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The map label for this gene is nylB' [H]

Identifier: 66047274

GI number: 66047274

Start: 4830485

End: 4831591

Strand: Reverse

Name: nylB' [H]

Synonym: Psyr_4047

Alternate gene names: 66047274

Gene position: 4831591-4830485 (Counterclockwise)

Preceding gene: 66047277

Following gene: 66047273

Centisome position: 79.29

GC content: 61.7

Gene sequence:

>1107_bases
ATGCCGCGTGGATTTGTCCGCCTGTGTCTGGTTCTGATCCTGGGTTTCAGTGCCCAGGGCGTCGTTGCTCAAACATGGCC
GACAGAGCAATGGCCTTCTCGGTCTGTGCCGCGATCTGCGGCCATCGAAGAGCTTGAGGCCTACGCTTTCCCGCCTCGCG
ACGACGCCACGCGCAAGGGCGTACGCACCGATGCGCTGCTGGTGATTCGTGATGGCGACGTTGTGTACGAGCGCTATGCC
GGTGTGACCACCGCGCAAACGCCGCACCTGACCTGGTCGATCAGCAAAAGCATCATGGCGACGGTGTTGGGGGTGGCGTT
CGCAGAAGGGCGCTTTCAACTGAATGACCCGGTGGCGAAGTTCTACACGCCGTTCAAGGCGCACCCCGATATCGCCATCA
AAGACCTGATGCACTGGGCTTCGGGTCTGGATTGGCAGGAAGACTACGAATACGCTCCGTTCAACTCCTCGGTGGTTGCC
ATGCTCTACACCCGCGGGCGTGACGACATGGCGCGTTTCACTGCCGATCACAGCGCTGCCCAGCACCCTGGCGAGACTTA
CCTTTATTCCAGCGGCGACAGCACCGTGCTGGCGGCGGCCTTGAAAAAGATGGTCGGCGATGAGGCTTATCCGGGTTATC
CATGGACCGCGCTGTTCGACCCGCTCGGCATCCGCAGTGCGGTCTGGGAAACCGATGGCGCGGGTACATTTGTAGGTTCG
TCCTATGCCTACATGACCGCCCGCGATCTGGCCCGCATCGGCCTGCTGATGCAGCGCGGCGGGCAATGGCAGGATCGGCA
GTTGCTCAACAAGCAGTGGATGGACTTCGTGCTGACGCCGTTTGCCGGTGCAGGCGTGGATGCCAGCGTTGAAGTGCCCG
CAGGTCAATGGTGGCTTAACCGTACTGGCGATGGCGGCAAAGGACCGTGGCCGGACGCTCCCCCCGACACCTTCGCGGCA
CTGGGGCATTGGGGGCAGGCGCTGTATGTGATTCCCGATCAGAAGCTGGTGATCGTGCGCTATGCCGATGACCGCGACAG
TACCTACAACCACAATGAGATGCTCAAGCGCGTACAGGCCGCGTTCGGCAAGGAGGGCGGTCAATGA

Upstream 100 bases:

>100_bases
GAAGCGCTGTAGCCATTTCCTCTGCGTCTGCTCTTGGGTTGATAGCTGACTGTCATGAAACGCTGATACGGTGTGCGGAT
CATTCTGTAGAGGCCTTGTC

Downstream 100 bases:

>100_bases
GCTCGCGTCGAATGTTCATCCTGCGCCGTCCCTTCAGCAGCCTGCTGTTGCTGATCGTCGCCGCACTTGCCGTACTCGCC
TGGCAGTACCGTGTCAACCT

Product: Beta-lactamase

Products: NA

Alternate protein names: Nylon oligomers-degrading enzyme EII' [H]

Number of amino acids: Translated: 368; Mature: 367

Protein sequence:

>368_residues
MPRGFVRLCLVLILGFSAQGVVAQTWPTEQWPSRSVPRSAAIEELEAYAFPPRDDATRKGVRTDALLVIRDGDVVYERYA
GVTTAQTPHLTWSISKSIMATVLGVAFAEGRFQLNDPVAKFYTPFKAHPDIAIKDLMHWASGLDWQEDYEYAPFNSSVVA
MLYTRGRDDMARFTADHSAAQHPGETYLYSSGDSTVLAAALKKMVGDEAYPGYPWTALFDPLGIRSAVWETDGAGTFVGS
SYAYMTARDLARIGLLMQRGGQWQDRQLLNKQWMDFVLTPFAGAGVDASVEVPAGQWWLNRTGDGGKGPWPDAPPDTFAA
LGHWGQALYVIPDQKLVIVRYADDRDSTYNHNEMLKRVQAAFGKEGGQ

Sequences:

>Translated_368_residues
MPRGFVRLCLVLILGFSAQGVVAQTWPTEQWPSRSVPRSAAIEELEAYAFPPRDDATRKGVRTDALLVIRDGDVVYERYA
GVTTAQTPHLTWSISKSIMATVLGVAFAEGRFQLNDPVAKFYTPFKAHPDIAIKDLMHWASGLDWQEDYEYAPFNSSVVA
MLYTRGRDDMARFTADHSAAQHPGETYLYSSGDSTVLAAALKKMVGDEAYPGYPWTALFDPLGIRSAVWETDGAGTFVGS
SYAYMTARDLARIGLLMQRGGQWQDRQLLNKQWMDFVLTPFAGAGVDASVEVPAGQWWLNRTGDGGKGPWPDAPPDTFAA
LGHWGQALYVIPDQKLVIVRYADDRDSTYNHNEMLKRVQAAFGKEGGQ
>Mature_367_residues
PRGFVRLCLVLILGFSAQGVVAQTWPTEQWPSRSVPRSAAIEELEAYAFPPRDDATRKGVRTDALLVIRDGDVVYERYAG
VTTAQTPHLTWSISKSIMATVLGVAFAEGRFQLNDPVAKFYTPFKAHPDIAIKDLMHWASGLDWQEDYEYAPFNSSVVAM
LYTRGRDDMARFTADHSAAQHPGETYLYSSGDSTVLAAALKKMVGDEAYPGYPWTALFDPLGIRSAVWETDGAGTFVGSS
YAYMTARDLARIGLLMQRGGQWQDRQLLNKQWMDFVLTPFAGAGVDASVEVPAGQWWLNRTGDGGKGPWPDAPPDTFAAL
GHWGQALYVIPDQKLVIVRYADDRDSTYNHNEMLKRVQAAFGKEGGQ

Specific function: Unknown

COG id: COG1680

COG function: function code V; Beta-lactamase class C and other penicillin binding proteins

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001466 [H]

Pfam domain/function: PF00144 Beta-lactamase [H]

EC number: =3.5.1.46 [H]

Molecular weight: Translated: 40728; Mature: 40597

Theoretical pI: Translated: 5.41; Mature: 5.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRGFVRLCLVLILGFSAQGVVAQTWPTEQWPSRSVPRSAAIEELEAYAFPPRDDATRKG
CCHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCHHHCC
VRTDALLVIRDGDVVYERYAGVTTAQTPHLTWSISKSIMATVLGVAFAEGRFQLNDPVAK
CCCCEEEEEECCCEEEHHHCCCCCCCCCCEEEHHHHHHHHHHHHHHHHCCEEEECCCHHH
FYTPFKAHPDIAIKDLMHWASGLDWQEDYEYAPFNSSVVAMLYTRGRDDMARFTADHSAA
HCCCCCCCCCHHHHHHHHHHCCCCCHHCCCCCCCCCCEEEEEECCCCHHHHHHHHCCHHH
QHPGETYLYSSGDSTVLAAALKKMVGDEAYPGYPWTALFDPLGIRSAVWETDGAGTFVGS
CCCCCEEEEECCCCHHHHHHHHHHHCCCCCCCCCHHHHHCCCCCHHHHCCCCCCCEEECC
SYAYMTARDLARIGLLMQRGGQWQDRQLLNKQWMDFVLTPFAGAGVDASVEVPAGQWWLN
CCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCEEECCCCCEEEE
RTGDGGKGPWPDAPPDTFAALGHWGQALYVIPDQKLVIVRYADDRDSTYNHNEMLKRVQA
CCCCCCCCCCCCCCHHHHHHHHCCCCEEEEECCCEEEEEEEECCCCCCCCHHHHHHHHHH
AFGKEGGQ
HHCCCCCC
>Mature Secondary Structure 
PRGFVRLCLVLILGFSAQGVVAQTWPTEQWPSRSVPRSAAIEELEAYAFPPRDDATRKG
CHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCHHHCC
VRTDALLVIRDGDVVYERYAGVTTAQTPHLTWSISKSIMATVLGVAFAEGRFQLNDPVAK
CCCCEEEEEECCCEEEHHHCCCCCCCCCCEEEHHHHHHHHHHHHHHHHCCEEEECCCHHH
FYTPFKAHPDIAIKDLMHWASGLDWQEDYEYAPFNSSVVAMLYTRGRDDMARFTADHSAA
HCCCCCCCCCHHHHHHHHHHCCCCCHHCCCCCCCCCCEEEEEECCCCHHHHHHHHCCHHH
QHPGETYLYSSGDSTVLAAALKKMVGDEAYPGYPWTALFDPLGIRSAVWETDGAGTFVGS
CCCCCEEEEECCCCHHHHHHHHHHHCCCCCCCCCHHHHHCCCCCHHHHCCCCCCCEEECC
SYAYMTARDLARIGLLMQRGGQWQDRQLLNKQWMDFVLTPFAGAGVDASVEVPAGQWWLN
CCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCEEECCCCCEEEE
RTGDGGKGPWPDAPPDTFAALGHWGQALYVIPDQKLVIVRYADDRDSTYNHNEMLKRVQA
CCCCCCCCCCCCCCHHHHHHHHCCCCEEEEECCCEEEEEEEECCCCCCCCHHHHHHHHHH
AFGKEGGQ
HHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6646204; 6389532; 1879421 [H]