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The map label for this gene is dsbE [H]

Identifier: 66046616

GI number: 66046616

Start: 4046088

End: 4046624

Strand: Reverse

Name: dsbE [H]

Synonym: Psyr_3387

Alternate gene names: 66046616

Gene position: 4046624-4046088 (Counterclockwise)

Preceding gene: 66046617

Following gene: 66046615

Centisome position: 66.41

GC content: 58.85

Gene sequence:

>537_bases
ATGAAACGTTGGGTCATGGTACTGCCACTGGTGCTGTTTCTGGGCGTCGCGGCGTTCTTGTATCGCGGTTTGTATCTGGA
CCCCGCCGAGCTGCCCTCGGCACTGATCGGCAAGCCTTTTCCGGCATTTTCGCTACCAGCGGTGCAGGGCGAGCGGATGT
TGAGCCGTGCCGATCTGCTCGGCAAACCTGCGCTGGTCAACGTTTGGGGCACCTGGTGCGTGGCCTGCCGGGTGGAGCAT
CCGGTGCTCACCCGGCTGGCGCAGCAGGGCGTGCTGATCTATGGCGTGAATTACAAGGACGTCAACGCCGACGCGCTGAA
ATGGCTTAAGGAATTTCATGATCCGTATCAGTTGAATATTCGTGATCAGGACGGCTCTCTGGGGCTCAATCTGGGGGTGT
ACGGTGCGCCGGAAACCTTCCTGATCGACGCTCAAGGCATCATTCGTTACAAACACGTCGGGGTCATCGACGACGCAGTT
TGGCGCGAAAAGCTTGCCGCGCGCTATCAGCAACTGGTTGATGAGGCACAGCCATGA

Upstream 100 bases:

>100_bases
TGACCGGTCTGGGCGGGGTGCTGGCGGCGCTGGACCGGCGTTATCGCACCCGGGTGAAGAACAAGGTGCGCGAGGCGTTG
GGTATGTCGGGAGCTGCACA

Downstream 100 bases:

>100_bases
AGCGCTGTCTGTTGATCGTCCTGCTCAGCGTGCTGTGCTCAGGCATTGCTCAAGCGGCCATCGACGCTTATGCCTTTCGC
GACGACGCCGAGCGCGCGCG

Product: periplasmic protein thiol:disulfide oxidoreductase DsbE

Products: NA

Alternate protein names: Cytochrome c biogenesis protein CcmG [H]

Number of amino acids: Translated: 178; Mature: 178

Protein sequence:

>178_residues
MKRWVMVLPLVLFLGVAAFLYRGLYLDPAELPSALIGKPFPAFSLPAVQGERMLSRADLLGKPALVNVWGTWCVACRVEH
PVLTRLAQQGVLIYGVNYKDVNADALKWLKEFHDPYQLNIRDQDGSLGLNLGVYGAPETFLIDAQGIIRYKHVGVIDDAV
WREKLAARYQQLVDEAQP

Sequences:

>Translated_178_residues
MKRWVMVLPLVLFLGVAAFLYRGLYLDPAELPSALIGKPFPAFSLPAVQGERMLSRADLLGKPALVNVWGTWCVACRVEH
PVLTRLAQQGVLIYGVNYKDVNADALKWLKEFHDPYQLNIRDQDGSLGLNLGVYGAPETFLIDAQGIIRYKHVGVIDDAV
WREKLAARYQQLVDEAQP
>Mature_178_residues
MKRWVMVLPLVLFLGVAAFLYRGLYLDPAELPSALIGKPFPAFSLPAVQGERMLSRADLLGKPALVNVWGTWCVACRVEH
PVLTRLAQQGVLIYGVNYKDVNADALKWLKEFHDPYQLNIRDQDGSLGLNLGVYGAPETFLIDAQGIIRYKHVGVIDDAV
WREKLAARYQQLVDEAQP

Specific function: Involved in disulfide bond formation. Catalyzes a late, reductive step in the assembly of periplasmic c-type cytochromes, probably the reduction of disulfide bonds of the apocytochrome c to allow covalent linkage with the heme. Possible subunit of a heme

COG id: COG0526

COG function: function code OC; Thiol-disulfide isomerase and thioredoxins

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein; Periplasmic side [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 thioredoxin domain [H]

Homologues:

Organism=Escherichia coli, GI1788523, Length=183, Percent_Identity=49.1803278688525, Blast_Score=177, Evalue=4e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004799
- InterPro:   IPR013740
- InterPro:   IPR005746
- InterPro:   IPR017936
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF08534 Redoxin [H]

EC number: NA

Molecular weight: Translated: 19947; Mature: 19947

Theoretical pI: Translated: 7.50; Mature: 7.50

Prosite motif: PS00194 THIOREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRWVMVLPLVLFLGVAAFLYRGLYLDPAELPSALIGKPFPAFSLPAVQGERMLSRADLL
CCCHHHHHHHHHHHHHHHHHHCCCCCCHHHCCHHHHCCCCCCCCCCCCCHHHHHHHHHHC
GKPALVNVWGTWCVACRVEHPVLTRLAQQGVLIYGVNYKDVNADALKWLKEFHDPYQLNI
CCCCEEEHHHHHHEEEECCCHHHHHHHHCCEEEEECCCCCCCHHHHHHHHHHCCCEEEEE
RDQDGSLGLNLGVYGAPETFLIDAQGIIRYKHVGVIDDAVWREKLAARYQQLVDEAQP
ECCCCCEEEEEEECCCCCEEEEECCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKRWVMVLPLVLFLGVAAFLYRGLYLDPAELPSALIGKPFPAFSLPAVQGERMLSRADLL
CCCHHHHHHHHHHHHHHHHHHCCCCCCHHHCCHHHHCCCCCCCCCCCCCHHHHHHHHHHC
GKPALVNVWGTWCVACRVEHPVLTRLAQQGVLIYGVNYKDVNADALKWLKEFHDPYQLNI
CCCCEEEHHHHHHEEEECCCHHHHHHHHCCEEEEECCCCCCCHHHHHHHHHHCCCEEEEE
RDQDGSLGLNLGVYGAPETFLIDAQGIIRYKHVGVIDDAVWREKLAARYQQLVDEAQP
ECCCCCEEEEEEECCCCCEEEEECCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8878040; 8692990 [H]