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The map label for this gene is dmlR [H]

Identifier: 66045633

GI number: 66045633

Start: 2779273

End: 2780187

Strand: Direct

Name: dmlR [H]

Synonym: Psyr_2397

Alternate gene names: 66045633

Gene position: 2779273-2780187 (Clockwise)

Preceding gene: 66045631

Following gene: 66045639

Centisome position: 45.61

GC content: 62.51

Gene sequence:

>915_bases
GTGAATAAATATCCCAGCCTCGATGATTTGAATGTGTTCGTTCATGTGGCCCGGCGTTCAAGCTTCGTCGGGGCTGCGGA
TGAGCTGGGCATGTCCGCGGCCTACGTCAGCAAACGTATTCGTTTGCTGGAGCAGGGTATGGGTGTGGTGTTGCTGCACC
GCTCGACCCGGCAGGTGTCGATTACCGAAGATGGCGAGCGCGTCTACGAGTGGGCCAAGGGCATGCTCGATTCAGTGCAG
CAGATGGGTGACGAAGTGGCGGCGCTGCACGGCGAGCCGAGTGGCTTGTTGCGGGTCGTCAGCAGTCAGGGCTTTGGCCG
CCGTTTCGTCGCGCCTGCGTTGTCGGAGCTGGCGGCGCGTTATCCGAAGCTGGATATCCGCCTGGACATTCAGGACCGGC
TGGTCGATCTGATCGAGGAGGGCGTCGATCTGGATATCCGCGTCGGCAACGAAATCGCCCCGCACCTGCGCGCCAGGCAT
CTGGCCGACAACTGGCGGGTTTTGTGCGCGAGCCCCGGCTACCTCGACGTTCATGGCACGCCGCTTAATCTTGGTGAGCT
GGCCAATCACGATTGCCTGGTGATCAAGGAGCGCGACCGGCCATTCGGCATCTGGCACCTCAACGGGCCGCAGGGTGCCG
AAACCGTCAAGGTGACCGGCAGCCTGTCTACCAACCACGGCGAAATCGCCCGTCAGTGGTGCCTGGACGGGCGCGGGGTG
TTGCTGCGTTCGCTGTGGGATGTGCGCACTGACCTGGCCGAGGGCCGGCTGGTTCAGGTGTTGCCGGATTACCGTCAGGA
TGCAGACATCTGGGCGGTGCACACCTCGCCGCTGATGAGTTCGGCCAAGGTGCGCGTCACGGTAGAGTTTCTGCGCGACT
ACCTTGCCCTGCATCCTTCACCCGTCAACCACTAA

Upstream 100 bases:

>100_bases
CATTACGGGTGTGCGATGAATGAGTTGCACAGGCCATCCATGCTATAAGCGCACGATATAAAGATAATCAGCTGATTAAT
GGACTGAGAAAACACGAATC

Downstream 100 bases:

>100_bases
GGCACTATCAGTGCGTGCCGGCGGCCTTGTTCAGGTCGCTGTCGGTCCACTCGGTGTAGATGCACGCGTCTGCCGCGGCC
CAACGCACGCGCACCGGGTC

Product: regulatory protein, LysR:LysR, substrate-binding

Products: NA

Alternate protein names: D-malate degradation protein R [H]

Number of amino acids: Translated: 304; Mature: 304

Protein sequence:

>304_residues
MNKYPSLDDLNVFVHVARRSSFVGAADELGMSAAYVSKRIRLLEQGMGVVLLHRSTRQVSITEDGERVYEWAKGMLDSVQ
QMGDEVAALHGEPSGLLRVVSSQGFGRRFVAPALSELAARYPKLDIRLDIQDRLVDLIEEGVDLDIRVGNEIAPHLRARH
LADNWRVLCASPGYLDVHGTPLNLGELANHDCLVIKERDRPFGIWHLNGPQGAETVKVTGSLSTNHGEIARQWCLDGRGV
LLRSLWDVRTDLAEGRLVQVLPDYRQDADIWAVHTSPLMSSAKVRVTVEFLRDYLALHPSPVNH

Sequences:

>Translated_304_residues
MNKYPSLDDLNVFVHVARRSSFVGAADELGMSAAYVSKRIRLLEQGMGVVLLHRSTRQVSITEDGERVYEWAKGMLDSVQ
QMGDEVAALHGEPSGLLRVVSSQGFGRRFVAPALSELAARYPKLDIRLDIQDRLVDLIEEGVDLDIRVGNEIAPHLRARH
LADNWRVLCASPGYLDVHGTPLNLGELANHDCLVIKERDRPFGIWHLNGPQGAETVKVTGSLSTNHGEIARQWCLDGRGV
LLRSLWDVRTDLAEGRLVQVLPDYRQDADIWAVHTSPLMSSAKVRVTVEFLRDYLALHPSPVNH
>Mature_304_residues
MNKYPSLDDLNVFVHVARRSSFVGAADELGMSAAYVSKRIRLLEQGMGVVLLHRSTRQVSITEDGERVYEWAKGMLDSVQ
QMGDEVAALHGEPSGLLRVVSSQGFGRRFVAPALSELAARYPKLDIRLDIQDRLVDLIEEGVDLDIRVGNEIAPHLRARH
LADNWRVLCASPGYLDVHGTPLNLGELANHDCLVIKERDRPFGIWHLNGPQGAETVKVTGSLSTNHGEIARQWCLDGRGV
LLRSLWDVRTDLAEGRLVQVLPDYRQDADIWAVHTSPLMSSAKVRVTVEFLRDYLALHPSPVNH

Specific function: Transcriptional regulator required for the aerobic growth on D-malate as the sole carbon source. Induces the expression of dmlA in response to D-malate or L- or meso-tartrate. Negatively regulates its own expression [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87081978, Length=298, Percent_Identity=59.7315436241611, Blast_Score=364, Evalue=1e-102,
Organism=Escherichia coli, GI1789440, Length=291, Percent_Identity=47.0790378006873, Blast_Score=284, Evalue=5e-78,
Organism=Escherichia coli, GI1786401, Length=294, Percent_Identity=31.6326530612245, Blast_Score=162, Evalue=2e-41,
Organism=Escherichia coli, GI1789639, Length=291, Percent_Identity=30.9278350515464, Blast_Score=128, Evalue=4e-31,
Organism=Escherichia coli, GI1787128, Length=262, Percent_Identity=29.7709923664122, Blast_Score=119, Evalue=4e-28,
Organism=Escherichia coli, GI145693193, Length=260, Percent_Identity=31.5384615384615, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI1787589, Length=267, Percent_Identity=26.5917602996255, Blast_Score=98, Evalue=6e-22,
Organism=Escherichia coli, GI1788706, Length=262, Percent_Identity=27.8625954198473, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1789173, Length=264, Percent_Identity=29.1666666666667, Blast_Score=88, Evalue=7e-19,
Organism=Escherichia coli, GI1786448, Length=253, Percent_Identity=26.8774703557312, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI157672245, Length=155, Percent_Identity=30.3225806451613, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1787601, Length=134, Percent_Identity=26.865671641791, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1786713, Length=212, Percent_Identity=29.2452830188679, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI1788748, Length=137, Percent_Identity=32.1167883211679, Blast_Score=65, Evalue=8e-12,
Organism=Escherichia coli, GI1789492, Length=277, Percent_Identity=27.0758122743682, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33888; Mature: 33888

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKYPSLDDLNVFVHVARRSSFVGAADELGMSAAYVSKRIRLLEQGMGVVLLHRSTRQVS
CCCCCCCCCCHHEEEEEHHCCCCCCHHHHCHHHHHHHHHHHHHHCCCCEEEEECCCCEEE
ITEDGERVYEWAKGMLDSVQQMGDEVAALHGEPSGLLRVVSSQGFGRRFVAPALSELAAR
ECCCHHHHHHHHHHHHHHHHHHHHHHEECCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHH
YPKLDIRLDIQDRLVDLIEEGVDLDIRVGNEIAPHLRARHLADNWRVLCASPGYLDVHGT
CCCEEEEECHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHCCCCEEEEECCCEEEECCC
PLNLGELANHDCLVIKERDRPFGIWHLNGPQGAETVKVTGSLSTNHGEIARQWCLDGRGV
CCCHHHHCCCCEEEEEECCCCEEEEECCCCCCCCEEEEEECCCCCCHHHHHHHHCCCCHH
LLRSLWDVRTDLAEGRLVQVLPDYRQDADIWAVHTSPLMSSAKVRVTVEFLRDYLALHPS
HHHHHHHHHHHHHCCCEEEECCCCCCCCCEEEEECCCCCCCCEEEEEHHHHHHHHHCCCC
PVNH
CCCC
>Mature Secondary Structure
MNKYPSLDDLNVFVHVARRSSFVGAADELGMSAAYVSKRIRLLEQGMGVVLLHRSTRQVS
CCCCCCCCCCHHEEEEEHHCCCCCCHHHHCHHHHHHHHHHHHHHCCCCEEEEECCCCEEE
ITEDGERVYEWAKGMLDSVQQMGDEVAALHGEPSGLLRVVSSQGFGRRFVAPALSELAAR
ECCCHHHHHHHHHHHHHHHHHHHHHHEECCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHH
YPKLDIRLDIQDRLVDLIEEGVDLDIRVGNEIAPHLRARHLADNWRVLCASPGYLDVHGT
CCCEEEEECHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHCCCCEEEEECCCEEEECCC
PLNLGELANHDCLVIKERDRPFGIWHLNGPQGAETVKVTGSLSTNHGEIARQWCLDGRGV
CCCHHHHCCCCEEEEEECCCCEEEEECCCCCCCCEEEEEECCCCCCHHHHHHHHCCCCHH
LLRSLWDVRTDLAEGRLVQVLPDYRQDADIWAVHTSPLMSSAKVRVTVEFLRDYLALHPS
HHHHHHHHHHHHHCCCEEEECCCCCCCCCEEEEECCCCCCCCEEEEEHHHHHHHHHCCCC
PVNH
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097040; 9278503 [H]