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The map label for this gene is folD2

Identifier: 66045465

GI number: 66045465

Start: 2586853

End: 2587755

Strand: Reverse

Name: folD2

Synonym: Psyr_2225

Alternate gene names: 66045465

Gene position: 2587755-2586853 (Counterclockwise)

Preceding gene: 66045466

Following gene: 66045464

Centisome position: 42.47

GC content: 63.34

Gene sequence:

>903_bases
ATGAGCGCACACATCATCGACGGCAAGGCCGCCGCCGCTCGCGTTCTGCAGCAGGTCAGACACGACGTGAACATCTTGAA
GGCCGAAGGCATCGAACCGGCGCTGGCGGTCATTCTGGTCGGTAATGACGCGGCCAGTGAGGTCTACGTACGCAACAAGA
TCCTGCGCGCCGGGGAAGCCGGCATTCGCTCGCTGGAGCACCGCCTGCCGGCCGACAGCAGCCAGGCACGCTTACTGGCG
TTGATTGCCGAATTGAACGCCGACAACACGGTAAACGGCATTCTCCTGCAACTGCCCTTGCCCGCTCACATCGATGAAAA
CCGCGTCCTGCAGGCTATCGACCCGGACAAGGACGTTGACGGTTTTCACAGTGAAAACGTCGGTGGCCTGAGCCAGGGGC
GCAACGTACTGACGCCCTGCACCCCCAGCGGTTGCCTGCACTTGCTGGAAGAAACCTGCGGCGATCTGAGCGGCAAGCAT
GCGGTGGTGATCGGTCGCTCCAACATCGTCGGCAAACCCATGGCGGCTCTGCTGCTGCAAGCGCATTGCTCGGTGACGGT
GGTGCATTCGCGCAGTACCGACGCCAGGGCCTTGTGCCAGTTGGCCGACATCGTCGTGGCGGCGGTTGGCCGTCCGCGCC
TTATCGATGCCAGCTGGCTCAAGCGCGGCGCGGTGGTGATCGACGTCGGCATCAACCGCATCGAGGACCATGGCCGCAGC
CGCCTGGTGGGCGATGTCGATTTCGCCAGCGCCACCGAAGTCGCTTCTGCCATCACGCCCGTTCCCGGCGGAGTCGGCCC
GATGACTATCGCCTTTCTGATGAAAAACACCGTGACTGCTGCCCGGCAGCAGGCCCACGCGCAACGCAGCCAACCGGAGG
CCGTATGCCTTTCAACCTATTGA

Upstream 100 bases:

>100_bases
CGGCGGGACGCAATAACGAAACCATAGCGCTGTCGCGGGCCGTCAAATACCACCTGGAGCACCGCGTGTTTCTCAACACG
GATCGAACGGTGGTGTTCCG

Downstream 100 bases:

>100_bases
AATACGGGCTCAGCTCGCAGTACCCGGTCGAGGTGGATCTGCCTGCGCCCAAGGAACTGAAGTCCTCCTACGACGTGGTG
ATCATCGGCGGCGGCGGGCA

Product: bifunctional 5,10-methylene-tetrahydrofolate dehydrogenase/ 5,10-methylene-tetrahydrofolate cyclohydrolase

Products: NA

Alternate protein names: Methylenetetrahydrofolate dehydrogenase; Methenyltetrahydrofolate cyclohydrolase

Number of amino acids: Translated: 300; Mature: 299

Protein sequence:

>300_residues
MSAHIIDGKAAAARVLQQVRHDVNILKAEGIEPALAVILVGNDAASEVYVRNKILRAGEAGIRSLEHRLPADSSQARLLA
LIAELNADNTVNGILLQLPLPAHIDENRVLQAIDPDKDVDGFHSENVGGLSQGRNVLTPCTPSGCLHLLEETCGDLSGKH
AVVIGRSNIVGKPMAALLLQAHCSVTVVHSRSTDARALCQLADIVVAAVGRPRLIDASWLKRGAVVIDVGINRIEDHGRS
RLVGDVDFASATEVASAITPVPGGVGPMTIAFLMKNTVTAARQQAHAQRSQPEAVCLSTY

