Click here to switch to the map view.

The map label for this gene is dusB [H]

Identifier: 66047631

GI number: 66047631

Start: 5266307

End: 5267320

Strand: Direct

Name: dusB [H]

Synonym: Psyr_4404

Alternate gene names: 66047631

Gene position: 5266307-5267320 (Clockwise)

Preceding gene: 66047630

Following gene: 66047632

Centisome position: 86.42

GC content: 60.16

Gene sequence:

>1014_bases
ATGTCGGCAGTACGCATCGGCCCATATACAGTGCAAAACGGCTTGATCCTCGCCCCGATGGCGGGCGTCACCGACCAGCC
TTTCCGTCAGCTGTGCCGAAGACTCGGTGCCGGCCTGGTGGTTTCGGAGATGGTCACCAGTGACATGAGCCTGTGGAACA
GCCGCAAATCGCGTCTGCGCATGATCCACGAAGGTGATCCCGAGCCGCGCTCGGTACAGATCGCCGGTGGTGATCCGCAG
ATGCTGGCAGACGCAGCACGTGCCAATGTCGAACTGGGCGCCCAGATCATCGACATCAACATGGGTTGCCCGGCCAAGAA
AGTCTGTAACAAGGCGGCTGGCTCGGCGCTGTTGAAGGACGAGCAACTGGTCAACGACATCCTGCAGGCCGTCGTGGCTG
CAGTCGATGTACCGGTCACGCTGAAGATCCGCACCGGATGGGATCGCGACAACCGAAATGGCCTGACAGTGGCGAAAATC
GCCGAGCAGGCGGGCATCACGGCACTGGCGGTACACGGGCGAACCCGTGCCGACCTGTACACCGGCGAGGCCGAGTACGA
CACCATTGCCCTGATCAAACAGGCCGTGTCGATACCGGTCTTTGCCAATGGCGACATCGATTCACCGGAAAAGGCCCGGC
ACGTGCTGAAGGCTACCGGTGCAGACGGATTACTGATTGGCAGGGCCGCACAGGGGCGTCCCTGGATTTTTCGCGAGATA
GAACACTTCCTGCGTACCGGAGAAACACTCCCGGCGCCGCAGTCGAGTGAAGTGGAACGCATTCTGCTTGAGCATCTGGC
AGCGCTCCACGTTTTCTATGGAGACGTGATGGGCGTGCGCATTGCACGCAAACACGTCAGTTGGTATCTCGCAACGCTGC
CGGGCGCCAGAGAGTTTCGCGCCCACTTCAATCGTTTGGAAGATACGGAAGCACAGTGCGCCAACGTCCGGGAGTTCTTC
AGCCAAGGCTGCAAGGGCCCGGATAACGAGAATGATAAAGAGGTGGCCGCATGA

Upstream 100 bases:

>100_bases
GACGGTGGCGCATGCGGCTGGCGGGATGAGCCGTTGCAGACCGGGTGAAACGGTTTTTACACGGCCTGCGGATGATTTGG
CCTCCACAACAGGTAAGGCT

Downstream 100 bases:

>100_bases
CGATGATGACCGAGACTTTAGTGAGTGGAACAACACCCGTGAGCGACAACGTCAATTTGAAACAGCACCTCAACACGCCG
AGCGAAGAGGGTCAGACCCT

Product: dihydrouridine synthase TIM-barrel protein nifR3

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 337; Mature: 336

Protein sequence:

>337_residues
MSAVRIGPYTVQNGLILAPMAGVTDQPFRQLCRRLGAGLVVSEMVTSDMSLWNSRKSRLRMIHEGDPEPRSVQIAGGDPQ
MLADAARANVELGAQIIDINMGCPAKKVCNKAAGSALLKDEQLVNDILQAVVAAVDVPVTLKIRTGWDRDNRNGLTVAKI
AEQAGITALAVHGRTRADLYTGEAEYDTIALIKQAVSIPVFANGDIDSPEKARHVLKATGADGLLIGRAAQGRPWIFREI
EHFLRTGETLPAPQSSEVERILLEHLAALHVFYGDVMGVRIARKHVSWYLATLPGAREFRAHFNRLEDTEAQCANVREFF
SQGCKGPDNENDKEVAA

Sequences:

>Translated_337_residues
MSAVRIGPYTVQNGLILAPMAGVTDQPFRQLCRRLGAGLVVSEMVTSDMSLWNSRKSRLRMIHEGDPEPRSVQIAGGDPQ
MLADAARANVELGAQIIDINMGCPAKKVCNKAAGSALLKDEQLVNDILQAVVAAVDVPVTLKIRTGWDRDNRNGLTVAKI
AEQAGITALAVHGRTRADLYTGEAEYDTIALIKQAVSIPVFANGDIDSPEKARHVLKATGADGLLIGRAAQGRPWIFREI
EHFLRTGETLPAPQSSEVERILLEHLAALHVFYGDVMGVRIARKHVSWYLATLPGAREFRAHFNRLEDTEAQCANVREFF
SQGCKGPDNENDKEVAA
>Mature_336_residues
SAVRIGPYTVQNGLILAPMAGVTDQPFRQLCRRLGAGLVVSEMVTSDMSLWNSRKSRLRMIHEGDPEPRSVQIAGGDPQM
LADAARANVELGAQIIDINMGCPAKKVCNKAAGSALLKDEQLVNDILQAVVAAVDVPVTLKIRTGWDRDNRNGLTVAKIA
EQAGITALAVHGRTRADLYTGEAEYDTIALIKQAVSIPVFANGDIDSPEKARHVLKATGADGLLIGRAAQGRPWIFREIE
HFLRTGETLPAPQSSEVERILLEHLAALHVFYGDVMGVRIARKHVSWYLATLPGAREFRAHFNRLEDTEAQCANVREFFS
QGCKGPDNENDKEVAA

