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The map label for this gene is metG [H]

Identifier: 66045985

GI number: 66045985

Start: 3337287

End: 3338786

Strand: Reverse

Name: metG [H]

Synonym: Psyr_2749

Alternate gene names: 66045985

Gene position: 3338786-3337287 (Counterclockwise)

Preceding gene: 66045986

Following gene: 66045984

Centisome position: 54.79

GC content: 64.13

Gene sequence:

>1500_bases
ATGAGTAAGTTCATCGTCACCATCACGCCCCCGACCCCCAACGGCGATTTGCACATAGGCCATATCGCCGGGCCGTTTCT
GGCCGCTGATGTCTTCACCCGCGTGCAGCGTCAGCGTGGTCACGAGTGTGTGCTGGTGTCCTATTCCGACGACTACCAGT
CCTACATGCTGCGCAAAGGGCTGGAGCTGGGCGTCGACCCGGTCGAGCTGGCCAGGCGCAATTCCGACCGCATCGAGGCC
TCGCTGGCGGCCATCAACATACAGCCCGATAACTGGATGCGCCCGCACGACAACCCGTGGTTCGCCGAAGCAGTGGGTGA
AGTGTTCGGTGCGTTGCGTGATGCCGGCGCGATCGAGTGTCGGGATTCCAGCGAGCCGTATTGCCCTGATTGCGACGTCT
GGGGCTACGAGGCCTTTGCCCGGGGCAACTGCAATTACTGCGGTGCCGAGTCCGATGCCAGTCAGTGCGAGAACTGCGCC
CAGGCCCCTGACGCCGAGTTGATGACAGACTTGCGCTGCAAGCTCTGCAGTCAGCCCTCGCAATGGAAAAACGCCCATCG
CGCGTTTCTGAAACTGGCCGGGTTCAAGCCGCAGTTGCGTGACCGCCTGCTGGGCCAGTCATGGCGCAAGCCGCTCGACA
GCTGGTTGCGCGACAGCCTGGAGCACCTGCATGACTGGGGCGTCACGCGTCCGGGCGACGGCGGGCTTGATCTGCAGGCC
GACGGCTCGTGCCGGGTGCACACCTGGTTCATGGGGCTGGCGGGCTACATGGCGGCATTTCGGGAGTATGCCGACCGCGT
CGGGCGTCCGGAGCTGTTTCGTCAGTACTGGCAGTCAGGGCAGGGCACGCTGGTGCATTTTCTCGGCTTTGACTGTGTGT
TCAGCCACGCCATCGTCTATCCGGCACAACTGACGGCGCTGCACGACATCAAGGTCCGCCAGCGCTTCATGCCCAATCAG
TTTCTCAAGCTGGACGGCCTGAACCTGTCGACCAGTCGCAACCATGCGATCTGGGTCGGCGACCTGGCCCGTGAAGCCTG
CGCGGACAGTGCGCGCCTGTATCTGGCGAGTATCGCGCCGGAGGAAAGCGAAGGTGATTTCCGCCTGGCGCAGTTCCTTG
CATGGCGGGCAGAGGTGTTCAGCGAGTTTTTCCCGGCCTTGCTGGACGCCGGAAGCAATCGCGACGACCACTGGTGGAGC
GGCCTCTGCGGGGCGGACGCCGGACTGCTGGAAGCGTTGCGCGTGCAGTGGAGCAAAGCCTCGGAGCCTGCGCAGTTTTC
GATGAAACGCATGGCTCAGGTGCTGCTGGACGCGATCGCCATCACCCGCGGGCGTCTGGCCGAAGGACGCCCGGTCAGCC
ATCTGGCGGCATTCATCGCGGTGGCCGGCAAGGCGCTGATTCCGGACATGTCAGCGCAGATCATCAGCGCGTTCGCACTG
CCCGAGGCCCGCGTCCACGCCACGCTGATGAACGGCCCGGCCGCTGAATACTCCATCTGA

Upstream 100 bases:

>100_bases
AATGAGTCCGCCGAGGACTTCCACTTCTATGTGGTCTGGTGGGACATGAACTACGTGAATGCGTTCGTGGCGCAGCACAG
CGAGACCACGGGGTGCCTGG

Downstream 100 bases:

>100_bases
ACGGATTCACGACAATGTTTATCGATTATGAGATAGCGGTCATTGGCGCCGGGATCACCGGCGCCAGTATCGCGGCGAAG
CTGTGCAGCGCCGGTGTGTC

Product: methionyl-tRNA synthetase

Products: NA

Alternate protein names: Methionine--tRNA ligase; MetRS [H]

Number of amino acids: Translated: 499; Mature: 498

Protein sequence:

>499_residues
MSKFIVTITPPTPNGDLHIGHIAGPFLAADVFTRVQRQRGHECVLVSYSDDYQSYMLRKGLELGVDPVELARRNSDRIEA
SLAAINIQPDNWMRPHDNPWFAEAVGEVFGALRDAGAIECRDSSEPYCPDCDVWGYEAFARGNCNYCGAESDASQCENCA
QAPDAELMTDLRCKLCSQPSQWKNAHRAFLKLAGFKPQLRDRLLGQSWRKPLDSWLRDSLEHLHDWGVTRPGDGGLDLQA
DGSCRVHTWFMGLAGYMAAFREYADRVGRPELFRQYWQSGQGTLVHFLGFDCVFSHAIVYPAQLTALHDIKVRQRFMPNQ
FLKLDGLNLSTSRNHAIWVGDLAREACADSARLYLASIAPEESEGDFRLAQFLAWRAEVFSEFFPALLDAGSNRDDHWWS
GLCGADAGLLEALRVQWSKASEPAQFSMKRMAQVLLDAIAITRGRLAEGRPVSHLAAFIAVAGKALIPDMSAQIISAFAL
PEARVHATLMNGPAAEYSI

Sequences:

>Translated_499_residues
MSKFIVTITPPTPNGDLHIGHIAGPFLAADVFTRVQRQRGHECVLVSYSDDYQSYMLRKGLELGVDPVELARRNSDRIEA
SLAAINIQPDNWMRPHDNPWFAEAVGEVFGALRDAGAIECRDSSEPYCPDCDVWGYEAFARGNCNYCGAESDASQCENCA
QAPDAELMTDLRCKLCSQPSQWKNAHRAFLKLAGFKPQLRDRLLGQSWRKPLDSWLRDSLEHLHDWGVTRPGDGGLDLQA
DGSCRVHTWFMGLAGYMAAFREYADRVGRPELFRQYWQSGQGTLVHFLGFDCVFSHAIVYPAQLTALHDIKVRQRFMPNQ
FLKLDGLNLSTSRNHAIWVGDLAREACADSARLYLASIAPEESEGDFRLAQFLAWRAEVFSEFFPALLDAGSNRDDHWWS
GLCGADAGLLEALRVQWSKASEPAQFSMKRMAQVLLDAIAITRGRLAEGRPVSHLAAFIAVAGKALIPDMSAQIISAFAL
PEARVHATLMNGPAAEYSI
>Mature_498_residues
SKFIVTITPPTPNGDLHIGHIAGPFLAADVFTRVQRQRGHECVLVSYSDDYQSYMLRKGLELGVDPVELARRNSDRIEAS
LAAINIQPDNWMRPHDNPWFAEAVGEVFGALRDAGAIECRDSSEPYCPDCDVWGYEAFARGNCNYCGAESDASQCENCAQ
APDAELMTDLRCKLCSQPSQWKNAHRAFLKLAGFKPQLRDRLLGQSWRKPLDSWLRDSLEHLHDWGVTRPGDGGLDLQAD
GSCRVHTWFMGLAGYMAAFREYADRVGRPELFRQYWQSGQGTLVHFLGFDCVFSHAIVYPAQLTALHDIKVRQRFMPNQF
LKLDGLNLSTSRNHAIWVGDLAREACADSARLYLASIAPEESEGDFRLAQFLAWRAEVFSEFFPALLDAGSNRDDHWWSG
LCGADAGLLEALRVQWSKASEPAQFSMKRMAQVLLDAIAITRGRLAEGRPVSHLAAFIAVAGKALIPDMSAQIISAFALP
EARVHATLMNGPAAEYSI

Specific function: Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation [H]

COG id: COG0143

COG function: function code J; Methionyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 tRNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI14043022, Length=388, Percent_Identity=25.7731958762887, Blast_Score=105, Evalue=1e-22,
Organism=Escherichia coli, GI1788432, Length=382, Percent_Identity=24.6073298429319, Blast_Score=127, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI17540794, Length=385, Percent_Identity=24.1558441558442, Blast_Score=102, Evalue=6e-22,
Organism=Saccharomyces cerevisiae, GI6321704, Length=388, Percent_Identity=24.4845360824742, Blast_Score=108, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24655311, Length=382, Percent_Identity=25.130890052356, Blast_Score=115, Evalue=7e-26,

Paralogues:

None

Copy number: 871 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR015413
- InterPro:   IPR004495
- InterPro:   IPR014758
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR014729
- InterPro:   IPR002547
- InterPro:   IPR009080
- InterPro:   IPR013155 [H]

Pfam domain/function: PF08264 Anticodon_1; PF09334 tRNA-synt_1g; PF01588 tRNA_bind [H]

EC number: =6.1.1.10 [H]

Molecular weight: Translated: 55525; Mature: 55394

Theoretical pI: Translated: 5.99; Mature: 5.99

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKFIVTITPPTPNGDLHIGHIAGPFLAADVFTRVQRQRGHECVLVSYSDDYQSYMLRKG
CCCEEEEECCCCCCCCEEEECCCCHHHHHHHHHHHHHHCCCEEEEEEECCHHHHHHHHCC
LELGVDPVELARRNSDRIEASLAAINIQPDNWMRPHDNPWFAEAVGEVFGALRDAGAIEC
HHCCCCHHHHHHCCCHHHEEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEE
RDSSEPYCPDCDVWGYEAFARGNCNYCGAESDASQCENCAQAPDAELMTDLRCKLCSQPS
CCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHCCCCH
QWKNAHRAFLKLAGFKPQLRDRLLGQSWRKPLDSWLRDSLEHLHDWGVTRPGDGGLDLQA
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEC
DGSCRVHTWFMGLAGYMAAFREYADRVGRPELFRQYWQSGQGTLVHFLGFDCVFSHAIVY
CCCCEEHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHH
PAQLTALHDIKVRQRFMPNQFLKLDGLNLSTSRNHAIWVGDLAREACADSARLYLASIAP
HHHHHHHHHHHHHHHHCCCHHEEECCCCCCCCCCCEEEEHHHHHHHHHCCHHEEEEECCC
EESEGDFRLAQFLAWRAEVFSEFFPALLDAGSNRDDHWWSGLCGADAGLLEALRVQWSKA
CCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCCCCCHHHHHHHHHHHHCC
SEPAQFSMKRMAQVLLDAIAITRGRLAEGRPVSHLAAFIAVAGKALIPDMSAQIISAFAL
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHCC
PEARVHATLMNGPAAEYSI
CHHHHEEEEECCCCCCCCC
>Mature Secondary Structure 
SKFIVTITPPTPNGDLHIGHIAGPFLAADVFTRVQRQRGHECVLVSYSDDYQSYMLRKG
CCEEEEECCCCCCCCEEEECCCCHHHHHHHHHHHHHHCCCEEEEEEECCHHHHHHHHCC
LELGVDPVELARRNSDRIEASLAAINIQPDNWMRPHDNPWFAEAVGEVFGALRDAGAIEC
HHCCCCHHHHHHCCCHHHEEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEE
RDSSEPYCPDCDVWGYEAFARGNCNYCGAESDASQCENCAQAPDAELMTDLRCKLCSQPS
CCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHCCCCH
QWKNAHRAFLKLAGFKPQLRDRLLGQSWRKPLDSWLRDSLEHLHDWGVTRPGDGGLDLQA
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEC
DGSCRVHTWFMGLAGYMAAFREYADRVGRPELFRQYWQSGQGTLVHFLGFDCVFSHAIVY
CCCCEEHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHH
PAQLTALHDIKVRQRFMPNQFLKLDGLNLSTSRNHAIWVGDLAREACADSARLYLASIAP
HHHHHHHHHHHHHHHHCCCHHEEECCCCCCCCCCCEEEEHHHHHHHHHCCHHEEEEECCC
EESEGDFRLAQFLAWRAEVFSEFFPALLDAGSNRDDHWWSGLCGADAGLLEALRVQWSKA
CCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCCCCCHHHHHHHHHHHHCC
SEPAQFSMKRMAQVLLDAIAITRGRLAEGRPVSHLAAFIAVAGKALIPDMSAQIISAFAL
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHCC
PEARVHATLMNGPAAEYSI
CHHHHEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA