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The map label for this gene is ddl [H]

Identifier: 66047327

GI number: 66047327

Start: 4888521

End: 4889480

Strand: Reverse

Name: ddl [H]

Synonym: Psyr_4100

Alternate gene names: 66047327

Gene position: 4889480-4888521 (Counterclockwise)

Preceding gene: 66047328

Following gene: 66047326

Centisome position: 80.24

GC content: 57.71

Gene sequence:

>960_bases
ATGACAGCCGTTGCCCAGTCGTCCCTGCGCTCGACTCTCGAGCCGAAAAGCTTCGGCCGTGTCGCCGTGCTCTTCGGTGG
CAAGAGTGCCGAGCGTGCGGTCTCGCTGAACTCGGGTAATGCCGTGCTCGGCGCGTTGCTCGAGGCTGGTGTGGATGCGT
TCGGCATCGATGTGGGCGATGACTTCCTGCAGCGACTGGTCAGCGAAAAAATCGACCGTGCCTTTATTGTTCTGCACGGA
CGCGGCGGTGAAGACGGCACCATGCAAGGCCTGCTGGAATGCCTGGAAATCCCTTATACCGGCAGTGGCGTACTGGCTTC
TGCGCTGGCGATGGACAAGCTGCGCACCAAGCAGGTCTGGCAGAGTCTGGGCCTTGCTACACCGGCGCATGCCGTTCTTG
AAAACGAGAGTGATTGTATTTGCGCGGCGACGGAACTGGGCTTGCCTTTGATCGTCAAACCGGCCCATGAAGGTTCAAGT
ATCGGTATGGCAAAGGTGAACAGCGTCGATGAATTGATCGCCGCCTGGAAAGCCGCCAGTACCTACGATTCGCAAGTATT
AGTCGAACAATGGATTCAGGGACCTGAGTTCACTGTGGCGTCTCTGCGTGGCCAGGTGTTGCCTCCCATCGGCCTGGGCA
CTCCTCACAGTTTTTATGATTACGATGCCAAGTACCTTGCGTCCGATACCCAGTATCGGATTCCGTGCGGACTTGGCGAT
GCGCAGGAACAGGAACTCAAACAACTTGCAGCACGTGCTTGCGATGCCATTGGCATTGCAGGGTGGGCGCGCACGGATGT
CATGCAGGACGCACAAGGCAAGTTCTGGTTACTGGAAGTCAATACTGTACCGGGCATGACCGATCACAGTCTGGTGCCTA
TGGCGGCCCGTGCGGCAGGTCTGGATTTTCAACAACTGGTGTTGGCGATTCTGGCCGACAGCGTTCAGGCGAGGGGTTAA

Upstream 100 bases:

>100_bases
TGTGCCAGGGCGCTGGCGACATCGGTGGTCTCGCACCGCAGTTACTCAAGAGCCCGCTGTTCGCCGGTGCCAAAGTGGCC
TCCACCGAAGGGAAGCTGAA

Downstream 100 bases:

>100_bases
GGCATGTACGGCCCGTCGACACGTCCTCAGCCACCTGCATCCGGCCGCAACAAGCCGGTGCCGCGCGGCGCCAGCCGTAT
GGTGGCCAAGGAGCCCTTGT

Product: D-alanine--D-alanine ligase

Products: NA

Alternate protein names: D-Ala-D-Ala ligase B; D-alanylalanine synthetase B [H]

Number of amino acids: Translated: 319; Mature: 318

Protein sequence:

>319_residues
MTAVAQSSLRSTLEPKSFGRVAVLFGGKSAERAVSLNSGNAVLGALLEAGVDAFGIDVGDDFLQRLVSEKIDRAFIVLHG
RGGEDGTMQGLLECLEIPYTGSGVLASALAMDKLRTKQVWQSLGLATPAHAVLENESDCICAATELGLPLIVKPAHEGSS
IGMAKVNSVDELIAAWKAASTYDSQVLVEQWIQGPEFTVASLRGQVLPPIGLGTPHSFYDYDAKYLASDTQYRIPCGLGD
AQEQELKQLAARACDAIGIAGWARTDVMQDAQGKFWLLEVNTVPGMTDHSLVPMAARAAGLDFQQLVLAILADSVQARG

Sequences:

