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The map label for this gene is garD [H]

Identifier: 66047452

GI number: 66047452

Start: 5031323

End: 5032876

Strand: Reverse

Name: garD [H]

Synonym: Psyr_4225

Alternate gene names: 66047452

Gene position: 5032876-5031323 (Counterclockwise)

Preceding gene: 66047453

Following gene: 66047451

Centisome position: 82.59

GC content: 61.71

Gene sequence:

>1554_bases
ATGAACCTGATTCAACATGCCGATTCGCCACGCTATATCCGGCTGCATCCGCTGGATAACGTCGTGGTCGTGGTCAACGA
CCAGGGTGTGCCGGCCGGAACCGAATTCGACAACGGCCTGGTCACTGTCGATAACGTACCGCAGAGCCATAAGGTCAGTA
CCGTTGACCTGGCCGTGGGCGAGGCCGTGATTCGTTACGGCCACACCATCGGCTATGCACTGCAACCGATACCGCGCGGC
AGTTGGGTCAGGGAAGACCAGTTGCGCATGCCTTCCGCGCCTGCGCTCGACAGCCTGCCGATGTCCGATGCCGTGCCGGA
AAAGCAGGCCCCGCTCGAGGGTTACACCTTCGAAGGCTATCGCAACGCCGACGGCACCGTGGGCACTCGCAACATTCTCG
GTATCACCACCACCGTGCAGTGCGTGACCGGTGTGCTCGACCATGCGGTCAAGCGCATCCGTGACGAGTTGTTGCCCAAG
TACCCGAACGTCGACGACGTCGTGGCGCTGACCCACAGCTACGGCTGCGGCGTGGCGATCACTGCCACTGATGCTTACAT
CCCGATTCGTACGGTACGTAACCTGGCACGCAACCCGAATCTGGGCGGCGAGGCACTGGTGATCAGCTTGGGCTGTGAGA
AGTTGCAGGCCGGGCAGGTCATGCATGAGGGCGACAGCTCGGTGGACCTCAGCGAACCCTGGCTATATCGCCTGCAGGAC
TCCAGCCACGGCTTCAACGAAATGATCGAACAGATCATGGAGCTGGCCGAGACGCGTTTGAAAAAGCTGGATCTGCGGCG
TCGCGAGACGGTTCCCGCTTCGGAGCTGATTCTGGGCATGCAATGCGGCGGCAGTGATGCGTTTTCCGGCATTACCGCCA
ACCCGGCATTGGGTTACGCCTCGGACCTGCTGCTTCGCGCCGGTGCAACGGTGATGTTTTCCGAAGTGACCGAAGTGCGC
GATGCCATCTACCTGCTGACTTCACGCGCACAGGATCAGGACGTCGCGCAGGCGCTGGTGCGGGAGATGGACTGGTACGA
CCGCTATCTGGCCAAAGGCGAGGCTGATCGCAGCGCCAACACCACGCCGGGCAACAAGAAGGGCGGGTTGTCGAACATCG
TCGAGAAGTCTCTGGGATCGATCGTCAAGTCAGGCAGCAGTGCCATCAACGGCGTACTCGGCCCTGGCGAGCGGGTCAGC
GGCAAGGGGTTGATCTTCTGCGCGACACCGGCCAGTGACTTCGTCTGCGGCACCTTGCAACTGGCTGCCGGCATGAACCT
GCATGTATTCACCACCGGGCGCGGTACTCCGTATGGTCTGGCAATGGCGCCGGTAGTGAAGGTCTCGACCCGCACCGAAC
TGGCCCAGCGCTGGCCTGACCTGATCGATATAGATGCCGGGCGCATCGCGACCGGACGCGCGAGCATCGAGGAGCTGGGC
TGGGAGCTTTTCCACTTCTACCTGGACGTGGCCAGCGGCAAGAAGAAGACCTGGACCGAGCATTACCGGCTGCACAACGA
CATCACGCTCTTCAACCCGGCACCGATTACCTGA

Upstream 100 bases:

>100_bases
GAAATCAAGCGTGTGGAATTGAAAGAGCCGCCTGCCAAGGGGCCATTGCTGAACGACTCGGTCAGCGACCTTTCCGAAGC
AAAATCCTGAGGAAACCGTG

Downstream 100 bases:

>100_bases
CTGGAGTGGGACGCGGAGCTTCACGAACGGCATGCCCACGCTGGAGCGTGAGGAACGATATGCGGGTGAATACGCCTATC
GTGCTCATGCTCTGCCTGGG

Product: galactarate dehydratase

Products: NA

Alternate protein names: GalcD [H]

Number of amino acids: Translated: 517; Mature: 517

Protein sequence:

>517_residues
MNLIQHADSPRYIRLHPLDNVVVVVNDQGVPAGTEFDNGLVTVDNVPQSHKVSTVDLAVGEAVIRYGHTIGYALQPIPRG
SWVREDQLRMPSAPALDSLPMSDAVPEKQAPLEGYTFEGYRNADGTVGTRNILGITTTVQCVTGVLDHAVKRIRDELLPK
YPNVDDVVALTHSYGCGVAITATDAYIPIRTVRNLARNPNLGGEALVISLGCEKLQAGQVMHEGDSSVDLSEPWLYRLQD
SSHGFNEMIEQIMELAETRLKKLDLRRRETVPASELILGMQCGGSDAFSGITANPALGYASDLLLRAGATVMFSEVTEVR
DAIYLLTSRAQDQDVAQALVREMDWYDRYLAKGEADRSANTTPGNKKGGLSNIVEKSLGSIVKSGSSAINGVLGPGERVS
GKGLIFCATPASDFVCGTLQLAAGMNLHVFTTGRGTPYGLAMAPVVKVSTRTELAQRWPDLIDIDAGRIATGRASIEELG
WELFHFYLDVASGKKKTWTEHYRLHNDITLFNPAPIT

Sequences:

>Translated_517_residues
MNLIQHADSPRYIRLHPLDNVVVVVNDQGVPAGTEFDNGLVTVDNVPQSHKVSTVDLAVGEAVIRYGHTIGYALQPIPRG
SWVREDQLRMPSAPALDSLPMSDAVPEKQAPLEGYTFEGYRNADGTVGTRNILGITTTVQCVTGVLDHAVKRIRDELLPK
YPNVDDVVALTHSYGCGVAITATDAYIPIRTVRNLARNPNLGGEALVISLGCEKLQAGQVMHEGDSSVDLSEPWLYRLQD
SSHGFNEMIEQIMELAETRLKKLDLRRRETVPASELILGMQCGGSDAFSGITANPALGYASDLLLRAGATVMFSEVTEVR
DAIYLLTSRAQDQDVAQALVREMDWYDRYLAKGEADRSANTTPGNKKGGLSNIVEKSLGSIVKSGSSAINGVLGPGERVS
GKGLIFCATPASDFVCGTLQLAAGMNLHVFTTGRGTPYGLAMAPVVKVSTRTELAQRWPDLIDIDAGRIATGRASIEELG
WELFHFYLDVASGKKKTWTEHYRLHNDITLFNPAPIT
>Mature_517_residues
MNLIQHADSPRYIRLHPLDNVVVVVNDQGVPAGTEFDNGLVTVDNVPQSHKVSTVDLAVGEAVIRYGHTIGYALQPIPRG
SWVREDQLRMPSAPALDSLPMSDAVPEKQAPLEGYTFEGYRNADGTVGTRNILGITTTVQCVTGVLDHAVKRIRDELLPK
YPNVDDVVALTHSYGCGVAITATDAYIPIRTVRNLARNPNLGGEALVISLGCEKLQAGQVMHEGDSSVDLSEPWLYRLQD
SSHGFNEMIEQIMELAETRLKKLDLRRRETVPASELILGMQCGGSDAFSGITANPALGYASDLLLRAGATVMFSEVTEVR
DAIYLLTSRAQDQDVAQALVREMDWYDRYLAKGEADRSANTTPGNKKGGLSNIVEKSLGSIVKSGSSAINGVLGPGERVS
GKGLIFCATPASDFVCGTLQLAAGMNLHVFTTGRGTPYGLAMAPVVKVSTRTELAQRWPDLIDIDAGRIATGRASIEELG
WELFHFYLDVASGKKKTWTEHYRLHNDITLFNPAPIT

Specific function: D-galactarate metabolism; first step. [C]

COG id: COG2721

COG function: function code G; Altronate dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the uxaA family [H]

Homologues:

Organism=Escherichia coli, GI1789516, Length=516, Percent_Identity=60.4651162790698, Blast_Score=666, Evalue=0.0,
Organism=Escherichia coli, GI1789475, Length=500, Percent_Identity=35.2, Blast_Score=248, Evalue=9e-67,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007392
- InterPro:   IPR017654
- InterPro:   IPR013974 [H]

Pfam domain/function: PF04295 GD_AH_C; PF08666 SAF [H]

EC number: =4.2.1.42 [H]

Molecular weight: Translated: 56070; Mature: 56070

Theoretical pI: Translated: 5.47; Mature: 5.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLIQHADSPRYIRLHPLDNVVVVVNDQGVPAGTEFDNGLVTVDNVPQSHKVSTVDLAVG
CCCCCCCCCCCEEEEECCCCEEEEECCCCCCCCCCCCCCEEEECCCCCCCCEEEEHHHHH
EAVIRYGHTIGYALQPIPRGSWVREDQLRMPSAPALDSLPMSDAVPEKQAPLEGYTFEGY
HHHHHHCCHHCEEECCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCC
RNADGTVGTRNILGITTTVQCVTGVLDHAVKRIRDELLPKYPNVDDVVALTHSYGCGVAI
CCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHEEEEECCCCCEEE
TATDAYIPIRTVRNLARNPNLGGEALVISLGCEKLQAGQVMHEGDSSVDLSEPWLYRLQD
EECCCEEEHHHHHHHHCCCCCCCCEEEEEECCHHHHHCCCEECCCCCCCCCCCCEEEEEC
SSHGFNEMIEQIMELAETRLKKLDLRRRETVPASELILGMQCGGSDAFSGITANPALGYA
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHEEEEEECCCCCCCCCCCCCCCCCHH
SDLLLRAGATVMFSEVTEVRDAIYLLTSRAQDQDVAQALVREMDWYDRYLAKGEADRSAN
HHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
TTPGNKKGGLSNIVEKSLGSIVKSGSSAINGVLGPGERVSGKGLIFCATPASDFVCGTLQ
CCCCCCCCCHHHHHHHHHHHHHHCCCHHHCCCCCCCCCCCCCEEEEEECCCCCHHHHHHH
LAAGMNLHVFTTGRGTPYGLAMAPVVKVSTRTELAQRWPDLIDIDAGRIATGRASIEELG
HHCCCEEEEEECCCCCCCCEECCCEEEEHHHHHHHHHCCCEEECCCCCEECCCHHHHHHH
WELFHFYLDVASGKKKTWTEHYRLHNDITLFNPAPIT
HHHHHHHHHHCCCCCCHHHHHHEEECCEEEECCCCCC
>Mature Secondary Structure
MNLIQHADSPRYIRLHPLDNVVVVVNDQGVPAGTEFDNGLVTVDNVPQSHKVSTVDLAVG
CCCCCCCCCCCEEEEECCCCEEEEECCCCCCCCCCCCCCEEEECCCCCCCCEEEEHHHHH
EAVIRYGHTIGYALQPIPRGSWVREDQLRMPSAPALDSLPMSDAVPEKQAPLEGYTFEGY
HHHHHHCCHHCEEECCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCC
RNADGTVGTRNILGITTTVQCVTGVLDHAVKRIRDELLPKYPNVDDVVALTHSYGCGVAI
CCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHEEEEECCCCCEEE
TATDAYIPIRTVRNLARNPNLGGEALVISLGCEKLQAGQVMHEGDSSVDLSEPWLYRLQD
EECCCEEEHHHHHHHHCCCCCCCCEEEEEECCHHHHHCCCEECCCCCCCCCCCCEEEEEC
SSHGFNEMIEQIMELAETRLKKLDLRRRETVPASELILGMQCGGSDAFSGITANPALGYA
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHEEEEEECCCCCCCCCCCCCCCCCHH
SDLLLRAGATVMFSEVTEVRDAIYLLTSRAQDQDVAQALVREMDWYDRYLAKGEADRSAN
HHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
TTPGNKKGGLSNIVEKSLGSIVKSGSSAINGVLGPGERVSGKGLIFCATPASDFVCGTLQ
CCCCCCCCCHHHHHHHHHHHHHHCCCHHHCCCCCCCCCCCCCEEEEEECCCCCHHHHHHH
LAAGMNLHVFTTGRGTPYGLAMAPVVKVSTRTELAQRWPDLIDIDAGRIATGRASIEELG
HHCCCEEEEEECCCCCCCCEECCCEEEEHHHHHHHHHCCCEEECCCCCEECCCHHHHHHH
WELFHFYLDVASGKKKTWTEHYRLHNDITLFNPAPIT
HHHHHHHHHHCCCCCCHHHHHHEEECCEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7704254; 9384377 [H]