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The map label for this gene is pcpR [H]

Identifier: 66046550

GI number: 66046550

Start: 3972243

End: 3973178

Strand: Reverse

Name: pcpR [H]

Synonym: Psyr_3321

Alternate gene names: 66046550

Gene position: 3973178-3972243 (Counterclockwise)

Preceding gene: 66046551

Following gene: 66046541

Centisome position: 65.2

GC content: 58.87

Gene sequence:

>936_bases
ATGCTTAACAGTAATGCCCTGAGAAAGCTGGACATGCAGGACCTCATGGTCTTTGTCTCGGTGTATGAGCAGCGCAACCT
GACCCTGGTCGCGGAAGCGTTGCATGTCAGCCAGTCGACGGTCAGTTACTGCCTGAAAAAGCTGCGTGCCAACTTCGAGG
ACGATCTGTTCATCTGCACCCGCAACGGCATGCGTCCGACCCGCAAGGCGTTGGCCATGCATGACCATGTGCAGCAGATT
CTCCGCTCGGTCAACCTGTGTCATGACGGTCTCGATCTGTTCGACCCGACTCGCGCACAGACCACCTTCACGGTCTGCGC
GCCTGAGTATTTCGAGCTGCTGGTATTGCCGCATTTGCTGCGTGACTTCGTTGCAGCCGGCTTCTCGGTGACGGTCAATG
TGCAAAAGCTGGAGAAGCAGTTGCCTGCCGAGCAATTGAACGACGGTCTGTTTGACCTCGCCCTGTGCTTCGGGCCTGGC
TTTCACCGAGTCCCGGACAGCCTGATTTCGCAGGTGTTGATGGAGGATGATCTGGTTTGCATCATGGACTCACGGTTCGC
GCCGGTCAGCGCCCTGATCGACCTGCAAACCTTCATCCGGCACCGTCATGTCTACCCGACGCCTTGGACGTCCGACACCA
ACATGGTGGATGGCTGGCTGCAGCGCCAGGGGCTTGAGCGCCACATCGTCGCGCGCGCCAACAGCTACCGGGCGGCATTG
CAATTGCTGGAAGGCACCGATTTCATTCTGGTTCTGCCACGCCGTATTCAGACGCTGCTGGGCAATGAGCCGTGGATTTG
CGTATTCGAACTGCCACCTGACTTGCCCGGCTTCAGTCTGGATATGCTGTGGAGCGCCCAGGCGGATCGAGACGAAGCCA
GTGGCTGGCTGCGTGAACAAGTCGTGAAGGTGTGTGCCGAGCGCGGCTTGCTCTGA

Upstream 100 bases:

>100_bases
AACCTGTCATCACTGGCAGACAGGCTGAATGATTGCATTTTGATGCTATCTTGTGCGCGTTGACCTGACCGCCCGGTCAT
AGCGCCAGCGAGATGCCTTG

Downstream 100 bases:

>100_bases
CGCGGCGTCAGTTGCCTGACTTGTCTTTCTGCGGGTCGGGAGAGGTGGCAGGTCGGGCCTGTTCACCCTGCTGATTGAGC
TGTTTGTTCTTCTCGCCCAC

Product: helix-turn-helix, Fis-type

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 311; Mature: 311

Protein sequence:

>311_residues
MLNSNALRKLDMQDLMVFVSVYEQRNLTLVAEALHVSQSTVSYCLKKLRANFEDDLFICTRNGMRPTRKALAMHDHVQQI
LRSVNLCHDGLDLFDPTRAQTTFTVCAPEYFELLVLPHLLRDFVAAGFSVTVNVQKLEKQLPAEQLNDGLFDLALCFGPG
FHRVPDSLISQVLMEDDLVCIMDSRFAPVSALIDLQTFIRHRHVYPTPWTSDTNMVDGWLQRQGLERHIVARANSYRAAL
QLLEGTDFILVLPRRIQTLLGNEPWICVFELPPDLPGFSLDMLWSAQADRDEASGWLREQVVKVCAERGLL

Sequences:

>Translated_311_residues
MLNSNALRKLDMQDLMVFVSVYEQRNLTLVAEALHVSQSTVSYCLKKLRANFEDDLFICTRNGMRPTRKALAMHDHVQQI
LRSVNLCHDGLDLFDPTRAQTTFTVCAPEYFELLVLPHLLRDFVAAGFSVTVNVQKLEKQLPAEQLNDGLFDLALCFGPG
FHRVPDSLISQVLMEDDLVCIMDSRFAPVSALIDLQTFIRHRHVYPTPWTSDTNMVDGWLQRQGLERHIVARANSYRAAL
QLLEGTDFILVLPRRIQTLLGNEPWICVFELPPDLPGFSLDMLWSAQADRDEASGWLREQVVKVCAERGLL
>Mature_311_residues
MLNSNALRKLDMQDLMVFVSVYEQRNLTLVAEALHVSQSTVSYCLKKLRANFEDDLFICTRNGMRPTRKALAMHDHVQQI
LRSVNLCHDGLDLFDPTRAQTTFTVCAPEYFELLVLPHLLRDFVAAGFSVTVNVQKLEKQLPAEQLNDGLFDLALCFGPG
FHRVPDSLISQVLMEDDLVCIMDSRFAPVSALIDLQTFIRHRHVYPTPWTSDTNMVDGWLQRQGLERHIVARANSYRAAL
QLLEGTDFILVLPRRIQTLLGNEPWICVFELPPDLPGFSLDMLWSAQADRDEASGWLREQVVKVCAERGLL

Specific function: Transcriptional activator for the pcpA, pcpB and pcpE genes for pentachlorophenol (PCP) degradation. Essential for PCP degradation [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87081684, Length=313, Percent_Identity=21.7252396166134, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI1786820, Length=109, Percent_Identity=30.2752293577982, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI87081766, Length=259, Percent_Identity=24.3243243243243, Blast_Score=65, Evalue=8e-12,

Paralogues:

None

Copy number: 825 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 35410; Mature: 35410

Theoretical pI: Translated: 5.58; Mature: 5.58

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLNSNALRKLDMQDLMVFVSVYEQRNLTLVAEALHVSQSTVSYCLKKLRANFEDDLFICT
CCCCHHHHHHCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEE
RNGMRPTRKALAMHDHVQQILRSVNLCHDGLDLFDPTRAQTTFTVCAPEYFELLVLPHLL
CCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECHHHHHHHHHHHHH
RDFVAAGFSVTVNVQKLEKQLPAEQLNDGLFDLALCFGPGFHRVPDSLISQVLMEDDLVC
HHHHHCCCEEEEEHHHHHHHCCHHHHCCHHHHHHHHHCCCHHCCCHHHHHHHHHCCCEEE
IMDSRFAPVSALIDLQTFIRHRHVYPTPWTSDTNMVDGWLQRQGLERHIVARANSYRAAL
EECCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHH
QLLEGTDFILVLPRRIQTLLGNEPWICVFELPPDLPGFSLDMLWSAQADRDEASGWLREQ
HHHCCCCEEEECHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHCCCCCCCHHHHHHHHHH
VVKVCAERGLL
HHHHHHHCCCC
>Mature Secondary Structure
MLNSNALRKLDMQDLMVFVSVYEQRNLTLVAEALHVSQSTVSYCLKKLRANFEDDLFICT
CCCCHHHHHHCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEE
RNGMRPTRKALAMHDHVQQILRSVNLCHDGLDLFDPTRAQTTFTVCAPEYFELLVLPHLL
CCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECHHHHHHHHHHHHH
RDFVAAGFSVTVNVQKLEKQLPAEQLNDGLFDLALCFGPGFHRVPDSLISQVLMEDDLVC
HHHHHCCCEEEEEHHHHHHHCCHHHHCCHHHHHHHHHCCCHHCCCHHHHHHHHHCCCEEE
IMDSRFAPVSALIDLQTFIRHRHVYPTPWTSDTNMVDGWLQRQGLERHIVARANSYRAAL
EECCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHH
QLLEGTDFILVLPRRIQTLLGNEPWICVFELPPDLPGFSLDMLWSAQADRDEASGWLREQ
HHHCCCCEEEECHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHCCCCCCCHHHHHHHHHH
VVKVCAERGLL
HHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12169590 [H]