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The map label for this gene is dhaAF [H]

Identifier: 66046416

GI number: 66046416

Start: 3816926

End: 3817807

Strand: Reverse

Name: dhaAF [H]

Synonym: Psyr_3187

Alternate gene names: 66046416

Gene position: 3817807-3816926 (Counterclockwise)

Preceding gene: 66046439

Following gene: 66046413

Centisome position: 62.65

GC content: 57.14

Gene sequence:

>882_bases
ATGAACAGTGATGATGCTTTCCGCGTGACGTTTCACACTCAGCAGGTAGGAGACGTAAATGTGTTCTATCGCGAAGTCGG
ACCCAGGGACGCCCCGGTGCTTCTGCTGCTCCATGGGTTTCCGAGTTCCAGCCACATGTTTCGTGACCTGATGCCATTGT
TGGCGAGTCAGTACCGCCTGATCGCTCCCGATCTGCCCGGTTTCGGCAATACCAAAGCACCACCGCGCGGGCAGTTCGAT
TACACATTCGAAAACCTTTACACGGTGATCGAGGGCTTTACCGAGGCACTGGGCCTGAAGAAATACGCGTTATACCTATT
CGACTATGGCGCACCGACAGGCTTGCGGCTCGCAGCGGCGAACCCGGAGAAGGTTACTGCAATCATCAGTCAGAACGGCA
ATGCCTATCTGGAAGGATTCAGCGATCAATGGGGCCCGTGGCAAGCCTATTGGCGCGAGCCTTCGGCAGCCAATCGCGAA
GCCTGTCGCGCCTCGTTGTCACCGCAAGTCATCCGGGACTGGCAATACGGTACGGGAGCCGATCCTGAAAAGCTTTCTCC
CGATGGCTACAACCTCGACATCCTGTATATGGCGCGGCCGGGCGCAGAGGAAATCCAGCTTGACCTGATTCTTGACTACC
GCAGTAACGTAGCGGCTTACCCCTCGTTTCAGGAATACCTGCGCAAATATCAGCCTCCCTTGCTGGCGGTCTGGGGCAAG
CACGATCCGGCGTTTATACCTCCGGGCGCGCATGCTTATCGCAAGGACGTCCCGGCTGCTGAGGTGCATCTGCTGGATGC
AGGCCACTTTGCATTGGAAACACACGCGCCGGAGGTCGCCGAATACATTCGCGACTTTCTTTCGCGCACGCTGAAAGGCT
GA

Upstream 100 bases:

>100_bases
CAAGGTTTTACAAGCGTCCGCGATAGGCGCTGCTTCTGCTCTCGGGGCGTCGTTGGTGAGCGCCGCTTCTGCAAGCAATA
AACCTCTCAGGGAAAATGCA

Downstream 100 bases:

>100_bases
GCGGGGTGGTGGTGTGCTTGAGCAGGCATCTCCCTGGTGCCCGGGCTTACACAGGTAGCTGACATTTTCCATGGATAAAA
AAATCCCCGGCAACAGGCCG

Product: Alpha/beta hydrolase fold

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 293; Mature: 293

Protein sequence:

>293_residues
MNSDDAFRVTFHTQQVGDVNVFYREVGPRDAPVLLLLHGFPSSSHMFRDLMPLLASQYRLIAPDLPGFGNTKAPPRGQFD
YTFENLYTVIEGFTEALGLKKYALYLFDYGAPTGLRLAAANPEKVTAIISQNGNAYLEGFSDQWGPWQAYWREPSAANRE
ACRASLSPQVIRDWQYGTGADPEKLSPDGYNLDILYMARPGAEEIQLDLILDYRSNVAAYPSFQEYLRKYQPPLLAVWGK
HDPAFIPPGAHAYRKDVPAAEVHLLDAGHFALETHAPEVAEYIRDFLSRTLKG

Sequences:

>Translated_293_residues
MNSDDAFRVTFHTQQVGDVNVFYREVGPRDAPVLLLLHGFPSSSHMFRDLMPLLASQYRLIAPDLPGFGNTKAPPRGQFD
YTFENLYTVIEGFTEALGLKKYALYLFDYGAPTGLRLAAANPEKVTAIISQNGNAYLEGFSDQWGPWQAYWREPSAANRE
ACRASLSPQVIRDWQYGTGADPEKLSPDGYNLDILYMARPGAEEIQLDLILDYRSNVAAYPSFQEYLRKYQPPLLAVWGK
HDPAFIPPGAHAYRKDVPAAEVHLLDAGHFALETHAPEVAEYIRDFLSRTLKG
>Mature_293_residues
MNSDDAFRVTFHTQQVGDVNVFYREVGPRDAPVLLLLHGFPSSSHMFRDLMPLLASQYRLIAPDLPGFGNTKAPPRGQFD
YTFENLYTVIEGFTEALGLKKYALYLFDYGAPTGLRLAAANPEKVTAIISQNGNAYLEGFSDQWGPWQAYWREPSAANRE
ACRASLSPQVIRDWQYGTGADPEKLSPDGYNLDILYMARPGAEEIQLDLILDYRSNVAAYPSFQEYLRKYQPPLLAVWGK
HDPAFIPPGAHAYRKDVPAAEVHLLDAGHFALETHAPEVAEYIRDFLSRTLKG

Specific function: Catalyzes hydrolytic cleavage of carbon-halogen bonds in halogenated aliphatic compounds, leading to the formation of the corresponding primary alcohols, halide ions and protons. Has a broad substrate specificity, which includes mono- and di- chlorinated

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the haloalkane dehalogenase family. Type 2 subfamily [H]

Homologues:

Organism=Homo sapiens, GI218777837, Length=110, Percent_Identity=36.3636363636364, Blast_Score=69, Evalue=5e-12,
Organism=Saccharomyces cerevisiae, GI6324392, Length=281, Percent_Identity=46.2633451957295, Blast_Score=242, Evalue=4e-65,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR000639 [H]

Pfam domain/function: PF00561 Abhydrolase_1 [H]

EC number: =3.8.1.5 [H]

Molecular weight: Translated: 32905; Mature: 32905

Theoretical pI: Translated: 5.35; Mature: 5.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSDDAFRVTFHTQQVGDVNVFYREVGPRDAPVLLLLHGFPSSSHMFRDLMPLLASQYRL
CCCCCEEEEEEECCCCCCCEEEEECCCCCCCCEEEEEECCCCCHHHHHHHHHHHHCCCEE
IAPDLPGFGNTKAPPRGQFDYTFENLYTVIEGFTEALGLKKYALYLFDYGAPTGLRLAAA
ECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCHHHEEEEEEECCCCCCEEEEEC
NPEKVTAIISQNGNAYLEGFSDQWGPWQAYWREPSAANREACRASLSPQVIRDWQYGTGA
CCCCEEEEEECCCCEEEECCCCCCCCHHHHHCCCCCCCHHHHHHCCCHHHHHCCCCCCCC
DPEKLSPDGYNLDILYMARPGAEEIQLDLILDYRSNVAAYPSFQEYLRKYQPPLLAVWGK
CCCCCCCCCCCEEEEEEECCCCCCEEEEEEEECCCCCCCCCCHHHHHHHHCCCEEEEECC
HDPAFIPPGAHAYRKDVPAAEVHLLDAGHFALETHAPEVAEYIRDFLSRTLKG
CCCCCCCCCCHHHHHCCCCCEEEEEECCCEEEECCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNSDDAFRVTFHTQQVGDVNVFYREVGPRDAPVLLLLHGFPSSSHMFRDLMPLLASQYRL
CCCCCEEEEEEECCCCCCCEEEEECCCCCCCCEEEEEECCCCCHHHHHHHHHHHHCCCEE
IAPDLPGFGNTKAPPRGQFDYTFENLYTVIEGFTEALGLKKYALYLFDYGAPTGLRLAAA
ECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCHHHEEEEEEECCCCCCEEEEEC
NPEKVTAIISQNGNAYLEGFSDQWGPWQAYWREPSAANREACRASLSPQVIRDWQYGTGA
CCCCEEEEEECCCCEEEECCCCCCCCHHHHHCCCCCCCHHHHHHCCCHHHHHCCCCCCCC
DPEKLSPDGYNLDILYMARPGAEEIQLDLILDYRSNVAAYPSFQEYLRKYQPPLLAVWGK
CCCCCCCCCCCEEEEEEECCCCCCEEEEEEEECCCCCCCCCCHHHHHHHHCCCEEEEECC
HDPAFIPPGAHAYRKDVPAAEVHLLDAGHFALETHAPEVAEYIRDFLSRTLKG
CCCCCCCCCCHHHHHCCCCCEEEEEECCCEEEECCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10094681; 10735862 [H]