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The map label for this gene is argT [H]

Identifier: 66047101

GI number: 66047101

Start: 4615411

End: 4616193

Strand: Direct

Name: argT [H]

Synonym: Psyr_3874

Alternate gene names: 66047101

Gene position: 4615411-4616193 (Clockwise)

Preceding gene: 66047100

Following gene: 66047102

Centisome position: 75.74

GC content: 56.19

Gene sequence:

>783_bases
ATGAAAAAAGCATGGCTGACTCTTTCTGCACTGGCTCTGTGCATCGCCGCCGGTAACACCATGGCCAAGGAATACAAGGA
ACTGCGCTTCGGCGTCGATCCATCTTATGCACCGTTCGAATCCAAAGCAGCCGACGGCAGCCTGGTCGGCTTCGATATCG
ATCTGGGCAACGCAATCTGCAAAGAGCTGAAAGTCAAATGCAAATGGGTCGAAAGCGACTTCGACGGCATGATCCCCGGC
CTCAAGGCACGCAAATTCGACGGCGTTATCTCCTCGATGACCGTGACCCCTGCTCGCGAAAAAGTCATCTACTTCTCCAA
CGAGCTGTTCTCCGGCCCCACCTCGCTGGTGTTCAAGAAAGGTGCAGGCTTTACTGCCGACCCGGCCAGCCTGAAAGGCA
AGACCGTGGGCTATGAGCAAGGCACCATCCAGGAAGCCTACGCCAAGGCGGTACTGGACAAGGCCGGTATCTCCACCAAG
GCCTATGCCAACCAGGATCAAGTTTACTCCGACCTGACCTCGGGCCGTCTCGACGCCTCGATCCAGGACATGCTGCAGGC
CGAACTGGGCTTCCTGAAATCGCCAGCCGGTGCTGACTACGAAGTCAGCAAGCCTATCGACAGCGAACTGCTGCCAGCCA
AAACCGCCATCGGCATTGCCCAGGGCAACAAGGAACTCAAGGCGTTGCTGGACAAAGGCATCGAGGCGATGCACAAAGAC
GGCACCTACGCTGAAATCCAGAAAAAACACTTTGGCGACCTGAACCTCTACAGCGGTAAATAA

Upstream 100 bases:

>100_bases
CCTCTGGTTCGGAAATTGCTAAACAATGCCTATCTGGCACTTGCCGGTAGCACGCTTCGCGTATTCCCAACGCCCCATTT
AAGGTTTTAAGGACTGAACA

Downstream 100 bases:

>100_bases
CACCCCGACGCCCATCGATTTCGGCGATCATTCGTTGCCCGATCGGTGGGCGTTTTTGTTGGTAACAAGGCCTATCCATG
CTCGATGCACTCATGCAAAA

Product: extracellular solute-binding protein

Products: ADP; phosphate; L-ornithine [Cytoplasm]; ADP; L-arginine [Cytoplasm]; L-lysine [Cytoplasm] [C]

Alternate protein names: LAO-binding protein [H]

Number of amino acids: Translated: 260; Mature: 260

Protein sequence:

>260_residues
MKKAWLTLSALALCIAAGNTMAKEYKELRFGVDPSYAPFESKAADGSLVGFDIDLGNAICKELKVKCKWVESDFDGMIPG
LKARKFDGVISSMTVTPAREKVIYFSNELFSGPTSLVFKKGAGFTADPASLKGKTVGYEQGTIQEAYAKAVLDKAGISTK
AYANQDQVYSDLTSGRLDASIQDMLQAELGFLKSPAGADYEVSKPIDSELLPAKTAIGIAQGNKELKALLDKGIEAMHKD
GTYAEIQKKHFGDLNLYSGK

Sequences:

>Translated_260_residues
MKKAWLTLSALALCIAAGNTMAKEYKELRFGVDPSYAPFESKAADGSLVGFDIDLGNAICKELKVKCKWVESDFDGMIPG
LKARKFDGVISSMTVTPAREKVIYFSNELFSGPTSLVFKKGAGFTADPASLKGKTVGYEQGTIQEAYAKAVLDKAGISTK
AYANQDQVYSDLTSGRLDASIQDMLQAELGFLKSPAGADYEVSKPIDSELLPAKTAIGIAQGNKELKALLDKGIEAMHKD
GTYAEIQKKHFGDLNLYSGK
>Mature_260_residues
MKKAWLTLSALALCIAAGNTMAKEYKELRFGVDPSYAPFESKAADGSLVGFDIDLGNAICKELKVKCKWVESDFDGMIPG
LKARKFDGVISSMTVTPAREKVIYFSNELFSGPTSLVFKKGAGFTADPASLKGKTVGYEQGTIQEAYAKAVLDKAGISTK
AYANQDQVYSDLTSGRLDASIQDMLQAELGFLKSPAGADYEVSKPIDSELLPAKTAIGIAQGNKELKALLDKGIEAMHKD
GTYAEIQKKHFGDLNLYSGK

Specific function: This periplasmic binding protein is involved in an arginine transport system. ArgT and histidine-binding protein J (hisJ) interact with a common membrane-bound receptor, hisP [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1788649, Length=259, Percent_Identity=45.945945945946, Blast_Score=225, Evalue=2e-60,
Organism=Escherichia coli, GI1788648, Length=236, Percent_Identity=42.3728813559322, Blast_Score=202, Evalue=2e-53,
Organism=Escherichia coli, GI1787085, Length=251, Percent_Identity=33.4661354581673, Blast_Score=133, Evalue=1e-32,
Organism=Escherichia coli, GI1787088, Length=242, Percent_Identity=33.4710743801653, Blast_Score=132, Evalue=2e-32,
Organism=Escherichia coli, GI1787031, Length=247, Percent_Identity=31.1740890688259, Blast_Score=109, Evalue=1e-25,
Organism=Escherichia coli, GI1788228, Length=232, Percent_Identity=29.3103448275862, Blast_Score=97, Evalue=7e-22,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005768
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 27989; Mature: 27989

Theoretical pI: Translated: 7.34; Mature: 7.34

Prosite motif: PS01039 SBP_BACTERIAL_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKAWLTLSALALCIAAGNTMAKEYKELRFGVDPSYAPFESKAADGSLVGFDIDLGNAIC
CCCHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEECHHHHHH
KELKVKCKWVESDFDGMIPGLKARKFDGVISSMTVTPAREKVIYFSNELFSGPTSLVFKK
HHHHHEEEEECCCCCCCCCCCCHHHHHHHHHHEEECCCCCEEEEECCHHCCCCHHEEEEC
GAGFTADPASLKGKTVGYEQGTIQEAYAKAVLDKAGISTKAYANQDQVYSDLTSGRLDAS
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHCCHHHHHHHHHCCCCCHH
IQDMLQAELGFLKSPAGADYEVSKPIDSELLPAKTAIGIAQGNKELKALLDKGIEAMHKD
HHHHHHHHHHHHCCCCCCCEECCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHCC
GTYAEIQKKHFGDLNLYSGK
CCHHHHHHHHCCCCEECCCC
>Mature Secondary Structure
MKKAWLTLSALALCIAAGNTMAKEYKELRFGVDPSYAPFESKAADGSLVGFDIDLGNAIC
CCCHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEECHHHHHH
KELKVKCKWVESDFDGMIPGLKARKFDGVISSMTVTPAREKVIYFSNELFSGPTSLVFKK
HHHHHEEEEECCCCCCCCCCCCHHHHHHHHHHEEECCCCCEEEEECCHHCCCCHHEEEEC
GAGFTADPASLKGKTVGYEQGTIQEAYAKAVLDKAGISTKAYANQDQVYSDLTSGRLDAS
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHCCHHHHHHHHHCCCCCHH
IQDMLQAELGFLKSPAGADYEVSKPIDSELLPAKTAIGIAQGNKELKALLDKGIEAMHKD
HHHHHHHHHHHHCCCCCCCEECCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHCC
GTYAEIQKKHFGDLNLYSGK
CCHHHHHHHHCCCCEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-ornithine [Periplasm]; H2O; ATP; L-arginine [Periplasm]; L-lysine [Periplasm] [C]

Specific reaction: ATP + L-ornithine [Periplasm] + H2O = ADP + phosphate + L-ornithine [Cytoplasm] ATP + L-arginine [Periplasm] + H2O = ADP + phosphate + L-arginine [Cytoplasm] ATP + L-lysine [Periplasm] + H2O = ADP + phosphate + L-lysine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503; 3040734; 9298646 [H]