAAGCCGGGCTGCGACCTGCCCACCGCCATCGAAAACATCG
ACATCGGCGGCCCGACCATGGTCCGCTCCGCGGCGAAGAACCACAAGGACGTCGCCATCGTGGTCAATGCCGGCGATTAC
GGCCAGGTCCTGGAAAGCCTCAAGGCCGGTGGCCTGACCTACGCCCAGCGCTTCGACCTGATGCTCAAGGCGTTCGAGCA
CACCGCCGCCTACGACGGCATGATCGCCAACTACCTGGGCGGCGTCGACCAGACCGCCGAAACCCTGAGTACCGAAGGCC
GCAGCCAGTTCCCGCGCACCTTCAACACCCAGTTCGTCAAGGCTCAGGAAATGCGCTACGGCGAGAACCCGCACCAGAGT
GCCGCGTTCTACGTCGAAGCCAAACCTGCCGAGGTCGGCATCGCCACCGCCACCCAGTTGCAAGGCAAGGAGCTGTCGTT
CAACAACGTGGCCGATACCGATGCCGCACTGGAATGCGTCAAGAGCTTCGTCAAACCGGCCTGCGTGATCGTCAAGCACG
CCAACCCCTGCGGTGTGGCGGTCTGCCCGGATGATGAAGGCGGCATTCGTCAGGCTTACGAACTGGCCTACGCCACCGAT
TCCGAGTCGGCATTTGGCGGCATCATCGCCTTCAACCGCGAGCTGGACGCTGCAACCGCCAAGGCCATCGTCGAGCGTCA
GTTCGTCGAAGTGATCATCGCACCGAGCGTCAGCGCCGAAGCCCGCGCCATCGTCGCCAGCAAAGCCAACGTGCGCCTGC
TGACCAGCGGCCAGTGGAGCGAGCGCCTGCCTGCGTGGGACTACAAGCGTGTCAATGGCGGCCTGCTGGTACAGAGCCGT
GACATCGGCATGATCACCGAAGCCGACCTCAAGGTCGTCACCCGGCGTGCGCCGACCGAACAGGAAATGCATGACCTGAT
CTTCGCCTGGAAAGTCGCCAAGTACGTCAAGTCCAACGCCATCGTCTACGCCCGCAACCGCCAGACCGTCGGCGTCGGTG
CAGGCCAGATGAGCCGCGTGAACTCGGCACGTATCGCTGCCATCAAGGCTGAACACGCCGGGCTGCAGGTTCAGGGCGCG
GTCATGGCCTCCGATGCCTTCTTCCCGTTCCGTGACGGCATCGACAACGCGGCCAAGGTCGGCATCACCGCTGTCATACA
GCCAGGCGGCTCGATGCGTGACAATGAAGTGATCGCTGCGGCCGACGAAGCCGGCATCGCGATGGTGTTTACCGGCATGC
GCCATTTCAGGCACTAA

Upstream 100 bases:

>100_bases
GCAAGAAACTCAAACAGTACGATCTGCTTTAAAGCATTCAAAACAGAAAAGGCGACCTTGCACCGCGGTCGCTTTTTTTT
GCTGACTTCTTTTGCTTTTG

Downstream 100 bases:

>100_bases
AGGCAGCTTCAAGCTTCAAGCTTCAAGCTTCAAGCTTCAAGCTTCAAGCTTCAAGCTAAAGCCTAGCGTACTAACCATTC
AACGCCACAAGCTAAACACT

Product: bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase

Products: NA

Alternate protein names: Phosphoribosylaminoimidazolecarboxamide formyltransferase; AICAR transformylase; IMP cyclohydrolase; ATIC; IMP synthase; Inosinicase [H]

Number of amino acids: Translated: 538; Mature: 538

Protein sequence:

>538_residues
MEIEMTDQTTRLPIRRALISVSDKTGILEFARELEALGVEILSTGGTFKLLKDNGVAAVEVADYTGFAEMMDGRVKTLHP
MIHGGILGRRGIDDAIMTEHGIKPIDLVAVNLYPFEATVSKPGCDLPTAIENIDIGGPTMVRSAAKNHKDVAIVVNAGDY
GQVLESLKAGGLTYAQRFDLMLKAFEHTAAYDGMIANYLGGVDQTAETLSTEGRSQFPRTFNTQFVKAQEMRYGENPHQS
AAFYVEAKPAEVGIATATQLQGKELSFNNVADTDAALECVKSFVKPACVIVKHANPCGVAVCPDDEGGIRQAYELAYATD
SESAFGGIIAFNRELDAATAKAIVERQFVEVIIAPSVSAEARAIVASKANVRLLTSGQWSERLPAWDYKRVNGGLLVQSR
DIGMITEADLKVVTRRAPTEQEMHDLIFAWKVAKYVKSNAIVYARNRQTVGVGAGQMSRVNSARIAAIKAEHAGLQVQGA
VMASDAFFPFRDGIDNAAKVGITAVIQPGGSMRDNEVIAAADEAGIAMVFTGMRHFRH

Sequences:

>Translated_538_residues
MEIEMTDQTTRLPIRRALISVSDKTGILEFARELEALGVEILSTGGTFKLLKDNGVAAVEVADYTGFAEMMDGRVKTLHP
MIHGGILGRRGIDDAIMTEHGIKPIDLVAVNLYPFEATVSKPGCDLPTAIENIDIGGPTMVRSAAKNHKDVAIVVNAGDY
GQVLESLKAGGLTYAQRFDLMLKAFEHTAAYDGMIANYLGGVDQTAETLSTEGRSQFPRTFNTQFVKAQEMRYGENPHQS
AAFYVEAKPAEVGIATATQLQGKELSFNNVADTDAALECVKSFVKPACVIVKHANPCGVAVCPDDEGGIRQAYELAYATD
SESAFGGIIAFNRELDAATAKAIVERQFVEVIIAPSVSAEARAIVASKANVRLLTSGQWSERLPAWDYKRVNGGLLVQSR
DIGMITEADLKVVTRRAPTEQEMHDLIFAWKVAKYVKSNAIVYARNRQTVGVGAGQMSRVNSARIAAIKAEHAGLQVQGA
VMASDAFFPFRDGIDNAAKVGITAVIQPGGSMRDNEVIAAADEAGIAMVFTGMRHFRH
>Mature_538_residues
MEIEMTDQTTRLPIRRALISVSDKTGILEFARELEALGVEILSTGGTFKLLKDNGVAAVEVADYTGFAEMMDGRVKTLHP
MIHGGILGRRGIDDAIMTEHGIKPIDLVAVNLYPFEATVSKPGCDLPTAIENIDIGGPTMVRSAAKNHKDVAIVVNAGDY
GQVLESLKAGGLTYAQRFDLMLKAFEHTAAYDGMIANYLGGVDQTAETLSTEGRSQFPRTFNTQFVKAQEMRYGENPHQS
AAFYVEAKPAEVGIATATQLQGKELSFNNVADTDAALECVKSFVKPACVIVKHANPCGVAVCPDDEGGIRQAYELAYATD
SESAFGGIIAFNRELDAATAKAIVERQFVEVIIAPSVSAEARAIVASKANVRLLTSGQWSERLPAWDYKRVNGGLLVQSR
DIGMITEADLKVVTRRAPTEQEMHDLIFAWKVAKYVKSNAIVYARNRQTVGVGAGQMSRVNSARIAAIKAEHAGLQVQGA
VMASDAFFPFRDGIDNAAKVGITAVIQPGGSMRDNEVIAAADEAGIAMVFTGMRHFRH

Specific function: De novo purine biosynthesis; ninth step. De novo purine biosynthesis; tenth step. [C]

COG id: COG0138

COG function: function code F; AICAR transformylase/IMP cyclohydrolase PurH (only IMP cyclohydrolase domain in Aful)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purH family [H]

Homologues:

Organism=Homo sapiens, GI20127454, Length=512, Percent_Identity=37.109375, Blast_Score=267, Evalue=2e-71,
Organism=Escherichia coli, GI1790439, Length=530, Percent_Identity=67.5471698113208, Blast_Score=740, Evalue=0.0,
Organism=Caenorhabditis elegans, GI71985564, Length=521, Percent_Identity=32.6295585412668, Blast_Score=249, Evalue=2e-66,
Organism=Caenorhabditis elegans, GI71985574, Length=354, Percent_Identity=26.271186440678, Blast_Score=96, Evalue=5e-20,
Organism=Caenorhabditis elegans, GI71985556, Length=63, Percent_Identity=50.7936507936508, Blast_Score=77, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6323768, Length=496, Percent_Identity=35.4838709677419, Blast_Score=250, Evalue=5e-67,
Organism=Saccharomyces cerevisiae, GI6323056, Length=496, Percent_Identity=35.0806451612903, Blast_Score=246, Evalue=6e-66,
Organism=Drosophila melanogaster, GI24649832, Length=507, Percent_Identity=36.2919132149901, Blast_Score=259, Evalue=5e-69,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002695
- InterPro:   IPR013982
- InterPro:   IPR016193
- InterPro:   IPR011607 [H]

Pfam domain/function: PF01808 AICARFT_IMPCHas; PF02142 MGS [H]

EC number: =2.1.2.3; =3.5.4.10 [H]