Sequences:

>Translated_300_residues
MSAHIIDGKAAAARVLQQVRHDVNILKAEGIEPALAVILVGNDAASEVYVRNKILRAGEAGIRSLEHRLPADSSQARLLA
LIAELNADNTVNGILLQLPLPAHIDENRVLQAIDPDKDVDGFHSENVGGLSQGRNVLTPCTPSGCLHLLEETCGDLSGKH
AVVIGRSNIVGKPMAALLLQAHCSVTVVHSRSTDARALCQLADIVVAAVGRPRLIDASWLKRGAVVIDVGINRIEDHGRS
RLVGDVDFASATEVASAITPVPGGVGPMTIAFLMKNTVTAARQQAHAQRSQPEAVCLSTY
>Mature_299_residues
SAHIIDGKAAAARVLQQVRHDVNILKAEGIEPALAVILVGNDAASEVYVRNKILRAGEAGIRSLEHRLPADSSQARLLAL
IAELNADNTVNGILLQLPLPAHIDENRVLQAIDPDKDVDGFHSENVGGLSQGRNVLTPCTPSGCLHLLEETCGDLSGKHA
VVIGRSNIVGKPMAALLLQAHCSVTVVHSRSTDARALCQLADIVVAAVGRPRLIDASWLKRGAVVIDVGINRIEDHGRSR
LVGDVDFASATEVASAITPVPGGVGPMTIAFLMKNTVTAARQQAHAQRSQPEAVCLSTY

Specific function: Catalyzes the oxidation of 5,10- methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10- formyltetrahydrofolate

COG id: COG0190

COG function: function code H; 5,10-methylene-tetrahydrofolate dehydrogenase/Methenyl tetrahydrofolate cyclohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family

Homologues:

Organism=Homo sapiens, GI222136639, Length=291, Percent_Identity=43.298969072165, Blast_Score=235, Evalue=4e-62,
Organism=Homo sapiens, GI94721354, Length=296, Percent_Identity=47.2972972972973, Blast_Score=217, Evalue=1e-56,
Organism=Homo sapiens, GI222418558, Length=295, Percent_Identity=44.4067796610169, Blast_Score=213, Evalue=2e-55,
Organism=Homo sapiens, GI36796743, Length=234, Percent_Identity=30.7692307692308, Blast_Score=85, Evalue=9e-17,
Organism=Escherichia coli, GI1786741, Length=289, Percent_Identity=50.8650519031142, Blast_Score=264, Evalue=6e-72,
Organism=Caenorhabditis elegans, GI17568735, Length=302, Percent_Identity=41.0596026490066, Blast_Score=224, Evalue=4e-59,
Organism=Saccharomyces cerevisiae, GI6319558, Length=288, Percent_Identity=44.0972222222222, Blast_Score=253, Evalue=3e-68,
Organism=Saccharomyces cerevisiae, GI6321643, Length=299, Percent_Identity=40.4682274247492, Blast_Score=223, Evalue=2e-59,
Organism=Saccharomyces cerevisiae, GI6322933, Length=295, Percent_Identity=25.7627118644068, Blast_Score=70, Evalue=6e-13,
Organism=Drosophila melanogaster, GI17136818, Length=302, Percent_Identity=48.3443708609272, Blast_Score=249, Evalue=2e-66,
Organism=Drosophila melanogaster, GI17136816, Length=302, Percent_Identity=48.3443708609272, Blast_Score=249, Evalue=2e-66,
Organism=Drosophila melanogaster, GI62472483, Length=298, Percent_Identity=43.9597315436242, Blast_Score=226, Evalue=1e-59,
Organism=Drosophila melanogaster, GI45551871, Length=298, Percent_Identity=43.9597315436242, Blast_Score=226, Evalue=1e-59,
Organism=Drosophila melanogaster, GI24645718, Length=298, Percent_Identity=43.9597315436242, Blast_Score=226, Evalue=1e-59,
Organism=Drosophila melanogaster, GI17137370, Length=298, Percent_Identity=43.9597315436242, Blast_Score=226, Evalue=1e-59,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): FOLD2_PSEU2 (Q4ZUA4)

Other databases:

- EMBL:   CP000075
- RefSeq:   YP_235306.1
- HSSP:   P11586
- ProteinModelPortal:   Q4ZUA4
- SMR:   Q4ZUA4
- STRING:   Q4ZUA4
- GeneID:   3367740
- GenomeReviews:   CP000075_GR
- KEGG:   psb:Psyr_2225
- NMPDR:   fig|205918.4.peg.2702
- eggNOG:   COG0190
- HOGENOM:   HBG328751
- OMA:   CLMMLRD
- ProtClustDB:   PRK14194
- BioCyc:   PSYR205918:PSYR_2225-MONOMER
- GO:   GO:0005488
- HAMAP:   MF_01576
- InterPro:   IPR016040
- InterPro:   IPR000672
- InterPro:   IPR020630
- InterPro:   IPR020867
- InterPro:   IPR020631
- Gene3D:   G3DSA:3.40.50.720
- PRINTS:   PR00085

Pfam domain/function: PF00763 THF_DHG_CYH; PF02882 THF_DHG_CYH_C

EC number: =1.5.1.5; =3.5.4.9

Molecular weight: Translated: 31674; Mature: 31543

Theoretical pI: Translated: 6.90; Mature: 6.90

Prosite motif: PS00766 THF_DHG_CYH_1; PS00767 THF_DHG_CYH_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAHIIDGKAAAARVLQQVRHDVNILKAEGIEPALAVILVGNDAASEVYVRNKILRAGEA
CCCEEECCHHHHHHHHHHHHHHHHEEECCCCCCEEEEEEECCCCHHHHHHHHHHHHHCHH
GIRSLEHRLPADSSQARLLALIAELNADNTVNGILLQLPLPAHIDENRVLQAIDPDKDVD
HHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCCEEEECCCCCCCC
GFHSENVGGLSQGRNVLTPCTPSGCLHLLEETCGDLSGKHAVVIGRSNIVGKPMAALLLQ
CCCCCCCCCHHCCCCEECCCCCHHHHHHHHHHHCCCCCCEEEEEECCCCCCCHHHHHHHH
AHCSVTVVHSRSTDARALCQLADIVVAAVGRPRLIDASWLKRGAVVIDVGINRIEDHGRS
HCCCEEEEECCCCCHHHHHHHHHHHHHHCCCCCEECHHHHHCCCEEEEECCHHHHHCCCC
RLVGDVDFASATEVASAITPVPGGVGPMTIAFLMKNTVTAARQQAHAQRSQPEAVCLSTY
CEECCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECC
>Mature Secondary Structure 
SAHIIDGKAAAARVLQQVRHDVNILKAEGIEPALAVILVGNDAASEVYVRNKILRAGEA
CCEEECCHHHHHHHHHHHHHHHHEEECCCCCCEEEEEEECCCCHHHHHHHHHHHHHCHH
GIRSLEHRLPADSSQARLLALIAELNADNTVNGILLQLPLPAHIDENRVLQAIDPDKDVD
HHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCCEEEECCCCCCCC
GFHSENVGGLSQGRNVLTPCTPSGCLHLLEETCGDLSGKHAVVIGRSNIVGKPMAALLLQ
CCCCCCCCCHHCCCCEECCCCCHHHHHHHHHHHCCCCCCEEEEEECCCCCCCHHHHHHHH
AHCSVTVVHSRSTDARALCQLADIVVAAVGRPRLIDASWLKRGAVVIDVGINRIEDHGRS
HCCCEEEEECCCCCHHHHHHHHHHHHHHCCCCCEECHHHHHCCCEEEEECCHHHHHCCCC
RLVGDVDFASATEVASAITPVPGGVGPMTIAFLMKNTVTAARQQAHAQRSQPEAVCLSTY
CEECCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA