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The map label for this gene is kgtP [H]

Identifier: 66047237

GI number: 66047237

Start: 4776902

End: 4778203

Strand: Reverse

Name: kgtP [H]

Synonym: Psyr_4010

Alternate gene names: 66047237

Gene position: 4778203-4776902 (Counterclockwise)

Preceding gene: 66047238

Following gene: 66047233

Centisome position: 78.41

GC content: 58.99

Gene sequence:

>1302_bases
ATGACTACAACATCAGAAGCACCGCATTACACCGGCGAGGAGCGAAGCAAAAGAATTTTCGCGATTGTCGGCGCCTCATC
CGGCAACCTGGTCGAATGGTTCGACTTCTATATCTACGCCTTCTGCGCGATTTATTTCGCACCGGCTTTCTTCCCTTCCG
ATGATCCGACCGTTCAGTTGCTCAACACCGCGGGTGTGTTTGCCGCTGGCTTTCTGATGCGTCCGATTGGCGGCTGGCTG
TTTGGTCGTGTCGCGGACAAACATGGGCGCAAGAACTCCATGCTGATTTCGGTCACCATGATGTGTGCCGGATCACTGAT
CATCGCCTGCCTGCCGACCTATGCGTCGATTGGTGCCTGGGCGCCGGCCTTGCTGCTGATGGCGCGCCTGCTGCAAGGTC
TGTCGGTAGGTGGCGAGTACGGCACCACGGCCACCTACATGAGTGAAGTGGCGCTACGTGGCCAGCGCGGCTTTTATGCT
TCGTTCCAGTACGTGACCCTGATCGGCGGGCAACTGCTGGCCGTGTTGACGGTGGTCATCCTCCAGCAATTCCTGACCAC
CGAAGAGTTGCGTGACTACGGCTGGCGCATCCCCTTCGTGATCGGTGCTGGCGCTGCGGTCATTGCCTTGCTGCTGCGTC
GCACCCTCAATGAAACCACCACGGCCGAAAGCCGGCAGGATAAGGACGCGGGCAGTATCGCGGCACTGTTCAAGCATCAT
GCGGCTGCCTTCATCACCGTGCTGGGTTACACCGCCGGTGGCTCGCTGATTTTCTACACCTTCACGACCTACATGCAGAA
ATACCTGGTCAACACCGGGGGCATGGAAGCCAAGACCGCCAGCTACATCATGACCGGCGCGCTGTTTCTATACATGTGCA
TGCAGCCGTTCTTCGGCATGCTCGCGGACCGCATCGGCCGTCGCAATTCGATGCTCCTGTTCGGCGCGCTGGGTACGCTG
TGTACAGTGCCGATCCTGATGACGCTCAAGACCACCACCAACCCGTTCATTGCCTTCGTGCTGATTACCCTGGCGCTGGC
GATCGTCAGCTTCTACACCTCGATCAGCGGGCTGGTCAAAGCCGAGATGTTTCCGCCTCAGGTTCGTGCGCTGGGCGTGG
GGCTGGCGTATGCGGTGGCCAACGCGGTGTTCGGCGGCTCGGCTGAGTGGGTTGCCCTGAAGCTCAAGAGTGCCGGAATG
GAAAACAGCTTTTACTGGTACGTGACGGCGATGATGGCGGTAGCGTTCCTGTTCAGTCTGCGTTTGCCCAAAGAGCCCAA
GTACCTGCATCAGGATCACTGA

Upstream 100 bases:

>100_bases
TACCGCTATTCCTGTCGGCTGAATTGGCCTGATTGATTGTGCTCGATTAAAGACGGTGTTACAGATTGTTTCTTATGGAC
AGTTCTGCTTGGGGAAAATA

Downstream 100 bases:

>100_bases
TCGCAGGTGTGTTTCGGCCATAAAAAAACCCACCTGATCAGGTGGGTTTTTTATGCACTGCAATCAGAACAACTGAACCG
AAGGCTTCTCCTTGGAGATG

Product: citrate-proton symport

Products: Proton [Cytoplasm]; 2-oxoglutarate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 433; Mature: 432

Protein sequence:

>433_residues
MTTTSEAPHYTGEERSKRIFAIVGASSGNLVEWFDFYIYAFCAIYFAPAFFPSDDPTVQLLNTAGVFAAGFLMRPIGGWL
FGRVADKHGRKNSMLISVTMMCAGSLIIACLPTYASIGAWAPALLLMARLLQGLSVGGEYGTTATYMSEVALRGQRGFYA
SFQYVTLIGGQLLAVLTVVILQQFLTTEELRDYGWRIPFVIGAGAAVIALLLRRTLNETTTAESRQDKDAGSIAALFKHH
AAAFITVLGYTAGGSLIFYTFTTYMQKYLVNTGGMEAKTASYIMTGALFLYMCMQPFFGMLADRIGRRNSMLLFGALGTL
CTVPILMTLKTTTNPFIAFVLITLALAIVSFYTSISGLVKAEMFPPQVRALGVGLAYAVANAVFGGSAEWVALKLKSAGM
ENSFYWYVTAMMAVAFLFSLRLPKEPKYLHQDH

