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The map label for this gene is amiC [H]

Identifier: 66045445

GI number: 66045445

Start: 2561670

End: 2562851

Strand: Reverse

Name: amiC [H]

Synonym: Psyr_2205

Alternate gene names: 66045445

Gene position: 2562851-2561670 (Counterclockwise)

Preceding gene: 66045449

Following gene: 66045444

Centisome position: 42.06

GC content: 60.83

Gene sequence:

>1182_bases
ATGCACAACGACAAACGCCGTTTGATCAAGCATGCTCTGTTGCTGGGCGCGATCAGCCTGCTGCCGTTTGCCCTGATCGG
TCAGGCGCAGGCGGACGAGGCCGTGAAAGTCGGCATCATCCACTCGCTGACCGGCACCATGGCCATCAGTGAAGCCTCGG
TGGTCGACGCGGAAAAACTCGCCATCGAAGAGATCAACGCCAGTGGCGGCGTGATGGGCAAGACCATCGAGCCGATCGTC
GAAGACGGTGCCAGCGACTGGCCGACCTTCGCCGAGAAGGCGCGCAAGCTGCTGGAAAACGACCACGTCGCGGTCACCTT
TGGCGCGTTCACCTCGGCCTCGCGCAAAGCGGTGCTGCCGGTCTTCGAGCGCAACAAGGGCCTGCTCTACTACCCGACCT
TCTATGAAGGGCTGGAGAAATCCCCAGCCATTATCTACACCGGCGCCGAGGCCTCGCAGCAGACCCTGGCAGCCGTTACC
TGGCTGATGGCCAACAAGGGCAAGAGCGTCTATCTGGTCGGTTCCGACTACATCTGGCCACGCACCACCAACAAGCTGGC
GCGGGCGTCGGTGACCAAACAGGGCGGCTCCATCGTCGGCGAAGACTATCTGCCGCTGGGCGCCATCGAGTTTTCCTCGG
TGATCAACAAGATCAAGGCGGCCAAGCCGGACATCGTGCTGTCGACCATCGTCGGCGGCTCCAACGTAGCGTTCTACAAA
CAGCTCAAGGCCGCGGGTATCGACAGCAGTAACCAGACGCTGATGGGGCTGGCGATCACCGAAGAGGAAGTCACCGGCAT
CGGTGCGGAAAACCTCGTCGGCTTCCTGACCTGCATGAGCTATTTCCAGAGCCTGAAAAACCCGGTCAACGAGAAATTCG
TCCAGGCCTTCAAAACACGCTATGGGCAGCAGCGCGTGGTCGGTGACCCGATGGCAGCGGCGTATACCGCGGTATACCTG
TGGAAAAAGGCCGTCGAGAAAGCAGGCAGTTTCGAGGTGCCGAAAGTGATCGCCGCATCCTCGGAAATGACCCTCGATGC
GCCTGAAGGCGAGATCAAGGTGCACAAGGACAACCACCACCTGTGGAAGCGTGCGCGCATCGGCACCCTCAATGCGCAGG
GCCAGGTCGACGTGATCTACGAGTCGGCACCGATCGAGCCCAACCCCTTTCCCAAACTCTGA

Upstream 100 bases:

>100_bases
CGGACGCAGTTGTCCCGCCAGATTCGGCACAAAAGCCTGACCTCCCGCCCATTGATTTGGCCGGGACGTCGGGCTTTTTT
CATTCCTGGGAGATAAGTGA

Downstream 100 bases:

>100_bases
TTCCGGCCCGGGCGCGATCCGCTTCGCGCCCGCTTTCGTCAATGTGCAGCAACAGGTAGCGACGGCATGACTCTCGATAC
GCTGGTAATGCAAGTCTTCA

Product: extracellular ligand-binding receptor

Products: ADP; phosphate; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 393; Mature: 393

Protein sequence:

>393_residues
MHNDKRRLIKHALLLGAISLLPFALIGQAQADEAVKVGIIHSLTGTMAISEASVVDAEKLAIEEINASGGVMGKTIEPIV
EDGASDWPTFAEKARKLLENDHVAVTFGAFTSASRKAVLPVFERNKGLLYYPTFYEGLEKSPAIIYTGAEASQQTLAAVT
WLMANKGKSVYLVGSDYIWPRTTNKLARASVTKQGGSIVGEDYLPLGAIEFSSVINKIKAAKPDIVLSTIVGGSNVAFYK
QLKAAGIDSSNQTLMGLAITEEEVTGIGAENLVGFLTCMSYFQSLKNPVNEKFVQAFKTRYGQQRVVGDPMAAAYTAVYL
WKKAVEKAGSFEVPKVIAASSEMTLDAPEGEIKVHKDNHHLWKRARIGTLNAQGQVDVIYESAPIEPNPFPKL

Sequences:

