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The map label for this gene is ttcA [H]

Identifier: 66046934

GI number: 66046934

Start: 4391725

End: 4392549

Strand: Reverse

Name: ttcA [H]

Synonym: Psyr_3705

Alternate gene names: 66046934

Gene position: 4392549-4391725 (Counterclockwise)

Preceding gene: 66046936

Following gene: 66046930

Centisome position: 72.08

GC content: 58.06

Gene sequence:

>825_bases
ATGGGCACCCTTTCAGTCAATCAGAACAAACTGCAGAAACGTCTGCGTCGGCTGGCCGGCGAAGCGGTTGCCGACTTCAA
CATGATCGAAGAGGGTGACAAGGTCATGGTTTGCCTGTCCGGCGGCAAGGACAGTTACACCATGCTCGATGTGCTGATGC
ACCTGCAGAAGGTCGCGCCGATCAAGTTCGACATCGTGGCGGTCAACATGGACCAGAAGCAGCCCGGCTTTCCTGAACAC
GTGCTGCCTGCCTATCTGAAAGAACTGGGTATCGAGTACCACATCGTCGAGAAAGACACGTATTCAGTGGTCAAGGAATT
GATCCCGGAAGGCAAGACCACCTGCTCCCTGTGTTCACGGCTGCGTCGCGGCACGCTGTACACCTTCGCTGACGAGATCG
GCGCCACCAAGATGGCGCTCGGGCACCACCGTGACGACATCGTCGAGACATTCTTTCTCAACATGTTCTTCAATGGCTCG
CTCAAGGCCATGCCGCCCAAGCTGCGTGCCGATGACGGCCGCAACGTGGTGATTCGTCCGTTGGCGTACTGCCACGAGAA
GGACATCCAGGCCTACTCGGACCTCAAGCAGTTTCCGATCATCCCGTGCAACCTCTGCGGCTCGCAGGAAAACCTTCAGC
GTCAGGTGGTCAAGGAGATGCTGCAGGACTGGGAGCGCAAGACACCGGGCCGCACGGAGAGCATCTTCCGCGCCCTGCAG
AACGTGCAGCCCTCGCAGCTGGCCGATCGCAACCTGTTCGACTTCACCAATCTGCGCATCGACGAAACCGCAGCATCACG
GTTCGTCAATGTGGTGAATATCTGA

Upstream 100 bases:

>100_bases
GCCTGTCTGAACAGCACGATCGCAGCATATTTAACGAATGGTCGGTTACGGGTAAAATGCCCGGCTTTTCTCAAGCTATC
GTCAGCGGATATTTCCATAC

Downstream 100 bases:

>100_bases
CAATGCTCAGTGGTTGACGGTGAACGCCAGCATGGCTGACAGCTGGCACATTGGCCGACCGCTTTCTGCATGCCACTGGT
TGAAGACGTCCTGCACGATC

Product: C32 tRNA thiolase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 274; Mature: 273

Protein sequence:

>274_residues
MGTLSVNQNKLQKRLRRLAGEAVADFNMIEEGDKVMVCLSGGKDSYTMLDVLMHLQKVAPIKFDIVAVNMDQKQPGFPEH
VLPAYLKELGIEYHIVEKDTYSVVKELIPEGKTTCSLCSRLRRGTLYTFADEIGATKMALGHHRDDIVETFFLNMFFNGS
LKAMPPKLRADDGRNVVIRPLAYCHEKDIQAYSDLKQFPIIPCNLCGSQENLQRQVVKEMLQDWERKTPGRTESIFRALQ
NVQPSQLADRNLFDFTNLRIDETAASRFVNVVNI

Sequences:

>Translated_274_residues
MGTLSVNQNKLQKRLRRLAGEAVADFNMIEEGDKVMVCLSGGKDSYTMLDVLMHLQKVAPIKFDIVAVNMDQKQPGFPEH
VLPAYLKELGIEYHIVEKDTYSVVKELIPEGKTTCSLCSRLRRGTLYTFADEIGATKMALGHHRDDIVETFFLNMFFNGS
LKAMPPKLRADDGRNVVIRPLAYCHEKDIQAYSDLKQFPIIPCNLCGSQENLQRQVVKEMLQDWERKTPGRTESIFRALQ
NVQPSQLADRNLFDFTNLRIDETAASRFVNVVNI
>Mature_273_residues
GTLSVNQNKLQKRLRRLAGEAVADFNMIEEGDKVMVCLSGGKDSYTMLDVLMHLQKVAPIKFDIVAVNMDQKQPGFPEHV
LPAYLKELGIEYHIVEKDTYSVVKELIPEGKTTCSLCSRLRRGTLYTFADEIGATKMALGHHRDDIVETFFLNMFFNGSL
KAMPPKLRADDGRNVVIRPLAYCHEKDIQAYSDLKQFPIIPCNLCGSQENLQRQVVKEMLQDWERKTPGRTESIFRALQN
VQPSQLADRNLFDFTNLRIDETAASRFVNVVNI

Specific function: Required for the thiolation of cytidine in position 32 of tRNA, to form 2-thiocytidine (s(2)C32) [H]

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TtcA family [H]

Homologues:

Organism=Escherichia coli, GI1787606, Length=264, Percent_Identity=69.6969696969697, Blast_Score=403, Evalue=1e-114,
Organism=Caenorhabditis elegans, GI193211090, Length=224, Percent_Identity=33.9285714285714, Blast_Score=131, Evalue=4e-31,
Organism=Caenorhabditis elegans, GI17540090, Length=232, Percent_Identity=26.7241379310345, Blast_Score=79, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24651972, Length=218, Percent_Identity=26.1467889908257, Blast_Score=70, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012089
- InterPro:   IPR014729
- InterPro:   IPR011063 [H]

Pfam domain/function: PF01171 ATP_bind_3 [H]

EC number: NA

Molecular weight: Translated: 31170; Mature: 31039

Theoretical pI: Translated: 7.46; Mature: 7.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGTLSVNQNKLQKRLRRLAGEAVADFNMIEEGDKVMVCLSGGKDSYTMLDVLMHLQKVAP
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHHCC
IKFDIVAVNMDQKQPGFPEHVLPAYLKELGIEYHIVEKDTYSVVKELIPEGKTTCSLCSR
CEEEEEEEECCCCCCCCCHHHHHHHHHHCCCEEEEECCHHHHHHHHHCCCCCHHHHHHHH
LRRGTLYTFADEIGATKMALGHHRDDIVETFFLNMFFNGSLKAMPPKLRADDGRNVVIRP
HHCCCEEEHHHHHCCHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEHH
LAYCHEKDIQAYSDLKQFPIIPCNLCGSQENLQRQVVKEMLQDWERKTPGRTESIFRALQ
HHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
NVQPSQLADRNLFDFTNLRIDETAASRFVNVVNI
CCCHHHHHCCCCHHHHCCEECHHHHHHHHHHHCC
>Mature Secondary Structure 
GTLSVNQNKLQKRLRRLAGEAVADFNMIEEGDKVMVCLSGGKDSYTMLDVLMHLQKVAP
CCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHHCC
IKFDIVAVNMDQKQPGFPEHVLPAYLKELGIEYHIVEKDTYSVVKELIPEGKTTCSLCSR
CEEEEEEEECCCCCCCCCHHHHHHHHHHCCCEEEEECCHHHHHHHHHCCCCCHHHHHHHH
LRRGTLYTFADEIGATKMALGHHRDDIVETFFLNMFFNGSLKAMPPKLRADDGRNVVIRP
HHCCCEEEHHHHHCCHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEHH
LAYCHEKDIQAYSDLKQFPIIPCNLCGSQENLQRQVVKEMLQDWERKTPGRTESIFRALQ
HHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
NVQPSQLADRNLFDFTNLRIDETAASRFVNVVNI
CCCHHHHHCCCCHHHHCCEECHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA