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The map label for this gene is glcF [H]

Identifier: 66046562

GI number: 66046562

Start: 3988305

End: 3989537

Strand: Direct

Name: glcF [H]

Synonym: Psyr_3333

Alternate gene names: 66046562

Gene position: 3988305-3989537 (Clockwise)

Preceding gene: 66046561

Following gene: 66046563

Centisome position: 65.45

GC content: 64.15

Gene sequence:

>1233_bases
ATGCAGACCACATTAAGTGAACACGCCAGAACCCTGCCGCGCGCCGAAGAAGCCGAACGTATCCTGCGCAGTTGTGTGCA
TTGCGGTTTCTGCAACGCCACCTGTCCGACCTATCAATTGCTGGGGGATGAGCTGGACGGTCCACGCGGCAGGATCTACC
TGATCAAGCAGGTGCTAGAAGGTCAGCCGGTCACCGAGAAAACCCGTCTGCATCTGGACCGCTGCCTGAGCTGTCGCAAC
TGCGAAACCACCTGCCCGTCCGGCGTGGATTACCACAACCTGCTGGACATCGGCCGCGCGGCGGTTGAAGACCTGGCCCC
GCGGCCAATCGCCGAACGCATGCTGCGTGAAGGCTTGCGTCGGGTCATCCCCCACCCCGGACTGTTCAGATCCCTGACCG
GCATGGGCCTGAAGTTCAGGCCATTGCTGCCACAGACCCTGGCCAGCAAGCTGCCGACGACGGTACACCCTGCCGGCCTT
CGCCCGCACACCGGTCATGCGCGCCAGGTGCTGCTGCTGGAAGGTTGCGTTCAGCCGGGCCTGTCGCCCAACACCAACAG
CGCCACGGCGCGGGTGCTGGATCGTCTGGGCATCGGCATTATGGCGGTCAGCCAGGCGGGTTGCTGCGGCGCCGTGGATT
ATCACCTCAACGCACAGCAGGCCGGGCTGGACCGGGCGCGGCGCAACATCGATGCGTGGTGGCCTGCGCTACAGGGCGGT
GCTGAAAGCCTGATCCAGACGGCCAGTGGCTGCGGAGCCTTCGTCAAGGACTACGGCCACCTGCTGCGCCATGATCCAGG
CTACGCCGACAAGGCCGAGCAGATCAGCGCGCGAACAAAGGATCTGGTCGAACTGCTGCGCGACGAGCCCCTGGAAACCC
TCGGCATAAAGGCCGAGACGCGCCTGGCGTTTCACTGCCCGTGTACGCTGCAACACGCGCAAAAACTCGGCGGCGAAGTC
GAGCGCGTCCTGACTCGTCTAGGCTTCAATCTGACCACAGTGGCGGACGGTCATTTATGCTGCGGCTCTGCAGGCACCTA
CTCCATCACCCAGCCTGCCATCGCCCGCCAGTTGCGCGACAACCGCATGAACGCGCTGGAGAGCGGCAAGCCGCAGGTCA
TCGCCACCGCCAATATCGGTTGTCAGACCCATCTGGCCAGCGCCGGCCGAACGCCAGTGCGGCACTGGATCGAACTGATC
GACGCGGCACTTGGCACGCCTGAATCACACTGA

Upstream 100 bases:

>100_bases
GTTGGCCGCACCTGTGCTGCGCTACCATCAGGCACTCAAGACCCGACTCGACCCGCAGGGGATTTTCAACCCCGGCCGAC
TGTACGCGGAGCTTTGAAAC

Downstream 100 bases:

>100_bases
ACCCTATTCAATAATCACTGCTCCGGAGAACCCCATGAAGATCAAAGCCGTCCTGACCCAGACCGAAGTCAGCCTGATGC
TCGGCACCGCACGCGAAGAG

Product: glycolate oxidase iron-sulfur subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 410; Mature: 410

Protein sequence:

>410_residues
MQTTLSEHARTLPRAEEAERILRSCVHCGFCNATCPTYQLLGDELDGPRGRIYLIKQVLEGQPVTEKTRLHLDRCLSCRN
CETTCPSGVDYHNLLDIGRAAVEDLAPRPIAERMLREGLRRVIPHPGLFRSLTGMGLKFRPLLPQTLASKLPTTVHPAGL
RPHTGHARQVLLLEGCVQPGLSPNTNSATARVLDRLGIGIMAVSQAGCCGAVDYHLNAQQAGLDRARRNIDAWWPALQGG
AESLIQTASGCGAFVKDYGHLLRHDPGYADKAEQISARTKDLVELLRDEPLETLGIKAETRLAFHCPCTLQHAQKLGGEV
ERVLTRLGFNLTTVADGHLCCGSAGTYSITQPAIARQLRDNRMNALESGKPQVIATANIGCQTHLASAGRTPVRHWIELI
DAALGTPESH

Sequences:

