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The map label for this gene is purU [H]

Identifier: 66047245

GI number: 66047245

Start: 4785483

End: 4786334

Strand: Reverse

Name: purU [H]

Synonym: Psyr_4018

Alternate gene names: 66047245

Gene position: 4786334-4785483 (Counterclockwise)

Preceding gene: 66047247

Following gene: 66047244

Centisome position: 78.55

GC content: 58.45

Gene sequence:

>852_bases
ATGCGCACTTTTCGTCTGGTGATTGCTTGCCCGGACCGTGTAGGCATCGTTGCCAAAGTCAGTAACTTTCTGGCGTCCCA
TAACGGCTGGATCACCGAAGCGAGTCATCACTCGGACAATCTCAGCGGCTGGTTTTTCATGCGTCACGAGATCCGTGCCG
ACACCCTGCCTTTTGATCTGGACGGGTTCCGTGAAGCCTTCACGCCGATTGCCGAAGAATTTTCGATGGACTGGCGCATT
ACCGACTCGGCGCAGAAGAAGCGCGTCGTGCTCATGGCCAGTCGCGAATCACACTGTCTGGCCGACCTGCTGCACCGCTG
GCACAGTGACGAACTCGACTGCGACATCGCCTGTGTGATTTCAAACCACCAGGACCTGCGCAGCATGGTCGAGTGGCATG
ACATCCCTTACTACCATGTTCCGGTTGACCCGAAAGACAAGGAGCCGGCATTCGCCGAGGTGTCGCGTCTGGTCGGTCAT
CATCAGGCCGACGTCGTGGTTCTGGCGCGCTACATGCAGATTCTGCCGCCACAGTTGTGCCGTGAATATGCCCATCAGGT
CATCAATATCCACCACAGCTTCCTGCCATCGTTCGTGGGTGCCAAGCCGTACCATCAGGCCTCGTTGCGCGGCGTCAAGC
TGATCGGCGCGACCTGTCACTATGTGACCGAAGAGCTCGATGCCGGTCCGATCATCGAGCAGGACGTCGTGCGCGTCAGC
CACCGTGACAGCATCGAAAACATGGTGCGTTTCGGCCGCGACGTGGAGAAGATGGTGTTGGCCCGCGGCTTGCGCGCTCA
TCTGGAAGACCGTGTGCTGGTTCACGACAACAAGACAGTCGTCTTCGGCTGA

Upstream 100 bases:

>100_bases
GTGTGCTTTTTCGGCCTCTGCATTCAGGCAACAGAGATACTGCAGTTTTGCTGCGCAGCCGCTAGAATAGCGGCTTTGCG
AAGTTCTGGAGTTTCCCCCA

Downstream 100 bases:

>100_bases
TCTATCTTTTCGGGAGCGCGTCTGTCGCGCTTCCGACTCAGCTGTCGGCAATGGTGTAACGCGCAAGGTGACAGCCAATG
ACCGATCCTCTCGACAAAGC

Product: formyltetrahydrofolate deformylase

Products: NA

Alternate protein names: Formyl-FH(4) hydrolase [H]

Number of amino acids: Translated: 283; Mature: 283

Protein sequence:

>283_residues
MRTFRLVIACPDRVGIVAKVSNFLASHNGWITEASHHSDNLSGWFFMRHEIRADTLPFDLDGFREAFTPIAEEFSMDWRI
TDSAQKKRVVLMASRESHCLADLLHRWHSDELDCDIACVISNHQDLRSMVEWHDIPYYHVPVDPKDKEPAFAEVSRLVGH
HQADVVVLARYMQILPPQLCREYAHQVINIHHSFLPSFVGAKPYHQASLRGVKLIGATCHYVTEELDAGPIIEQDVVRVS
HRDSIENMVRFGRDVEKMVLARGLRAHLEDRVLVHDNKTVVFG

Sequences:

