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The map label for this gene is gltX [H]

Identifier: 66045217

GI number: 66045217

Start: 2294713

End: 2296194

Strand: Direct

Name: gltX [H]

Synonym: Psyr_1977

Alternate gene names: 66045217

Gene position: 2294713-2296194 (Clockwise)

Preceding gene: 66045214

Following gene: 66045218

Centisome position: 37.66

GC content: 60.8

Gene sequence:

>1482_bases
ATGACCACCGTTCGTACCCGCATCGCGCCATCGCCCACAGGCGACCCCCACGTCGGCACGGCCTACATCGCGCTGTTCAA
CTACTGCTTTGCCAAACAGCACGGCGGCGAGTTCATCCTGCGCATTGAAGACACCGATCAACTGCGCTCTACCCGCGAGT
CCGAACAGCAGATCTTCGACGCACTGCGCTGGCTGGGCATCGACTGGAGCGAAGGCCCCGACGTCGGTGGTCCTCACGGT
CCCTACCGCCAGAGCGAGCGCGGCGACATCTACCAGAAATACGCGCAGCAACTGGTCGACATGGGCCATGCCTTCCCGTG
CTTCTGCACCGCCGAAGAACTCGACCAGATGCGCGCCGAACAGCAGGCCAAGGGCGAAACCCCGCGCTATGACGGTCGTG
CCTTGCTGCTCTCCAAGGAAGAGGTTCAGCGCCGCCTGGATGCCGGCGAGCCACACGTGATCCGCATGAAAGTGCCGACC
GAAGGTGTCTGCGTAGTACCTGACATGCTGCGTGGCGAAGTCGAAATCCCGTGGGACCGCATGGACATGCAGGTCCTGAT
GAAGACCGACGGCCTGCCGACCTACTTCCTCGCCAACGTGGTCGATGACCACCTGATGGGCATCACCCATGTACTGCGCG
GCGAAGAATGGCTGCCTTCGGCGCCGAAACTGATCCTGCTGTACGAATACTTCGGCTGGGACAAGCCGCAGCTGTGCTAC
ATGCCGCTGCTGCGTAACCCCGACAAGAGCAAGCTGTCCAAGCGCAAGAACCCCACCTCGGTGACCTTCTACGAGCGCAT
GGGCTTCATGCCCGAGGCGATGCTCAACTACCTGGGGCGCATGGGCTGGTCGATGCCTGACGAGCGCGAGAAGTTCTCCC
TGCAGGAGATGGTCGACAACTTCGACCTGTCGCGTGTTTCGTTGGGCGGGCCGATCTTCGATATCGAGAAGCTTTCCTGG
CTCAATGGCCAATGGCTGCGTGATCTGCCGGTCGAGGAGTTCGCCTCGCGTCTGAAGACCTGGGCGCTGAATCCTGACTA
CATGATGAAGATTGCACCGCATGTGCAGGGCAGGGTAGAGACCTTCAGTCAGGTCGCACCGCTGGCCGGCTTCTTCTTCG
CAGGTGGCGTCACGCCTGATGTGAAGCTGTTCGAGCACAAGAAGCTGTCCCCGGAGCAGGTGCGTCAGGTGATGCAACTG
ATCCTGTGGAAGCTGGAATCGCTGCGTCAGTGGGAGAAGGAGCGGATCATGGGCTGCATCCAGGCCGTGGTCGAGCATCT
TGAACTCAAGCTGCGTGATGCCATGCCACTGATGTTCGCTGCGATCACCGGCCAGGCCAATTCGGTGTCGGTGACCGATG
CGATGGAAATCCTCGGTCCGGACCTGACCCGCTTCCGGCTGCGTCAGGCCCTCGATTTGCTCGGTGGCGTTTCCAAGAAA
GAAAACAAGGAATGGGAAAAGCTTCTGGGCTCTATCGCCTGA

Upstream 100 bases:

>100_bases
TGAGCGCGGAGCGCGAACGCAATCACATTGCCCGTGCTGGAGCCCCATGCCCCCGGCCTGTTAACATTCCGCGCTTGATC
GACCTCATTCGGGATTTTCC

Downstream 100 bases:

>100_bases
GGCGTTACCTGTTCCTGTGAGTCGCCCCCGGTTTTCCGGGGGTTGAATGGTAAGTGGTTGTATTACCGACAAAAAAGTTT
GCAGATTGTAGAAAATAAAT

Product: glutamyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase; GluRS [H]

