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The map label for this gene is argT [H]

Identifier: 66046800

GI number: 66046800

Start: 4252424

End: 4253200

Strand: Reverse

Name: argT [H]

Synonym: Psyr_3571

Alternate gene names: 66046800

Gene position: 4253200-4252424 (Counterclockwise)

Preceding gene: 66046801

Following gene: 66046799

Centisome position: 69.8

GC content: 57.14

Gene sequence:

>777_bases
ATGAAAAAGCTCATGCTTATTGGCGCGATGGCGTTGTCCGTGCTCGCCCAGCCGATCTTTGCTGATGAAAAACCACTGAA
AATCGGTATCGAAGCGGCTTACCCTCCGTTCGCGTCCAAGGCCCCGGATGGCAGCATCGTCGGCTTTGACTACGACATCG
GCAATGCGCTGTGTGAAGAAATGAAGGTCAAGTGCACCTGGGTCGAGCAGGAATTCGACGGGCTTATCCCGGCGCTGAAA
GTGCGCAAGATCGATGCGATCCTGTCGTCGATGTCCATCACCGACGACCGCAAGAAGTCCGTAGACTTCACCGGCAAGTA
TTACAATTCACCGGCGCGTCTGGTGATGAAGGACGGCACTACGGTCAGCGACAGCCTGGCTGAGCTCAAGGGCAAGAAGA
TCGGCGTACAGCGTGGCACGATTCACGAGCGTTTCGCCCGTGAAGTGCTGGCCCCGCAGGGTGTCGAAGTGACGCCGTAC
GGTTCGCAGAACGAAATCTATCTGGACATCAGTGCAGGGCGCCTTGACGGTACTATCGCCGACGCCACGCTGTTGCAGGA
CGGCTTCCTGAAAACCGATGCTGGCAAAGGCTATGCGTTCGTAGGCCCGTCGTTCTTCGATCCGAAATACTTCGGTGACG
GCATCGGCATTGCCGTGCGCAAGGGCGACAAGGCCAACCTTGACCGTCTCAACGCCGCCATCGCCGCGATCCGCGCCAAC
GGCAAGTACAAGGCCATCCAGGACAAGTATTTCGACTTCGATATCTTCGGCAAATAA

Upstream 100 bases:

>100_bases
TCACGCATCCGGCGTCGCTCCTCCCCGAGATTGTCCTTTCGCACATTGGGCGCTTGCTCACTTTGCCATTTGTCGTGCTT
TACCGATGGAGTCCCTGGAA

Downstream 100 bases:

>100_bases
GTCGTCGCAACGAGATGGCGCAAACAGGGTTGCCTGGCTTGCGCCATTTTTATTCCACGCTTGAGGACCGCACATCATGT
TGAAAGGTTACGGAGCTGTC

Product: lysine-arginine-ornithine-binding periplasmic protein

Products: ADP; phosphate; L-ornithine [Cytoplasm]; ADP; L-arginine [Cytoplasm]; L-lysine [Cytoplasm] [C]

Alternate protein names: LAO-binding protein [H]

Number of amino acids: Translated: 258; Mature: 258

Protein sequence:

>258_residues
MKKLMLIGAMALSVLAQPIFADEKPLKIGIEAAYPPFASKAPDGSIVGFDYDIGNALCEEMKVKCTWVEQEFDGLIPALK
VRKIDAILSSMSITDDRKKSVDFTGKYYNSPARLVMKDGTTVSDSLAELKGKKIGVQRGTIHERFAREVLAPQGVEVTPY
GSQNEIYLDISAGRLDGTIADATLLQDGFLKTDAGKGYAFVGPSFFDPKYFGDGIGIAVRKGDKANLDRLNAAIAAIRAN
GKYKAIQDKYFDFDIFGK

Sequences:

>Translated_258_residues
MKKLMLIGAMALSVLAQPIFADEKPLKIGIEAAYPPFASKAPDGSIVGFDYDIGNALCEEMKVKCTWVEQEFDGLIPALK
VRKIDAILSSMSITDDRKKSVDFTGKYYNSPARLVMKDGTTVSDSLAELKGKKIGVQRGTIHERFAREVLAPQGVEVTPY
GSQNEIYLDISAGRLDGTIADATLLQDGFLKTDAGKGYAFVGPSFFDPKYFGDGIGIAVRKGDKANLDRLNAAIAAIRAN
GKYKAIQDKYFDFDIFGK
>Mature_258_residues
MKKLMLIGAMALSVLAQPIFADEKPLKIGIEAAYPPFASKAPDGSIVGFDYDIGNALCEEMKVKCTWVEQEFDGLIPALK
VRKIDAILSSMSITDDRKKSVDFTGKYYNSPARLVMKDGTTVSDSLAELKGKKIGVQRGTIHERFAREVLAPQGVEVTPY
GSQNEIYLDISAGRLDGTIADATLLQDGFLKTDAGKGYAFVGPSFFDPKYFGDGIGIAVRKGDKANLDRLNAAIAAIRAN
GKYKAIQDKYFDFDIFGK

Specific function: This periplasmic binding protein is involved in an arginine transport system. ArgT and histidine-binding protein J (hisJ) interact with a common membrane-bound receptor, hisP [H]

COG id: COG0834

COG function: function code ET; ABC-type amino acid transport/signal transduction systems, periplasmic component/domain

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1788649, Length=259, Percent_Identity=43.6293436293436, Blast_Score=231, Evalue=4e-62,
Organism=Escherichia coli, GI1788648, Length=232, Percent_Identity=44.3965517241379, Blast_Score=218, Evalue=2e-58,
Organism=Escherichia coli, GI1787085, Length=251, Percent_Identity=41.4342629482072, Blast_Score=189, Evalue=1e-49,
Organism=Escherichia coli, GI1787088, Length=254, Percent_Identity=38.5826771653543, Blast_Score=174, Evalue=7e-45,
Organism=Escherichia coli, GI1788228, Length=263, Percent_Identity=31.1787072243346, Blast_Score=125, Evalue=3e-30,
Organism=Escherichia coli, GI1787031, Length=253, Percent_Identity=30.4347826086957, Blast_Score=111, Evalue=4e-26,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005768
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 28098; Mature: 28098

Theoretical pI: Translated: 8.14; Mature: 8.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKLMLIGAMALSVLAQPIFADEKPLKIGIEAAYPPFASKAPDGSIVGFDYDIGNALCEE
CCCHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCCCEEEEECCHHHHHHHH
MKVKCTWVEQEFDGLIPALKVRKIDAILSSMSITDDRKKSVDFTGKYYNSPARLVMKDGT
HEEEEEEECHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEECCCEEEEEECCC
TVSDSLAELKGKKIGVQRGTIHERFAREVLAPQGVEVTPYGSQNEIYLDISAGRLDGTIA
CHHHHHHHHCCCEECCCCCHHHHHHHHHHHCCCCEEEECCCCCCEEEEEEECCCCCCCHH
DATLLQDGFLKTDAGKGYAFVGPSFFDPKYFGDGIGIAVRKGDKANLDRLNAAIAAIRAN
HHHHHHCCCEEECCCCCEEEECCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHEEEECC
GKYKAIQDKYFDFDIFGK
CCEEEECCCCCCEEECCC
>Mature Secondary Structure
MKKLMLIGAMALSVLAQPIFADEKPLKIGIEAAYPPFASKAPDGSIVGFDYDIGNALCEE
CCCHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCCCEEEEECCHHHHHHHH
MKVKCTWVEQEFDGLIPALKVRKIDAILSSMSITDDRKKSVDFTGKYYNSPARLVMKDGT
HEEEEEEECHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEECCCEEEEEECCC
TVSDSLAELKGKKIGVQRGTIHERFAREVLAPQGVEVTPYGSQNEIYLDISAGRLDGTIA
CHHHHHHHHCCCEECCCCCHHHHHHHHHHHCCCCEEEECCCCCCEEEEEEECCCCCCCHH
DATLLQDGFLKTDAGKGYAFVGPSFFDPKYFGDGIGIAVRKGDKANLDRLNAAIAAIRAN
HHHHHHCCCEEECCCCCEEEECCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHEEEECC
GKYKAIQDKYFDFDIFGK
CCEEEECCCCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-ornithine [Periplasm]; H2O; ATP; L-arginine [Periplasm]; L-lysine [Periplasm] [C]

Specific reaction: ATP + L-ornithine [Periplasm] + H2O = ADP + phosphate + L-ornithine [Cytoplasm] ATP + L-arginine [Periplasm] + H2O = ADP + phosphate + L-arginine [Cytoplasm] ATP + L-lysine [Periplasm] + H2O = ADP + phosphate + L-lysine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503; 3040734; 9298646 [H]