>Translated_410_residues
MQTTLSEHARTLPRAEEAERILRSCVHCGFCNATCPTYQLLGDELDGPRGRIYLIKQVLEGQPVTEKTRLHLDRCLSCRN
CETTCPSGVDYHNLLDIGRAAVEDLAPRPIAERMLREGLRRVIPHPGLFRSLTGMGLKFRPLLPQTLASKLPTTVHPAGL
RPHTGHARQVLLLEGCVQPGLSPNTNSATARVLDRLGIGIMAVSQAGCCGAVDYHLNAQQAGLDRARRNIDAWWPALQGG
AESLIQTASGCGAFVKDYGHLLRHDPGYADKAEQISARTKDLVELLRDEPLETLGIKAETRLAFHCPCTLQHAQKLGGEV
ERVLTRLGFNLTTVADGHLCCGSAGTYSITQPAIARQLRDNRMNALESGKPQVIATANIGCQTHLASAGRTPVRHWIELI
DAALGTPESH
>Mature_410_residues
MQTTLSEHARTLPRAEEAERILRSCVHCGFCNATCPTYQLLGDELDGPRGRIYLIKQVLEGQPVTEKTRLHLDRCLSCRN
CETTCPSGVDYHNLLDIGRAAVEDLAPRPIAERMLREGLRRVIPHPGLFRSLTGMGLKFRPLLPQTLASKLPTTVHPAGL
RPHTGHARQVLLLEGCVQPGLSPNTNSATARVLDRLGIGIMAVSQAGCCGAVDYHLNAQQAGLDRARRNIDAWWPALQGG
AESLIQTASGCGAFVKDYGHLLRHDPGYADKAEQISARTKDLVELLRDEPLETLGIKAETRLAFHCPCTLQHAQKLGGEV
ERVLTRLGFNLTTVADGHLCCGSAGTYSITQPAIARQLRDNRMNALESGKPQVIATANIGCQTHLASAGRTPVRHWIELI
DAALGTPESH

Specific function: Unknown

COG id: COG0247

COG function: function code C; Fe-S oxidoreductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI48994913, Length=404, Percent_Identity=70.7920792079208, Blast_Score=605, Evalue=1e-174,
Organism=Escherichia coli, GI1786497, Length=216, Percent_Identity=25, Blast_Score=72, Evalue=6e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR004017
- InterPro:   IPR012285
- InterPro:   IPR012257 [H]

Pfam domain/function: PF02754 CCG [H]

EC number: NA

Molecular weight: Translated: 44610; Mature: 44610

Theoretical pI: Translated: 8.02; Mature: 8.02

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.1 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
4.1 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQTTLSEHARTLPRAEEAERILRSCVHCGFCNATCPTYQLLGDELDGPRGRIYLIKQVLE
CCCCHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCCCCCCCHHHHHHHHHC
GQPVTEKTRLHLDRCLSCRNCETTCPSGVDYHNLLDIGRAAVEDLAPRPIAERMLREGLR
CCCCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
RVIPHPGLFRSLTGMGLKFRPLLPQTLASKLPTTVHPAGLRPHTGHARQVLLLEGCVQPG
HHCCCCCHHHHHHCCCCEECCCCHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHCCC
LSPNTNSATARVLDRLGIGIMAVSQAGCCGAVDYHLNAQQAGLDRARRNIDAWWPALQGG
CCCCCCHHHHHHHHHHCCCCEEECCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHH
AESLIQTASGCGAFVKDYGHLLRHDPGYADKAEQISARTKDLVELLRDEPLETLGIKAET
HHHHHHHHHCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHCCCCCC
RLAFHCPCTLQHAQKLGGEVERVLTRLGFNLTTVADGHLCCGSAGTYSITQPAIARQLRD
EEEEECCCHHHHHHHHCHHHHHHHHHHCCCEEEECCCCEEECCCCCCCCCHHHHHHHHHH
NRMNALESGKPQVIATANIGCQTHLASAGRTPVRHWIELIDAALGTPESH
HHHHHHHCCCCCEEEEECCCCHHHHHHCCCCHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MQTTLSEHARTLPRAEEAERILRSCVHCGFCNATCPTYQLLGDELDGPRGRIYLIKQVLE
CCCCHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCCCCCCCHHHHHHHHHC
GQPVTEKTRLHLDRCLSCRNCETTCPSGVDYHNLLDIGRAAVEDLAPRPIAERMLREGLR
CCCCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
RVIPHPGLFRSLTGMGLKFRPLLPQTLASKLPTTVHPAGLRPHTGHARQVLLLEGCVQPG
HHCCCCCHHHHHHCCCCEECCCCHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHCCC
LSPNTNSATARVLDRLGIGIMAVSQAGCCGAVDYHLNAQQAGLDRARRNIDAWWPALQGG
CCCCCCHHHHHHHHHHCCCCEEECCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHH
AESLIQTASGCGAFVKDYGHLLRHDPGYADKAEQISARTKDLVELLRDEPLETLGIKAET
HHHHHHHHHCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHCCCCCC
RLAFHCPCTLQHAQKLGGEVERVLTRLGFNLTTVADGHLCCGSAGTYSITQPAIARQLRD
EEEEECCCHHHHHHHHCHHHHHHHHHHCCCEEEECCCCEEECCCCCCCCCHHHHHHHHHH
NRMNALESGKPQVIATANIGCQTHLASAGRTPVRHWIELIDAALGTPESH
HHHHHHHCCCCCEEEEECCCCHHHHHHCCCCHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8606183; 9278503 [H]