Number of amino acids: Translated: 493; Mature: 492

Protein sequence:

>493_residues
MTTVRTRIAPSPTGDPHVGTAYIALFNYCFAKQHGGEFILRIEDTDQLRSTRESEQQIFDALRWLGIDWSEGPDVGGPHG
PYRQSERGDIYQKYAQQLVDMGHAFPCFCTAEELDQMRAEQQAKGETPRYDGRALLLSKEEVQRRLDAGEPHVIRMKVPT
EGVCVVPDMLRGEVEIPWDRMDMQVLMKTDGLPTYFLANVVDDHLMGITHVLRGEEWLPSAPKLILLYEYFGWDKPQLCY
MPLLRNPDKSKLSKRKNPTSVTFYERMGFMPEAMLNYLGRMGWSMPDEREKFSLQEMVDNFDLSRVSLGGPIFDIEKLSW
LNGQWLRDLPVEEFASRLKTWALNPDYMMKIAPHVQGRVETFSQVAPLAGFFFAGGVTPDVKLFEHKKLSPEQVRQVMQL
ILWKLESLRQWEKERIMGCIQAVVEHLELKLRDAMPLMFAAITGQANSVSVTDAMEILGPDLTRFRLRQALDLLGGVSKK
ENKEWEKLLGSIA

Sequences:

>Translated_493_residues
MTTVRTRIAPSPTGDPHVGTAYIALFNYCFAKQHGGEFILRIEDTDQLRSTRESEQQIFDALRWLGIDWSEGPDVGGPHG
PYRQSERGDIYQKYAQQLVDMGHAFPCFCTAEELDQMRAEQQAKGETPRYDGRALLLSKEEVQRRLDAGEPHVIRMKVPT
EGVCVVPDMLRGEVEIPWDRMDMQVLMKTDGLPTYFLANVVDDHLMGITHVLRGEEWLPSAPKLILLYEYFGWDKPQLCY
MPLLRNPDKSKLSKRKNPTSVTFYERMGFMPEAMLNYLGRMGWSMPDEREKFSLQEMVDNFDLSRVSLGGPIFDIEKLSW
LNGQWLRDLPVEEFASRLKTWALNPDYMMKIAPHVQGRVETFSQVAPLAGFFFAGGVTPDVKLFEHKKLSPEQVRQVMQL
ILWKLESLRQWEKERIMGCIQAVVEHLELKLRDAMPLMFAAITGQANSVSVTDAMEILGPDLTRFRLRQALDLLGGVSKK
ENKEWEKLLGSIA
>Mature_492_residues
TTVRTRIAPSPTGDPHVGTAYIALFNYCFAKQHGGEFILRIEDTDQLRSTRESEQQIFDALRWLGIDWSEGPDVGGPHGP
YRQSERGDIYQKYAQQLVDMGHAFPCFCTAEELDQMRAEQQAKGETPRYDGRALLLSKEEVQRRLDAGEPHVIRMKVPTE
GVCVVPDMLRGEVEIPWDRMDMQVLMKTDGLPTYFLANVVDDHLMGITHVLRGEEWLPSAPKLILLYEYFGWDKPQLCYM
PLLRNPDKSKLSKRKNPTSVTFYERMGFMPEAMLNYLGRMGWSMPDEREKFSLQEMVDNFDLSRVSLGGPIFDIEKLSWL
NGQWLRDLPVEEFASRLKTWALNPDYMMKIAPHVQGRVETFSQVAPLAGFFFAGGVTPDVKLFEHKKLSPEQVRQVMQLI
LWKLESLRQWEKERIMGCIQAVVEHLELKLRDAMPLMFAAITGQANSVSVTDAMEILGPDLTRFRLRQALDLLGGVSKKE
NKEWEKLLGSIA

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) [H]

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI134288884, Length=498, Percent_Identity=34.7389558232932, Blast_Score=276, Evalue=5e-74,
Organism=Escherichia coli, GI1788743, Length=490, Percent_Identity=35.9183673469388, Blast_Score=297, Evalue=1e-81,
Organism=Escherichia coli, GI226510925, Length=306, Percent_Identity=30.718954248366, Blast_Score=108, Evalue=5e-25,
Organism=Caenorhabditis elegans, GI71993394, Length=486, Percent_Identity=32.7160493827161, Blast_Score=261, Evalue=7e-70,
Organism=Saccharomyces cerevisiae, GI6324540, Length=501, Percent_Identity=33.7325349301397, Blast_Score=259, Evalue=6e-70,
Organism=Saccharomyces cerevisiae, GI6324742, Length=312, Percent_Identity=24.6794871794872, Blast_Score=68, Evalue=4e-12,
Organism=Drosophila melanogaster, GI24665321, Length=491, Percent_Identity=34.4195519348269, Blast_Score=267, Evalue=1e-71,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008925
- InterPro:   IPR020751
- InterPro:   IPR001412
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00749 tRNA-synt_1c [H]

