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The map label for this gene is wcaJ [H]

Identifier: 66046460

GI number: 66046460

Start: 3870074

End: 3871483

Strand: Direct

Name: wcaJ [H]

Synonym: Psyr_3231

Alternate gene names: 66046460

Gene position: 3870074-3871483 (Clockwise)

Preceding gene: 66046459

Following gene: 66046461

Centisome position: 63.51

GC content: 62.55

Gene sequence:

>1410_bases
ATGCGTACTTCAGTCCGCGGCATTCTGCGTGCCCACCAGTCAGCGCTTTCGCTCGCCCACCGCTTGCTGGACCTGGCTGT
CATCGTGCTGATCGGTTACTGGCAAACCGGGCTGACGGTCACCGACAGCACAGAGGCCTGGTCGCACATTTTCCTCGCGG
TTCTGGTGTTTCACTGGGTCAGCGAGTATCACCAGTTGTATGGCTCATGGCGTGGCGAGCGCATTCTGCGCGAGCTGACC
AAGGTCTTCAGTTACTGGGCGATGACCTTCATCACTCTGCTGTCGCTGAACAGCCTGCTGCTCAACCAGACGCAATTGCC
CGATAACGGCCAGATGAGCTGGTTCGCGCTGGCACTCGCCGTGCTGTGCGGCTACCGGCTGCTGATCCGTCTGGCCCTGC
ACACCCTGCGTCGGCGTGGCTTCAACACGCGCCGGGTGGCCATCGTCGGCACCGGGCAGGTCGGCGAGCGGCTGGCCCGG
TCCATCGCCCTGGCGCCCTGGATGGGCCTGATCCTGCTCGGCTTCTACGACTCGCACCCGCAGCAGATGAATCTGCAGGC
ACACAAGCGCCGCCCGCGAGTACTGGGTGACCTGCAGCAGTTGATCGACGATGCCCGCGCAGGCCGCATCGACAAGGTGT
ATATCACCCTCGCCTTCAGCGCCGAACCGCAACTGCGCGAGTTGATTACCGGCCTGAGCGACACCACCGCCTCGGTGTAC
CTGATTCCGGATGTGTTCATGTTCGACCTGCTGCATGCGCGCAGCGAAAGCATCAACGGCCTGGCCAGCATCAGCATTTT
CGACTCGCCCATGGACGGCGCCTGGAGCCTGGCCAAACGCGCCGAAGACATCGTGCTGTCGAGCCTGATTCTGTTGCTGA
TCGCTGTGCCGCTGCTGATCATCGCGGCAGCCATCAAGCTCACCTCCGCCGGGCCGGTGCTGTTCCGCCAGCGCCGCTAC
GGGCTGGACGGTCGCTCGATCATGGTCTGGAAATTCCGCAGCATGAGCGTCCAGGCAAACACCGACGTGGTGCTGCAGGC
GACGCGCAACGATACACGGGTAACACCGCTGGGCCGCTTTTTACGCCGGACTTCGCTGGATGAGCTGCCGCAGTTCTTCA
ACGTGCTGCTCGGCGACATGTCCATCGTCGGCCCACGCCCCCATGCCATTGCCCACAACGAACAGTACCGCAGGCAGGTC
AGCGGCTACATGTTGCGCCACAAGGTCAAGCCCGGCATCACCGGCTGGGCGCAGATCAACGGCTGGCGCGGCGAGACCGA
CACGCTGGACAAGATGCGCATGCGTGTCGAATTCGACCTGGAATACATCGAGCACTGGTCGATCTGGCTGGACCTGAAAA
TCATTCTGCTGACGCTGCTCAAGGGGTTCGTCAACAAGAACGCCTTCTGA

Upstream 100 bases:

>100_bases
CCGCCTCAGTCGCAACGGGTCGCCCAGGCATCACCCGGCCTCGGCAGCGAGCTGCCGAACCGTGTCGTCCCCCTGAAAAT
CGTCTGAGCGAGCCCTTCCC

Downstream 100 bases:

>100_bases
TGCCTGCGCTGACCTGAGCACCCACCGAACACCCTCGGTACGCAACACGAAAGGAGCAATGCCATGAAAGGAAAAACGTC
ATGAATCGAAGCATTACTGC

Product: sugar transferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 469; Mature: 469

Protein sequence:

>469_residues
MRTSVRGILRAHQSALSLAHRLLDLAVIVLIGYWQTGLTVTDSTEAWSHIFLAVLVFHWVSEYHQLYGSWRGERILRELT
KVFSYWAMTFITLLSLNSLLLNQTQLPDNGQMSWFALALAVLCGYRLLIRLALHTLRRRGFNTRRVAIVGTGQVGERLAR
SIALAPWMGLILLGFYDSHPQQMNLQAHKRRPRVLGDLQQLIDDARAGRIDKVYITLAFSAEPQLRELITGLSDTTASVY
LIPDVFMFDLLHARSESINGLASISIFDSPMDGAWSLAKRAEDIVLSSLILLLIAVPLLIIAAAIKLTSAGPVLFRQRRY
GLDGRSIMVWKFRSMSVQANTDVVLQATRNDTRVTPLGRFLRRTSLDELPQFFNVLLGDMSIVGPRPHAIAHNEQYRRQV
SGYMLRHKVKPGITGWAQINGWRGETDTLDKMRMRVEFDLEYIEHWSIWLDLKIILLTLLKGFVNKNAF

Sequences:

>Translated_469_residues
MRTSVRGILRAHQSALSLAHRLLDLAVIVLIGYWQTGLTVTDSTEAWSHIFLAVLVFHWVSEYHQLYGSWRGERILRELT
KVFSYWAMTFITLLSLNSLLLNQTQLPDNGQMSWFALALAVLCGYRLLIRLALHTLRRRGFNTRRVAIVGTGQVGERLAR
SIALAPWMGLILLGFYDSHPQQMNLQAHKRRPRVLGDLQQLIDDARAGRIDKVYITLAFSAEPQLRELITGLSDTTASVY
LIPDVFMFDLLHARSESINGLASISIFDSPMDGAWSLAKRAEDIVLSSLILLLIAVPLLIIAAAIKLTSAGPVLFRQRRY
GLDGRSIMVWKFRSMSVQANTDVVLQATRNDTRVTPLGRFLRRTSLDELPQFFNVLLGDMSIVGPRPHAIAHNEQYRRQV
SGYMLRHKVKPGITGWAQINGWRGETDTLDKMRMRVEFDLEYIEHWSIWLDLKIILLTLLKGFVNKNAF
>Mature_469_residues
MRTSVRGILRAHQSALSLAHRLLDLAVIVLIGYWQTGLTVTDSTEAWSHIFLAVLVFHWVSEYHQLYGSWRGERILRELT
KVFSYWAMTFITLLSLNSLLLNQTQLPDNGQMSWFALALAVLCGYRLLIRLALHTLRRRGFNTRRVAIVGTGQVGERLAR
SIALAPWMGLILLGFYDSHPQQMNLQAHKRRPRVLGDLQQLIDDARAGRIDKVYITLAFSAEPQLRELITGLSDTTASVY
LIPDVFMFDLLHARSESINGLASISIFDSPMDGAWSLAKRAEDIVLSSLILLLIAVPLLIIAAAIKLTSAGPVLFRQRRY
GLDGRSIMVWKFRSMSVQANTDVVLQATRNDTRVTPLGRFLRRTSLDELPQFFNVLLGDMSIVGPRPHAIAHNEQYRRQV
SGYMLRHKVKPGITGWAQINGWRGETDTLDKMRMRVEFDLEYIEHWSIWLDLKIILLTLLKGFVNKNAF

Specific function: Slime polysaccharide colanic acid biosynthesis. [C]

COG id: COG2148

COG function: function code M; Sugar transferases involved in lipopolysaccharide synthesis

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial sugar transferase family [H]

Homologues:

Organism=Escherichia coli, GI1788360, Length=471, Percent_Identity=48.1953290870488, Blast_Score=435, Evalue=1e-123,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003362
- InterPro:   IPR017475
- InterPro:   IPR017473 [H]

Pfam domain/function: PF02397 Bac_transf [H]

EC number: NA

Molecular weight: Translated: 53329; Mature: 53329

Theoretical pI: Translated: 10.50; Mature: 10.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRTSVRGILRAHQSALSLAHRLLDLAVIVLIGYWQTGLTVTDSTEAWSHIFLAVLVFHWV
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCHHHHHHHHHHHHHHHHH
SEYHQLYGSWRGERILRELTKVFSYWAMTFITLLSLNSLLLNQTQLPDNGQMSWFALALA
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHH
VLCGYRLLIRLALHTLRRRGFNTRRVAIVGTGQVGERLARSIALAPWMGLILLGFYDSHP
HHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCC
QQMNLQAHKRRPRVLGDLQQLIDDARAGRIDKVYITLAFSAEPQLRELITGLSDTTASVY
CCCCCHHHHCCCHHHHHHHHHHHHHHCCCEEEEEEEEEECCCHHHHHHHCCCCCCCCEEE
LIPDVFMFDLLHARSESINGLASISIFDSPMDGAWSLAKRAEDIVLSSLILLLIAVPLLI
ECHHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IAAAIKLTSAGPVLFRQRRYGLDGRSIMVWKFRSMSVQANTDVVLQATRNDTRVTPLGRF
HHHHHHHCCCCHHHHHHHHCCCCCCEEEEEEEECCEEECCCCEEEEECCCCCCCCHHHHH
LRRTSLDELPQFFNVLLGDMSIVGPRPHAIAHNEQYRRQVSGYMLRHKVKPGITGWAQIN
HHHCCHHHHHHHHHHHHHCHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEC
GWRGETDTLDKMRMRVEFDLEYIEHWSIWLDLKIILLTLLKGFVNKNAF
CCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MRTSVRGILRAHQSALSLAHRLLDLAVIVLIGYWQTGLTVTDSTEAWSHIFLAVLVFHWV
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCHHHHHHHHHHHHHHHHH
SEYHQLYGSWRGERILRELTKVFSYWAMTFITLLSLNSLLLNQTQLPDNGQMSWFALALA
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHH
VLCGYRLLIRLALHTLRRRGFNTRRVAIVGTGQVGERLARSIALAPWMGLILLGFYDSHP
HHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCC
QQMNLQAHKRRPRVLGDLQQLIDDARAGRIDKVYITLAFSAEPQLRELITGLSDTTASVY
CCCCCHHHHCCCHHHHHHHHHHHHHHCCCEEEEEEEEEECCCHHHHHHHCCCCCCCCEEE
LIPDVFMFDLLHARSESINGLASISIFDSPMDGAWSLAKRAEDIVLSSLILLLIAVPLLI
ECHHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IAAAIKLTSAGPVLFRQRRYGLDGRSIMVWKFRSMSVQANTDVVLQATRNDTRVTPLGRF
HHHHHHHCCCCHHHHHHHHCCCCCCEEEEEEEECCEEECCCCEEEEECCCCCCCCHHHHH
LRRTSLDELPQFFNVLLGDMSIVGPRPHAIAHNEQYRRQVSGYMLRHKVKPGITGWAQIN
HHHCCHHHHHHHHHHHHHCHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEC
GWRGETDTLDKMRMRVEFDLEYIEHWSIWLDLKIILLTLLKGFVNKNAF
CCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8759852; 9097040; 9278503 [H]