Sequences:

>Translated_433_residues
MTTTSEAPHYTGEERSKRIFAIVGASSGNLVEWFDFYIYAFCAIYFAPAFFPSDDPTVQLLNTAGVFAAGFLMRPIGGWL
FGRVADKHGRKNSMLISVTMMCAGSLIIACLPTYASIGAWAPALLLMARLLQGLSVGGEYGTTATYMSEVALRGQRGFYA
SFQYVTLIGGQLLAVLTVVILQQFLTTEELRDYGWRIPFVIGAGAAVIALLLRRTLNETTTAESRQDKDAGSIAALFKHH
AAAFITVLGYTAGGSLIFYTFTTYMQKYLVNTGGMEAKTASYIMTGALFLYMCMQPFFGMLADRIGRRNSMLLFGALGTL
CTVPILMTLKTTTNPFIAFVLITLALAIVSFYTSISGLVKAEMFPPQVRALGVGLAYAVANAVFGGSAEWVALKLKSAGM
ENSFYWYVTAMMAVAFLFSLRLPKEPKYLHQDH
>Mature_432_residues
TTTSEAPHYTGEERSKRIFAIVGASSGNLVEWFDFYIYAFCAIYFAPAFFPSDDPTVQLLNTAGVFAAGFLMRPIGGWLF
GRVADKHGRKNSMLISVTMMCAGSLIIACLPTYASIGAWAPALLLMARLLQGLSVGGEYGTTATYMSEVALRGQRGFYAS
FQYVTLIGGQLLAVLTVVILQQFLTTEELRDYGWRIPFVIGAGAAVIALLLRRTLNETTTAESRQDKDAGSIAALFKHHA
AAFITVLGYTAGGSLIFYTFTTYMQKYLVNTGGMEAKTASYIMTGALFLYMCMQPFFGMLADRIGRRNSMLLFGALGTLC
TVPILMTLKTTTNPFIAFVLITLALAIVSFYTSISGLVKAEMFPPQVRALGVGLAYAVANAVFGGSAEWVALKLKSAGME
NSFYWYVTAMMAVAFLFSLRLPKEPKYLHQDH

Specific function: Uptake of alpha-ketoglutarate across the boundary membrane with the concomitant import of a cation (symport system) [H]

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1788942, Length=426, Percent_Identity=67.3708920187793, Blast_Score=548, Evalue=1e-157,
Organism=Escherichia coli, GI1790550, Length=408, Percent_Identity=31.1274509803922, Blast_Score=200, Evalue=1e-52,
Organism=Escherichia coli, GI1788292, Length=392, Percent_Identity=32.3979591836735, Blast_Score=182, Evalue=4e-47,
Organism=Escherichia coli, GI1789941, Length=444, Percent_Identity=31.0810810810811, Blast_Score=159, Evalue=4e-40,
Organism=Escherichia coli, GI87082231, Length=326, Percent_Identity=26.9938650306748, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI87082404, Length=315, Percent_Identity=25.7142857142857, Blast_Score=75, Evalue=6e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 47237; Mature: 47106

Theoretical pI: Translated: 9.11; Mature: 9.11

Prosite motif: PS50850 MFS ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTTSEAPHYTGEERSKRIFAIVGASSGNLVEWFDFYIYAFCAIYFAPAFFPSDDPTVQL
CCCCCCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEE
LNTAGVFAAGFLMRPIGGWLFGRVADKHGRKNSMLISVTMMCAGSLIIACLPTYASIGAW
EHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHH
APALLLMARLLQGLSVGGEYGTTATYMSEVALRGQRGFYASFQYVTLIGGQLLAVLTVVI
HHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHH
LQQFLTTEELRDYGWRIPFVIGAGAAVIALLLRRTLNETTTAESRQDKDAGSIAALFKHH
HHHHHHHHHHHHCCCCCCEEECCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHH
AAAFITVLGYTAGGSLIFYTFTTYMQKYLVNTGGMEAKTASYIMTGALFLYMCMQPFFGM
HHHHHHHHHCCCCCCEEEHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
LADRIGRRNSMLLFGALGTLCTVPILMTLKTTTNPFIAFVLITLALAIVSFYTSISGLVK
HHHHHCCCCCEEEHHHHHHHHHHHHHHHEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
AEMFPPQVRALGVGLAYAVANAVFGGSAEWVALKLKSAGMENSFYWYVTAMMAVAFLFSL
HHCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHH
RLPKEPKYLHQDH
CCCCCCCCCCCCC
>Mature Secondary Structure 
TTTSEAPHYTGEERSKRIFAIVGASSGNLVEWFDFYIYAFCAIYFAPAFFPSDDPTVQL
CCCCCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEE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CCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; 2-oxoglutarate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + 2-oxoglutarate [Periplasm] = Proton [Cytoplasm] + 2-oxoglutarate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2118499; 9205837; 9278503; 1709493; 2190187; 2053984; 8419306 [H]