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The map label for this gene is cspD [H]

Identifier: 66046414

GI number: 66046414

Start: 3813654

End: 3813932

Strand: Direct

Name: cspD [H]

Synonym: Psyr_3185

Alternate gene names: 66046414

Gene position: 3813654-3813932 (Clockwise)

Preceding gene: 66046411

Following gene: 66046415

Centisome position: 62.58

GC content: 49.46

Gene sequence:

>279_bases
ATGATTGAAGGAAAAGTCAAGTGGTTCAACAATGCAAAAGGGTTTGGGTTCATAAACGCTGAAGGCAAGAGTGACGAGGA
TCTGTTCGTTCATTTCTCGGCCATCGAAATGGACGGTTACAAAACTCTCAAGGCTGGCCAGAAAGTCAGATTTGAAGTCG
CCCACGGCCCAAAAGGGCTTCAGGCGATAAAAATCAACAGCTCGGAAGAAAAAAAGCAGGATCCGGCAAAAGATACCCAG
CCGCTGCTTCAGCAGGACCACGCTCACGCGGACGCCTGA

Upstream 100 bases:

>100_bases
GGTAAAAAGGGTCCGCGCAGTCAACCAAAATTTTTTTGGGTGGGGAGCGGATTGAAGTGGATGATACTCCTGACGGAGTC
CTTTGCAGAGGGAGATGGTG

Downstream 100 bases:

>100_bases
TGCTCGTGTTGAAAAAATCAGTTGATGTATAACGGCAGCCATCAGAGCCCGCCGAATTGGCAGAAGCATGCAATGAGCTT
TGCCGGACACGTAAAGAGAC

Product: cold-shock protein, DNA-binding

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 92; Mature: 92

Protein sequence:

>92_residues
MIEGKVKWFNNAKGFGFINAEGKSDEDLFVHFSAIEMDGYKTLKAGQKVRFEVAHGPKGLQAIKINSSEEKKQDPAKDTQ
PLLQQDHAHADA

Sequences:

>Translated_92_residues
MIEGKVKWFNNAKGFGFINAEGKSDEDLFVHFSAIEMDGYKTLKAGQKVRFEVAHGPKGLQAIKINSSEEKKQDPAKDTQ
PLLQQDHAHADA
>Mature_92_residues
MIEGKVKWFNNAKGFGFINAEGKSDEDLFVHFSAIEMDGYKTLKAGQKVRFEVAHGPKGLQAIKINSSEEKKQDPAKDTQ
PLLQQDHAHADA

Specific function: Inhibits DNA Replication At Both Initiation And Elongation Steps, Most Probably By Binding To The Opened, Single- Stranded Regions At Replication Forks. Plays A Regulatory Role In Chromosomal Replication In Nutrient-Depleted Cells. [C]

COG id: COG1278

COG function: function code K; Cold shock proteins

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CSD (cold-shock) domain [H]

Homologues:

Organism=Escherichia coli, GI1787107, Length=69, Percent_Identity=62.3188405797101, Blast_Score=86, Evalue=4e-19,
Organism=Escherichia coli, GI1786841, Length=64, Percent_Identity=56.25, Blast_Score=75, Evalue=9e-16,
Organism=Escherichia coli, GI1788126, Length=66, Percent_Identity=53.030303030303, Blast_Score=71, Evalue=1e-14,
Organism=Escherichia coli, GI1789979, Length=66, Percent_Identity=54.5454545454545, Blast_Score=69, Evalue=6e-14,
Organism=Escherichia coli, GI1787839, Length=64, Percent_Identity=53.125, Blast_Score=65, Evalue=1e-12,
Organism=Escherichia coli, GI1787834, Length=64, Percent_Identity=50, Blast_Score=65, Evalue=1e-12,
Organism=Escherichia coli, GI2367114, Length=64, Percent_Identity=45.3125, Blast_Score=60, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019844
- InterPro:   IPR012156
- InterPro:   IPR011129
- InterPro:   IPR002059
- InterPro:   IPR012751
- InterPro:   IPR012340
- InterPro:   IPR016027 [H]

Pfam domain/function: PF00313 CSD [H]

EC number: NA

Molecular weight: Translated: 10228; Mature: 10228

Theoretical pI: Translated: 6.97; Mature: 6.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIEGKVKWFNNAKGFGFINAEGKSDEDLFVHFSAIEMDGYKTLKAGQKVRFEVAHGPKGL
CCCCCEEEECCCCCEEEEECCCCCCCEEEEEEEEEEECCCEECCCCCEEEEEECCCCCCE
QAIKINSSEEKKQDPAKDTQPLLQQDHAHADA
EEEEECCCCCCCCCCCCCCHHHHHHCCCCCCC
>Mature Secondary Structure
MIEGKVKWFNNAKGFGFINAEGKSDEDLFVHFSAIEMDGYKTLKAGQKVRFEVAHGPKGL
CCCCCEEEECCCCCEEEEECCCCCCCEEEEEEEEEEECCCEECCCCCEEEEEECCCCCCE
QAIKINSSEEKKQDPAKDTQPLLQQDHAHADA
EEEEECCCCCCCCCCCCCCHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]