Click here to switch to the map view.

The map label for this gene is rnt [H]

Identifier: 66047122

GI number: 66047122

Start: 4636903

End: 4637580

Strand: Direct

Name: rnt [H]

Synonym: Psyr_3895

Alternate gene names: 66047122

Gene position: 4636903-4637580 (Clockwise)

Preceding gene: 66047121

Following gene: 66047123

Centisome position: 76.09

GC content: 61.65

Gene sequence:

>678_bases
GTGAGTGAAGATCATTTTGACGACGAACACGAAGGCCACGGCGGTGGCGGCGGTTCCCGTCACCCGATGGCGGCGCGCTT
TCGCGGCTACCTGCCAGTGGTCGTCGACGTAGAAACCGGCGGCTTCAATTCAGCCACCGATGCCTTGCTGGAAATCGCTG
CGGTGACGATCGGCATGGATGAAAAAGGCTTCGTGTTCCCGGAACACACCTATTTCTTCCGCGTAGAACCGTTCGAGGGT
GCCAACATCGAAGCGGCGGCGCTGGAGTTCACCGGCATCAAGCTCGATCATCCGCTGCGCATGGCGGTCAGCGAAGAAAC
CGCGATGAACGACATCTTCCGCGGTGTGCGCAAGGCCCTCAAGGCCAATGGCTGCAAACGCGCGGTGCTGGTCGGCCACA
ACGCCAGCTTCGACCTCGGCTTCGTCAATGCCGCCGTCGCACGGATGGACATGAAGCGCAATCCGTTTCACCCGTTCTCC
AGCTTCGACACCGCCACCCTCGCCGGCCTGGCCTATGGTCAGACCGTGCTGGCAAAGGCCTGTCAGGCGGCAGGCATCGA
CTTCGACGGACGCGAGGCGCACTCGGCCCGTTACGACACCGAGAAGACCGCCGAGCTGTTCTGCGGCATCGTCAATCGCT
GGAAAGAAATGGGCGGTTGGCAAGATTTCGACGACTGA

Upstream 100 bases:

>100_bases
GTGACGAGTGGACCGCCCCTGCCAGCCTGCCGTTTGGCGAGTTGACCGTTATCCCGCTGCGCGCAGGTGAAACACTGCGC
TGGCGTCTGGAGGAACACGC

Downstream 100 bases:

>100_bases
CGTCGTTACGTCTGTGAATGGACCGGCCTTGCGCCGGTTTTTTCATGTCTGCAGCGGGACGATCCAGAGGGCGGACAAAT
GATGCGATTCTCGCGAAAGC

Product: ribonuclease T

Products: NA

Alternate protein names: Exoribonuclease T; RNase T [H]

Number of amino acids: Translated: 225; Mature: 224

Protein sequence:

>225_residues
MSEDHFDDEHEGHGGGGGSRHPMAARFRGYLPVVVDVETGGFNSATDALLEIAAVTIGMDEKGFVFPEHTYFFRVEPFEG
ANIEAAALEFTGIKLDHPLRMAVSEETAMNDIFRGVRKALKANGCKRAVLVGHNASFDLGFVNAAVARMDMKRNPFHPFS
SFDTATLAGLAYGQTVLAKACQAAGIDFDGREAHSARYDTEKTAELFCGIVNRWKEMGGWQDFDD

Sequences:

>Translated_225_residues
MSEDHFDDEHEGHGGGGGSRHPMAARFRGYLPVVVDVETGGFNSATDALLEIAAVTIGMDEKGFVFPEHTYFFRVEPFEG
ANIEAAALEFTGIKLDHPLRMAVSEETAMNDIFRGVRKALKANGCKRAVLVGHNASFDLGFVNAAVARMDMKRNPFHPFS
SFDTATLAGLAYGQTVLAKACQAAGIDFDGREAHSARYDTEKTAELFCGIVNRWKEMGGWQDFDD
>Mature_224_residues
SEDHFDDEHEGHGGGGGSRHPMAARFRGYLPVVVDVETGGFNSATDALLEIAAVTIGMDEKGFVFPEHTYFFRVEPFEGA
NIEAAALEFTGIKLDHPLRMAVSEETAMNDIFRGVRKALKANGCKRAVLVGHNASFDLGFVNAAVARMDMKRNPFHPFSS
FDTATLAGLAYGQTVLAKACQAAGIDFDGREAHSARYDTEKTAELFCGIVNRWKEMGGWQDFDD

Specific function: Responsible for the end-turnover of tRNA:specifically removes the terminal AMP residue from uncharged tRNA (tRNA-C-C-A). Also appears to be involved in tRNA biosynthesis [H]

COG id: COG0847

COG function: function code L; DNA polymerase III, epsilon subunit and related 3'-5' exonucleases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 exonuclease domain [H]

Homologues:

Organism=Escherichia coli, GI1787941, Length=195, Percent_Identity=63.5897435897436, Blast_Score=266, Evalue=1e-72,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006055
- InterPro:   IPR013520
- InterPro:   IPR005987
- InterPro:   IPR012337 [H]

Pfam domain/function: PF00929 Exonuc_X-T [H]

EC number: 3.1.13.-

Molecular weight: Translated: 24588; Mature: 24457

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEDHFDDEHEGHGGGGGSRHPMAARFRGYLPVVVDVETGGFNSATDALLEIAAVTIGMD
CCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCC
EKGFVFPEHTYFFRVEPFEGANIEAAALEFTGIKLDHPLRMAVSEETAMNDIFRGVRKAL
CCCCCCCCCEEEEEECCCCCCCCEEEEEEEECEEECCCHHHHHHHHHHHHHHHHHHHHHH
KANGCKRAVLVGHNASFDLGFVNAAVARMDMKRNPFHPFSSFDTATLAGLAYGQTVLAKA
HCCCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
CQAAGIDFDGREAHSARYDTEKTAELFCGIVNRWKEMGGWQDFDD
HHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
SEDHFDDEHEGHGGGGGSRHPMAARFRGYLPVVVDVETGGFNSATDALLEIAAVTIGMD
CCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCC
EKGFVFPEHTYFFRVEPFEGANIEAAALEFTGIKLDHPLRMAVSEETAMNDIFRGVRKAL
CCCCCCCCCEEEEEECCCCCCCCEEEEEEEECEEECCCHHHHHHHHHHHHHHHHHHHHHH
KANGCKRAVLVGHNASFDLGFVNAAVARMDMKRNPFHPFSSFDTATLAGLAYGQTVLAKA
HCCCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
CQAAGIDFDGREAHSARYDTEKTAELFCGIVNRWKEMGGWQDFDD
HHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on ester bonds [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA