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The map label for this gene is gatA2 [H]

Identifier: 66045500

GI number: 66045500

Start: 2624092

End: 2625615

Strand: Reverse

Name: gatA2 [H]

Synonym: Psyr_2260

Alternate gene names: 66045500

Gene position: 2625615-2624092 (Counterclockwise)

Preceding gene: 66045501

Following gene: 66045485

Centisome position: 43.09

GC content: 63.58

Gene sequence:

>1524_bases
ATGCAAAACTCGTCCGAGCTGCTCGGTAAATCCGCCACCGAACTGCGTGCGCTGATCGGCAACAAACAGCTCTCTCCGGT
GGAGTTGCTGGACGCCTGTATCGAGCGGATTGAAAGCCTGAACCCGAAGATCAATGCCTTCGCCGCAACCTGTTTCGAGC
GCGCCCGCGACGAGGCACTGCTGGCCGAACAGGACGTTATGCAAGGCAAGCCGCTGGGGCTGCTGCATGGCCTGCCAATC
GGCATCAAGGACCTGGAAGAAACCGCCGGGGTCCTCACCACCTACGGCTCGCAACTGTTTCGCGACAACATTCCCGCGCA
GGACAACCTGTTCGTCGCGCGCCTGCGTGCGGCCGGGGCAATAATGGTCGGCAAGACCAATGTTCCGGAGCTGGGCGCAG
GCGCCAATACCCGCAATGTGGTGTGGGGCGCGACCGGTAACCCGTTCAATCCCGAGCTGAATGCCGGCGGTTCCTCGGGC
GGATCAGCCGCCGCACTGGCCGTGGACATGGTGCCGTTGTGCAGCGGCTCGGACACCGGCGGTTCGCTGCGCATTCCCGC
GGCGCTGTGCGGTATCGTCGGCCTGCGTCCTTCGCCCGGGCTGGTGCCGAGTGAGCGCAAAAAGCTCGGCTGGACACCGA
TTTCAGTGGTAGGCCCCATGGGCCGCAATGTTGCCGATACCCTGCTGCAACTGCGCGCCAGCGCCGGGTTGGGGCAGTCC
GACCCGTTGAGTTATACCATCGCCGCCGACGAGTTCGCACCGCGCCCGATCGACCTCAGCCAGTTGCGCGTCGGCTACAG
CGAAGACTTCGGCGCGTGTGCTGTGGATGACAGAATCCGTGCCGTCTTTCGGGAAAAGATCAGCGCCCTGAAATCGCTGT
TCAAGTCCTGCGAAGCCATCGACCTGAACCTGACCAGCGCCCATCGCACCTTCGACGTCCTGCGTGCCGAAGCCTTCGTC
GCCGGGCTGCAGGACGCTCACGACTGCGATCCTGATGCACTGGGGCCCAACACCCGCGCCAATTTCGACATGGGTGCCGC
GATGTCTTTGCAGGACTGCGTCAAGGCGCATGGCGAGCAAAGCCGGATCTTTCGCGGTTTTCAGAAACAATTCGAGGAAT
ACGACCTGATCCTCGCGCCCACTACGCCAGTCTCGCCCTTCCCCTGGAGCGAGCTGTACCTGCGCGAAGTCAACGGCGTG
CCCCTGGACAATTACTACCGCTGGCTGGCGCTGTGCTACACCATCACCCTGACCACCAACCCGGCGCTGTCCCTGCCCTG
CGGCACCGACCACAACGGCATGCCGTTCGGGCTGCAGGTGATCGGCGGATTTCGTGGCGATGCGAAACTGCTGGCCTGCG
CTGCAGCGATCGAGCAGGCAACACTGAATGATCCACGCCTGTCACGTCCGCGTCCCGACCTGCAGAAACTGCTGGCCTCG
GCGGTCGACCTGACCCACATCGTCACGCATCCGCCGATTTATGGCGGGTCGAGAACGGGCAAGCCGGAGATCGGGGCGAT
GTGA

Upstream 100 bases:

>100_bases
GCATTCCCACGCGGAGCGTGCGGAACGATGAACATTCGGGCCAGTATCGTACCCATACTCCGCACACAGCGTTATACCTA
AGTCCAGCGAGGAACACTCC

Downstream 100 bases:

>100_bases
AGACGTCGCTCAGACCACCGTCATACCATCCCTCCCCACCACTACATTGCCCGGCAACTGTTTGGAGTGCTCCATCAACC
ACGCATCCAGCGTATGGCCG

Product: amidase

Products: NA

Alternate protein names: Glu-ADT subunit A 2 [H]

Number of amino acids: Translated: 507; Mature: 507

Protein sequence:

>507_residues
MQNSSELLGKSATELRALIGNKQLSPVELLDACIERIESLNPKINAFAATCFERARDEALLAEQDVMQGKPLGLLHGLPI
GIKDLEETAGVLTTYGSQLFRDNIPAQDNLFVARLRAAGAIMVGKTNVPELGAGANTRNVVWGATGNPFNPELNAGGSSG
GSAAALAVDMVPLCSGSDTGGSLRIPAALCGIVGLRPSPGLVPSERKKLGWTPISVVGPMGRNVADTLLQLRASAGLGQS
DPLSYTIAADEFAPRPIDLSQLRVGYSEDFGACAVDDRIRAVFREKISALKSLFKSCEAIDLNLTSAHRTFDVLRAEAFV
AGLQDAHDCDPDALGPNTRANFDMGAAMSLQDCVKAHGEQSRIFRGFQKQFEEYDLILAPTTPVSPFPWSELYLREVNGV
PLDNYYRWLALCYTITLTTNPALSLPCGTDHNGMPFGLQVIGGFRGDAKLLACAAAIEQATLNDPRLSRPRPDLQKLLAS
AVDLTHIVTHPPIYGGSRTGKPEIGAM

Sequences:

>Translated_507_residues
MQNSSELLGKSATELRALIGNKQLSPVELLDACIERIESLNPKINAFAATCFERARDEALLAEQDVMQGKPLGLLHGLPI
GIKDLEETAGVLTTYGSQLFRDNIPAQDNLFVARLRAAGAIMVGKTNVPELGAGANTRNVVWGATGNPFNPELNAGGSSG
GSAAALAVDMVPLCSGSDTGGSLRIPAALCGIVGLRPSPGLVPSERKKLGWTPISVVGPMGRNVADTLLQLRASAGLGQS
DPLSYTIAADEFAPRPIDLSQLRVGYSEDFGACAVDDRIRAVFREKISALKSLFKSCEAIDLNLTSAHRTFDVLRAEAFV
AGLQDAHDCDPDALGPNTRANFDMGAAMSLQDCVKAHGEQSRIFRGFQKQFEEYDLILAPTTPVSPFPWSELYLREVNGV
PLDNYYRWLALCYTITLTTNPALSLPCGTDHNGMPFGLQVIGGFRGDAKLLACAAAIEQATLNDPRLSRPRPDLQKLLAS
AVDLTHIVTHPPIYGGSRTGKPEIGAM
>Mature_507_residues
MQNSSELLGKSATELRALIGNKQLSPVELLDACIERIESLNPKINAFAATCFERARDEALLAEQDVMQGKPLGLLHGLPI
GIKDLEETAGVLTTYGSQLFRDNIPAQDNLFVARLRAAGAIMVGKTNVPELGAGANTRNVVWGATGNPFNPELNAGGSSG
GSAAALAVDMVPLCSGSDTGGSLRIPAALCGIVGLRPSPGLVPSERKKLGWTPISVVGPMGRNVADTLLQLRASAGLGQS
DPLSYTIAADEFAPRPIDLSQLRVGYSEDFGACAVDDRIRAVFREKISALKSLFKSCEAIDLNLTSAHRTFDVLRAEAFV
AGLQDAHDCDPDALGPNTRANFDMGAAMSLQDCVKAHGEQSRIFRGFQKQFEEYDLILAPTTPVSPFPWSELYLREVNGV
PLDNYYRWLALCYTITLTTNPALSLPCGTDHNGMPFGLQVIGGFRGDAKLLACAAAIEQATLNDPRLSRPRPDLQKLLAS
AVDLTHIVTHPPIYGGSRTGKPEIGAM

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=534, Percent_Identity=27.7153558052434, Blast_Score=139, Evalue=4e-33,
Organism=Homo sapiens, GI195972892, Length=491, Percent_Identity=25.6619144602851, Blast_Score=121, Evalue=2e-27,
Organism=Homo sapiens, GI166795287, Length=214, Percent_Identity=30.3738317757009, Blast_Score=69, Evalue=8e-12,
Organism=Caenorhabditis elegans, GI17537465, Length=501, Percent_Identity=27.1457085828343, Blast_Score=138, Evalue=6e-33,
Organism=Caenorhabditis elegans, GI17556264, Length=300, Percent_Identity=30.3333333333333, Blast_Score=99, Evalue=5e-21,
Organism=Caenorhabditis elegans, GI71990152, Length=307, Percent_Identity=29.3159609120521, Blast_Score=96, Evalue=6e-20,
Organism=Caenorhabditis elegans, GI17543272, Length=440, Percent_Identity=25.4545454545455, Blast_Score=92, Evalue=8e-19,
Organism=Caenorhabditis elegans, GI17538252, Length=185, Percent_Identity=28.6486486486486, Blast_Score=77, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17538254, Length=292, Percent_Identity=29.7945205479452, Blast_Score=72, Evalue=9e-13,
Organism=Saccharomyces cerevisiae, GI6319685, Length=392, Percent_Identity=27.0408163265306, Blast_Score=90, Evalue=7e-19,
Organism=Saccharomyces cerevisiae, GI6320448, Length=230, Percent_Identity=31.304347826087, Blast_Score=82, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6323950, Length=471, Percent_Identity=23.1422505307856, Blast_Score=74, Evalue=5e-14,
Organism=Drosophila melanogaster, GI24648435, Length=490, Percent_Identity=25.7142857142857, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI24648437, Length=490, Percent_Identity=25.7142857142857, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI45550774, Length=492, Percent_Identity=26.0162601626016, Blast_Score=124, Evalue=2e-28,
Organism=Drosophila melanogaster, GI21356731, Length=491, Percent_Identity=26.4765784114053, Blast_Score=122, Evalue=8e-28,
Organism=Drosophila melanogaster, GI24648113, Length=503, Percent_Identity=27.2365805168986, Blast_Score=115, Evalue=1e-25,
Organism=Drosophila melanogaster, GI161078093, Length=516, Percent_Identity=29.4573643410853, Blast_Score=113, Evalue=4e-25,
Organism=Drosophila melanogaster, GI24652985, Length=499, Percent_Identity=27.4549098196393, Blast_Score=108, Evalue=1e-23,
Organism=Drosophila melanogaster, GI19922090, Length=499, Percent_Identity=27.4549098196393, Blast_Score=108, Evalue=1e-23,
Organism=Drosophila melanogaster, GI24652981, Length=499, Percent_Identity=27.4549098196393, Blast_Score=108, Evalue=1e-23,
Organism=Drosophila melanogaster, GI24652983, Length=499, Percent_Identity=27.4549098196393, Blast_Score=108, Evalue=1e-23,
Organism=Drosophila melanogaster, GI24644968, Length=496, Percent_Identity=26.008064516129, Blast_Score=105, Evalue=6e-23,
Organism=Drosophila melanogaster, GI24648441, Length=415, Percent_Identity=25.0602409638554, Blast_Score=96, Evalue=6e-20,
Organism=Drosophila melanogaster, GI24648439, Length=415, Percent_Identity=25.0602409638554, Blast_Score=96, Evalue=6e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: NA