EC number: =6.1.1.17 [H]

Molecular weight: Translated: 56507; Mature: 56376

Theoretical pI: Translated: 5.71; Mature: 5.71

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTVRTRIAPSPTGDPHVGTAYIALFNYCFAKQHGGEFILRIEDTDQLRSTRESEQQIFD
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHHHH
ALRWLGIDWSEGPDVGGPHGPYRQSERGDIYQKYAQQLVDMGHAFPCFCTAEELDQMRAE
HHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCEECHHHHHHHHHH
QQAKGETPRYDGRALLLSKEEVQRRLDAGEPHVIRMKVPTEGVCVVPDMLRGEVEIPWDR
HHHCCCCCCCCCCEEEECHHHHHHHHCCCCCEEEEEECCCCCEEECCHHHCCCEECCHHH
MDMQVLMKTDGLPTYFLANVVDDHLMGITHVLRGEEWLPSAPKLILLYEYFGWDKPQLCY
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEHHHCCCCCCEEE
MPLLRNPDKSKLSKRKNPTSVTFYERMGFMPEAMLNYLGRMGWSMPDEREKFSLQEMVDN
HHHHCCCCHHHHHHCCCCCEEEHHHHHCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHCC
FDLSRVSLGGPIFDIEKLSWLNGQWLRDLPVEEFASRLKTWALNPDYMMKIAPHVQGRVE
CCCCEEECCCCEEEHHHHHHCCCCHHHCCCHHHHHHHHHHHCCCCHHHHEECHHHHHHHH
TFSQVAPLAGFFFAGGVTPDVKLFEHKKLSPEQVRQVMQLILWKLESLRQWEKERIMGCI
HHHHHHHHHHHHHHCCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QAVVEHLELKLRDAMPLMFAAITGQANSVSVTDAMEILGPDLTRFRLRQALDLLGGVSKK
HHHHHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHCCCHHHHHHHHHHHHHCCCCCC
ENKEWEKLLGSIA
CCHHHHHHHHCCC
>Mature Secondary Structure 
TTVRTRIAPSPTGDPHVGTAYIALFNYCFAKQHGGEFILRIEDTDQLRSTRESEQQIFD
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHHHH
ALRWLGIDWSEGPDVGGPHGPYRQSERGDIYQKYAQQLVDMGHAFPCFCTAEELDQMRAE
HHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCEECHHHHHHHHHH
QQAKGETPRYDGRALLLSKEEVQRRLDAGEPHVIRMKVPTEGVCVVPDMLRGEVEIPWDR
HHHCCCCCCCCCCEEEECHHHHHHHHCCCCCEEEEEECCCCCEEECCHHHCCCEECCHHH
MDMQVLMKTDGLPTYFLANVVDDHLMGITHVLRGEEWLPSAPKLILLYEYFGWDKPQLCY
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEHHHCCCCCCEEE
MPLLRNPDKSKLSKRKNPTSVTFYERMGFMPEAMLNYLGRMGWSMPDEREKFSLQEMVDN
HHHHCCCCHHHHHHCCCCCEEEHHHHHCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHCC
FDLSRVSLGGPIFDIEKLSWLNGQWLRDLPVEEFASRLKTWALNPDYMMKIAPHVQGRVE
CCCCEEECCCCEEEHHHHHHCCCCHHHCCCHHHHHHHHHHHCCCCHHHHEECHHHHHHHH
TFSQVAPLAGFFFAGGVTPDVKLFEHKKLSPEQVRQVMQLILWKLESLRQWEKERIMGCI
HHHHHHHHHHHHHHCCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QAVVEHLELKLRDAMPLMFAAITGQANSVSVTDAMEILGPDLTRFRLRQALDLLGGVSKK
HHHHHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHCCCHHHHHHHHHHHHHCCCCCC
ENKEWEKLLGSIA
CCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA