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The map label for this gene is potB [H]

Identifier: 66045571

GI number: 66045571

Start: 2711598

End: 2712470

Strand: Reverse

Name: potB [H]

Synonym: Psyr_2334

Alternate gene names: 66045571

Gene position: 2712470-2711598 (Counterclockwise)

Preceding gene: 66045572

Following gene: 66045570

Centisome position: 44.51

GC content: 61.86

Gene sequence:

>873_bases
ATGAGCCTGCTGAGCGAAATGCGTCAGGGCGGCCGCGGCTATTGGCTGTCAGCTCCGGCGCTGGCGTTGTATATCGGCTT
GCTGGTGCTGCCGCTGGGTCTGACCCTGGTGCTGTCGTTCAACGTGTTCGACTATCAGGTCGGGGTGAAAAGCGACAGCT
ACACGCTGGCCAACTATGTGGCCGTGGTCACCGACTCGTATTTCTATGAAATCTTCCTGCGCACCTTCTGGATCAGTGCG
CTGGTCACCCTGCTGTGCGTGCTGATCGGCGTCCCCGAAGCCTACATTCTCAGCCGCATGGGCACGCCGTGGCGCTCGAT
CTTCCTGATCCTGATTCTCACGCCGCTGCTGATCTCGGTGGTGGTCCGGGCGTTCGGCTGGAGCCTGCTGCTGGGCGCGG
ACGGTCTGATCAATCAGGTCATCCAGTTCATGGGCGGTCGCCCGGTGAAACTGCTGTACACCCCGTTTGCCGTGGTCATT
GCGCTAGTCCACGTCATGTTGCCGTTCATGATCATTCCGGTCTGGACCTCGCTGCAAAAGCTCGACCCCACCGCCGAGCA
GGCCGCGCTGTCGCTGGGCGCCAGCCAGGCGAAAGTCATGCGCCTGATCGTGCTGCCGCAGGTCATGCCCGGCGTGCTGT
CCGGCTCGCTGATCGTGTTCGGCCTGGCCGCCAGTTCGTTCGCCATTCCCGGCCTGCTCGGCGGACGACGTCTGAAGATG
GTCGCCACGGTGATCTACGACCAGTACCTCTCGGAGTTGAACTGGCCGATGGGCGCGACCCTTGCCGTGGCGCTGCTGTT
GGTCAACCTTCTGGTCATGCTGTCCTGGAACCGCATGATCGAAGGCCGTTACAAAAAAACTCTGGGGGAATGA

Upstream 100 bases:

>100_bases
GTCGCAACGACGGCCAGGCGCCGCTGGAACAAGGCGCGGCGGTCGACATGGACTGGCCCGACGAGTTGCTGCGCGTGCTG
GATGCCGACGAGGTGCGCGC

Downstream 100 bases:

>100_bases
TCCATGTCCAAGAACGGTCCGTTGGCCCTGTCGTTTCATGCGCTGGTGGTGATCTTCATGATGGCCCCGCTGGTGGTGGT
GTGCCTGGTGGCGTTCACCC

Product: binding-protein dependent transport system inner membrane protein

Products: ADP; phosphate; putrescine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 290; Mature: 289

Protein sequence:

>290_residues
MSLLSEMRQGGRGYWLSAPALALYIGLLVLPLGLTLVLSFNVFDYQVGVKSDSYTLANYVAVVTDSYFYEIFLRTFWISA
LVTLLCVLIGVPEAYILSRMGTPWRSIFLILILTPLLISVVVRAFGWSLLLGADGLINQVIQFMGGRPVKLLYTPFAVVI
ALVHVMLPFMIIPVWTSLQKLDPTAEQAALSLGASQAKVMRLIVLPQVMPGVLSGSLIVFGLAASSFAIPGLLGGRRLKM
VATVIYDQYLSELNWPMGATLAVALLLVNLLVMLSWNRMIEGRYKKTLGE

Sequences:

>Translated_290_residues
MSLLSEMRQGGRGYWLSAPALALYIGLLVLPLGLTLVLSFNVFDYQVGVKSDSYTLANYVAVVTDSYFYEIFLRTFWISA
LVTLLCVLIGVPEAYILSRMGTPWRSIFLILILTPLLISVVVRAFGWSLLLGADGLINQVIQFMGGRPVKLLYTPFAVVI
ALVHVMLPFMIIPVWTSLQKLDPTAEQAALSLGASQAKVMRLIVLPQVMPGVLSGSLIVFGLAASSFAIPGLLGGRRLKM
VATVIYDQYLSELNWPMGATLAVALLLVNLLVMLSWNRMIEGRYKKTLGE
>Mature_289_residues
SLLSEMRQGGRGYWLSAPALALYIGLLVLPLGLTLVLSFNVFDYQVGVKSDSYTLANYVAVVTDSYFYEIFLRTFWISAL
VTLLCVLIGVPEAYILSRMGTPWRSIFLILILTPLLISVVVRAFGWSLLLGADGLINQVIQFMGGRPVKLLYTPFAVVIA
LVHVMLPFMIIPVWTSLQKLDPTAEQAALSLGASQAKVMRLIVLPQVMPGVLSGSLIVFGLAASSFAIPGLLGGRRLKMV
ATVIYDQYLSELNWPMGATLAVALLLVNLLVMLSWNRMIEGRYKKTLGE

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine [H]

COG id: COG1176

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component I

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787080, Length=252, Percent_Identity=29.3650793650794, Blast_Score=139, Evalue=2e-34,
Organism=Escherichia coli, GI1787369, Length=227, Percent_Identity=32.15859030837, Blast_Score=139, Evalue=2e-34,
Organism=Escherichia coli, GI1787713, Length=236, Percent_Identity=29.2372881355932, Blast_Score=103, Evalue=1e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 31848; Mature: 31717

Theoretical pI: Translated: 9.72; Mature: 9.72

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLLSEMRQGGRGYWLSAPALALYIGLLVLPLGLTLVLSFNVFDYQVGVKSDSYTLANYV
CCHHHHHHHCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCHHHHHHH
AVVTDSYFYEIFLRTFWISALVTLLCVLIGVPEAYILSRMGTPWRSIFLILILTPLLISV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHH
VVRAFGWSLLLGADGLINQVIQFMGGRPVKLLYTPFAVVIALVHVMLPFMIIPVWTSLQK
HHHHHCHHHHHCCCHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LDPTAEQAALSLGASQAKVMRLIVLPQVMPGVLSGSLIVFGLAASSFAIPGLLGGRRLKM
CCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCHHHHH
VATVIYDQYLSELNWPMGATLAVALLLVNLLVMLSWNRMIEGRYKKTLGE
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SLLSEMRQGGRGYWLSAPALALYIGLLVLPLGLTLVLSFNVFDYQVGVKSDSYTLANYV
CHHHHHHHCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCHHHHHHH
AVVTDSYFYEIFLRTFWISALVTLLCVLIGVPEAYILSRMGTPWRSIFLILILTPLLISV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHH
VVRAFGWSLLLGADGLINQVIQFMGGRPVKLLYTPFAVVIALVHVMLPFMIIPVWTSLQK
HHHHHCHHHHHCCCHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LDPTAEQAALSLGASQAKVMRLIVLPQVMPGVLSGSLIVFGLAASSFAIPGLLGGRRLKM
CCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCHHHHH
VATVIYDQYLSELNWPMGATLAVALLLVNLLVMLSWNRMIEGRYKKTLGE
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; putrescine [Periplasm]; H2O [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]