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The map label for this gene is mepA [H]

Identifier: 66047234

GI number: 66047234

Start: 4771083

End: 4772243

Strand: Direct

Name: mepA [H]

Synonym: Psyr_4007

Alternate gene names: 66047234

Gene position: 4771083-4772243 (Clockwise)

Preceding gene: 66047231

Following gene: 66047235

Centisome position: 78.3

GC content: 61.15

Gene sequence:

>1161_bases
ATGCAATTCAAGCCAGCTGTTACCGTTCTGGTCACTGCCATCGCCCTGGCAACCCTGCTCAGTGGCTGTAGCAAGAAGGA
ACAGGCCGCCCCGCCTGCGCAAACCCCTCAGGTAGGCGTCGTTACCATCAAAGCACAGCCCTATACCCTGACCACCGAGC
TGCCGGGCCGGACCACCGCCTTCCGCGTCGCCGAGGTTCGCCCTCAGGTCAACGGCATCATTCTCAAGCGTCTGTTCACC
GAAGGTGGCGATGTCAAAGCCGGTCAGCAGCTCTACCAGATCGACCCGGCTGTCTATGAAGCCAACGCCAACAGCGCCAA
GGCCACCCTGCAGTCCGCCAAGTCGATGTCCGATCGCTACAAGCAACTGGTCAGCGAGCAGGCAGTGAGCCGTCAGGAGT
ACGACACGGCGCTGGCATCGACCCAGGAAGCTCAGGCAGCCCTGCAGACCGCCCAGATCAACCTGCGCTACACCAAGGTG
CTGGCACCTATTTCCGGTCGTATCGGTCGCTCCGCGGTGACGGAAGGCGCACTGGTGAGCAGCGCACAGACCGATGCGAT
GGCAACCATCCAGCAACTCGACCCGATCTATGTCGACGTGGTGCAGTCATCTGCCGACATGCTCAAGCTGCGCAGCGACC
TGGATAGCGGCAAACTGCAGAAAGCCGGCGACAACGCGGCCAAGGTCAAGCTGACCCTGGAAGACAACAGCGTCTACGCC
CAGGAAGGCAAACTGGAGTTCTCCGAAGTATCGGTTGACCAGACCACCGGCTCCGTGACCCTGCGTGCCGTGTTCCCGAA
CCCTGAACACCGCCTGCTGCCGGGCATGTTCGTTCATGCGCAGTTGCAGGCTGGCGTCAATTCGAAAGCCATCCTCGCAC
CGCAGCAAGGCGTAACCCGCGACCTGAAAGGCACGCCGACCGCCATGGTCGTCAATCAGGACAACAAGGTTGAAATGCGT
ACGCTGGTGGCCAATCGCACCGTGGGCAGCGACTGGCTGATCGAAGACGGCCTGAAAGAAGGTGATCGCGTCATCACCGA
AGGCCTGCAGTACGTCAAACCAGGCGCCGAAGTGAAAGTCGCCGAAGCGACCAACGTCAAAGGAGCAAACCCGGCACCTG
CCCCGGCCACTGATAAAGCCGCAGGCAGCAAAGGGGAGTAA

Upstream 100 bases:

>100_bases
AATGTAAGTATATTCCTTAGCATGCTACTTATCTAGCCGAAATATTTTTTAACACTTCAACGTACTGTTGAGAAAACAGG
CTCCTGCCCCGAGGATCTTC

Downstream 100 bases:

>100_bases
CCCATGTCGAAATTTTTTATTGATCGACCCATTTTTGCCTGGGTTATTGCCCTCGTGATCATGCTGGTCGGTGCGCTGTC
GATTTCCAGCCTGCCGATCA

Product: secretion protein HlyD

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 386; Mature: 386

Protein sequence:

>386_residues
MQFKPAVTVLVTAIALATLLSGCSKKEQAAPPAQTPQVGVVTIKAQPYTLTTELPGRTTAFRVAEVRPQVNGIILKRLFT
EGGDVKAGQQLYQIDPAVYEANANSAKATLQSAKSMSDRYKQLVSEQAVSRQEYDTALASTQEAQAALQTAQINLRYTKV
LAPISGRIGRSAVTEGALVSSAQTDAMATIQQLDPIYVDVVQSSADMLKLRSDLDSGKLQKAGDNAAKVKLTLEDNSVYA
QEGKLEFSEVSVDQTTGSVTLRAVFPNPEHRLLPGMFVHAQLQAGVNSKAILAPQQGVTRDLKGTPTAMVVNQDNKVEMR
TLVANRTVGSDWLIEDGLKEGDRVITEGLQYVKPGAEVKVAEATNVKGANPAPAPATDKAAGSKGE

Sequences:

>Translated_386_residues
MQFKPAVTVLVTAIALATLLSGCSKKEQAAPPAQTPQVGVVTIKAQPYTLTTELPGRTTAFRVAEVRPQVNGIILKRLFT
EGGDVKAGQQLYQIDPAVYEANANSAKATLQSAKSMSDRYKQLVSEQAVSRQEYDTALASTQEAQAALQTAQINLRYTKV
LAPISGRIGRSAVTEGALVSSAQTDAMATIQQLDPIYVDVVQSSADMLKLRSDLDSGKLQKAGDNAAKVKLTLEDNSVYA
QEGKLEFSEVSVDQTTGSVTLRAVFPNPEHRLLPGMFVHAQLQAGVNSKAILAPQQGVTRDLKGTPTAMVVNQDNKVEMR
TLVANRTVGSDWLIEDGLKEGDRVITEGLQYVKPGAEVKVAEATNVKGANPAPAPATDKAAGSKGE
>Mature_386_residues
MQFKPAVTVLVTAIALATLLSGCSKKEQAAPPAQTPQVGVVTIKAQPYTLTTELPGRTTAFRVAEVRPQVNGIILKRLFT
EGGDVKAGQQLYQIDPAVYEANANSAKATLQSAKSMSDRYKQLVSEQAVSRQEYDTALASTQEAQAALQTAQINLRYTKV
LAPISGRIGRSAVTEGALVSSAQTDAMATIQQLDPIYVDVVQSSADMLKLRSDLDSGKLQKAGDNAAKVKLTLEDNSVYA
QEGKLEFSEVSVDQTTGSVTLRAVFPNPEHRLLPGMFVHAQLQAGVNSKAILAPQQGVTRDLKGTPTAMVVNQDNKVEMR
TLVANRTVGSDWLIEDGLKEGDRVITEGLQYVKPGAEVKVAEATNVKGANPAPAPATDKAAGSKGE

Specific function: The periplasmic linker protein component of an organic solvent and antibiotic efflux pump; confers resistance to toluene, hexane, p-xylene, ampicillin, penicillin G, erythromycin, novobiocin and tetracycline [H]

COG id: COG0845

COG function: function code M; Membrane-fusion protein

Gene ontology:

Cell location: Cell inner membrane; Lipid-anchor (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1786668, Length=376, Percent_Identity=54.7872340425532, Blast_Score=392, Evalue=1e-110,
Organism=Escherichia coli, GI1789665, Length=358, Percent_Identity=51.6759776536313, Blast_Score=362, Evalue=1e-101,
Organism=Escherichia coli, GI1789929, Length=365, Percent_Identity=43.2876712328767, Blast_Score=310, Evalue=9e-86,
Organism=Escherichia coli, GI87082036, Length=324, Percent_Identity=30.8641975308642, Blast_Score=127, Evalue=2e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 41044; Mature: 41044

Theoretical pI: Translated: 8.36; Mature: 8.36

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQFKPAVTVLVTAIALATLLSGCSKKEQAAPPAQTPQVGVVTIKAQPYTLTTELPGRTTA
CCCCHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCCEEEEEEEECCEEEEECCCCCCEE
FRVAEVRPQVNGIILKRLFTEGGDVKAGQQLYQIDPAVYEANANSAKATLQSAKSMSDRY
EEEEECCCCHHHHHHHHHHCCCCCCCCCCHHEECCCCEEECCCCHHHHHHHHHHHHHHHH
KQLVSEQAVSRQEYDTALASTQEAQAALQTAQINLRYTKVLAPISGRIGRSAVTEGALVS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEEECCCCCCCCCHHHCCCHHHC
SAQTDAMATIQQLDPIYVDVVQSSADMLKLRSDLDSGKLQKAGDNAAKVKLTLEDNSVYA
CCCHHHHHHHHHCCCCEEHHHHCHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEECCEEEE
QEGKLEFSEVSVDQTTGSVTLRAVFPNPEHRLLPGMFVHAQLQAGVNSKAILAPQQGVTR
CCCCEEEHHEEEECCCCCEEEEEECCCCCCCCCCCEEEEEEHHCCCCCCEEECCCCCCCC
DLKGTPTAMVVNQDNKVEMRTLVANRTVGSDWLIEDGLKEGDRVITEGLQYVKPGAEVKV
CCCCCCCEEEEECCCCEEEEEEHHCCCCCCCCHHHCCHHHHHHHHHHHHHHHCCCCCEEE
AEATNVKGANPAPAPATDKAAGSKGE
EECCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MQFKPAVTVLVTAIALATLLSGCSKKEQAAPPAQTPQVGVVTIKAQPYTLTTELPGRTTA
CCCCHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCCEEEEEEEECCEEEEECCCCCCEE
FRVAEVRPQVNGIILKRLFTEGGDVKAGQQLYQIDPAVYEANANSAKATLQSAKSMSDRY
EEEEECCCCHHHHHHHHHHCCCCCCCCCCHHEECCCCEEECCCCHHHHHHHHHHHHHHHH
KQLVSEQAVSRQEYDTALASTQEAQAALQTAQINLRYTKVLAPISGRIGRSAVTEGALVS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEEECCCCCCCCCHHHCCCHHHC
SAQTDAMATIQQLDPIYVDVVQSSADMLKLRSDLDSGKLQKAGDNAAKVKLTLEDNSVYA
CCCHHHHHHHHHCCCCEEHHHHCHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEECCEEEE
QEGKLEFSEVSVDQTTGSVTLRAVFPNPEHRLLPGMFVHAQLQAGVNSKAILAPQQGVTR
CCCCEEEHHEEEECCCCCEEEEEECCCCCCCCCCCEEEEEEHHCCCCCCEEECCCCCCCC
DLKGTPTAMVVNQDNKVEMRTLVANRTVGSDWLIEDGLKEGDRVITEGLQYVKPGAEVKV
CCCCCCCEEEEECCCCEEEEEEHHCCCCCCCCHHHCCHHHHHHHHHHHHHHHCCCCCEEE
AEATNVKGANPAPAPATDKAAGSKGE
EECCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9827328 [H]