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The map label for this gene is pucI [H]

Identifier: 66046760

GI number: 66046760

Start: 4207489

End: 4208940

Strand: Direct

Name: pucI [H]

Synonym: Psyr_3531

Alternate gene names: 66046760

Gene position: 4207489-4208940 (Clockwise)

Preceding gene: 66046757

Following gene: 66046761

Centisome position: 69.05

GC content: 60.74

Gene sequence:

>1452_bases
ATGACAGATCAACGCCCGGCCGGGTACAGCCCGCGCCTGCACAACCACGATCTGGGGCCGGTGCCGCAGAAGTGGACCTG
GTACAACATTCTGGCGTTCTGGATGAGCGATGTGCACAGCGTCGGCGGCTACGTTTTTGCCGCCAGTCTGTTCGCCCTGG
GCCTGGCCAGTTGGCAGGTGCTGATCGCCCTGCTGGTGGGGATCTGCATCATTCAGGTGATTGCCAACCTGGTCGCCAAA
CCCAGTCAGCAAGCCGCCGTGCCCTACCCGGTCATTTGCCGCCTGGCTTTCGGCGTGTTCGGTGCCAACATTCCCGCCAT
CATTCGCGGGCTGATCGCAGTGGCGTGGTACGGCATTCAAACCTACCTGGCGTCCAGCGCACTGGTGATCGTGGTGCTGC
GTTTCTTCCCGGAACTGGCCGTCTATCAAAGCGTGACATTTCTCGGACTGTCCTGGCTCGGCTGGTTCGGCTTTCTGGCG
TTGTGGGTGGTGCAAGCCGCGGTGTTCTGGACGGGTATGGAGTCGATCAGGCGCTTCATCGACTGGGCCGGCCCTGCCGT
TTATGCGGTGATGTTCGCTCTGGCAGGCTGGATTGTCTGGCGCGCCGGCTGGGACAACATCAGTTTTACTCTGGCTGAAA
AGGAGTTGTCCGGCTGGGCAGCGTTCGGCCAGGTGGTCATGGCCATTGCGCTGGTGGTCTCGTATTTCTCCGGGCCGACG
CTGAACTTCGGTGATTTCAGCCGCTATTGCCGCAGCATGGCCGACGTGCGCCGCGGCAATTTCTGGGGCTTACCGGTGAA
TTTTCTGGCGTTTTCACTGGTAACAGTGGTGATCGTCTCGGGCACGTTGCCGATTTTCGGTGAGATGATCCACGACCCGA
TTGCGACCGTCGCGCGCATCGACAACACCACGGCCGTGCTGCTCGGCGCCTTCACCTTCGTCACCGCCACGGTCGGCATC
AATATCGTCGCCAACTTCGTTTCCCCCGCCTTCGACTTCGCCAACGTGGCGCCCAGCCGCATCAGCTGGCGGGCAGGCGG
CATGATCGCAGCGGTAGCGTCGATCTTCATTACGCCCTGGAACCTGTTCAACAACCCTGAAGTGATTCACTACACCCTCG
ACGTGCTGGCGGCCTGTATCGGCCCACTGTTCGGCATCCTGCTGGTGGATTACTACCTGATCAAGAAGCAGCAGATCGAT
GTGGATGCCCTGTTCGATGACACGCCAAGCGGTCGCTACTGGTACACCAACGGCGTGAACTGGATCGCGGTCAAGGCATT
GCTGCTGACGGCACTGGTCGGTCTGTGCTTCACCTTTGTCGAGTGGTTCAAGCCGATTGCCAACTTCGCCTGGTTTACCG
GCTGCATCCTGGGCGGCGCAGTGTTTTACGCGTTGACCCGCTGGTCGCCCGTACAGGCTGCTAACCTTCCTGCGCCTGTC
GGCCAGCATTGA

Upstream 100 bases:

>100_bases
ACGAGAAATCGTATGGCACATGTTTTGCGTACACGATCGTATACAAAAAAATCATCGATCGTACTCCGATCCCGTCACGA
CACACCGAGGGAGTCACGCA

Downstream 100 bases:

>100_bases
GACGTCGCAAGACGCCAATACCTGAATGAAACCAGCGCTTTCAGAGGATTCGCCAAGCCCGGCATGACCTGAAAGCGCCG
CCCTGCCTGCTCCGCTGAAA

Product: permease for cytosine/purines, uracil, thiamine, allantoin

Products: NA

Alternate protein names: Allantoin transport protein [H]

Number of amino acids: Translated: 483; Mature: 482

Protein sequence:

>483_residues
MTDQRPAGYSPRLHNHDLGPVPQKWTWYNILAFWMSDVHSVGGYVFAASLFALGLASWQVLIALLVGICIIQVIANLVAK
PSQQAAVPYPVICRLAFGVFGANIPAIIRGLIAVAWYGIQTYLASSALVIVVLRFFPELAVYQSVTFLGLSWLGWFGFLA
LWVVQAAVFWTGMESIRRFIDWAGPAVYAVMFALAGWIVWRAGWDNISFTLAEKELSGWAAFGQVVMAIALVVSYFSGPT
LNFGDFSRYCRSMADVRRGNFWGLPVNFLAFSLVTVVIVSGTLPIFGEMIHDPIATVARIDNTTAVLLGAFTFVTATVGI
NIVANFVSPAFDFANVAPSRISWRAGGMIAAVASIFITPWNLFNNPEVIHYTLDVLAACIGPLFGILLVDYYLIKKQQID
VDALFDDTPSGRYWYTNGVNWIAVKALLLTALVGLCFTFVEWFKPIANFAWFTGCILGGAVFYALTRWSPVQAANLPAPV
GQH

Sequences:

>Translated_483_residues
MTDQRPAGYSPRLHNHDLGPVPQKWTWYNILAFWMSDVHSVGGYVFAASLFALGLASWQVLIALLVGICIIQVIANLVAK
PSQQAAVPYPVICRLAFGVFGANIPAIIRGLIAVAWYGIQTYLASSALVIVVLRFFPELAVYQSVTFLGLSWLGWFGFLA
LWVVQAAVFWTGMESIRRFIDWAGPAVYAVMFALAGWIVWRAGWDNISFTLAEKELSGWAAFGQVVMAIALVVSYFSGPT
LNFGDFSRYCRSMADVRRGNFWGLPVNFLAFSLVTVVIVSGTLPIFGEMIHDPIATVARIDNTTAVLLGAFTFVTATVGI
NIVANFVSPAFDFANVAPSRISWRAGGMIAAVASIFITPWNLFNNPEVIHYTLDVLAACIGPLFGILLVDYYLIKKQQID
VDALFDDTPSGRYWYTNGVNWIAVKALLLTALVGLCFTFVEWFKPIANFAWFTGCILGGAVFYALTRWSPVQAANLPAPV
GQH
>Mature_482_residues
TDQRPAGYSPRLHNHDLGPVPQKWTWYNILAFWMSDVHSVGGYVFAASLFALGLASWQVLIALLVGICIIQVIANLVAKP
SQQAAVPYPVICRLAFGVFGANIPAIIRGLIAVAWYGIQTYLASSALVIVVLRFFPELAVYQSVTFLGLSWLGWFGFLAL
WVVQAAVFWTGMESIRRFIDWAGPAVYAVMFALAGWIVWRAGWDNISFTLAEKELSGWAAFGQVVMAIALVVSYFSGPTL
NFGDFSRYCRSMADVRRGNFWGLPVNFLAFSLVTVVIVSGTLPIFGEMIHDPIATVARIDNTTAVLLGAFTFVTATVGIN
IVANFVSPAFDFANVAPSRISWRAGGMIAAVASIFITPWNLFNNPEVIHYTLDVLAACIGPLFGILLVDYYLIKKQQIDV
DALFDDTPSGRYWYTNGVNWIAVKALLLTALVGLCFTFVEWFKPIANFAWFTGCILGGAVFYALTRWSPVQAANLPAPVG
QH

Specific function: Transport of allantoin [H]

COG id: COG1953

COG function: function code FH; Cytosine/uracil/thiamine/allantoin permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purine-cytosine permease family [H]

Homologues:

Organism=Escherichia coli, GI87081749, Length=490, Percent_Identity=26.1224489795918, Blast_Score=203, Evalue=2e-53,
Organism=Saccharomyces cerevisiae, GI6319495, Length=436, Percent_Identity=23.8532110091743, Blast_Score=130, Evalue=3e-31,
Organism=Saccharomyces cerevisiae, GI6319429, Length=446, Percent_Identity=24.4394618834081, Blast_Score=128, Evalue=2e-30,
Organism=Saccharomyces cerevisiae, GI6322219, Length=436, Percent_Identity=22.4770642201835, Blast_Score=119, Evalue=1e-27,
Organism=Saccharomyces cerevisiae, GI6324766, Length=427, Percent_Identity=20.8430913348946, Blast_Score=91, Evalue=3e-19,
Organism=Saccharomyces cerevisiae, GI6323266, Length=442, Percent_Identity=22.3981900452489, Blast_Score=89, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6324645, Length=433, Percent_Identity=21.4780600461894, Blast_Score=85, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001248
- InterPro:   IPR012681 [H]

Pfam domain/function: PF02133 Transp_cyt_pur [H]

EC number: NA

Molecular weight: Translated: 53032; Mature: 52901

Theoretical pI: Translated: 7.82; Mature: 7.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCC
>Mature Secondary Structure 
TDQRPAGYSPRLHNHDLGPVPQKWTWYNILAFWMSDVHSVGGYVFAASLFALGLASWQV
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH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CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9353933; 9384377; 11344136 [H]