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The map label for this gene is nfuA [H]

Identifier: 66045702

GI number: 66045702

Start: 2858125

End: 2858718

Strand: Direct

Name: nfuA [H]

Synonym: Psyr_2466

Alternate gene names: 66045702

Gene position: 2858125-2858718 (Clockwise)

Preceding gene: 66045701

Following gene: 66045710

Centisome position: 46.9

GC content: 57.58

Gene sequence:

>594_bases
ATGACCGCTATCACTATTACCGATGCCGCCCACGATTATCTGGCCGATCTGCTGGAAAAGCAGAATACCCCGGGCATTGG
CATCCGCGTATTTATCACCCAGCCCGGCACTCAGTACGCCGAAACCTGCATTGCCTACTGCAAGCCGGGTGAAGAAAAAC
CGGAAGACAAGGCCATCGGCCTGAAGAGCTTTACGGCCTGGATCGACGGCTTCAGCGAGGCCTTTCTCGATGATGCAGTG
GTCGACTACGCCACCGACCGGATGGGCGGGCAGCTGACTATCAAGGCACCGAACGCCAAAGTGCCGATGGTCAATGCCGA
CAGCCCGATCAACGAGCGCATCAATTACTACCTGCAAACCGAGATCAATCCGGGCCTCGCCAGCCATGGCGGTCAGGTCA
CGCTGATCGATGTGGTCGAGGAAGAGACGAAGAACATCGCCGTGCTGCAGTTCGGCGGTGGTTGCCAGGGTTGTGGCCAG
GCCGATGTCACGCTGAAGGAAGGTATCGAGCGCACGTTGCTTGAGCGTATTCCCGAGTTGTCGGGCGTTCGCGATGTTAC
CGACCATACGCAGAAAGAAAACGCCTACTACTAG

Upstream 100 bases:

>100_bases
GCGGTCAGTCAGGCGCAGGCATTAACCTGACAAAAATACTGGGACTTTGTGTGACGCGGCCGATTGGCGTAAACTGCACG
CACGTCTGCGAGGAATTTTC

Downstream 100 bases:

>100_bases
GCGTCTCTTTTTTGCAAGGAAGTTGCTGCACGAAAGTTGCTGCACGAAAACGCCCTGACTGTTCAGGGCGTTTTCATGTG
TGGCGGTTGACGAGCGTCAG

Product: HesB/YadR/YfhF:nitrogen-fixing NifU, C-terminal

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 197; Mature: 196

Protein sequence:

>197_residues
MTAITITDAAHDYLADLLEKQNTPGIGIRVFITQPGTQYAETCIAYCKPGEEKPEDKAIGLKSFTAWIDGFSEAFLDDAV
VDYATDRMGGQLTIKAPNAKVPMVNADSPINERINYYLQTEINPGLASHGGQVTLIDVVEEETKNIAVLQFGGGCQGCGQ
ADVTLKEGIERTLLERIPELSGVRDVTDHTQKENAYY

Sequences:

>Translated_197_residues
MTAITITDAAHDYLADLLEKQNTPGIGIRVFITQPGTQYAETCIAYCKPGEEKPEDKAIGLKSFTAWIDGFSEAFLDDAV
VDYATDRMGGQLTIKAPNAKVPMVNADSPINERINYYLQTEINPGLASHGGQVTLIDVVEEETKNIAVLQFGGGCQGCGQ
ADVTLKEGIERTLLERIPELSGVRDVTDHTQKENAYY
>Mature_196_residues
TAITITDAAHDYLADLLEKQNTPGIGIRVFITQPGTQYAETCIAYCKPGEEKPEDKAIGLKSFTAWIDGFSEAFLDDAVV
DYATDRMGGQLTIKAPNAKVPMVNADSPINERINYYLQTEINPGLASHGGQVTLIDVVEEETKNIAVLQFGGGCQGCGQA
DVTLKEGIERTLLERIPELSGVRDVTDHTQKENAYY

Specific function: Involved in iron-sulfur cluster biogenesis. Binds a 4Fe- 4S cluster, can transfer this cluster to apoproteins, and thereby intervenes in the maturation of Fe/S proteins. Could also act as a scaffold/chaperone for damaged Fe/S proteins [H]

COG id: COG0316

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nfuA family [H]

Homologues:

Organism=Escherichia coli, GI1789819, Length=194, Percent_Identity=49.4845360824742, Blast_Score=199, Evalue=1e-52,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017726
- InterPro:   IPR000361
- InterPro:   IPR001075
- ProDom:   PD002830 [H]

Pfam domain/function: PF01521 Fe-S_biosyn; PF01106 NifU [H]

EC number: NA

Molecular weight: Translated: 21472; Mature: 21341

Theoretical pI: Translated: 4.26; Mature: 4.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAITITDAAHDYLADLLEKQNTPGIGIRVFITQPGTQYAETCIAYCKPGEEKPEDKAIG
CEEEEEEHHHHHHHHHHHHHCCCCCCEEEEEEECCCHHHHHHHHHHCCCCCCCCCCHHCC
LKSFTAWIDGFSEAFLDDAVVDYATDRMGGQLTIKAPNAKVPMVNADSPINERINYYLQT
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCEEECCCCHHCCCEEEEEE
EINPGLASHGGQVTLIDVVEEETKNIAVLQFGGGCQGCGQADVTLKEGIERTLLERIPEL
ECCCCCCCCCCEEEEEEEEHHCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHCHHH
SGVRDVTDHTQKENAYY
CCCCHHHHHHCCCCCCC
>Mature Secondary Structure 
TAITITDAAHDYLADLLEKQNTPGIGIRVFITQPGTQYAETCIAYCKPGEEKPEDKAIG
EEEEEEHHHHHHHHHHHHHCCCCCCEEEEEEECCCHHHHHHHHHHCCCCCCCCCCHHCC
LKSFTAWIDGFSEAFLDDAVVDYATDRMGGQLTIKAPNAKVPMVNADSPINERINYYLQT
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCEEECCCCHHCCCEEEEEE
EINPGLASHGGQVTLIDVVEEETKNIAVLQFGGGCQGCGQADVTLKEGIERTLLERIPEL
ECCCCCCCCCCEEEEEEEEHHCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHCHHH
SGVRDVTDHTQKENAYY
CCCCHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA