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The map label for this gene is potF [H]

Identifier: 66045749

GI number: 66045749

Start: 2922800

End: 2923879

Strand: Direct

Name: potF [H]

Synonym: Psyr_2513

Alternate gene names: 66045749

Gene position: 2922800-2923879 (Clockwise)

Preceding gene: 66045747

Following gene: 66045754

Centisome position: 47.96

GC content: 57.13

Gene sequence:

>1080_bases
ATGCTGAAAAAATCCCTTGCCGCACTGGTGTTGCTGGCCTCGACCAGTCACGCGGCAGAGACGGTCAATATCTACAACTG
GACGGACTATATAGCGCCGGACACCCTGAAAAATTTCGAAGCAGCCACCGGCTTCAAGACCACTTACGAAACCTTTGAAA
CCAATGAGGCGCTGAACGCAAAACTGCTGGCTGGCCACTCGGGCCACGATGTGGTTTTTCCGTCCATTCATTTCATGGGA
CGACAAATCCAGAGCGGACTGCTCAAAACCCTCGACAAACGGCAGTTGCCCAACTGGAAAAACCTCAACCCGGTCCTGCT
CAAGGCGCTGGAGGGCAGTGACCCCGGTAATCAGCATGGCTTCCCTTATCTGTGGGGCAGTACCGGCATCGGCTATGACA
CAACCAAGGTCAAGGCCATCCTCGGCAAGGATGCACCGCTCGATTCCTGGGATCTGGTGCTCAAGCCGGAAAACATGAAA
AAGCTCGCCCAATGCGGTGTGGTATTTCTCGACAGCGCGCCGGCTCTGCTGCCGATCACCCTGAATTACCTTGGCTTGCC
ACCACACAGCGAAGTGCCTGACGACTACCGCCAGGCAAAGGCGGTACTGGACAGCGTGCGGCCATACGTACGCAATTTCA
GTTCTGCCGATTACATCGCCGACCTCGCGAAGGGCAGAATTTGTGTCGCGGTGGGCTATTCCGGGGATATTTCCCAGGCT
CAGGAGCAGGCACAAAAAGAAGGCAATGGCATCACCATCGACTATGCCGTTCCGAAGGAGGGCGCGCCCATGTGGTTCGA
CATGGTCGCCATCCCTGCGGACGCCACCGATATCAAGGGCGCCTACGCGTTCCTGAATTACCTGCTGCGCCCCGAAGTGA
TCGCCAACATCACCAATACCGTGCATTACGCCAACGGCAATGAAAAGGCAGACGCGCTGATCGGTCCCGGCCTGTGGACC
GATACCACGGTGTACCCGGACGCCGACATGTTGAGCCGCCTGTTCGTGATGTCTCAGGTCCCGGACACTATCGATGCGTT
GCGCGAGAAGCTCTGGGACAGTTTCAGGGCCGACAGGTAA

Upstream 100 bases:

>100_bases
CGCTGCCGCAAGTCCTTCAATGGAATGAATTTGCCGTGACGAAGATGGTTTTCGTGCCGGGTCTGGGGCAAGCTGTCGCT
ATTGACCCGAGGAACCGCAC

Downstream 100 bases:

>100_bases
CGTTGCTCAGATGACATGAGCCTGTTGCAGTTCGGTCAGTGCCTGAGCCTTCAACCGGGCCAGCAGCGGATCGCGACGAT
CCCTTGGCGTCTGCAGCCCG

Product: extracellular solute-binding protein

Products: ADP; phosphate; putrescine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 359; Mature: 359

Protein sequence:

>359_residues
MLKKSLAALVLLASTSHAAETVNIYNWTDYIAPDTLKNFEAATGFKTTYETFETNEALNAKLLAGHSGHDVVFPSIHFMG
RQIQSGLLKTLDKRQLPNWKNLNPVLLKALEGSDPGNQHGFPYLWGSTGIGYDTTKVKAILGKDAPLDSWDLVLKPENMK
KLAQCGVVFLDSAPALLPITLNYLGLPPHSEVPDDYRQAKAVLDSVRPYVRNFSSADYIADLAKGRICVAVGYSGDISQA
QEQAQKEGNGITIDYAVPKEGAPMWFDMVAIPADATDIKGAYAFLNYLLRPEVIANITNTVHYANGNEKADALIGPGLWT
DTTVYPDADMLSRLFVMSQVPDTIDALREKLWDSFRADR

Sequences:

>Translated_359_residues
MLKKSLAALVLLASTSHAAETVNIYNWTDYIAPDTLKNFEAATGFKTTYETFETNEALNAKLLAGHSGHDVVFPSIHFMG
RQIQSGLLKTLDKRQLPNWKNLNPVLLKALEGSDPGNQHGFPYLWGSTGIGYDTTKVKAILGKDAPLDSWDLVLKPENMK
KLAQCGVVFLDSAPALLPITLNYLGLPPHSEVPDDYRQAKAVLDSVRPYVRNFSSADYIADLAKGRICVAVGYSGDISQA
QEQAQKEGNGITIDYAVPKEGAPMWFDMVAIPADATDIKGAYAFLNYLLRPEVIANITNTVHYANGNEKADALIGPGLWT
DTTVYPDADMLSRLFVMSQVPDTIDALREKLWDSFRADR
>Mature_359_residues
MLKKSLAALVLLASTSHAAETVNIYNWTDYIAPDTLKNFEAATGFKTTYETFETNEALNAKLLAGHSGHDVVFPSIHFMG
RQIQSGLLKTLDKRQLPNWKNLNPVLLKALEGSDPGNQHGFPYLWGSTGIGYDTTKVKAILGKDAPLDSWDLVLKPENMK
KLAQCGVVFLDSAPALLPITLNYLGLPPHSEVPDDYRQAKAVLDSVRPYVRNFSSADYIADLAKGRICVAVGYSGDISQA
QEQAQKEGNGITIDYAVPKEGAPMWFDMVAIPADATDIKGAYAFLNYLLRPEVIANITNTVHYANGNEKADALIGPGLWT
DTTVYPDADMLSRLFVMSQVPDTIDALREKLWDSFRADR

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine. Polyamine binding protein [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein potD/potF family [H]

Homologues:

Organism=Escherichia coli, GI1787078, Length=355, Percent_Identity=47.3239436619718, Blast_Score=352, Evalue=2e-98,
Organism=Escherichia coli, GI1787367, Length=321, Percent_Identity=31.7757009345794, Blast_Score=166, Evalue=2e-42,
Organism=Escherichia coli, GI1787711, Length=323, Percent_Identity=22.6006191950464, Blast_Score=75, Evalue=6e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR001188 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 39455; Mature: 39455

Theoretical pI: Translated: 5.21; Mature: 5.21

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKKSLAALVLLASTSHAAETVNIYNWTDYIAPDTLKNFEAATGFKTTYETFETNEALNA
CCHHHHHHHHHHHCCCCCHHEEEEECCCCCCCCHHHHHCHHHCCCCHHHHHHHCCCCCCE
KLLAGHSGHDVVFPSIHFMGRQIQSGLLKTLDKRQLPNWKNLNPVLLKALEGSDPGNQHG
EEEECCCCCCEECCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCCCCCCCC
FPYLWGSTGIGYDTTKVKAILGKDAPLDSWDLVLKPENMKKLAQCGVVFLDSAPALLPIT
CCEEECCCCCCCCHHHHHHHHCCCCCCCCCEEEECCHHHHHHHHCCEEEECCCCCEEEEE
LNYLGLPPHSEVPDDYRQAKAVLDSVRPYVRNFSSADYIADLAKGRICVAVGYSGDISQA
EEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCEEEEECCCCCHHHH
QEQAQKEGNGITIDYAVPKEGAPMWFDMVAIPADATDIKGAYAFLNYLLRPEVIANITNT
HHHHHHCCCCEEEEEECCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHCHHHHHHHHHH
VHYANGNEKADALIGPGLWTDTTVYPDADMLSRLFVMSQVPDTIDALREKLWDSFRADR
EEECCCCCCCCEEECCCCCCCCEECCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MLKKSLAALVLLASTSHAAETVNIYNWTDYIAPDTLKNFEAATGFKTTYETFETNEALNA
CCHHHHHHHHHHHCCCCCHHEEEEECCCCCCCCHHHHHCHHHCCCCHHHHHHHCCCCCCE
KLLAGHSGHDVVFPSIHFMGRQIQSGLLKTLDKRQLPNWKNLNPVLLKALEGSDPGNQHG
EEEECCCCCCEECCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCCCCCCCC
FPYLWGSTGIGYDTTKVKAILGKDAPLDSWDLVLKPENMKKLAQCGVVFLDSAPALLPIT
CCEEECCCCCCCCHHHHHHHHCCCCCCCCCEEEECCHHHHHHHHCCEEEECCCCCEEEEE
LNYLGLPPHSEVPDDYRQAKAVLDSVRPYVRNFSSADYIADLAKGRICVAVGYSGDISQA
EEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCEEEEECCCCCHHHH
QEQAQKEGNGITIDYAVPKEGAPMWFDMVAIPADATDIKGAYAFLNYLLRPEVIANITNT
HHHHHHCCCCEEEEEECCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHCHHHHHHHHHH
VHYANGNEKADALIGPGLWTDTTVYPDADMLSRLFVMSQVPDTIDALREKLWDSFRADR
EEECCCCCCCCEEECCCCCCCCEECCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; putrescine [Periplasm]; H2O [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] [C]

General reaction: Polyamine Binding Protein [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8416922; 8905232; 9278503; 9298646; 9651355 [H]