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The map label for this gene is usf [H]

Identifier: 66046940

GI number: 66046940

Start: 4397623

End: 4398411

Strand: Direct

Name: usf [H]

Synonym: Psyr_3711

Alternate gene names: 66046940

Gene position: 4397623-4398411 (Clockwise)

Preceding gene: 66046935

Following gene: 66046942

Centisome position: 72.17

GC content: 61.22

Gene sequence:

>789_bases
ATGCGTGTGTGGATTGCAATGATCATGATGGGCCTGACCGGCCTCGCCCAGGCGGCGATCAAGACCGAGCAGATCGATTA
CAAAAGTGCCGACGGCACCAAGCTGGTGGGCTATTACGCGTACGACGACGCGGTAAAAGGCCCGCGCCCTGGCGTCCTGG
TGGTCCACGAGTGGTGGGGCCTGAATGACTACGCCAAGCGCCGCGCCCGCGATCTGGCTGCACTGGGTTACAGCGCAATG
GCCATCGACATGTACGGCGAAGGCAAAAACACCGAGCACCCGAAAGACGCGATGGCCTTCATGCAGGCCGCGCTGAAGGA
CAGCGATGCCGCCGACAAGCGCTTTGACGCCGGGCTGGAGCAATTGAAGAAGCAGCCACAGACCGATCCGAAGAAGATCG
CAGCGATCGGTTACTGCTTCGGCGGCAAGATCGTGCTCGATGCGGCACGCCGCGGTGAACCGCTGCTGGGCGTGGTGAGC
TTCCACGGCGCACTGGTCACCAACACTCCGGCTAAACCGGGCATCAAGGTGCCAATGCTGGTCGAACACGGCGCCAAGGA
CAGCATGGTCACCCCTGAAAACGTCACCGCCTTCAAGAAGGAAATGGACGACGCCAAGGCCGACTACAAGTTCGTCAGCA
TCGACGGCGCCAAACACGGCTTCACCAACCCCGACGCCGACCGCCTGAGCCACGGCGAACACGGCGGCCCGGACATCGGC
TACAGCAAATCCGCCGATGAGAGCTCCTGGGCGGACATGAAAGAGTTCTTCAAGAAGATATTTGGTTGA

Upstream 100 bases:

>100_bases
CTGAACACTGCATGAACCGGCGCTGAACAGTCATCCACACCCTGTGCAGCAGAATCGGCTATGGTTATTTGCCAACTCAA
ACATCAACAAGGAGCTGACC

Downstream 100 bases:

>100_bases
CTGTCAGATGTTCCTTCTACACGGAGCGTCGGCACGATAGTCGGGATCATCGTTCCTCACGCTCCAGCGTGGGAATGCAG
GTCTCGACGCTCTGCGTCGC

Product: dienelactone hydrolase

Products: oxohex-2-enedioate [C]

Alternate protein names: NA

Number of amino acids: Translated: 262; Mature: 262

Protein sequence:

>262_residues
MRVWIAMIMMGLTGLAQAAIKTEQIDYKSADGTKLVGYYAYDDAVKGPRPGVLVVHEWWGLNDYAKRRARDLAALGYSAM
AIDMYGEGKNTEHPKDAMAFMQAALKDSDAADKRFDAGLEQLKKQPQTDPKKIAAIGYCFGGKIVLDAARRGEPLLGVVS
FHGALVTNTPAKPGIKVPMLVEHGAKDSMVTPENVTAFKKEMDDAKADYKFVSIDGAKHGFTNPDADRLSHGEHGGPDIG
YSKSADESSWADMKEFFKKIFG

Sequences:

>Translated_262_residues
MRVWIAMIMMGLTGLAQAAIKTEQIDYKSADGTKLVGYYAYDDAVKGPRPGVLVVHEWWGLNDYAKRRARDLAALGYSAM
AIDMYGEGKNTEHPKDAMAFMQAALKDSDAADKRFDAGLEQLKKQPQTDPKKIAAIGYCFGGKIVLDAARRGEPLLGVVS
FHGALVTNTPAKPGIKVPMLVEHGAKDSMVTPENVTAFKKEMDDAKADYKFVSIDGAKHGFTNPDADRLSHGEHGGPDIG
YSKSADESSWADMKEFFKKIFG
>Mature_262_residues
MRVWIAMIMMGLTGLAQAAIKTEQIDYKSADGTKLVGYYAYDDAVKGPRPGVLVVHEWWGLNDYAKRRARDLAALGYSAM
AIDMYGEGKNTEHPKDAMAFMQAALKDSDAADKRFDAGLEQLKKQPQTDPKKIAAIGYCFGGKIVLDAARRGEPLLGVVS
FHGALVTNTPAKPGIKVPMLVEHGAKDSMVTPENVTAFKKEMDDAKADYKFVSIDGAKHGFTNPDADRLSHGEHGGPDIG
YSKSADESSWADMKEFFKKIFG

Specific function: Unknown

COG id: COG0412

COG function: function code Q; Dienelactone hydrolase and related enzymes

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI48994982, Length=245, Percent_Identity=23.6734693877551, Blast_Score=68, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI17532655, Length=247, Percent_Identity=34.8178137651822, Blast_Score=139, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI17532653, Length=247, Percent_Identity=33.1983805668016, Blast_Score=120, Evalue=8e-28,
Organism=Caenorhabditis elegans, GI17536419, Length=259, Percent_Identity=30.8880308880309, Blast_Score=116, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI193204305, Length=214, Percent_Identity=32.2429906542056, Blast_Score=108, Evalue=4e-24,
Organism=Caenorhabditis elegans, GI17534829, Length=155, Percent_Identity=36.1290322580645, Blast_Score=88, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI17536415, Length=247, Percent_Identity=28.7449392712551, Blast_Score=85, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002925 [H]

Pfam domain/function: PF01738 DLH [H]

EC number: 3.1.1.45 [C]

Molecular weight: Translated: 28583; Mature: 28583

Theoretical pI: Translated: 7.23; Mature: 7.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVWIAMIMMGLTGLAQAAIKTEQIDYKSADGTKLVGYYAYDDAVKGPRPGVLVVHEWWG
CHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEECCCCCCCCCCEEEEECCCC
LNDYAKRRARDLAALGYSAMAIDMYGEGKNTEHPKDAMAFMQAALKDSDAADKRFDAGLE
CCHHHHHHHHHHHHHCCEEEEEEEECCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHH
QLKKQPQTDPKKIAAIGYCFGGKIVLDAARRGEPLLGVVSFHGALVTNTPAKPGIKVPML
HHHHCCCCCHHHEEEEEEEECCEEEEEHHHCCCCEEEEEECCCEEEECCCCCCCCCCCHH
VEHGAKDSMVTPENVTAFKKEMDDAKADYKFVSIDGAKHGFTNPDADRLSHGEHGGPDIG
EECCCCCCCCCCCHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCHHHHCCCCCCCCCCC
YSKSADESSWADMKEFFKKIFG
CCCCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MRVWIAMIMMGLTGLAQAAIKTEQIDYKSADGTKLVGYYAYDDAVKGPRPGVLVVHEWWG
CHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEECCCCCCCCCCEEEEECCCC
LNDYAKRRARDLAALGYSAMAIDMYGEGKNTEHPKDAMAFMQAALKDSDAADKRFDAGLE
CCHHHHHHHHHHHHHCCEEEEEEEECCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHH
QLKKQPQTDPKKIAAIGYCFGGKIVLDAARRGEPLLGVVSFHGALVTNTPAKPGIKVPML
HHHHCCCCCHHHEEEEEEEECCEEEEEHHHCCCCEEEEEECCCEEEECCCCCCCCCCCHH
VEHGAKDSMVTPENVTAFKKEMDDAKADYKFVSIDGAKHGFTNPDADRLSHGEHGGPDIG
EECCCCCCCCCCCHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCHHHHCCCCCCCCCCC
YSKSADESSWADMKEFFKKIFG
CCCCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 4-carboxymethylenebut-2-en-4-olide; H2O [C]

Specific reaction: 4-carboxymethylenebut-2-en-4-olide + H2O = 4 oxohex-2-enedioate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7592443 [H]