Click here to switch to the map view.

The map label for this gene is tfdA [H]

Identifier: 66047544

GI number: 66047544

Start: 5166566

End: 5167579

Strand: Reverse

Name: tfdA [H]

Synonym: Psyr_4317

Alternate gene names: 66047544

Gene position: 5167579-5166566 (Counterclockwise)

Preceding gene: 66047546

Following gene: 66047543

Centisome position: 84.8

GC content: 44.97

Gene sequence:

>1014_bases
ATGGGTTTAAAATTATCAAGTGTTCCTTTTGAAATATCATACCCGGCTCAGAGTTTTGGCGTCGAAATCGAGAATTTAAA
ACTACACAAAAATCTGTCGTTTGACACAATCGCAGCGATAAAAAAATGCTTGGCATATCGCGGGTTTGTGTTATTCAGGA
ATCAAAATCTTACACGAAGAGAGCAAGTCGCAGTCACGCGACTGCTGGGCAATCCGAACCTAAAGTCACACTCTTGGGCG
CCGCAGATAGAGACAACTACTTTTGGTGATGACGAGGTGATTCCTAATGGGCTGCCGCTCATTAATCATGGGGAGATTTT
GTATTTTGTGAATGGCCCGGATTTTTGCGACAAAACTCAGGATGAGCATGCGATATGGGATGAGAAAGACAATCATACCA
AGGGTAAGGGAACTTCGTGTTGGCACACTGGTGACTCGGAAGCCATAAACGTTGAAACGATTAATTGCTTATATGCAGAG
CTGGCTGCCGCACAAGGCGGAGCAACGTTATTTTGCGACACCGTTGCCGCCTATAATGACTTGGAAGACAGCCTGAAAAA
GCGTATTGATAATCTTCGAGTGCTTCACTACTTTGTCGATCCTCAGCGTACTGAACCGGTCAACCAACCATTAGTTAAAA
CCAATCCGATCACCCGGCAAAAATATCTTTATCTGAACTACAACACTATGGAGCGTGTTGAAGGGCTGAGTAAGTCAGAG
AGCTACAGGCTCTTAAAGTTTCTGTTTGATCATCAAATTAAAGATCAATACGTTTATGAGCACTTTTGGAAGCAGGACGA
TTTTCTGATCTGGAATTGCAATGGCACCATGCACAAGCGTGGTGCGCTGGTCTCGAACCACAACAGGATCATGCGTCGGA
CTCAAGCTATTATTCCGGAGTTTACCAGTTACTACGAACGGGATAAAAGCCTGGCAGTGACCAGTCATGATAATGAAGAC
TGGCATCCATATCCTTTTGAACGCGCAGAATTGGAGTCGGCCGGGTCGGCTTGA

Upstream 100 bases:

>100_bases
CTGAATAGGAGGGCGGTGAGGTGATTCTTACAGGCCCGAGCTTCAAGGTATTTATAAGCGTTAACTCAATCAGTGTAGAT
TAAAATTCTGGGGGGTTGTC

Downstream 100 bases:

>100_bases
TCTATTCGTTGAGGGTGCGTTTAAAAGTCCCTCCGTTGCGGTGGAATGTGCAATGGCTATGAAAGAGTGAATACATGAAA
GAAAAAATCAAATCCTATCT

Product: taurine catabolism dioxygenase

Products: Aminoacetaldehyde; CO2; H+; Sulfite; Succinate [C]

Alternate protein names: 2,4-D dioxygenase [H]

Number of amino acids: Translated: 337; Mature: 336

Protein sequence:

>337_residues
MGLKLSSVPFEISYPAQSFGVEIENLKLHKNLSFDTIAAIKKCLAYRGFVLFRNQNLTRREQVAVTRLLGNPNLKSHSWA
PQIETTTFGDDEVIPNGLPLINHGEILYFVNGPDFCDKTQDEHAIWDEKDNHTKGKGTSCWHTGDSEAINVETINCLYAE
LAAAQGGATLFCDTVAAYNDLEDSLKKRIDNLRVLHYFVDPQRTEPVNQPLVKTNPITRQKYLYLNYNTMERVEGLSKSE
SYRLLKFLFDHQIKDQYVYEHFWKQDDFLIWNCNGTMHKRGALVSNHNRIMRRTQAIIPEFTSYYERDKSLAVTSHDNED
WHPYPFERAELESAGSA

Sequences:

>Translated_337_residues
MGLKLSSVPFEISYPAQSFGVEIENLKLHKNLSFDTIAAIKKCLAYRGFVLFRNQNLTRREQVAVTRLLGNPNLKSHSWA
PQIETTTFGDDEVIPNGLPLINHGEILYFVNGPDFCDKTQDEHAIWDEKDNHTKGKGTSCWHTGDSEAINVETINCLYAE
LAAAQGGATLFCDTVAAYNDLEDSLKKRIDNLRVLHYFVDPQRTEPVNQPLVKTNPITRQKYLYLNYNTMERVEGLSKSE
SYRLLKFLFDHQIKDQYVYEHFWKQDDFLIWNCNGTMHKRGALVSNHNRIMRRTQAIIPEFTSYYERDKSLAVTSHDNED
WHPYPFERAELESAGSA
>Mature_336_residues
GLKLSSVPFEISYPAQSFGVEIENLKLHKNLSFDTIAAIKKCLAYRGFVLFRNQNLTRREQVAVTRLLGNPNLKSHSWAP
QIETTTFGDDEVIPNGLPLINHGEILYFVNGPDFCDKTQDEHAIWDEKDNHTKGKGTSCWHTGDSEAINVETINCLYAEL
AAAQGGATLFCDTVAAYNDLEDSLKKRIDNLRVLHYFVDPQRTEPVNQPLVKTNPITRQKYLYLNYNTMERVEGLSKSES
YRLLKFLFDHQIKDQYVYEHFWKQDDFLIWNCNGTMHKRGALVSNHNRIMRRTQAIIPEFTSYYERDKSLAVTSHDNEDW
HPYPFERAELESAGSA

Specific function: Involved in degradation of the herbicide 2,4- dichlorophenoxyacetic acid (2,4-D). Is also able to degrade 2- methyl-4-chlorophenoxyacetic acid and 3-chlorobenzoic acid [H]

COG id: COG2175

COG function: function code Q; Probable taurine catabolism dioxygenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tfdA dioxygenase family [H]

Homologues:

Organism=Escherichia coli, GI1786565, Length=314, Percent_Identity=25.1592356687898, Blast_Score=75, Evalue=5e-15,
Organism=Saccharomyces cerevisiae, GI6322971, Length=304, Percent_Identity=23.6842105263158, Blast_Score=65, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003819 [H]

Pfam domain/function: PF02668 TauD [H]

EC number: 1.14.11.17 [C]

Molecular weight: Translated: 38832; Mature: 38701

Theoretical pI: Translated: 6.32; Mature: 6.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGLKLSSVPFEISYPAQSFGVEIENLKLHKNLSFDTIAAIKKCLAYRGFVLFRNQNLTRR
CCCCCCCCCEEEECCHHHHCEEEEEEEEECCCCHHHHHHHHHHHHHCCEEEEECCCCCHH
EQVAVTRLLGNPNLKSHSWAPQIETTTFGDDEVIPNGLPLINHGEILYFVNGPDFCDKTQ
HHHHHHHHHCCCCCCCCCCCCCCEEEECCCCCCCCCCCCEEECCCEEEEECCCCCCCCCC
DEHAIWDEKDNHTKGKGTSCWHTGDSEAINVETINCLYAELAAAQGGATLFCDTVAAYND
CCCCCCCCCCCCCCCCCCEEEECCCCCEEEEEHHHHHHHHHHHCCCCCEEEEHHHHHHHH
LEDSLKKRIDNLRVLHYFVDPQRTEPVNQPLVKTNPITRQKYLYLNYNTMERVEGLSKSE
HHHHHHHHHHCEEEEEEEECCCCCCCCCCCCCCCCCCCCEEEEEEECCHHHHHHCCCCCH
SYRLLKFLFDHQIKDQYVYEHFWKQDDFLIWNCNGTMHKRGALVSNHNRIMRRTQAIIPE
HHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCEECCCHHHHHHHHHHHHH
FTSYYERDKSLAVTSHDNEDWHPYPFERAELESAGSA
HHHHHHCCCCEEEEECCCCCCCCCCCCHHHHHCCCCC
>Mature Secondary Structure 
GLKLSSVPFEISYPAQSFGVEIENLKLHKNLSFDTIAAIKKCLAYRGFVLFRNQNLTRR
CCCCCCCCEEEECCHHHHCEEEEEEEEECCCCHHHHHHHHHHHHHCCEEEEECCCCCHH
EQVAVTRLLGNPNLKSHSWAPQIETTTFGDDEVIPNGLPLINHGEILYFVNGPDFCDKTQ
HHHHHHHHHCCCCCCCCCCCCCCEEEECCCCCCCCCCCCEEECCCEEEEECCCCCCCCCC
DEHAIWDEKDNHTKGKGTSCWHTGDSEAINVETINCLYAELAAAQGGATLFCDTVAAYND
CCCCCCCCCCCCCCCCCCEEEECCCCCEEEEEHHHHHHHHHHHCCCCCEEEEHHHHHHHH
LEDSLKKRIDNLRVLHYFVDPQRTEPVNQPLVKTNPITRQKYLYLNYNTMERVEGLSKSE
HHHHHHHHHHCEEEEEEEECCCCCCCCCCCCCCCCCCCCEEEEEEECCHHHHHHCCCCCH
SYRLLKFLFDHQIKDQYVYEHFWKQDDFLIWNCNGTMHKRGALVSNHNRIMRRTQAIIPE
HHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCEECCCHHHHHHHHHHHHH
FTSYYERDKSLAVTSHDNEDWHPYPFERAELESAGSA
HHHHHHCCCCEEEEECCCCCCCCCCCCHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Ascorbate. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2-Oxoglutarate; O2; Taurine [C]

Specific reaction: 2-Oxoglutarate + O2 + Taurine --> Aminoacetaldehyde + CO2 + H+ + Sulfite + Succinate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3036764; 10777523; 11457355; 11955067 [H]