>Translated_283_residues
MRTFRLVIACPDRVGIVAKVSNFLASHNGWITEASHHSDNLSGWFFMRHEIRADTLPFDLDGFREAFTPIAEEFSMDWRI
TDSAQKKRVVLMASRESHCLADLLHRWHSDELDCDIACVISNHQDLRSMVEWHDIPYYHVPVDPKDKEPAFAEVSRLVGH
HQADVVVLARYMQILPPQLCREYAHQVINIHHSFLPSFVGAKPYHQASLRGVKLIGATCHYVTEELDAGPIIEQDVVRVS
HRDSIENMVRFGRDVEKMVLARGLRAHLEDRVLVHDNKTVVFG
>Mature_283_residues
MRTFRLVIACPDRVGIVAKVSNFLASHNGWITEASHHSDNLSGWFFMRHEIRADTLPFDLDGFREAFTPIAEEFSMDWRI
TDSAQKKRVVLMASRESHCLADLLHRWHSDELDCDIACVISNHQDLRSMVEWHDIPYYHVPVDPKDKEPAFAEVSRLVGH
HQADVVVLARYMQILPPQLCREYAHQVINIHHSFLPSFVGAKPYHQASLRGVKLIGATCHYVTEELDAGPIIEQDVVRVS
HRDSIENMVRFGRDVEKMVLARGLRAHLEDRVLVHDNKTVVFG

Specific function: Produces Formate From Formyl-Tetrahydrofolate. Provides The Major Source Of Formate For The Purt-Dependent Synthesis Of 5'-Phosphoribosyl-N-Formylglycinamide (Fgar) During Aerobic Growth. Has A Role In Regulating The One-Carbon Pool. [C]

COG id: COG0788

COG function: function code F; Formyltetrahydrofolate hydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ACT domain [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=85, Percent_Identity=40, Blast_Score=72, Evalue=7e-13,
Organism=Homo sapiens, GI209869995, Length=85, Percent_Identity=40, Blast_Score=72, Evalue=7e-13,
Organism=Homo sapiens, GI209869993, Length=85, Percent_Identity=40, Blast_Score=72, Evalue=7e-13,
Organism=Escherichia coli, GI1787483, Length=277, Percent_Identity=42.2382671480144, Blast_Score=225, Evalue=2e-60,
Organism=Escherichia coli, GI1788846, Length=193, Percent_Identity=26.9430051813472, Blast_Score=72, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI17567511, Length=188, Percent_Identity=31.3829787234043, Blast_Score=89, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24582400, Length=187, Percent_Identity=27.807486631016, Blast_Score=68, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR002376
- InterPro:   IPR004810 [H]

Pfam domain/function: PF01842 ACT; PF00551 Formyl_trans_N [H]

EC number: =3.5.1.10 [H]

Molecular weight: Translated: 32471; Mature: 32471

Theoretical pI: Translated: 6.67; Mature: 6.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRTFRLVIACPDRVGIVAKVSNFLASHNGWITEASHHSDNLSGWFFMRHEIRADTLPFDL
CCEEEEEEECCCCHHHHHHHHHHHHHCCCCEEECCCCCCCCCEEEEEEEECCCCCCCCCH
DGFREAFTPIAEEFSMDWRITDSAQKKRVVLMASRESHCLADLLHRWHSDELDCDIACVI
HHHHHHHHHHHHHHCCCEEECCCCCCCEEEEEECCCCHHHHHHHHHHCCCCCCCEEEEEE
SNHQDLRSMVEWHDIPYYHVPVDPKDKEPAFAEVSRLVGHHQADVVVLARYMQILPPQLC
CCCHHHHHHHHHHCCCEEECCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHH
REYAHQVINIHHSFLPSFVGAKPYHQASLRGVKLIGATCHYVTEELDAGPIIEQDVVRVS
HHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCEEHHHHHHHHHHCCCCCCCHHHHHHHH
HRDSIENMVRFGRDVEKMVLARGLRAHLEDRVLVHDNKTVVFG
HHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCEEEECCCEEEEC
>Mature Secondary Structure
MRTFRLVIACPDRVGIVAKVSNFLASHNGWITEASHHSDNLSGWFFMRHEIRADTLPFDL
CCEEEEEEECCCCHHHHHHHHHHHHHCCCCEEECCCCCCCCCEEEEEEEECCCCCCCCCH
DGFREAFTPIAEEFSMDWRITDSAQKKRVVLMASRESHCLADLLHRWHSDELDCDIACVI
HHHHHHHHHHHHHHCCCEEECCCCCCCEEEEEECCCCHHHHHHHHHHCCCCCCCEEEEEE
SNHQDLRSMVEWHDIPYYHVPVDPKDKEPAFAEVSRLVGHHQADVVVLARYMQILPPQLC
CCCHHHHHHHHHHCCCEEECCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHH
REYAHQVINIHHSFLPSFVGAKPYHQASLRGVKLIGATCHYVTEELDAGPIIEQDVVRVS
HHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCEEHHHHHHHHHHCCCCCCCHHHHHHHH
HRDSIENMVRFGRDVEKMVLARGLRAHLEDRVLVHDNKTVVFG
HHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCEEEECCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]