Molecular weight: Translated: 54077; Mature: 54077

Theoretical pI: Translated: 5.34; Mature: 5.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQNSSELLGKSATELRALIGNKQLSPVELLDACIERIESLNPKINAFAATCFERARDEAL
CCCCHHHHCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
LAEQDVMQGKPLGLLHGLPIGIKDLEETAGVLTTYGSQLFRDNIPAQDNLFVARLRAAGA
HHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHEEHHHCCE
IMVGKTNVPELGAGANTRNVVWGATGNPFNPELNAGGSSGGSAAALAVDMVPLCSGSDTG
EEECCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCEEEEEEHEECCCCCCCC
GSLRIPAALCGIVGLRPSPGLVPSERKKLGWTPISVVGPMGRNVADTLLQLRASAGLGQS
CCEECHHHHHHHHCCCCCCCCCCCHHHHCCCCCHHHCCCCCCHHHHHHHHHHHHCCCCCC
DPLSYTIAADEFAPRPIDLSQLRVGYSEDFGACAVDDRIRAVFREKISALKSLFKSCEAI
CCCEEEEECCCCCCCCCCHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEE
DLNLTSAHRTFDVLRAEAFVAGLQDAHDCDPDALGPNTRANFDMGAAMSLQDCVKAHGEQ
EEECCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCH
SRIFRGFQKQFEEYDLILAPTTPVSPFPWSELYLREVNGVPLDNYYRWLALCYTITLTTN
HHHHHHHHHHHHHCCEEEECCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHEEEEECC
PALSLPCGTDHNGMPFGLQVIGGFRGDAKLLACAAAIEQATLNDPRLSRPRPDLQKLLAS
CCEECCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHH
AVDLTHIVTHPPIYGGSRTGKPEIGAM
HHHHHHHHCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MQNSSELLGKSATELRALIGNKQLSPVELLDACIERIESLNPKINAFAATCFERARDEAL
CCCCHHHHCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
LAEQDVMQGKPLGLLHGLPIGIKDLEETAGVLTTYGSQLFRDNIPAQDNLFVARLRAAGA
HHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHEEHHHCCE
IMVGKTNVPELGAGANTRNVVWGATGNPFNPELNAGGSSGGSAAALAVDMVPLCSGSDTG
EEECCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCEEEEEEHEECCCCCCCC
GSLRIPAALCGIVGLRPSPGLVPSERKKLGWTPISVVGPMGRNVADTLLQLRASAGLGQS
CCEECHHHHHHHHCCCCCCCCCCCHHHHCCCCCHHHCCCCCCHHHHHHHHHHHHCCCCCC
DPLSYTIAADEFAPRPIDLSQLRVGYSEDFGACAVDDRIRAVFREKISALKSLFKSCEAI
CCCEEEEECCCCCCCCCCHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEE
DLNLTSAHRTFDVLRAEAFVAGLQDAHDCDPDALGPNTRANFDMGAAMSLQDCVKAHGEQ
EEECCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCH
SRIFRGFQKQFEEYDLILAPTTPVSPFPWSELYLREVNGVPLDNYYRWLALCYTITLTTN
HHHHHHHHHHHHHCCEEEECCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHEEEEECC
PALSLPCGTDHNGMPFGLQVIGGFRGDAKLLACAAAIEQATLNDPRLSRPRPDLQKLLAS
CCEECCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHH
AVDLTHIVTHPPIYGGSRTGKPEIGAM
HHHHHHHHCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11466286 [H]