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The map label for this gene is emrB [C]

Identifier: 66047231

GI number: 66047231

Start: 4767044

End: 4768540

Strand: Direct

Name: emrB [C]

Synonym: Psyr_4004

Alternate gene names: 66047231

Gene position: 4767044-4768540 (Clockwise)

Preceding gene: 66047229

Following gene: 66047234

Centisome position: 78.23

GC content: 62.06

Gene sequence:

>1497_bases
TTGACCAGTCTTAATCAAACGCCTCCGGCCATCCGCAGCATCCTGTTTGCGTTGATGATGGCAGTCCTGCTCAGCGCGCT
GGACCAAACCATCGTCGCGGTCTCGATGCCCGCCATCTCTGCCCAGTTCAGAGACATCGATCTACTGGCCTGGGTCATAT
CCGCCTACATGGTGTCGCTGACCGTGGCCGTGCCGATCTACGGCAAACTCGGCGATCTTTACGGGCGCCGTAAACTGATG
CTGTTCGGGCTGGGGATTTTTACCCTGGCGTCGCTGTTCTGTGGCCTGGCGCAGAGCATGGAACAACTGGTCCTGGCGCG
CGTCCTGCAAGGCATCGGCGCGGGCGGGATGGTTTCGGTCAGCCAGGCGATCATCGCCGACATCGTTCCACCTCGCGAAC
GTGGCCGCTATCAGGGTTATTTCAGCAGCATGTACGCCGTGGCCAGCGTCGCCGGGCCGGTGCTTGGCGGCCTGATGACT
GAATACCTGTCCTGGCGCTGGGTGTTCCTGATCAATCTGCCATTGGGGGCCGCTGCGCTGATCGTCGCGTATCGGACCCT
GGTTGGCCTGCCGGTGCCGCAGCGCAAGCCGATCATCGATTACCTGGGTACCGTGCTGATGATCATCGGCCTGACAGCGC
TGCTGTTGGGGATTACCGAAATTGGCCAGGGTCATGGCCTGGGCGATGCCGAGGTTCAGTTGTTACTGGGCACGGCCCTG
CTGACGCTGGCCATTTTCGTCTGGTACGAACGTCGCACTGCCGAACCGTTGCTGCCCATGCATCTGTTCACCAACAAGAG
CGCCGTGCTGTGCTGGTGCACCGTGTTCTTCACCAGTTTTCAGGCCATCTCCCTGATTGTCCTGATGCCGCTGCGTTACC
AGACCGTTACCGGGGGCGGCGCAGACAGCGCCGCCCTGCACTTGCTTCCACTGGCGATTGGCATGCCGATGGGCGCCTAT
TTTGCCGGGCGACGCACCGCTCATACCGGGCGCTACAAGCCGCTGATCCTGACCGGTGCGCTGTTGATGCCGGTTGCGAT
TCTGGGCATGGCCTTCACGCCGCCGCAATCGCTGATAGTGATGAGCCTGTTCATGGTCCTCACCGGTGTTGCGACCGGCA
TGCAGTTCCCCACGTCATTGGTCGGCACTCAGAATTCGGTTCAGCCCCGGGATATGGGCGTGGCGACCAGCACGACCAAT
CTGTTCCGCTCACTGGGTGGTGCAGTGGGCGTGGCACTGATGTCGGCGCTGCTGTTGGCCATGTTGCAGCACACCGGGGT
CGGCCAACTCGGCGCAGGAGCGCTGGGTAGCGAAGGCAGCTCCGGCAACGTCCTGCTCGACAGTCTGAACGCGGCAACCG
GCCCCGCGCTGGAAACCCTGCGCACCGAACTGGCAGTGACCTTCAGAAACCTGCTGATCACCAGCGCGGCCATTTCGCTG
CTCGGTCTTGCGGCAGCCGCAGTGATGCCCAATACATTATTGCGAGGACGGGACTGA

Upstream 100 bases:

>100_bases
ATTGCGCAAAGGCAGCTATCGATTAGCAAGCTATCGGATTAGACTGGCCAGACTCTCCCGCCACACTTGCGGCGGGTCAA
CACTGCCATCAGGTTTTGCC

Downstream 100 bases:

>100_bases
TCGGCCTTTCCGGCTCAGGAAGGCAACCCGGCCCAGAACCATTGCGTTTTCTTGCTCTTTATATGAGCAAACGCAGGCAT
CAGTTCACCCTCGTCGAAGT

Product: EmrB/QacA family drug resistance transporter

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 498; Mature: 497

Protein sequence:

>498_residues
MTSLNQTPPAIRSILFALMMAVLLSALDQTIVAVSMPAISAQFRDIDLLAWVISAYMVSLTVAVPIYGKLGDLYGRRKLM
LFGLGIFTLASLFCGLAQSMEQLVLARVLQGIGAGGMVSVSQAIIADIVPPRERGRYQGYFSSMYAVASVAGPVLGGLMT
EYLSWRWVFLINLPLGAAALIVAYRTLVGLPVPQRKPIIDYLGTVLMIIGLTALLLGITEIGQGHGLGDAEVQLLLGTAL
LTLAIFVWYERRTAEPLLPMHLFTNKSAVLCWCTVFFTSFQAISLIVLMPLRYQTVTGGGADSAALHLLPLAIGMPMGAY
FAGRRTAHTGRYKPLILTGALLMPVAILGMAFTPPQSLIVMSLFMVLTGVATGMQFPTSLVGTQNSVQPRDMGVATSTTN
LFRSLGGAVGVALMSALLLAMLQHTGVGQLGAGALGSEGSSGNVLLDSLNAATGPALETLRTELAVTFRNLLITSAAISL
LGLAAAAVMPNTLLRGRD

Sequences:

>Translated_498_residues
MTSLNQTPPAIRSILFALMMAVLLSALDQTIVAVSMPAISAQFRDIDLLAWVISAYMVSLTVAVPIYGKLGDLYGRRKLM
LFGLGIFTLASLFCGLAQSMEQLVLARVLQGIGAGGMVSVSQAIIADIVPPRERGRYQGYFSSMYAVASVAGPVLGGLMT
EYLSWRWVFLINLPLGAAALIVAYRTLVGLPVPQRKPIIDYLGTVLMIIGLTALLLGITEIGQGHGLGDAEVQLLLGTAL
LTLAIFVWYERRTAEPLLPMHLFTNKSAVLCWCTVFFTSFQAISLIVLMPLRYQTVTGGGADSAALHLLPLAIGMPMGAY
FAGRRTAHTGRYKPLILTGALLMPVAILGMAFTPPQSLIVMSLFMVLTGVATGMQFPTSLVGTQNSVQPRDMGVATSTTN
LFRSLGGAVGVALMSALLLAMLQHTGVGQLGAGALGSEGSSGNVLLDSLNAATGPALETLRTELAVTFRNLLITSAAISL
LGLAAAAVMPNTLLRGRD
>Mature_497_residues
TSLNQTPPAIRSILFALMMAVLLSALDQTIVAVSMPAISAQFRDIDLLAWVISAYMVSLTVAVPIYGKLGDLYGRRKLML
FGLGIFTLASLFCGLAQSMEQLVLARVLQGIGAGGMVSVSQAIIADIVPPRERGRYQGYFSSMYAVASVAGPVLGGLMTE
YLSWRWVFLINLPLGAAALIVAYRTLVGLPVPQRKPIIDYLGTVLMIIGLTALLLGITEIGQGHGLGDAEVQLLLGTALL
TLAIFVWYERRTAEPLLPMHLFTNKSAVLCWCTVFFTSFQAISLIVLMPLRYQTVTGGGADSAALHLLPLAIGMPMGAYF
AGRRTAHTGRYKPLILTGALLMPVAILGMAFTPPQSLIVMSLFMVLTGVATGMQFPTSLVGTQNSVQPRDMGVATSTTNL
FRSLGGAVGVALMSALLLAMLQHTGVGQLGAGALGSEGSSGNVLLDSLNAATGPALETLRTELAVTFRNLLITSAAISLL
GLAAAAVMPNTLLRGRD

Specific function: Translocase That Confers Resistance To Substances Of High Hydrophobicity. [C]

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. TCR/tet family [H]

Homologues:

Organism=Escherichia coli, GI1789042, Length=407, Percent_Identity=27.027027027027, Blast_Score=146, Evalue=4e-36,
Organism=Escherichia coli, GI87081983, Length=425, Percent_Identity=29.6470588235294, Blast_Score=142, Evalue=7e-35,
Organism=Escherichia coli, GI1788710, Length=410, Percent_Identity=27.5609756097561, Blast_Score=123, Evalue=2e-29,
Organism=Escherichia coli, GI1790195, Length=296, Percent_Identity=29.3918918918919, Blast_Score=122, Evalue=6e-29,
Organism=Escherichia coli, GI1788392, Length=312, Percent_Identity=27.8846153846154, Blast_Score=102, Evalue=4e-23,
Organism=Escherichia coli, GI1790146, Length=169, Percent_Identity=28.9940828402367, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1788509, Length=158, Percent_Identity=29.1139240506329, Blast_Score=65, Evalue=7e-12,
Organism=Saccharomyces cerevisiae, GI6319770, Length=460, Percent_Identity=28.0434782608696, Blast_Score=142, Evalue=1e-34,
Organism=Saccharomyces cerevisiae, GI6323735, Length=444, Percent_Identity=27.027027027027, Blast_Score=120, Evalue=4e-28,
Organism=Saccharomyces cerevisiae, GI6325455, Length=452, Percent_Identity=25, Blast_Score=114, Evalue=3e-26,
Organism=Saccharomyces cerevisiae, GI6322958, Length=369, Percent_Identity=25.4742547425474, Blast_Score=114, Evalue=4e-26,
Organism=Saccharomyces cerevisiae, GI6321663, Length=504, Percent_Identity=24.6031746031746, Blast_Score=102, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6319783, Length=286, Percent_Identity=25.5244755244755, Blast_Score=80, Evalue=7e-16,
Organism=Saccharomyces cerevisiae, GI6324954, Length=241, Percent_Identity=26.5560165975104, Blast_Score=75, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6324264, Length=166, Percent_Identity=25.3012048192771, Blast_Score=64, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR005829
- InterPro:   IPR001958
- InterPro:   IPR011991 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 52558; Mature: 52427

Theoretical pI: Translated: 9.58; Mature: 9.58

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSLNQTPPAIRSILFALMMAVLLSALDQTIVAVSMPAISAQFRDIDLLAWVISAYMVSL
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
TVAVPIYGKLGDLYGRRKLMLFGLGIFTLASLFCGLAQSMEQLVLARVLQGIGAGGMVSV
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHH
SQAIIADIVPPRERGRYQGYFSSMYAVASVAGPVLGGLMTEYLSWRWVFLINLPLGAAAL
HHHHHHHCCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEEEECCHHHHHH
IVAYRTLVGLPVPQRKPIIDYLGTVLMIIGLTALLLGITEIGQGHGLGDAEVQLLLGTAL
HHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHH
LTLAIFVWYERRTAEPLLPMHLFTNKSAVLCWCTVFFTSFQAISLIVLMPLRYQTVTGGG
HHHHHHHHHHHCCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCC
ADSAALHLLPLAIGMPMGAYFAGRRTAHTGRYKPLILTGALLMPVAILGMAFTPPQSLIV
CCHHHHHHHHHHHCCCHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHH
MSLFMVLTGVATGMQFPTSLVGTQNSVQPRDMGVATSTTNLFRSLGGAVGVALMSALLLA
HHHHHHHHHHHHCCCCCHHHHCCCCCCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
MLQHTGVGQLGAGALGSEGSSGNVLLDSLNAATGPALETLRTELAVTFRNLLITSAAISL
HHHHCCCCCCCCCCCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LGLAAAAVMPNTLLRGRD
HHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TSLNQTPPAIRSILFALMMAVLLSALDQTIVAVSMPAISAQFRDIDLLAWVISAYMVSL
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH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HHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]