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The map label for this gene is yeiE [H]

Identifier: 66046541

GI number: 66046541

Start: 3964004

End: 3964957

Strand: Reverse

Name: yeiE [H]

Synonym: Psyr_3312

Alternate gene names: 66046541

Gene position: 3964957-3964004 (Counterclockwise)

Preceding gene: 66046550

Following gene: 66046529

Centisome position: 65.07

GC content: 62.26

Gene sequence:

>954_bases
TTGAGAATCAACCTGCGGCAATTACAGATTTTTTGCGCGATCACCCGTTGTGGCAGCACTACCAGCGCAGGCAGCGCTCT
GGCGCTGTCCCAGTCAGCCACCAGCGCGGCATTGAACGAGCTGGAAAGCGCGTTGGGTACGCGTTTGTTCGACCGCGTGG
GCAAGCGGCTGGTGCTCAATGACAGTGGGCAGGCATTGCTGCCGCGTGCCATGAAGATGCTGGAAAACGCCCAGCAGATT
GAGGACAGCTTTCTGTTGCCCGACGCGGCGTTGAATGCTCGCCTGCGTATCGGCAGTAGCAGCACCATCGGCAATTACGT
TGTACCGCAGATCGTCGGGGCACTGCGCGGCTCGACGCCTCAATTGCGCATCGATGTGGACATCGGCAACAGCGCGCTGA
TTGCTCGCAAGGTCGCGCAGTTTGAAATCGACATGGGGCTGATCGAGGCACCCTGTCATCTGCCGGAATTGATCGCCGAG
CCATGGCTGGTGGATGAAATGGTCATCGTTGCCGGTCGCGCCCATCCGCTGGCCGGGGAGGAACACGTGTCACTGGGCGA
ACTGCAGAGCGCGGAGTGGCTGCTGCGTGAGCAGGGTTCGGGGACGCGCGAAGAGGTCGAACACTTGCTGCTCAGGCATT
TGCATGACCTGCAGGACATGCGTCAGGTAGGCAGCTCGGAAGCAATCATGCGCACCCTCGCGCAAGGCATCGGCATCAGT
TGCCTGTCCCGTCGTGTGGTCGCCGACCTGCTGGCCAGCGGGCAGTTGCGCACGCTCGCCAGCCCGCTGCCGCCGCTCAA
TCGCCGGTTCTTCATGATCAGGCACCGCGACAAGTTCATCTCGCCGAGCCTGGAACGCTTCAGGGAAGCCTGCCGCCAGG
CGGCCGATCAGGCCGATACGTTACGCCACGGTTTGAGCCATAACGCCAGCCCGACCAGCAGCAGCGCGCCGTAA

Upstream 100 bases:

>100_bases
TGCCATCAGTCTACGGATCGACAGTCAATCTTCTAAACGGGTAATCTCTCTCATCGCTACCTGTTTATCAGGTAGATTTA
TTCGGCGAGAGAAGGCGCTC

Downstream 100 bases:

>100_bases
ACACAGCTCAAGCCCAGTTGCAGCCAGGTGTCGTGCAATGGATGCAGCGACAGGGCGGCGATAGCCATCAGCAGGCTGGC
CAGCAACCAGTGGCTGCGCC

Product: regulatory protein, LysR:LysR, substrate-binding

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 317; Mature: 317

Protein sequence:

>317_residues
MRINLRQLQIFCAITRCGSTTSAGSALALSQSATSAALNELESALGTRLFDRVGKRLVLNDSGQALLPRAMKMLENAQQI
EDSFLLPDAALNARLRIGSSSTIGNYVVPQIVGALRGSTPQLRIDVDIGNSALIARKVAQFEIDMGLIEAPCHLPELIAE
PWLVDEMVIVAGRAHPLAGEEHVSLGELQSAEWLLREQGSGTREEVEHLLLRHLHDLQDMRQVGSSEAIMRTLAQGIGIS
CLSRRVVADLLASGQLRTLASPLPPLNRRFFMIRHRDKFISPSLERFREACRQAADQADTLRHGLSHNASPTSSSAP

Sequences:

>Translated_317_residues
MRINLRQLQIFCAITRCGSTTSAGSALALSQSATSAALNELESALGTRLFDRVGKRLVLNDSGQALLPRAMKMLENAQQI
EDSFLLPDAALNARLRIGSSSTIGNYVVPQIVGALRGSTPQLRIDVDIGNSALIARKVAQFEIDMGLIEAPCHLPELIAE
PWLVDEMVIVAGRAHPLAGEEHVSLGELQSAEWLLREQGSGTREEVEHLLLRHLHDLQDMRQVGSSEAIMRTLAQGIGIS
CLSRRVVADLLASGQLRTLASPLPPLNRRFFMIRHRDKFISPSLERFREACRQAADQADTLRHGLSHNASPTSSSAP
>Mature_317_residues
MRINLRQLQIFCAITRCGSTTSAGSALALSQSATSAALNELESALGTRLFDRVGKRLVLNDSGQALLPRAMKMLENAQQI
EDSFLLPDAALNARLRIGSSSTIGNYVVPQIVGALRGSTPQLRIDVDIGNSALIARKVAQFEIDMGLIEAPCHLPELIAE
PWLVDEMVIVAGRAHPLAGEEHVSLGELQSAEWLLREQGSGTREEVEHLLLRHLHDLQDMRQVGSSEAIMRTLAQGIGIS
CLSRRVVADLLASGQLRTLASPLPPLNRRFFMIRHRDKFISPSLERFREACRQAADQADTLRHGLSHNASPTSSSAP

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788481, Length=294, Percent_Identity=49.3197278911565, Blast_Score=276, Evalue=1e-75,
Organism=Escherichia coli, GI1788748, Length=304, Percent_Identity=26.3157894736842, Blast_Score=108, Evalue=3e-25,
Organism=Escherichia coli, GI157672245, Length=207, Percent_Identity=30.9178743961353, Blast_Score=98, Evalue=7e-22,
Organism=Escherichia coli, GI87082132, Length=291, Percent_Identity=24.7422680412371, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1788296, Length=246, Percent_Identity=27.2357723577236, Blast_Score=75, Evalue=7e-15,
Organism=Escherichia coli, GI87081904, Length=211, Percent_Identity=26.5402843601896, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1787601, Length=303, Percent_Identity=23.1023102310231, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1790399, Length=240, Percent_Identity=21.25, Blast_Score=68, Evalue=6e-13,
Organism=Escherichia coli, GI145693105, Length=289, Percent_Identity=22.1453287197232, Blast_Score=68, Evalue=9e-13,
Organism=Escherichia coli, GI1788887, Length=250, Percent_Identity=22.8, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI1789204, Length=258, Percent_Identity=24.031007751938, Blast_Score=65, Evalue=7e-12,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 34572; Mature: 34572

Theoretical pI: Translated: 7.27; Mature: 7.27

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRINLRQLQIFCAITRCGSTTSAGSALALSQSATSAALNELESALGTRLFDRVGKRLVLN
CCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEC
DSGQALLPRAMKMLENAQQIEDSFLLPDAALNARLRIGSSSTIGNYVVPQIVGALRGSTP
CCCCHHHHHHHHHHHHHHHHHHHHCCCCHHCCCEEEECCCCCCCHHHHHHHHHHHCCCCC
QLRIDVDIGNSALIARKVAQFEIDMGLIEAPCHLPELIAEPWLVDEMVIVAGRAHPLAGE
EEEEEEECCCCHHHHHHHHHHHHCCCHHHCCCCCHHHHHCCHHHHHHHHHCCCCCCCCCC
EHVSLGELQSAEWLLREQGSGTREEVEHLLLRHLHDLQDMRQVGSSEAIMRTLAQGIGIS
CCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCHH
CLSRRVVADLLASGQLRTLASPLPPLNRRFFMIRHRDKFISPSLERFREACRQAADQADT
HHHHHHHHHHHCCCCHHHHHCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHH
LRHGLSHNASPTSSSAP
HHHHCCCCCCCCCCCCC
>Mature Secondary Structure
MRINLRQLQIFCAITRCGSTTSAGSALALSQSATSAALNELESALGTRLFDRVGKRLVLN
CCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEC
DSGQALLPRAMKMLENAQQIEDSFLLPDAALNARLRIGSSSTIGNYVVPQIVGALRGSTP
CCCCHHHHHHHHHHHHHHHHHHHHCCCCHHCCCEEEECCCCCCCHHHHHHHHHHHCCCCC
QLRIDVDIGNSALIARKVAQFEIDMGLIEAPCHLPELIAEPWLVDEMVIVAGRAHPLAGE
EEEEEEECCCCHHHHHHHHHHHHCCCHHHCCCCCHHHHHCCHHHHHHHHHCCCCCCCCCC
EHVSLGELQSAEWLLREQGSGTREEVEHLLLRHLHDLQDMRQVGSSEAIMRTLAQGIGIS
CCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCHH
CLSRRVVADLLASGQLRTLASPLPPLNRRFFMIRHRDKFISPSLERFREACRQAADQADT
HHHHHHHHHHHCCCCHHHHHCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHH
LRHGLSHNASPTSSSAP
HHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]