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The map label for this gene is purU [H]

Identifier: 66045466

GI number: 66045466

Start: 2587752

End: 2588618

Strand: Reverse

Name: purU [H]

Synonym: Psyr_2226

Alternate gene names: 66045466

Gene position: 2588618-2587752 (Counterclockwise)

Preceding gene: 66045467

Following gene: 66045465

Centisome position: 42.48

GC content: 59.17

Gene sequence:

>867_bases
ATGCAACACGAGAAGAACCATTTCATCCTGAAGATCAGCTGCCCGGCGACCTCCGGCATTGTCGCGGCGGTGACTTCCTA
CCTGGCTGGCAACAGCTGCTACATCGGTGAAATGGCCCAGTTCGACGATGAGTACAGCGGCACCTTTTTCATGCGCGCCG
TGTTTCGTTTCAACGATGGTCATGAGGGTGACATCCAGCAGTTGAAAGCCGGCTTCGATGCCGTGGCCAGCGACTTTGCC
ATGCAGTGGGAGCTGCATGACACGCGCCGGCCGATGCGTGTGCTGCTGATGGTCAGCAAGTTCGATCACTGCCTGACCGA
CCTGCTGTACCGCTACCACAAGGGCGAAATGGACATGACCATCACCGCCATCGTCTCCAATCACCTGGATTTGCGACCGA
TGGCCGAACGCGAGGGCATTCGTTTCATTTACCTGCCCGTGACCAGGGAAACCAAGGCTGCACAGGAAGCCGCACTGATG
AAGGTGGTCGATGAAACCGGCACAGAGCTGGTGGTGCTGGCGCGCTACATGCAGATTCTCTCCGACGACCTGTGCCAGCA
ACTGGCGGGTCGGGCGATCAATATCCATCACTCGTTTCTGCCGGGTTTCAAGGGCGCCAAGCCCTACCATCAGGCTTACG
AACGCGGCGTGAAGCTGATCGGCGCCACCGCGCATTACGTCACCAGCGACCTGGACGAAGGCCCGATCATCGAGCAGGAA
GTGCAGCGCGTCGATCACGTCTACCTGCCTGCCGACCTGGTCGCGGCGGGACGCAATAACGAAACCATAGCGCTGTCGCG
GGCCGTCAAATACCACCTGGAGCACCGCGTGTTTCTCAACACGGATCGAACGGTGGTGTTCCGATGA

Upstream 100 bases:

>100_bases
ATTAAATTCCTGTAGAGAATTTTATTCGTCGACGAAAAACCTCTGCATCTGACCTTTTTCGCCCTGCGCCGAACTGCCGC
CACCCTTCAGAGGACACGCC

Downstream 100 bases:

>100_bases
GCGCACACATCATCGACGGCAAGGCCGCCGCCGCTCGCGTTCTGCAGCAGGTCAGACACGACGTGAACATCTTGAAGGCC
GAAGGCATCGAACCGGCGCT

Product: formyltetrahydrofolate deformylase

Products: NA

Alternate protein names: Formyl-FH(4) hydrolase [H]

Number of amino acids: Translated: 288; Mature: 288

Protein sequence:

>288_residues
MQHEKNHFILKISCPATSGIVAAVTSYLAGNSCYIGEMAQFDDEYSGTFFMRAVFRFNDGHEGDIQQLKAGFDAVASDFA
MQWELHDTRRPMRVLLMVSKFDHCLTDLLYRYHKGEMDMTITAIVSNHLDLRPMAEREGIRFIYLPVTRETKAAQEAALM
KVVDETGTELVVLARYMQILSDDLCQQLAGRAINIHHSFLPGFKGAKPYHQAYERGVKLIGATAHYVTSDLDEGPIIEQE
VQRVDHVYLPADLVAAGRNNETIALSRAVKYHLEHRVFLNTDRTVVFR

Sequences:

>Translated_288_residues
MQHEKNHFILKISCPATSGIVAAVTSYLAGNSCYIGEMAQFDDEYSGTFFMRAVFRFNDGHEGDIQQLKAGFDAVASDFA
MQWELHDTRRPMRVLLMVSKFDHCLTDLLYRYHKGEMDMTITAIVSNHLDLRPMAEREGIRFIYLPVTRETKAAQEAALM
KVVDETGTELVVLARYMQILSDDLCQQLAGRAINIHHSFLPGFKGAKPYHQAYERGVKLIGATAHYVTSDLDEGPIIEQE
VQRVDHVYLPADLVAAGRNNETIALSRAVKYHLEHRVFLNTDRTVVFR
>Mature_288_residues
MQHEKNHFILKISCPATSGIVAAVTSYLAGNSCYIGEMAQFDDEYSGTFFMRAVFRFNDGHEGDIQQLKAGFDAVASDFA
MQWELHDTRRPMRVLLMVSKFDHCLTDLLYRYHKGEMDMTITAIVSNHLDLRPMAEREGIRFIYLPVTRETKAAQEAALM
KVVDETGTELVVLARYMQILSDDLCQQLAGRAINIHHSFLPGFKGAKPYHQAYERGVKLIGATAHYVTSDLDEGPIIEQE
VQRVDHVYLPADLVAAGRNNETIALSRAVKYHLEHRVFLNTDRTVVFR

