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The map label for this gene is yafV [C]

Identifier: 66047384

GI number: 66047384

Start: 4943793

End: 4944638

Strand: Reverse

Name: yafV [C]

Synonym: Psyr_4157

Alternate gene names: 66047384

Gene position: 4944638-4943793 (Counterclockwise)

Preceding gene: 66047385

Following gene: 66047383

Centisome position: 81.14

GC content: 64.07

Gene sequence:

>846_bases
ATGTCCTTCGCGGTGATTCAGATGGTCAGCCAAAGCGATGTCCTGGCCAATCTGGCCAGCGCTCGGCGTTTGCTGGAGCA
GGCAGCCGACGGTGGCGCGCGGCTGGCGGTGCTGCCCGAAAACTTCGTTGCGATGGGGCGCCGTGACGTCGCCGATATCG
GGCGTGCCGAGGCGCAGGGGCACGGACCGATCCTGCCATGGTTGAAACTGGCCGCTCGCGACCTCAAGTTATGGATTGTT
GCCGGAACCTTGCCACTGCCGCCGGATGAGCGGCCGGATGGCAAGGTGACGGCGTGTTCGCTGTTGATCGATGAGCACGG
TGAGCAGGTGGCCCGTTATGACAAACTGCATCTGTTCGATGTGGATGTCGCTGACAACCGTGGCCGCTATCGTGAATCGG
ATGACTATGCTCATGGGAGCAGGGTGGTGGTAGCCGATACGCCGGTGGGGCGTCTGGGGCTGACCGTCTGTTATGACCTG
CGCTTTCCCGAGCTGTACACCGCGCTGCGTGAAGCCGGTGCTGAACTGATAACCGCGCCGTCAGCCTTCACTGCGGTAAC
GGGGGCTGCGCACTGGGACATTCTGATCCGCGCGCGAGCCATCGAAACCCAGTGTTACGTGCTGGCGGCGGCACAGGGCG
GGGTCCATCCGGGGCCTCGCGAGACTTACGGACACGCGGCCATCGTTGATCCATGGGGCCGCGTACTGGCCGAGCAGGCG
CAGGGTGAAGCGGTGCTGCTGGCCACACGCGACAGTGAAGAACAGGCGTCCATACGGGCGCGGATGCCGGTGTCCAGTCA
CCGGCGCTTTTTTTCGCAGGACGCCATGCGGCCTGCTTCGGAGTGA

Upstream 100 bases:

>100_bases
CTGGAAAGAGCCGAAGATCACCTTCGACAAGCCATTTGAAAAGCCCCAATAGCGCCTCATGGAGTACAGTGGTGGCCATC
TCATTCAGGAGCGGGCTGCC

Downstream 100 bases:

>100_bases
ATATGAGTGATCTGTCCTCAGTCAGCGAACACCTTCTGGCACCCGGCGGCCTGAACCTCGACAGCCTGCAGTCCGTGCTG
GGCGATCTGGCCGGACCAGG

Product: Nitrilase/cyanide hydratase and apolipoprotein N-acyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 281; Mature: 280

Protein sequence:

>281_residues
MSFAVIQMVSQSDVLANLASARRLLEQAADGGARLAVLPENFVAMGRRDVADIGRAEAQGHGPILPWLKLAARDLKLWIV
AGTLPLPPDERPDGKVTACSLLIDEHGEQVARYDKLHLFDVDVADNRGRYRESDDYAHGSRVVVADTPVGRLGLTVCYDL
RFPELYTALREAGAELITAPSAFTAVTGAAHWDILIRARAIETQCYVLAAAQGGVHPGPRETYGHAAIVDPWGRVLAEQA
QGEAVLLATRDSEEQASIRARMPVSSHRRFFSQDAMRPASE

Sequences:

>Translated_281_residues
MSFAVIQMVSQSDVLANLASARRLLEQAADGGARLAVLPENFVAMGRRDVADIGRAEAQGHGPILPWLKLAARDLKLWIV
AGTLPLPPDERPDGKVTACSLLIDEHGEQVARYDKLHLFDVDVADNRGRYRESDDYAHGSRVVVADTPVGRLGLTVCYDL
RFPELYTALREAGAELITAPSAFTAVTGAAHWDILIRARAIETQCYVLAAAQGGVHPGPRETYGHAAIVDPWGRVLAEQA
QGEAVLLATRDSEEQASIRARMPVSSHRRFFSQDAMRPASE
>Mature_280_residues
SFAVIQMVSQSDVLANLASARRLLEQAADGGARLAVLPENFVAMGRRDVADIGRAEAQGHGPILPWLKLAARDLKLWIVA
GTLPLPPDERPDGKVTACSLLIDEHGEQVARYDKLHLFDVDVADNRGRYRESDDYAHGSRVVVADTPVGRLGLTVCYDLR
FPELYTALREAGAELITAPSAFTAVTGAAHWDILIRARAIETQCYVLAAAQGGVHPGPRETYGHAAIVDPWGRVLAEQAQ
GEAVLLATRDSEEQASIRARMPVSSHRRFFSQDAMRPASE

