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The map label for this gene is pcpB [H]

Identifier: 66045613

GI number: 66045613

Start: 2755818

End: 2757479

Strand: Reverse

Name: pcpB [H]

Synonym: Psyr_2377

Alternate gene names: 66045613

Gene position: 2757479-2755818 (Counterclockwise)

Preceding gene: 66045615

Following gene: 66045612

Centisome position: 45.25

GC content: 59.63

Gene sequence:

>1662_bases
ATGTCAGACGGCGATATTCATTTCCAGTCAAACACCTTTAAATTGAATACACATCAGCCTGTTGAGCAGGACGTTTGCGA
CGTCCTGATAATCGGCTCGGGGCCGACCGGACTGGCGCTGGCCTGCGATCTGGCCAGGCGCGGGATCAAGGTCAGAATCA
TTGAAAAATCGGCTGTCCCCTTCAATGGTTCTCGCGGCAAAGGCATACAGCCACGAACGATCGAGGTGTTCGATAACCTT
GGCGTCGCCCAGCCATTGTTGGGCGTCGGGCAGCTTTATCCGCCGATGAAGTTTCATGTTTCTTTCCTGAAGATCAAATG
GAACATGATGAAGTACCAGAAACAGACAGCGGATATCCCGTACCCGAACGTCTGGCTCGTACCGCAATGGCGCACAGAGC
AGGTGTTGCGTGATCGACTGGCTGAACTGGGCACTCAGGTTGAATGGGACACTGGAGCACTCCAGATCAAACAGGATGCC
GAAGGTGTCTCTGTTCGGGTTGCTTGTCAGGGAGAGCCTCGTATCGTGCATGCCCGCTATCTGGTCGGCGCTGACGGCGG
CAAGAGTTTTGTGCGCAAGCAGCTGGGCGTGAACTTCACGGGCTCCACGTCTCAGGAAGGCCGGATGATCGTTGGCGATC
TGCACGTAGAGGGGCTTTCCCGGGATGCATGGCACATATGGCCGACGCGCAAAGGTGGAATGATCGGCCTGTGTCCGCTT
CCGCATTCGAGCCTGTTCCAGCTGATGATGCGTCTGGACGCCGACGAACCCGCTCCGGAACTCTCAGAGCATGCTATCCA
GACCCGCTGGCTGGCAGCTACGGGCTCCAGGAAGATTCACCTGCACAGCCCGACCTGGCTTTCGGTATTCCGCCCCAATG
TTCGGCTGGCCGAGCGCTATCGGGTCGAGCGGGTGTTCCTCGCCGGCGACGCCGCCCATGTGCATACCCCCGCAGGTGCT
CAAGGCCTCAACACGGGCGTGCAGGACGCCTGGAATCTGGGCTGGAAACTGGCAGCGGTGCTGGACGGCGCGCCCGAGGC
ACTGCTCGACACTTACGAAGAGGAACGCAGGCCTGTAGCTGCTGCGGTGCTGGAGTTGTCCAGCGAACTCTTCAATAACA
CCACTGGCACCGGCTTGCCCACATTCAGGCGTGGCGACAAGGAGCGGCAGTTATTACTCAACTACCGCGCCAGCTCGCTT
TCCGTGAACAGCGCCGACAACGTCGATGGCAAGGTCCAGGCAGGTGATCGCGCACCCGATGCCACCTGCTCGCAGCAGCA
CGGCGGACGCACACGATTGTTCGACGTTTTCAGAGGTGGGCATTTCACCTTGCTGGCCTTCGGTACGCAGGCCATCGCTG
CAATGAATACTTTCGGCAGCCGCGACGAGCGATTCCTGCAGGCAGTTGCGGTTCGCCCCGCGCACCTCAGTAATGACCGC
TACGGTCTGGTCGATGAAACCGGTCAGGCACGTGACGCTTACGGCGTAAGCCTTCAGGAAAACATCATCTTTCTGATACG
TCCCGACGGGTACGTCGGCCTGACCGCCACGGACGATTTCGTACCGGCAGTCCAGCGTTACCTGGCCACCCTGTCCCGCA
CACCTGACTCTCTCGCTGGCAGCGACGCCGACATGCAGGCGACACGCGCAGTCGCACACTGA

Upstream 100 bases:

>100_bases
TGATTTATTGACACAACCCTATTTCGGATACATCGTATCCGAAATAGGAGAGACAACCATCTGCCGCTGGAAGTGGCCTA
CACAAAAGTGGAGTTCAGGT

Downstream 100 bases:

>100_bases
TGCACGCCAGAAAGACCACTCAGAGAGCCTCGACATGAAGCACCTTTTCCTTCCTGCACTGACTGTTGTCGTATTGCCTG
CCCTGTCTTTACAGCTGGCC

Product: hypothetical protein

Products: NA

Alternate protein names: Pentachlorophenol hydroxylase [H]

Number of amino acids: Translated: 553; Mature: 552

Protein sequence:

>553_residues
MSDGDIHFQSNTFKLNTHQPVEQDVCDVLIIGSGPTGLALACDLARRGIKVRIIEKSAVPFNGSRGKGIQPRTIEVFDNL
GVAQPLLGVGQLYPPMKFHVSFLKIKWNMMKYQKQTADIPYPNVWLVPQWRTEQVLRDRLAELGTQVEWDTGALQIKQDA
EGVSVRVACQGEPRIVHARYLVGADGGKSFVRKQLGVNFTGSTSQEGRMIVGDLHVEGLSRDAWHIWPTRKGGMIGLCPL
PHSSLFQLMMRLDADEPAPELSEHAIQTRWLAATGSRKIHLHSPTWLSVFRPNVRLAERYRVERVFLAGDAAHVHTPAGA
QGLNTGVQDAWNLGWKLAAVLDGAPEALLDTYEEERRPVAAAVLELSSELFNNTTGTGLPTFRRGDKERQLLLNYRASSL
SVNSADNVDGKVQAGDRAPDATCSQQHGGRTRLFDVFRGGHFTLLAFGTQAIAAMNTFGSRDERFLQAVAVRPAHLSNDR
YGLVDETGQARDAYGVSLQENIIFLIRPDGYVGLTATDDFVPAVQRYLATLSRTPDSLAGSDADMQATRAVAH

Sequences:

>Translated_553_residues
MSDGDIHFQSNTFKLNTHQPVEQDVCDVLIIGSGPTGLALACDLARRGIKVRIIEKSAVPFNGSRGKGIQPRTIEVFDNL
GVAQPLLGVGQLYPPMKFHVSFLKIKWNMMKYQKQTADIPYPNVWLVPQWRTEQVLRDRLAELGTQVEWDTGALQIKQDA
EGVSVRVACQGEPRIVHARYLVGADGGKSFVRKQLGVNFTGSTSQEGRMIVGDLHVEGLSRDAWHIWPTRKGGMIGLCPL
PHSSLFQLMMRLDADEPAPELSEHAIQTRWLAATGSRKIHLHSPTWLSVFRPNVRLAERYRVERVFLAGDAAHVHTPAGA
QGLNTGVQDAWNLGWKLAAVLDGAPEALLDTYEEERRPVAAAVLELSSELFNNTTGTGLPTFRRGDKERQLLLNYRASSL
SVNSADNVDGKVQAGDRAPDATCSQQHGGRTRLFDVFRGGHFTLLAFGTQAIAAMNTFGSRDERFLQAVAVRPAHLSNDR
YGLVDETGQARDAYGVSLQENIIFLIRPDGYVGLTATDDFVPAVQRYLATLSRTPDSLAGSDADMQATRAVAH
>Mature_552_residues
SDGDIHFQSNTFKLNTHQPVEQDVCDVLIIGSGPTGLALACDLARRGIKVRIIEKSAVPFNGSRGKGIQPRTIEVFDNLG
VAQPLLGVGQLYPPMKFHVSFLKIKWNMMKYQKQTADIPYPNVWLVPQWRTEQVLRDRLAELGTQVEWDTGALQIKQDAE
GVSVRVACQGEPRIVHARYLVGADGGKSFVRKQLGVNFTGSTSQEGRMIVGDLHVEGLSRDAWHIWPTRKGGMIGLCPLP
HSSLFQLMMRLDADEPAPELSEHAIQTRWLAATGSRKIHLHSPTWLSVFRPNVRLAERYRVERVFLAGDAAHVHTPAGAQ
GLNTGVQDAWNLGWKLAAVLDGAPEALLDTYEEERRPVAAAVLELSSELFNNTTGTGLPTFRRGDKERQLLLNYRASSLS
VNSADNVDGKVQAGDRAPDATCSQQHGGRTRLFDVFRGGHFTLLAFGTQAIAAMNTFGSRDERFLQAVAVRPAHLSNDRY
GLVDETGQARDAYGVSLQENIIFLIRPDGYVGLTATDDFVPAVQRYLATLSRTPDSLAGSDADMQATRAVAH

Specific function: Dechlorination of pentachlorophenol to tetrachlorohydroquinone. Removes also hydrogen and nitro, amino, and cyano groups from benzene ring at the para position in relation to the hydroxyl of phenol [H]

COG id: COG0654

COG function: function code HC; 2-polyprenyl-6-methoxyphenol hydroxylase and related FAD-dependent oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pheA/tfdB FAD monooxygenase family [H]

Homologues:

Organism=Escherichia coli, GI1786543, Length=572, Percent_Identity=27.2727272727273, Blast_Score=134, Evalue=2e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002938
- InterPro:   IPR003042 [H]

Pfam domain/function: PF01494 FAD_binding_3 [H]

EC number: =1.14.13.50 [H]

Molecular weight: Translated: 60818; Mature: 60686

Theoretical pI: Translated: 7.48; Mature: 7.48

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDGDIHFQSNTFKLNTHQPVEQDVCDVLIIGSGPTGLALACDLARRGIKVRIIEKSAVP
CCCCCEEEECCEEEECCCCCCCCCCCCEEEECCCCCCHHEEEHHHHCCCEEEEEECCCCC
FNGSRGKGIQPRTIEVFDNLGVAQPLLGVGQLYPPMKFHVSFLKIKWNMMKYQKQTADIP
CCCCCCCCCCCCEEEEECCCCHHHHHHHHHHCCCCHHHEEEEEEEEEHHHHHHHHHCCCC
YPNVWLVPQWRTEQVLRDRLAELGTQVEWDTGALQIKQDAEGVSVRVACQGEPRIVHARY
CCCEEEECCCCHHHHHHHHHHHHCCEEEECCCCEEEEECCCCEEEEEEECCCCEEEEEEE
LVGADGGKSFVRKQLGVNFTGSTSQEGRMIVGDLHVEGLSRDAWHIWPTRKGGMIGLCPL
EEECCCCHHHHHHHHCCCCCCCCCCCCCEEEEEEEECCCCCCCEEEEECCCCCEEEECCC
PHSSLFQLMMRLDADEPAPELSEHAIQTRWLAATGSRKIHLHSPTWLSVFRPNVRLAERY
CHHHHHHHHHHHCCCCCCCHHHHHHHHHEEEEECCCCEEEEECCCEEEEECCCCHHHHHH
RVERVFLAGDAAHVHTPAGAQGLNTGVQDAWNLGWKLAAVLDGAPEALLDTYEEERRPVA
CEEEEEEECCCCEEECCCCCCCCCCCHHHHHCCCEEEEEECCCCCHHHHHHHHHHCCHHH
AAVLELSSELFNNTTGTGLPTFRRGDKERQLLLNYRASSLSVNSADNVDGKVQAGDRAPD
HHHHHHHHHHHCCCCCCCCCCCCCCCHHHEEEEEECCCEEECCCCCCCCCEEECCCCCCC
ATCSQQHGGRTRLFDVFRGGHFTLLAFGTQAIAAMNTFGSRDERFLQAVAVRPAHLSNDR
CCCHHCCCCCEEEEHHHCCCCEEEEEECHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCC
YGLVDETGQARDAYGVSLQENIIFLIRPDGYVGLTATDDFVPAVQRYLATLSRTPDSLAG
CCCCCCCCCCCCCCCCEEECCEEEEECCCCEEEEEECCHHHHHHHHHHHHHHCCCHHHCC
SDADMQATRAVAH
CCCCHHHHHHHCC
>Mature Secondary Structure 
SDGDIHFQSNTFKLNTHQPVEQDVCDVLIIGSGPTGLALACDLARRGIKVRIIEKSAVP
CCCCEEEECCEEEECCCCCCCCCCCCEEEECCCCCCHHEEEHHHHCCCEEEEEECCCCC
FNGSRGKGIQPRTIEVFDNLGVAQPLLGVGQLYPPMKFHVSFLKIKWNMMKYQKQTADIP
CCCCCCCCCCCCEEEEECCCCHHHHHHHHHHCCCCHHHEEEEEEEEEHHHHHHHHHCCCC
YPNVWLVPQWRTEQVLRDRLAELGTQVEWDTGALQIKQDAEGVSVRVACQGEPRIVHARY
CCCEEEECCCCHHHHHHHHHHHHCCEEEECCCCEEEEECCCCEEEEEEECCCCEEEEEEE
LVGADGGKSFVRKQLGVNFTGSTSQEGRMIVGDLHVEGLSRDAWHIWPTRKGGMIGLCPL
EEECCCCHHHHHHHHCCCCCCCCCCCCCEEEEEEEECCCCCCCEEEEECCCCCEEEECCC
PHSSLFQLMMRLDADEPAPELSEHAIQTRWLAATGSRKIHLHSPTWLSVFRPNVRLAERY
CHHHHHHHHHHHCCCCCCCHHHHHHHHHEEEEECCCCEEEEECCCEEEEECCCCHHHHHH
RVERVFLAGDAAHVHTPAGAQGLNTGVQDAWNLGWKLAAVLDGAPEALLDTYEEERRPVA
CEEEEEEECCCCEEECCCCCCCCCCCHHHHHCCCEEEEEECCCCCHHHHHHHHHHCCHHH
AAVLELSSELFNNTTGTGLPTFRRGDKERQLLLNYRASSLSVNSADNVDGKVQAGDRAPD
HHHHHHHHHHHCCCCCCCCCCCCCCCHHHEEEEEECCCEEECCCCCCCCCEEECCCCCCC
ATCSQQHGGRTRLFDVFRGGHFTLLAFGTQAIAAMNTFGSRDERFLQAVAVRPAHLSNDR
CCCHHCCCCCEEEEHHHCCCCEEEEEECHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCC
YGLVDETGQARDAYGVSLQENIIFLIRPDGYVGLTATDDFVPAVQRYLATLSRTPDSLAG
CCCCCCCCCCCCCCCCEEECCEEEEECCCCEEEEEECCHHHHHHHHHHHHHHCCCHHHCC
SDADMQATRAVAH
CCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7678243; 12169590 [H]