Specific function: Produces Formate From Formyl-Tetrahydrofolate. Provides The Major Source Of Formate For The Purt-Dependent Synthesis Of 5'-Phosphoribosyl-N-Formylglycinamide (Fgar) During Aerobic Growth. Has A Role In Regulating The One-Carbon Pool. [C]

COG id: COG0788

COG function: function code F; Formyltetrahydrofolate hydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ACT domain [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=194, Percent_Identity=31.4432989690722, Blast_Score=85, Evalue=7e-17,
Organism=Homo sapiens, GI209869995, Length=194, Percent_Identity=31.4432989690722, Blast_Score=85, Evalue=7e-17,
Organism=Homo sapiens, GI209869993, Length=194, Percent_Identity=31.4432989690722, Blast_Score=85, Evalue=7e-17,
Organism=Escherichia coli, GI1787483, Length=287, Percent_Identity=39.3728222996516, Blast_Score=201, Evalue=4e-53,
Organism=Escherichia coli, GI1788846, Length=151, Percent_Identity=31.1258278145695, Blast_Score=73, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17567511, Length=153, Percent_Identity=34.640522875817, Blast_Score=84, Evalue=6e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002376
- InterPro:   IPR004810 [H]

Pfam domain/function: PF00551 Formyl_trans_N [H]

EC number: =3.5.1.10 [H]

Molecular weight: Translated: 32671; Mature: 32671

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQHEKNHFILKISCPATSGIVAAVTSYLAGNSCYIGEMAQFDDEYSGTFFMRAVFRFNDG
CCCCCCEEEEEEECCCCCCHHHHHHHHHCCCCEEECCHHHCCCCCCCCEEEEEEECCCCC
HEGDIQQLKAGFDAVASDFAMQWELHDTRRPMRVLLMVSKFDHCLTDLLYRYHKGEMDMT
CCCHHHHHHHHHHHHHHHHHEEEEEHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEE
ITAIVSNHLDLRPMAEREGIRFIYLPVTRETKAAQEAALMKVVDETGTELVVLARYMQIL
EEEEHHCCCCCCCCHHHCCCEEEEEECCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
SDDLCQQLAGRAINIHHSFLPGFKGAKPYHQAYERGVKLIGATAHYVTSDLDEGPIIEQE
HHHHHHHHCCCEEEEEHHHCCCCCCCCHHHHHHHCCCEEEECHHHHHHCCCCCCCCCHHH
VQRVDHVYLPADLVAAGRNNETIALSRAVKYHLEHRVFLNTDRTVVFR
HHHHCEEECCHHHHCCCCCCCEEEHHHHHHHHHHCEEEEECCCEEEEC
>Mature Secondary Structure
MQHEKNHFILKISCPATSGIVAAVTSYLAGNSCYIGEMAQFDDEYSGTFFMRAVFRFNDG
CCCCCCEEEEEEECCCCCCHHHHHHHHHCCCCEEECCHHHCCCCCCCCEEEEEEECCCCC
HEGDIQQLKAGFDAVASDFAMQWELHDTRRPMRVLLMVSKFDHCLTDLLYRYHKGEMDMT
CCCHHHHHHHHHHHHHHHHHEEEEEHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEE
ITAIVSNHLDLRPMAEREGIRFIYLPVTRETKAAQEAALMKVVDETGTELVVLARYMQIL
EEEEHHCCCCCCCCHHHCCCEEEEEECCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
SDDLCQQLAGRAINIHHSFLPGFKGAKPYHQAYERGVKLIGATAHYVTSDLDEGPIIEQE
HHHHHHHHCCCEEEEEHHHCCCCCCCCHHHHHHHCCCEEEECHHHHHHCCCCCCCCCHHH
VQRVDHVYLPADLVAAGRNNETIALSRAVKYHLEHRVFLNTDRTVVFR
HHHHCEEECCHHHHCCCCCCCEEEHHHHHHHHHHCEEEEECCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7543100 [H]