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family. DusB subfamily [H]

Homologues:

Organism=Homo sapiens, GI239788483, Length=241, Percent_Identity=32.3651452282158, Blast_Score=117, Evalue=1e-26,
Organism=Homo sapiens, GI239788462, Length=241, Percent_Identity=32.3651452282158, Blast_Score=116, Evalue=3e-26,
Organism=Homo sapiens, GI31742496, Length=227, Percent_Identity=34.3612334801762, Blast_Score=115, Evalue=8e-26,
Organism=Homo sapiens, GI40807366, Length=230, Percent_Identity=30.8695652173913, Blast_Score=101, Evalue=1e-21,
Organism=Homo sapiens, GI8923374, Length=246, Percent_Identity=31.7073170731707, Blast_Score=91, Evalue=1e-18,
Organism=Escherichia coli, GI1789660, Length=317, Percent_Identity=61.198738170347, Blast_Score=387, Evalue=1e-109,
Organism=Escherichia coli, GI1788462, Length=244, Percent_Identity=34.4262295081967, Blast_Score=123, Evalue=2e-29,
Organism=Escherichia coli, GI145693211, Length=302, Percent_Identity=26.4900662251656, Blast_Score=80, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI25144369, Length=222, Percent_Identity=33.3333333333333, Blast_Score=100, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17543114, Length=234, Percent_Identity=33.3333333333333, Blast_Score=99, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17510279, Length=252, Percent_Identity=31.7460317460317, Blast_Score=89, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI17507177, Length=242, Percent_Identity=30.1652892561983, Blast_Score=87, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6323560, Length=230, Percent_Identity=30.8695652173913, Blast_Score=93, Evalue=6e-20,
Organism=Saccharomyces cerevisiae, GI6323433, Length=267, Percent_Identity=27.3408239700375, Blast_Score=89, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6323437, Length=230, Percent_Identity=28.2608695652174, Blast_Score=79, Evalue=8e-16,
Organism=Drosophila melanogaster, GI24580595, Length=290, Percent_Identity=30.6896551724138, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI19920448, Length=290, Percent_Identity=30.6896551724138, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI24585320, Length=279, Percent_Identity=29.3906810035842, Blast_Score=112, Evalue=3e-25,
Organism=Drosophila melanogaster, GI19921524, Length=311, Percent_Identity=27.6527331189711, Blast_Score=101, Evalue=7e-22,
Organism=Drosophila melanogaster, GI45549423, Length=263, Percent_Identity=31.9391634980989, Blast_Score=91, Evalue=9e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004652
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 36707; Mature: 36576

Theoretical pI: Translated: 6.94; Mature: 6.94

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAVRIGPYTVQNGLILAPMAGVTDQPFRQLCRRLGAGLVVSEMVTSDMSLWNSRKSRLR
CCCEEECCEEECCCEEEECCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
MIHEGDPEPRSVQIAGGDPQMLADAARANVELGAQIIDINMGCPAKKVCNKAAGSALLKD
HHHCCCCCCCEEEEECCCHHHHHHHHHCCCEECCEEEEECCCCCHHHHHHHHHCHHHHHH
EQLVNDILQAVVAAVDVPVTLKIRTGWDRDNRNGLTVAKIAEQAGITALAVHGRTRADLY
HHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCHHHHHHHHCCCEEEEECCCCCCCEE
TGEAEYDTIALIKQAVSIPVFANGDIDSPEKARHVLKATGADGLLIGRAAQGRPWIFREI
CCCCCHHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHHCCCCCEEEECCCCCCCHHHHHH
EHFLRTGETLPAPQSSEVERILLEHLAALHVFYGDVMGVRIARKHVSWYLATLPGAREFR
HHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCHHHHH
AHFNRLEDTEAQCANVREFFSQGCKGPDNENDKEVAA
HHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SAVRIGPYTVQNGLILAPMAGVTDQPFRQLCRRLGAGLVVSEMVTSDMSLWNSRKSRLR
CCEEECCEEECCCEEEECCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
MIHEGDPEPRSVQIAGGDPQMLADAARANVELGAQIIDINMGCPAKKVCNKAAGSALLKD
HHHCCCCCCCEEEEECCCHHHHHHHHHCCCEECCEEEEECCCCCHHHHHHHHHCHHHHHH
EQLVNDILQAVVAAVDVPVTLKIRTGWDRDNRNGLTVAKIAEQAGITALAVHGRTRADLY
HHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCHHHHHHHHCCCEEEEECCCCCCCEE
TGEAEYDTIALIKQAVSIPVFANGDIDSPEKARHVLKATGADGLLIGRAAQGRPWIFREI
CCCCCHHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHHCCCCCEEEECCCCCCCHHHHHH
EHFLRTGETLPAPQSSEVERILLEHLAALHVFYGDVMGVRIARKHVSWYLATLPGAREFR
HHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCHHHHH
AHFNRLEDTEAQCANVREFFSQGCKGPDNENDKEVAA
HHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12928499 [H]