Molecular weight: Translated: 57960; Mature: 57960

Theoretical pI: Translated: 5.84; Mature: 5.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEIEMTDQTTRLPIRRALISVSDKTGILEFARELEALGVEILSTGGTFKLLKDNGVAAVE
CEEEECCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCEEEEECCCEEEEEECCCEEEEE
VADYTGFAEMMDGRVKTLHPMIHGGILGRRGIDDAIMTEHGIKPIDLVAVNLYPFEATVS
EECCCCHHHHHCCHHHHHHHHHHCCCCCCCCCCHHHHHHCCCCCEEEEEEEEEEEEEECC
KPGCDLPTAIENIDIGGPTMVRSAAKNHKDVAIVVNAGDYGQVLESLKAGGLTYAQRFDL
CCCCCCCHHHHCCCCCCHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHCCCCHHHHHHHH
MLKAFEHTAAYDGMIANYLGGVDQTAETLSTEGRSQFPRTFNTQFVKAQEMRYGENPHQS
HHHHHHHHHHHCCHHHHHHCCCCHHHHHHHCCCHHHCCCCCCCHHEEHHHHHCCCCCCCC
AAFYVEAKPAEVGIATATQLQGKELSFNNVADTDAALECVKSFVKPACVIVKHANPCGVA
EEEEEEECCCCCCEEEECCCCCCCCCCCCCCCHHHHHHHHHHHCCCEEEEEECCCCCEEE
VCPDDEGGIRQAYELAYATDSESAFGGIIAFNRELDAATAKAIVERQFVEVIIAPSVSAE
ECCCCCCCHHHHHHEEEECCCCCCCCEEEEECCCCCHHHHHHHHHHHHEEEEEECCCCCC
ARAIVASKANVRLLTSGQWSERLPAWDYKRVNGGLLVQSRDIGMITEADLKVVTRRAPTE
CEEEEECCCCEEEEECCCCHHCCCCCCCEECCCCEEEEECCCCEEECCCHHEEECCCCCH
QEMHDLIFAWKVAKYVKSNAIVYARNRQTVGVGAGQMSRVNSARIAAIKAEHAGLQVQGA
HHHHHHHHHHHHHHHHHCCEEEEEECCEEEEECCCHHHHCCCCEEEEEEECCCCEEEEEE
VMASDAFFPFRDGIDNAAKVGITAVIQPGGSMRDNEVIAAADEAGIAMVFTGMRHFRH
EEECCCCCCHHCCCCCHHHCCEEEEECCCCCCCCCCEEEECCCCCEEHEEHHHHHHCC
>Mature Secondary Structure
MEIEMTDQTTRLPIRRALISVSDKTGILEFARELEALGVEILSTGGTFKLLKDNGVAAVE
CEEEECCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCEEEEECCCEEEEEECCCEEEEE
VADYTGFAEMMDGRVKTLHPMIHGGILGRRGIDDAIMTEHGIKPIDLVAVNLYPFEATVS
EECCCCHHHHHCCHHHHHHHHHHCCCCCCCCCCHHHHHHCCCCCEEEEEEEEEEEEEECC
KPGCDLPTAIENIDIGGPTMVRSAAKNHKDVAIVVNAGDYGQVLESLKAGGLTYAQRFDL
CCCCCCCHHHHCCCCCCHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHCCCCHHHHHHHH
MLKAFEHTAAYDGMIANYLGGVDQTAETLSTEGRSQFPRTFNTQFVKAQEMRYGENPHQS
HHHHHHHHHHHCCHHHHHHCCCCHHHHHHHCCCHHHCCCCCCCHHEEHHHHHCCCCCCCC
AAFYVEAKPAEVGIATATQLQGKELSFNNVADTDAALECVKSFVKPACVIVKHANPCGVA
EEEEEEECCCCCCEEEECCCCCCCCCCCCCCCHHHHHHHHHHHCCCEEEEEECCCCCEEE
VCPDDEGGIRQAYELAYATDSESAFGGIIAFNRELDAATAKAIVERQFVEVIIAPSVSAE
ECCCCCCCHHHHHHEEEECCCCCCCCEEEEECCCCCHHHHHHHHHHHHEEEEEECCCCCC
ARAIVASKANVRLLTSGQWSERLPAWDYKRVNGGLLVQSRDIGMITEADLKVVTRRAPTE
CEEEEECCCCEEEEECCCCHHCCCCCCCEECCCCEEEEECCCCEEECCCHHEEECCCCCH
QEMHDLIFAWKVAKYVKSNAIVYARNRQTVGVGAGQMSRVNSARIAAIKAEHAGLQVQGA
HHHHHHHHHHHHHHHHHCCEEEEEECCEEEEECCCHHHHCCCCEEEEEEECCCCEEEEEE
VMASDAFFPFRDGIDNAAKVGITAVIQPGGSMRDNEVIAAADEAGIAMVFTGMRHFRH
EEECCCCCCHHCCCCCHHHCCEEEEECCCCCCCCCCEEEECCCCCEEHEEHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA