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The map label for this gene is yneJ [H]

Identifier: 66045631

GI number: 66045631

Start: 2777182

End: 2778057

Strand: Direct

Name: yneJ [H]

Synonym: Psyr_2395

Alternate gene names: 66045631

Gene position: 2777182-2778057 (Clockwise)

Preceding gene: 66045629

Following gene: 66045633

Centisome position: 45.57

GC content: 63.58

Gene sequence:

>876_bases
ATGGATCTGGTTCAGCTGGAGATCTTCAAGGCAGTGGCCGAACACGGCAGCATCAGCGCCGCCGCGCAGCACATCCACCG
GGTGCCCTCGAACCTGACCACGCGCATCAAGCAGCTGGAGCAGGATCTGGGCGTCGACCTGTTCATTCGCGAGAAGCACC
GCTTGCGCCTGTCTCCTGCAGGCTGGAATTTTCTCGACTACACGCGACGCATCCTGGATCTGGTGCAGGAAGCGCGTTCT
GCCGTGTCCGGGGAGGAGCCACAGGGGCCTTTTGCACTGGGGTCGCTGGAAAGCACGGCTGCGGTCAGGATTCCTGCGCT
GCTGGCCGCCTACAACCAGCAGAACGCCAAGGTCGAGCTGGACCTGTCCACCGGCCCGTCCGGAACCATGATCGACGGCG
TGCTGGCCGGTAGACTGGTCGCTGCGTTCGTCGACGGGCCGGTGCAGCATCCATCGCTCGACGGCGTGCCGGTCTTCGAA
GAGGAAATGGTCATCATCGCGCCGCTCAATCATGCGCCGGTCAAGCGCGGGCGGGATGTCAGCGGAGAGAGTATTTATGC
GTTTCGTGCCAACTGCTCGTACCGCCATCACTTTGAACGCTGGTTCACCGATGACGCGGCAGTGCCGGGCAAGATCCATG
AAATGGAGTCCTACCACGGCATGCTCGCCTGCGTCAGCGCCGGTGCCGGGCTGGCGCTGATGCCGCGCAGCATGCTGGAA
AGCATGCCCGGCTGCGCCACGGTCAGTATCTGGCCGTTGTCCGAGAAGTTTCGCTACCTGCACACCTGGCTGATCTGGCG
CCGGGGCACGGTGTCGCGCAGCCTGACCCGCTTTGTCGAGCTGCTGGAACAGCGCAGCGAACCGGCGAACGTTTAA

Upstream 100 bases:

>100_bases
GCGCCATCCTGTTGAGGTGGAAGTGGGACCAGAATATAGGCGTGCAATGCTTTCTAAAACTGAATAATAATGAGCGTAAC
GTTCATGTTTGGAGAATTAT

Downstream 100 bases:

>100_bases
TGGCCTGCAGAAACCCGCTCGGCAATCGCCGCACCCACGTGCTGAGTCGAAAGCGTGCCGCCCAGATCCGGCGTGACGTC
GCCGTTGGCGATCACTTGCT

Product: regulatory protein, LysR:LysR, substrate-binding

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 291; Mature: 291

Protein sequence:

>291_residues
MDLVQLEIFKAVAEHGSISAAAQHIHRVPSNLTTRIKQLEQDLGVDLFIREKHRLRLSPAGWNFLDYTRRILDLVQEARS
AVSGEEPQGPFALGSLESTAAVRIPALLAAYNQQNAKVELDLSTGPSGTMIDGVLAGRLVAAFVDGPVQHPSLDGVPVFE
EEMVIIAPLNHAPVKRGRDVSGESIYAFRANCSYRHHFERWFTDDAAVPGKIHEMESYHGMLACVSAGAGLALMPRSMLE
SMPGCATVSIWPLSEKFRYLHTWLIWRRGTVSRSLTRFVELLEQRSEPANV

Sequences:

