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The map label for this gene is rutR [H]

Identifier: 66045022

GI number: 66045022

Start: 2024775

End: 2025383

Strand: Reverse

Name: rutR [H]

Synonym: Psyr_1777

Alternate gene names: 66045022

Gene position: 2025383-2024775 (Counterclockwise)

Preceding gene: 66045023

Following gene: 66045021

Centisome position: 33.24

GC content: 59.93

Gene sequence:

>609_bases
ATGAGCAGTATCCGCGAGCGCAACAAAGAACTCATCCTGCGCGCTGCCAGCGAGGAATTCGCCGACAAAGGCTTTGCCGC
CAGCAAGACCAGCGACATCGCGGCCAAGGCCGGTGTCCCCAAGCCCAACGTCTATTACTACTTCAAATCCAAGGAAAACC
TCTATCGCGAGGTGCTGGAAAGCATCATCGAGCCCATTCTGCGTGCGTCGACGCCCTTCAACCCCGAAGGCGTGCCTGCC
GAAGTCCTCAGCCGCTATATCCGTTCGAAAATCCAGATTTCCCGTGACCTGCCCTTCGCCTCGAAAGTGTTCGCCAGCGA
GATCATGCATGGCGCCCCGCACCTGACAGCGCAGCAGATCGAACAGCTCAATGGCCAGGCACGGCACAACATCGAATGCA
TCCAGGCCTGGATCGACAGCGGACAGATCGCCCCGCTCGATCCTCATCACCTGATGTTCACCATCTGGGCCGCGACCCAG
ACCTACGCCGATTTCGACTGGCAGATCGCAACGGTCACCGGCAAGGCAAAACTGCAGGATGCCGATTACGAGGCGGCCAC
CCGGACCATCCTCAGGCTGGTGCTCAAAGGCTGCGAGCCGGACCGCTGA

Upstream 100 bases:

>100_bases
CGGCCCGGTGCAACCCTGCCCCGGGCCGTCAACATTGAGCGCTGCGCAAACCCGGTGCTAACATGCGCGACCGCCATGCA
GGCAAGGGAATAACCGGGTC

Downstream 100 bases:

>100_bases
ACCCACCCTGCTTTCCTGCAGACGCAAAAAACCCGCCGAAGCGGGTGGTTTTGCAACATGACCATTCCGACATCCTGTCA
GTAGCCTCCTGGCAATGGTC

Product: regulatory protein, TetR

Products: NA

Alternate protein names: Rut operon repressor [H]

Number of amino acids: Translated: 202; Mature: 201

Protein sequence:

>202_residues
MSSIRERNKELILRAASEEFADKGFAASKTSDIAAKAGVPKPNVYYYFKSKENLYREVLESIIEPILRASTPFNPEGVPA
EVLSRYIRSKIQISRDLPFASKVFASEIMHGAPHLTAQQIEQLNGQARHNIECIQAWIDSGQIAPLDPHHLMFTIWAATQ
TYADFDWQIATVTGKAKLQDADYEAATRTILRLVLKGCEPDR

Sequences:

>Translated_202_residues
MSSIRERNKELILRAASEEFADKGFAASKTSDIAAKAGVPKPNVYYYFKSKENLYREVLESIIEPILRASTPFNPEGVPA
EVLSRYIRSKIQISRDLPFASKVFASEIMHGAPHLTAQQIEQLNGQARHNIECIQAWIDSGQIAPLDPHHLMFTIWAATQ
TYADFDWQIATVTGKAKLQDADYEAATRTILRLVLKGCEPDR
>Mature_201_residues
SSIRERNKELILRAASEEFADKGFAASKTSDIAAKAGVPKPNVYYYFKSKENLYREVLESIIEPILRASTPFNPEGVPAE
VLSRYIRSKIQISRDLPFASKVFASEIMHGAPHLTAQQIEQLNGQARHNIECIQAWIDSGQIAPLDPHHLMFTIWAATQT
YADFDWQIATVTGKAKLQDADYEAATRTILRLVLKGCEPDR

Specific function: Master transcription regulator which represses the degradation of pyrimidines (rutABCDEFG) and purines (gcl operon) for maintenance of metabolic balance between pyrimidines and purines. It also regulates the synthesis of pyrimidine nucleotides and arginin

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH tetR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787249, Length=193, Percent_Identity=37.8238341968912, Blast_Score=142, Evalue=1e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR015893
- InterPro:   IPR011075
- InterPro:   IPR019915
- InterPro:   IPR001647
- InterPro:   IPR013573 [H]

Pfam domain/function: PF08362 TetR_C_3; PF00440 TetR_N [H]

EC number: NA

Molecular weight: Translated: 22707; Mature: 22576

Theoretical pI: Translated: 7.06; Mature: 7.06

Prosite motif: PS50977 HTH_TETR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSIRERNKELILRAASEEFADKGFAASKTSDIAAKAGVPKPNVYYYFKSKENLYREVLE
CCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCCCCCEEEEECCHHHHHHHHHH
SIIEPILRASTPFNPEGVPAEVLSRYIRSKIQISRDLPFASKVFASEIMHGAPHLTAQQI
HHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCHHHH
EQLNGQARHNIECIQAWIDSGQIAPLDPHHLMFTIWAATQTYADFDWQIATVTGKAKLQD
HHHCCCCHHHHHHHHHHHCCCCCCCCCCCCEEEEEEHHHHHHCCCCEEEEEEECCCCCCC
ADYEAATRTILRLVLKGCEPDR
CCHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
SSIRERNKELILRAASEEFADKGFAASKTSDIAAKAGVPKPNVYYYFKSKENLYREVLE
CCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCCCCCEEEEECCHHHHHHHHHH
SIIEPILRASTPFNPEGVPAEVLSRYIRSKIQISRDLPFASKVFASEIMHGAPHLTAQQI
HHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCHHHH
EQLNGQARHNIECIQAWIDSGQIAPLDPHHLMFTIWAATQTYADFDWQIATVTGKAKLQD
HHHCCCCHHHHHHHHHHHCCCCCCCCCCCCEEEEEEHHHHHHCCCCEEEEEEECCCCCCC
ADYEAATRTILRLVLKGCEPDR
CCHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]