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The map label for this gene is entD [H]

Identifier: 66046942

GI number: 66046942

Start: 4399936

End: 4400670

Strand: Direct

Name: entD [H]

Synonym: Psyr_3713

Alternate gene names: 66046942

Gene position: 4399936-4400670 (Clockwise)

Preceding gene: 66046940

Following gene: 66046946

Centisome position: 72.2

GC content: 63.13

Gene sequence:

>735_bases
ATGCCGCCCATGACTGAACTATCCCACCTCCCCGCCTGCTGCCCGGAACTGCTCCGGCATTGGCCGCTGCCACAAGCCTT
GCCGGGAGCAGTACTGGTGAGTGGACATTTTGATCCGCTCAAGCTGGCAGACGGCGACTTTCAGCGTTGTGAGCTGCAAA
TGCCGGCGAGCATTCAGCGCTCGGTGGCCAAGCGTCAGACTGAATTTCTCGCCGGTCGTCTCTGCGCACGCGAAGCCATG
CGTCAGCTCGATGGCCGTCTGCACGTTCCTGCAGTGGGCGAAGACCGCGCGCCGGTGTGGCCCTCGGATGTCTGCGGTTC
GATCACTCACAGCACCGGCTGGGCCGCTGCCGTGGTTGCGCACAAGCAGCAGTGGCGCGGGTTGGGGCTGGACACGGAAA
ACCTGCTGAGCCATGACCGCGCCTCGCGGCTGGCCGGGGAAATTCTGACGGCGGCCGAGCTGGCAGAGATGGCGGCGGGT
CCGGAAGATCAGATCGCACTGCGCGTGACCCTGACTTTTTCGATCAAGGAAGCGCTGTTCAAGGCGCTCTACCCCATCGT
GCACAAGCGTTTCTATTTCGAAGACGCACAACTGCTGGAATGGTCCGCCGATGGCCACGCCCGGCTACAACTGCTGATCG
ACCTGTCCAGCGAGTGGCATGCGGGCAAGGAGCTGGACGGTCAGTTCAGTGTGCAGGACGATCACCTGTTGAGCCTGGTC
GCCGTCGAAGGCTGA

Upstream 100 bases:

>100_bases
TCGCCGAGAGGACGCAGAGCGTCCAGAAAGGCCTGCGACGCGGAGCGCCGCACGATAGTTACTGCTAGTGCCCTTCCCTC
CAACCCGTTCCCCCGGCAAA

Downstream 100 bases:

>100_bases
TCAGCCGGGCAGCGGACAATGCTCGCAACGCCCAACCCACTCGACCCGATGACTCAAACAGCACACACGCCGTTGCCTGC
GTGGTTCACCACCTCGCGCC

Product: 4'-phosphopantetheinyl transferase

Products: pyrophosphate; AMP; enterobactin; aryl carrier protein/isochorismatase; 3',5'-ADP; serine activating enzyme + [C]

Alternate protein names: Enterobactin synthase component D; Enterochelin synthase D [H]

Number of amino acids: Translated: 244; Mature: 243

Protein sequence:

>244_residues
MPPMTELSHLPACCPELLRHWPLPQALPGAVLVSGHFDPLKLADGDFQRCELQMPASIQRSVAKRQTEFLAGRLCAREAM
RQLDGRLHVPAVGEDRAPVWPSDVCGSITHSTGWAAAVVAHKQQWRGLGLDTENLLSHDRASRLAGEILTAAELAEMAAG
PEDQIALRVTLTFSIKEALFKALYPIVHKRFYFEDAQLLEWSADGHARLQLLIDLSSEWHAGKELDGQFSVQDDHLLSLV
AVEG

Sequences:

>Translated_244_residues
MPPMTELSHLPACCPELLRHWPLPQALPGAVLVSGHFDPLKLADGDFQRCELQMPASIQRSVAKRQTEFLAGRLCAREAM
RQLDGRLHVPAVGEDRAPVWPSDVCGSITHSTGWAAAVVAHKQQWRGLGLDTENLLSHDRASRLAGEILTAAELAEMAAG
PEDQIALRVTLTFSIKEALFKALYPIVHKRFYFEDAQLLEWSADGHARLQLLIDLSSEWHAGKELDGQFSVQDDHLLSLV
AVEG
>Mature_243_residues
PPMTELSHLPACCPELLRHWPLPQALPGAVLVSGHFDPLKLADGDFQRCELQMPASIQRSVAKRQTEFLAGRLCAREAMR
QLDGRLHVPAVGEDRAPVWPSDVCGSITHSTGWAAAVVAHKQQWRGLGLDTENLLSHDRASRLAGEILTAAELAEMAAGP
EDQIALRVTLTFSIKEALFKALYPIVHKRFYFEDAQLLEWSADGHARLQLLIDLSSEWHAGKELDGQFSVQDDHLLSLVA
VEG

Specific function: Catalyzes the transfer of the 4'-phosphopantetheine moiety from coenzyme A to apo-domains of both entB (an ArCP domain) and entF (a PCP domain). Plays an essential role in the assembly of the enterobactin [H]

COG id: COG2977

COG function: function code Q; Phosphopantetheinyl transferase component of siderophore synthetase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the P-Pant transferase superfamily. EntD family [H]

Homologues:

Organism=Escherichia coli, GI1786797, Length=204, Percent_Identity=36.7647058823529, Blast_Score=89, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008278
- InterPro:   IPR003542 [H]

Pfam domain/function: PF01648 ACPS [H]

EC number: 2.7.8.- [C]

Molecular weight: Translated: 26921; Mature: 26790

Theoretical pI: Translated: 5.73; Mature: 5.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPPMTELSHLPACCPELLRHWPLPQALPGAVLVSGHFDPLKLADGDFQRCELQMPASIQR
CCCHHHHHHCHHHHHHHHHHCCCCCCCCCEEEEECCCCCCEECCCCHHEEEECCCHHHHH
SVAKRQTEFLAGRLCAREAMRQLDGRLHVPAVGEDRAPVWPSDVCGSITHSTGWAAAVVA
HHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCCCCCCCCCHHHHCCCCCCCCHHHHHHH
HKQQWRGLGLDTENLLSHDRASRLAGEILTAAELAEMAAGPEDQIALRVTLTFSIKEALF
HHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEHHHHHHHH
KALYPIVHKRFYFEDAQLLEWSADGHARLQLLIDLSSEWHAGKELDGQFSVQDDHLLSLV
HHHHHHHHHHHHCCCCHHEEECCCCCEEEEEEEECCCCCCCCCCCCCCEECCCCCEEEEE
AVEG
EECC
>Mature Secondary Structure 
PPMTELSHLPACCPELLRHWPLPQALPGAVLVSGHFDPLKLADGDFQRCELQMPASIQR
CCHHHHHHCHHHHHHHHHHCCCCCCCCCEEEEECCCCCCEECCCCHHEEEECCCHHHHH
SVAKRQTEFLAGRLCAREAMRQLDGRLHVPAVGEDRAPVWPSDVCGSITHSTGWAAAVVA
HHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCCCCCCCCCHHHHCCCCCCCCHHHHHHH
HKQQWRGLGLDTENLLSHDRASRLAGEILTAAELAEMAAGPEDQIALRVTLTFSIKEALF
HHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEHHHHHHHH
KALYPIVHKRFYFEDAQLLEWSADGHARLQLLIDLSSEWHAGKELDGQFSVQDDHLLSLV
HHHHHHHHHHHHCCCCHHEEECCCCCEEEEEEEECCCCCCCCCCCCCCEECCCCCEEEEE
AVEG
EECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 6 ATP; L-serine; 2,3-dihydroxybenzoate; Coenzyme A; apo-EntB; apo-serine activating enzyme [C]

Specific reaction: 6 ATP + 3 L-serine + 3 2,3-dihydroxybenzoate = 6 pyrophosphate + 6 AMP + enterobactin Coenzyme A + apo-EntB = aryl carrier protein/isochorismatase + 3',5'-ADP Coenzyme A + apo-serine activating enzyme = 3',5'-ADP + serine activating enzyme [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]