>Translated_291_residues
MDLVQLEIFKAVAEHGSISAAAQHIHRVPSNLTTRIKQLEQDLGVDLFIREKHRLRLSPAGWNFLDYTRRILDLVQEARS
AVSGEEPQGPFALGSLESTAAVRIPALLAAYNQQNAKVELDLSTGPSGTMIDGVLAGRLVAAFVDGPVQHPSLDGVPVFE
EEMVIIAPLNHAPVKRGRDVSGESIYAFRANCSYRHHFERWFTDDAAVPGKIHEMESYHGMLACVSAGAGLALMPRSMLE
SMPGCATVSIWPLSEKFRYLHTWLIWRRGTVSRSLTRFVELLEQRSEPANV
>Mature_291_residues
MDLVQLEIFKAVAEHGSISAAAQHIHRVPSNLTTRIKQLEQDLGVDLFIREKHRLRLSPAGWNFLDYTRRILDLVQEARS
AVSGEEPQGPFALGSLESTAAVRIPALLAAYNQQNAKVELDLSTGPSGTMIDGVLAGRLVAAFVDGPVQHPSLDGVPVFE
EEMVIIAPLNHAPVKRGRDVSGESIYAFRANCSYRHHFERWFTDDAAVPGKIHEMESYHGMLACVSAGAGLALMPRSMLE
SMPGCATVSIWPLSEKFRYLHTWLIWRRGTVSRSLTRFVELLEQRSEPANV

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787806, Length=283, Percent_Identity=66.4310954063604, Blast_Score=403, Evalue=1e-114,
Organism=Escherichia coli, GI1788887, Length=295, Percent_Identity=21.6949152542373, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1790208, Length=254, Percent_Identity=27.1653543307087, Blast_Score=81, Evalue=9e-17,
Organism=Escherichia coli, GI87082132, Length=291, Percent_Identity=24.7422680412371, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI157672245, Length=255, Percent_Identity=23.1372549019608, Blast_Score=71, Evalue=6e-14,
Organism=Escherichia coli, GI1790262, Length=248, Percent_Identity=24.1935483870968, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1787601, Length=285, Percent_Identity=23.5087719298246, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1788481, Length=298, Percent_Identity=25.8389261744966, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1790399, Length=241, Percent_Identity=23.6514522821577, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI145693105, Length=288, Percent_Identity=22.2222222222222, Blast_Score=66, Evalue=3e-12,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32235; Mature: 32235

Theoretical pI: Translated: 6.74; Mature: 6.74

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLVQLEIFKAVAEHGSISAAAQHIHRVPSNLTTRIKQLEQDLGVDLFIREKHRLRLSPA
CCCHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHHHCCCEEEEECCCCEEECCC
GWNFLDYTRRILDLVQEARSAVSGEEPQGPFALGSLESTAAVRIPALLAAYNQQNAKVEL
CCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCHHHHHHHHHHHHCCCCCEEEE
DLSTGPSGTMIDGVLAGRLVAAFVDGPVQHPSLDGVPVFEEEMVIIAPLNHAPVKRGRDV
EECCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCC
SGESIYAFRANCSYRHHFERWFTDDAAVPGKIHEMESYHGMLACVSAGAGLALMPRSMLE
CCCEEEEEECCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEECCHHHHH
SMPGCATVSIWPLSEKFRYLHTWLIWRRGTVSRSLTRFVELLEQRSEPANV
HCCCCEEEEEECCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MDLVQLEIFKAVAEHGSISAAAQHIHRVPSNLTTRIKQLEQDLGVDLFIREKHRLRLSPA
CCCHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHHHCCCEEEEECCCCEEECCC
GWNFLDYTRRILDLVQEARSAVSGEEPQGPFALGSLESTAAVRIPALLAAYNQQNAKVEL
CCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCHHHHHHHHHHHHCCCCCEEEE
DLSTGPSGTMIDGVLAGRLVAAFVDGPVQHPSLDGVPVFEEEMVIIAPLNHAPVKRGRDV
EECCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCC
SGESIYAFRANCSYRHHFERWFTDDAAVPGKIHEMESYHGMLACVSAGAGLALMPRSMLE
CCCEEEEEECCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEECCHHHHH
SMPGCATVSIWPLSEKFRYLHTWLIWRRGTVSRSLTRFVELLEQRSEPANV
HCCCCEEEEEECCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]