>Translated_393_residues
MHNDKRRLIKHALLLGAISLLPFALIGQAQADEAVKVGIIHSLTGTMAISEASVVDAEKLAIEEINASGGVMGKTIEPIV
EDGASDWPTFAEKARKLLENDHVAVTFGAFTSASRKAVLPVFERNKGLLYYPTFYEGLEKSPAIIYTGAEASQQTLAAVT
WLMANKGKSVYLVGSDYIWPRTTNKLARASVTKQGGSIVGEDYLPLGAIEFSSVINKIKAAKPDIVLSTIVGGSNVAFYK
QLKAAGIDSSNQTLMGLAITEEEVTGIGAENLVGFLTCMSYFQSLKNPVNEKFVQAFKTRYGQQRVVGDPMAAAYTAVYL
WKKAVEKAGSFEVPKVIAASSEMTLDAPEGEIKVHKDNHHLWKRARIGTLNAQGQVDVIYESAPIEPNPFPKL
>Mature_393_residues
MHNDKRRLIKHALLLGAISLLPFALIGQAQADEAVKVGIIHSLTGTMAISEASVVDAEKLAIEEINASGGVMGKTIEPIV
EDGASDWPTFAEKARKLLENDHVAVTFGAFTSASRKAVLPVFERNKGLLYYPTFYEGLEKSPAIIYTGAEASQQTLAAVT
WLMANKGKSVYLVGSDYIWPRTTNKLARASVTKQGGSIVGEDYLPLGAIEFSSVINKIKAAKPDIVLSTIVGGSNVAFYK
QLKAAGIDSSNQTLMGLAITEEEVTGIGAENLVGFLTCMSYFQSLKNPVNEKFVQAFKTRYGQQRVVGDPMAAAYTAVYL
WKKAVEKAGSFEVPKVIAASSEMTLDAPEGEIKVHKDNHHLWKRARIGTLNAQGQVDVIYESAPIEPNPFPKL

Specific function: Negatively regulates the expression of the aliphatic amidase operon. AmiC functions by inhibiting the action of AmiR at the protein level. It exhibits protein kinase activity [H]

COG id: COG0683

COG function: function code E; ABC-type branched-chain amino acid transport systems, periplasmic component

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000709 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 42492; Mature: 42492

Theoretical pI: Translated: 7.76; Mature: 7.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHNDKRRLIKHALLLGAISLLPFALIGQAQADEAVKVGIIHSLTGTMAISEASVVDAEKL
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEHHHHHHHEECCHHHHHHHHH
AIEEINASGGVMGKTIEPIVEDGASDWPTFAEKARKLLENDHVAVTFGAFTSASRKAVLP
HHHHCCCCCCCCCCCHHHHHHCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCCEECC
VFERNKGLLYYPTFYEGLEKSPAIIYTGAEASQQTLAAVTWLMANKGKSVYLVGSDYIWP
CEECCCCEEECCHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCEEEEEECCEECC
RTTNKLARASVTKQGGSIVGEDYLPLGAIEFSSVINKIKAAKPDIVLSTIVGGSNVAFYK
CCCHHHHHHHHHHCCCCEECCCCCCCCHHHHHHHHHHHHCCCCCEEEEEEECCCCHHHHH
QLKAAGIDSSNQTLMGLAITEEEVTGIGAENLVGFLTCMSYFQSLKNPVNEKFVQAFKTR
HHHHHCCCCCCCEEEEEEEEHHHHHCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHH
YGQQRVVGDPMAAAYTAVYLWKKAVEKAGSFEVPKVIAASSEMTLDAPEGEIKVHKDNHH
CCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHEECCCCEEEECCCCCEEEEECCHH
LWKRARIGTLNAQGQVDVIYESAPIEPNPFPKL
HHHHHCCCCCCCCCCEEEEEECCCCCCCCCCCC
>Mature Secondary Structure
MHNDKRRLIKHALLLGAISLLPFALIGQAQADEAVKVGIIHSLTGTMAISEASVVDAEKL
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEHHHHHHHEECCHHHHHHHHH
AIEEINASGGVMGKTIEPIVEDGASDWPTFAEKARKLLENDHVAVTFGAFTSASRKAVLP
HHHHCCCCCCCCCCCHHHHHHCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCCEECC
VFERNKGLLYYPTFYEGLEKSPAIIYTGAEASQQTLAAVTWLMANKGKSVYLVGSDYIWP
CEECCCCEEECCHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCEEEEEECCEECC
RTTNKLARASVTKQGGSIVGEDYLPLGAIEFSSVINKIKAAKPDIVLSTIVGGSNVAFYK
CCCHHHHHHHHHHCCCCEECCCCCCCCHHHHHHHHHHHHCCCCCEEEEEEECCCCHHHHH
QLKAAGIDSSNQTLMGLAITEEEVTGIGAENLVGFLTCMSYFQSLKNPVNEKFVQAFKTR
HHHHHCCCCCCCEEEEEEEEHHHHHCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHH
YGQQRVVGDPMAAAYTAVYLWKKAVEKAGSFEVPKVIAASSEMTLDAPEGEIKVHKDNHH
CCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHEECCCCEEEECCCCCEEEEECCHH
LWKRARIGTLNAQGQVDVIYESAPIEPNPFPKL
HHHHHCCCCCCCCCCEEEEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1907262; 10984043; 1762155; 7813419; 10508151; 10708652 [H]