Specific function: Unknown

COG id: COG0388

COG function: function code R; Predicted amidohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

Organism=Homo sapiens, GI297632348, Length=268, Percent_Identity=42.5373134328358, Blast_Score=194, Evalue=6e-50,
Organism=Homo sapiens, GI5031947, Length=268, Percent_Identity=42.1641791044776, Blast_Score=194, Evalue=7e-50,
Organism=Homo sapiens, GI297632350, Length=268, Percent_Identity=42.1641791044776, Blast_Score=194, Evalue=7e-50,
Organism=Homo sapiens, GI9910460, Length=268, Percent_Identity=31.7164179104478, Blast_Score=146, Evalue=2e-35,
Organism=Homo sapiens, GI297632346, Length=196, Percent_Identity=38.265306122449, Blast_Score=118, Evalue=6e-27,
Organism=Escherichia coli, GI1786412, Length=258, Percent_Identity=27.5193798449612, Blast_Score=64, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17556280, Length=267, Percent_Identity=40.4494382022472, Blast_Score=191, Evalue=5e-49,
Organism=Saccharomyces cerevisiae, GI6322335, Length=297, Percent_Identity=34.6801346801347, Blast_Score=162, Evalue=5e-41,
Organism=Saccharomyces cerevisiae, GI6323383, Length=278, Percent_Identity=33.8129496402878, Blast_Score=156, Evalue=3e-39,
Organism=Drosophila melanogaster, GI17933642, Length=274, Percent_Identity=42.3357664233577, Blast_Score=185, Evalue=3e-47,
Organism=Drosophila melanogaster, GI21355835, Length=272, Percent_Identity=30.8823529411765, Blast_Score=142, Evalue=3e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003010
- InterPro:   IPR001110 [H]

Pfam domain/function: PF00795 CN_hydrolase [H]

EC number: 3.5.-.- [C]

Molecular weight: Translated: 30556; Mature: 30425

Theoretical pI: Translated: 5.86; Mature: 5.86

Prosite motif: PS50263 CN_HYDROLASE ; PS01227 UPF0012

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFAVIQMVSQSDVLANLASARRLLEQAADGGARLAVLPENFVAMGRRDVADIGRAEAQG
CCCCEEEHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECHHHHHCCCCCHHHHCCCCCCC
HGPILPWLKLAARDLKLWIVAGTLPLPPDERPDGKVTACSLLIDEHGEQVARYDKLHLFD
CCCCHHHHHHHHCCEEEEEEECCCCCCCCCCCCCCEEEEEEEHHHCCHHHHHHCCEEEEE
VDVADNRGRYRESDDYAHGSRVVVADTPVGRLGLTVCYDLRFPELYTALREAGAELITAP
EEECCCCCCCCCCCCCCCCCEEEEECCCCCCCCEEEEEECCCHHHHHHHHHCCHHEEECC
SAFTAVTGAAHWDILIRARAIETQCYVLAAAQGGVHPGPRETYGHAAIVDPWGRVLAEQA
CHHHHHCCCCEEEEEEEEEEECCEEEEEEEECCCCCCCCCCCCCCEEEECHHHHHHHHHC
QGEAVLLATRDSEEQASIRARMPVSSHRRFFSQDAMRPASE
CCCEEEEEECCCCHHHHEEECCCHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
SFAVIQMVSQSDVLANLASARRLLEQAADGGARLAVLPENFVAMGRRDVADIGRAEAQG
CCCEEEHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECHHHHHCCCCCHHHHCCCCCCC
HGPILPWLKLAARDLKLWIVAGTLPLPPDERPDGKVTACSLLIDEHGEQVARYDKLHLFD
CCCCHHHHHHHHCCEEEEEEECCCCCCCCCCCCCCEEEEEEEHHHCCHHHHHHCCEEEEE
VDVADNRGRYRESDDYAHGSRVVVADTPVGRLGLTVCYDLRFPELYTALREAGAELITAP
EEECCCCCCCCCCCCCCCCCEEEEECCCCCCCCEEEEEECCCHHHHHHHHHCCHHEEECC
SAFTAVTGAAHWDILIRARAIETQCYVLAAAQGGVHPGPRETYGHAAIVDPWGRVLAEQA
CHHHHHCCCCEEEEEEEEEEECCEEEEEEEECCCCCCCCCCCCCCEEEECHHHHHHHHHC
QGEAVLLATRDSEEQASIRARMPVSSHRRFFSQDAMRPASE
CCCEEEEEECCCCHHHHEEECCCHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]