>Translated_319_residues
MTAVAQSSLRSTLEPKSFGRVAVLFGGKSAERAVSLNSGNAVLGALLEAGVDAFGIDVGDDFLQRLVSEKIDRAFIVLHG
RGGEDGTMQGLLECLEIPYTGSGVLASALAMDKLRTKQVWQSLGLATPAHAVLENESDCICAATELGLPLIVKPAHEGSS
IGMAKVNSVDELIAAWKAASTYDSQVLVEQWIQGPEFTVASLRGQVLPPIGLGTPHSFYDYDAKYLASDTQYRIPCGLGD
AQEQELKQLAARACDAIGIAGWARTDVMQDAQGKFWLLEVNTVPGMTDHSLVPMAARAAGLDFQQLVLAILADSVQARG
>Mature_318_residues
TAVAQSSLRSTLEPKSFGRVAVLFGGKSAERAVSLNSGNAVLGALLEAGVDAFGIDVGDDFLQRLVSEKIDRAFIVLHGR
GGEDGTMQGLLECLEIPYTGSGVLASALAMDKLRTKQVWQSLGLATPAHAVLENESDCICAATELGLPLIVKPAHEGSSI
GMAKVNSVDELIAAWKAASTYDSQVLVEQWIQGPEFTVASLRGQVLPPIGLGTPHSFYDYDAKYLASDTQYRIPCGLGDA
QEQELKQLAARACDAIGIAGWARTDVMQDAQGKFWLLEVNTVPGMTDHSLVPMAARAAGLDFQQLVLAILADSVQARG

Specific function: Cell wall formation [H]

COG id: COG1181

COG function: function code M; D-alanine-D-alanine ligase and related ATP-grasp enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1786280, Length=299, Percent_Identity=52.1739130434783, Blast_Score=301, Evalue=4e-83,
Organism=Escherichia coli, GI1786579, Length=344, Percent_Identity=32.2674418604651, Blast_Score=151, Evalue=4e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR000291
- InterPro:   IPR005905
- InterPro:   IPR011095
- InterPro:   IPR011127
- InterPro:   IPR013817
- InterPro:   IPR016185 [H]

Pfam domain/function: PF07478 Dala_Dala_lig_C; PF01820 Dala_Dala_lig_N [H]

EC number: =6.3.2.4 [H]

Molecular weight: Translated: 33800; Mature: 33669

Theoretical pI: Translated: 4.56; Mature: 4.56

Prosite motif: PS50975 ATP_GRASP ; PS00843 DALA_DALA_LIGASE_1 ; PS00844 DALA_DALA_LIGASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAVAQSSLRSTLEPKSFGRVAVLFGGKSAERAVSLNSGNAVLGALLEAGVDAFGIDVGD
CCCHHHHHHHHHCCCCCCCCEEEEECCCCCCCEEEECCCCHHHHHHHHCCCCEEECCCCH
DFLQRLVSEKIDRAFIVLHGRGGEDGTMQGLLECLEIPYTGSGVLASALAMDKLRTKQVW
HHHHHHHHHHCCEEEEEEECCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHH
QSLGLATPAHAVLENESDCICAATELGLPLIVKPAHEGSSIGMAKVNSVDELIAAWKAAS
HHHCCCCCHHHHHCCCCCCEEEHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHCC
TYDSQVLVEQWIQGPEFTVASLRGQVLPPIGLGTPHSFYDYDAKYLASDTQYRIPCGLGD
CCCHHHHHHHHCCCCCEEHHHHCCCCCCCCCCCCCCCCCCCCHHHHCCCCCEEECCCCCC
AQEQELKQLAARACDAIGIAGWARTDVMQDAQGKFWLLEVNTVPGMTDHSLVPMAARAAG
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCEEEEEECCCCCCCCCCHHHHHHHHHC
LDFQQLVLAILADSVQARG
CCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TAVAQSSLRSTLEPKSFGRVAVLFGGKSAERAVSLNSGNAVLGALLEAGVDAFGIDVGD
CCHHHHHHHHHCCCCCCCCEEEEECCCCCCCEEEECCCCHHHHHHHHCCCCEEECCCCH
DFLQRLVSEKIDRAFIVLHGRGGEDGTMQGLLECLEIPYTGSGVLASALAMDKLRTKQVW
HHHHHHHHHHCCEEEEEEECCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHH
QSLGLATPAHAVLENESDCICAATELGLPLIVKPAHEGSSIGMAKVNSVDELIAAWKAAS
HHHCCCCCHHHHHCCCCCCEEEHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHCC
TYDSQVLVEQWIQGPEFTVASLRGQVLPPIGLGTPHSFYDYDAKYLASDTQYRIPCGLGD
CCCHHHHHHHHCCCCCEEHHHHCCCCCCCCCCCCCCCCCCCCHHHHCCCCCEEECCCCCC
AQEQELKQLAARACDAIGIAGWARTDVMQDAQGKFWLLEVNTVPGMTDHSLVPMAARAAG
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCEEEEEECCCCCCCCCCHHHHHHHHHC
LDFQQLVLAILADSVQARG
CCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12928499 [H]