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The map label for this gene is glcD [H]

Identifier: 66046560

GI number: 66046560

Start: 3985744

End: 3987243

Strand: Direct

Name: glcD [H]

Synonym: Psyr_3331

Alternate gene names: 66046560

Gene position: 3985744-3987243 (Clockwise)

Preceding gene: 66046558

Following gene: 66046561

Centisome position: 65.41

GC content: 63.87

Gene sequence:

>1500_bases
ATGAACATTCTGTACGACGAACGCATCGACGGCGTGCTGCCTGCCGTCGACAAGCAGCAACTGTTGCAGGCGCTGCAACA
GCAGTTGCCCGACCTGGACATCCTGCATCGCCCCGAAGAGCTGCGGCCTTATGAGTGCGACGGGTTGTCAGCCTATCGCA
CCACACCGATGCTGGTGGCGTTGCCGCGTCATGTGGAAGAAGTCCAGACGCTGCTCAGGCTGTGCCACGCACGGCGCGTA
CCGGTGGTCGCTCGTGGGGCGGGCACCGGGCTGTCGGGTGGCGCGCTGCCGCTGGAAAAAGGCGTGCTGCTGGTCATGGC
GCGCTTCAATCAGATCCTCGAGATCGACCCTTCGGCGCGCCTGGCGCGGGTTCAGCCCGGCGTGCGCAACCTGGCCATTT
CCCAGGCGGCAGCGCCCCACGGCCTGTATTACGCGCCAGACCCGTCCTCGCAAATCGCCTGCTCCATCGGCGGCAATGTG
GCGGAAAATGCCGGCGGCGTGCACTGCCTCAAGTACGGGCTGACCGTGCATAACCTGCTCAAGGTCGAGATCCTGACGGT
GGAAGGCGAGCCCATGACGCTGGGCAGCGACACGCTGGACTCGCCGGGCTTCGACCTGCTGGCACTGTTCACCGGCTCCG
AAGGCATGCTCGGCGTCATTACCGAAGTCACCGTCAAGTTGTTGCCCAGACCGCAAGTGGCCAAAGTACTGCTGGCAGCG
TTCGATTCCGTGGAAAAGGCCGGACGTGCAGTGGCCGATATCATTGCGGCGGGCATCATCCCCGGCGGGCTGGAGATGAT
GGACAATCTGGCGATTCGTGCCGCCGAGGATTTCATTCACGCAGGCTACCCGGTCGATGCCGAAGCCATCCTGCTCTGCG
AACTGGACGGCGTCGAAGCCGATGTGCACGCTGACTGCGAACGGGTCAGCGAGGTCTTGACACGGGCGGGCGCCACCGAC
GTGCGCCAGGCGAAAGACGAGGCCGAACGGGTCCGCTTCTGGGCCGGGCGCAAAAACGCTTTCCCGGCCGTGGGTCGCCT
GTCGCCGGATTACTACTGCATGGACGGGACCATCCCGCGCCGTGAGCTGCCGGGCGTATTGCGCAGCATCAGGGAGTTAT
CCGGGCAATACGGCTTGCGTGTCGCCAACGTGTTTCATGCCGGTGACGGCAACATGCACCCGCTGATCCTGTTCGACGCC
AATCAACCGGGTGAGCTGGAGCGCGCCGAGGCACTGGGCGGCAAAATCCTTGAGCTGTGCGTCAAGGTTGGCGGCAGCAT
CACCGGCGAGCACGGTGTCGGTCGCGAGAAGATCAATCAGATGTGCGCCCAGTTCAATAGCGACGAGCTGACCTTCTTCC
ATGCCATCAAGGCGGCATTCGACGCCAGCGGTCTGCTCAACCCTGGCAAGAACATCCCGACCCTGCACCGTTGCGCGGAA
TTTGGTGCCATGCACATCCACGCCGGGCAATTGCCGTTCCCCGACCTGGAGCGCTTCTGA

Upstream 100 bases:

>100_bases
AATGCGTAAATTGCGTGATCGGACCCTCGCTGTCAACTCAAGCCTCGCAGCGAATGAGCTAGGATGCATTAACCCGACAA
TAACAACCCAGGGTGGCTTG

Downstream 100 bases:

>100_bases
TGAGCACGATGGACAACGATGTCAGCCAGGCACTGCTCGAGCGGGTCGAACAGGCGCTGCGCGACAACACGCCGTTACGT
CTTCAGGGCGGTAACAGCAA

Product: glycolate oxidase subunit GlcD

Products: 2-oxo acid; H2O2

Alternate protein names: NA

Number of amino acids: Translated: 499; Mature: 499

Protein sequence:

>499_residues
MNILYDERIDGVLPAVDKQQLLQALQQQLPDLDILHRPEELRPYECDGLSAYRTTPMLVALPRHVEEVQTLLRLCHARRV
PVVARGAGTGLSGGALPLEKGVLLVMARFNQILEIDPSARLARVQPGVRNLAISQAAAPHGLYYAPDPSSQIACSIGGNV
AENAGGVHCLKYGLTVHNLLKVEILTVEGEPMTLGSDTLDSPGFDLLALFTGSEGMLGVITEVTVKLLPRPQVAKVLLAA
FDSVEKAGRAVADIIAAGIIPGGLEMMDNLAIRAAEDFIHAGYPVDAEAILLCELDGVEADVHADCERVSEVLTRAGATD
VRQAKDEAERVRFWAGRKNAFPAVGRLSPDYYCMDGTIPRRELPGVLRSIRELSGQYGLRVANVFHAGDGNMHPLILFDA
NQPGELERAEALGGKILELCVKVGGSITGEHGVGREKINQMCAQFNSDELTFFHAIKAAFDASGLLNPGKNIPTLHRCAE
FGAMHIHAGQLPFPDLERF

Sequences:

>Translated_499_residues
MNILYDERIDGVLPAVDKQQLLQALQQQLPDLDILHRPEELRPYECDGLSAYRTTPMLVALPRHVEEVQTLLRLCHARRV
PVVARGAGTGLSGGALPLEKGVLLVMARFNQILEIDPSARLARVQPGVRNLAISQAAAPHGLYYAPDPSSQIACSIGGNV
AENAGGVHCLKYGLTVHNLLKVEILTVEGEPMTLGSDTLDSPGFDLLALFTGSEGMLGVITEVTVKLLPRPQVAKVLLAA
FDSVEKAGRAVADIIAAGIIPGGLEMMDNLAIRAAEDFIHAGYPVDAEAILLCELDGVEADVHADCERVSEVLTRAGATD
VRQAKDEAERVRFWAGRKNAFPAVGRLSPDYYCMDGTIPRRELPGVLRSIRELSGQYGLRVANVFHAGDGNMHPLILFDA
NQPGELERAEALGGKILELCVKVGGSITGEHGVGREKINQMCAQFNSDELTFFHAIKAAFDASGLLNPGKNIPTLHRCAE
FGAMHIHAGQLPFPDLERF
>Mature_499_residues
MNILYDERIDGVLPAVDKQQLLQALQQQLPDLDILHRPEELRPYECDGLSAYRTTPMLVALPRHVEEVQTLLRLCHARRV
PVVARGAGTGLSGGALPLEKGVLLVMARFNQILEIDPSARLARVQPGVRNLAISQAAAPHGLYYAPDPSSQIACSIGGNV
AENAGGVHCLKYGLTVHNLLKVEILTVEGEPMTLGSDTLDSPGFDLLALFTGSEGMLGVITEVTVKLLPRPQVAKVLLAA
FDSVEKAGRAVADIIAAGIIPGGLEMMDNLAIRAAEDFIHAGYPVDAEAILLCELDGVEADVHADCERVSEVLTRAGATD
VRQAKDEAERVRFWAGRKNAFPAVGRLSPDYYCMDGTIPRRELPGVLRSIRELSGQYGLRVANVFHAGDGNMHPLILFDA
NQPGELERAEALGGKILELCVKVGGSITGEHGVGREKINQMCAQFNSDELTFFHAIKAAFDASGLLNPGKNIPTLHRCAE
FGAMHIHAGQLPFPDLERF

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding PCMH-type domain [H]

Homologues:

Organism=Homo sapiens, GI37595756, Length=421, Percent_Identity=30.4038004750594, Blast_Score=196, Evalue=3e-50,
Organism=Homo sapiens, GI37595754, Length=444, Percent_Identity=29.0540540540541, Blast_Score=189, Evalue=6e-48,
Organism=Homo sapiens, GI119964728, Length=466, Percent_Identity=29.3991416309013, Blast_Score=172, Evalue=8e-43,
Organism=Homo sapiens, GI4501993, Length=252, Percent_Identity=25.7936507936508, Blast_Score=78, Evalue=2e-14,
Organism=Escherichia coli, GI1789351, Length=499, Percent_Identity=80.9619238476954, Blast_Score=840, Evalue=0.0,
Organism=Escherichia coli, GI1787977, Length=501, Percent_Identity=26.1477045908184, Blast_Score=122, Evalue=4e-29,
Organism=Escherichia coli, GI48994907, Length=487, Percent_Identity=21.7659137577002, Blast_Score=74, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI71992373, Length=476, Percent_Identity=27.9411764705882, Blast_Score=189, Evalue=2e-48,
Organism=Caenorhabditis elegans, GI71992381, Length=475, Percent_Identity=26.5263157894737, Blast_Score=169, Evalue=2e-42,
Organism=Caenorhabditis elegans, GI71992392, Length=371, Percent_Identity=27.2237196765499, Blast_Score=151, Evalue=9e-37,
Organism=Caenorhabditis elegans, GI17534361, Length=452, Percent_Identity=26.9911504424779, Blast_Score=150, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI17556096, Length=454, Percent_Identity=21.3656387665198, Blast_Score=79, Evalue=5e-15,
Organism=Saccharomyces cerevisiae, GI6320023, Length=479, Percent_Identity=27.5574112734864, Blast_Score=169, Evalue=1e-42,
Organism=Saccharomyces cerevisiae, GI6320027, Length=435, Percent_Identity=27.1264367816092, Blast_Score=164, Evalue=3e-41,
Organism=Saccharomyces cerevisiae, GI6320764, Length=459, Percent_Identity=26.3616557734205, Blast_Score=161, Evalue=2e-40,
Organism=Drosophila melanogaster, GI18921117, Length=448, Percent_Identity=28.7946428571429, Blast_Score=156, Evalue=3e-38,
Organism=Drosophila melanogaster, GI24639277, Length=448, Percent_Identity=28.7946428571429, Blast_Score=156, Evalue=3e-38,
Organism=Drosophila melanogaster, GI24639275, Length=448, Percent_Identity=28.7946428571429, Blast_Score=156, Evalue=3e-38,
Organism=Drosophila melanogaster, GI24653753, Length=222, Percent_Identity=27.9279279279279, Blast_Score=79, Evalue=9e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016166
- InterPro:   IPR016167
- InterPro:   IPR016164
- InterPro:   IPR016168
- InterPro:   IPR004113
- InterPro:   IPR004490
- InterPro:   IPR006094
- InterPro:   IPR016171 [H]

Pfam domain/function: PF02913 FAD-oxidase_C; PF01565 FAD_binding_4 [H]

EC number: 1.1.3.15

Molecular weight: Translated: 53822; Mature: 53822

Theoretical pI: Translated: 5.33; Mature: 5.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNILYDERIDGVLPAVDKQQLLQALQQQLPDLDILHRPEELRPYECDGLSAYRTTPMLVA
CCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHCCCHHCCCCCCCCCCHHCCCCEEEE
LPRHVEEVQTLLRLCHARRVPVVARGAGTGLSGGALPLEKGVLLVMARFNQILEIDPSAR
CHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCHHCCHHHHHHHHHHHEEECCCCH
LARVQPGVRNLAISQAAAPHGLYYAPDPSSQIACSIGGNVAENAGGVHCLKYGLTVHNLL
HHHCCCCHHHHHHHHCCCCCCCEECCCCCCCEEEEECCCHHCCCCCEEEEHHCCHHHHEE
KVEILTVEGEPMTLGSDTLDSPGFDLLALFTGSEGMLGVITEVTVKLLPRPQVAKVLLAA
EEEEEEECCCCEECCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHCCCHHHHHHHHHH
FDSVEKAGRAVADIIAAGIIPGGLEMMDNLAIRAAEDFIHAGYPVDAEAILLCELDGVEA
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCC
DVHADCERVSEVLTRAGATDVRQAKDEAERVRFWAGRKNAFPAVGRLSPDYYCMDGTIPR
CHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEECCCCCH
RELPGVLRSIRELSGQYGLRVANVFHAGDGNMHPLILFDANQPGELERAEALGGKILELC
HHHHHHHHHHHHHHHHCCEEEEEEEECCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHH
VKVGGSITGEHGVGREKINQMCAQFNSDELTFFHAIKAAFDASGLLNPGKNIPTLHRCAE
HHHCCCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHH
FGAMHIHAGQLPFPDLERF
HCCEEEECCCCCCCHHCCC
>Mature Secondary Structure
MNILYDERIDGVLPAVDKQQLLQALQQQLPDLDILHRPEELRPYECDGLSAYRTTPMLVA
CCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHCCCHHCCCCCCCCCCHHCCCCEEEE
LPRHVEEVQTLLRLCHARRVPVVARGAGTGLSGGALPLEKGVLLVMARFNQILEIDPSAR
CHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCHHCCHHHHHHHHHHHEEECCCCH
LARVQPGVRNLAISQAAAPHGLYYAPDPSSQIACSIGGNVAENAGGVHCLKYGLTVHNLL
HHHCCCCHHHHHHHHCCCCCCCEECCCCCCCEEEEECCCHHCCCCCEEEEHHCCHHHHEE
KVEILTVEGEPMTLGSDTLDSPGFDLLALFTGSEGMLGVITEVTVKLLPRPQVAKVLLAA
EEEEEEECCCCEECCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHCCCHHHHHHHHHH
FDSVEKAGRAVADIIAAGIIPGGLEMMDNLAIRAAEDFIHAGYPVDAEAILLCELDGVEA
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCC
DVHADCERVSEVLTRAGATDVRQAKDEAERVRFWAGRKNAFPAVGRLSPDYYCMDGTIPR
CHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEECCCCCH
RELPGVLRSIRELSGQYGLRVANVFHAGDGNMHPLILFDANQPGELERAEALGGKILELC
HHHHHHHHHHHHHHHHCCEEEEEEEECCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHH
VKVGGSITGEHGVGREKINQMCAQFNSDELTFFHAIKAAFDASGLLNPGKNIPTLHRCAE
HHHCCCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHH
FGAMHIHAGQLPFPDLERF
HCCEEEECCCCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (S)-2-hydroxy acid; O2

Specific reaction: (S)-2-hydroxy acid + O2 = 2-oxo acid + H2O2

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]