BacMap

Definition	Pseudomonas syringae pv. syringae B728a, complete genome.
Accession	NC_007005
Length	6,093,698

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The map label for this gene is yeaE [H]

Identifier: 66045020

GI number: 66045020

Start: 2021862

End: 2022683

Strand: Reverse

Name: yeaE [H]

Synonym: Psyr_1775

Alternate gene names: 66045020

Gene position: 2022683-2021862 (Counterclockwise)

Preceding gene: 66045021

Following gene: 66045019

Centisome position: 33.19

GC content: 61.8

Gene sequence:

>822_bases
ATGCGCACACTGGAACTGGCAGGCAGAACCGTACCAGTGCTGGGTCAGGGCACCTGGCACATGGGTGAAAAAGGGCATCA
GCGCTCGGCAGAAATCGCCGGCCTGTGCCTCGGTATCGAACAGGGCCTGACCCTGATCGACACCGCCGAGATGTACGCCG
ATGGCGCTGCCGAGGAAATCGTCGGGCAAGCCATTGCCGGACAGCGGGACAAGGTATTCGTGGTCAGCAAGGTCTACCCG
CACAACGCCAGCCGCGCCGGTATTCCAACCGCGTGCGAGGCCAGCCTGCGGCGCATGAACACCGACTACATCGATCTGTA
CTTGCTGCACTGGCCAGGCCAGTACCCGCTGACCGAGACGGTCGAGGCATTCGAGCGTCTGCGTGAAGCGGGCAAAATCG
GTGCCTGGGGGGTGTCCAACTTTGATGTGCCGGACATGATCGAGCTCAACAACCCGCGTTGTGCCACCAATCAGGTGCTC
TACAACCCGGAGCAGCGCGGCATCGAGTTCGACCTGATGCTGTGGAGCGCAGACCGTCATCTGCCCTTGATGGCTTACTG
CCCGATCGGCCAGGCTGGCGATCTGCTCTACCACCCTGCCCTGCACGAAATCGCCAAACGCCACGATGCAACACCGGCGC
AGATTTCCCTGGCGTGGGTGCTTCGCCAGGACAACATGATCGCCATTCCCAAGGCGACCAACCCGGAACACATTCGTCTG
AACATCGCTGCCGAGCAGATTCGTCTGACCGAGCGCGATCTGGCCGATATCGATCTGGCATGGCCTGCGCCGACTCGCAA
GGTGCCGTTGGCGATGGTCTGA

Upstream 100 bases:

>100_bases
CATTGTCATTGGGGTGTCACGGAACCTCGTGCGCCTCTTGTTGCCTGAGACCTGCTTATTCAACCGCTCATTAGCTGCTT
ATTTAATTGCAGGGGGTCAC

Downstream 100 bases:

>100_bases
CCGCTGTCGAGGCGGTAGCGAGCTGTCGATTGACTGAACGGCTGCCCATGCAGTCGTTTCTCGATCTGGCGGATGTCACA
CCCGGCAACAAGCTGTCACA

Product: aldo/keto reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 273; Mature: 273

Protein sequence:

>273_residues
MRTLELAGRTVPVLGQGTWHMGEKGHQRSAEIAGLCLGIEQGLTLIDTAEMYADGAAEEIVGQAIAGQRDKVFVVSKVYP
HNASRAGIPTACEASLRRMNTDYIDLYLLHWPGQYPLTETVEAFERLREAGKIGAWGVSNFDVPDMIELNNPRCATNQVL
YNPEQRGIEFDLMLWSADRHLPLMAYCPIGQAGDLLYHPALHEIAKRHDATPAQISLAWVLRQDNMIAIPKATNPEHIRL
NIAAEQIRLTERDLADIDLAWPAPTRKVPLAMV

Sequences:

>Translated_273_residues
MRTLELAGRTVPVLGQGTWHMGEKGHQRSAEIAGLCLGIEQGLTLIDTAEMYADGAAEEIVGQAIAGQRDKVFVVSKVYP
HNASRAGIPTACEASLRRMNTDYIDLYLLHWPGQYPLTETVEAFERLREAGKIGAWGVSNFDVPDMIELNNPRCATNQVL
YNPEQRGIEFDLMLWSADRHLPLMAYCPIGQAGDLLYHPALHEIAKRHDATPAQISLAWVLRQDNMIAIPKATNPEHIRL
NIAAEQIRLTERDLADIDLAWPAPTRKVPLAMV
>Mature_273_residues
MRTLELAGRTVPVLGQGTWHMGEKGHQRSAEIAGLCLGIEQGLTLIDTAEMYADGAAEEIVGQAIAGQRDKVFVVSKVYP
HNASRAGIPTACEASLRRMNTDYIDLYLLHWPGQYPLTETVEAFERLREAGKIGAWGVSNFDVPDMIELNNPRCATNQVL
YNPEQRGIEFDLMLWSADRHLPLMAYCPIGQAGDLLYHPALHEIAKRHDATPAQISLAWVLRQDNMIAIPKATNPEHIRL
NIAAEQIRLTERDLADIDLAWPAPTRKVPLAMV

Specific function: Unknown

COG id: COG0656

COG function: function code R; Aldo/keto reductases, related to diketogulonate reductase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI310109922, Length=276, Percent_Identity=31.8840579710145, Blast_Score=108, Evalue=4e-24,
Organism=Homo sapiens, GI24497585, Length=293, Percent_Identity=30.3754266211604, Blast_Score=105, Evalue=3e-23,
Organism=Homo sapiens, GI310109920, Length=296, Percent_Identity=30.4054054054054, Blast_Score=102, Evalue=3e-22,
Organism=Homo sapiens, GI45446745, Length=296, Percent_Identity=30.4054054054054, Blast_Score=102, Evalue=4e-22,
Organism=Homo sapiens, GI4503285, Length=296, Percent_Identity=30.4054054054054, Blast_Score=102, Evalue=4e-22,
Organism=Homo sapiens, GI223468663, Length=296, Percent_Identity=28.7162162162162, Blast_Score=102, Evalue=5e-22,
Organism=Homo sapiens, GI5453543, Length=295, Percent_Identity=29.8305084745763, Blast_Score=101, Evalue=9e-22,
Organism=Homo sapiens, GI24497583, Length=293, Percent_Identity=30.0341296928328, Blast_Score=100, Evalue=1e-21,
Organism=Homo sapiens, GI93277124, Length=298, Percent_Identity=26.8456375838926, Blast_Score=93, Evalue=2e-19,
Organism=Homo sapiens, GI24497577, Length=288, Percent_Identity=27.7777777777778, Blast_Score=93, Evalue=2e-19,
Organism=Homo sapiens, GI5174391, Length=288, Percent_Identity=27.7777777777778, Blast_Score=93, Evalue=2e-19,
Organism=Homo sapiens, GI291291012, Length=253, Percent_Identity=29.6442687747036, Blast_Score=92, Evalue=5e-19,
Organism=Homo sapiens, GI5174695, Length=293, Percent_Identity=27.3037542662116, Blast_Score=88, Evalue=9e-18,
Organism=Homo sapiens, GI4502049, Length=298, Percent_Identity=28.5234899328859, Blast_Score=79, Evalue=4e-15,
Organism=Homo sapiens, GI300116273, Length=277, Percent_Identity=27.0758122743682, Blast_Score=79, Evalue=4e-15,
Organism=Homo sapiens, GI300116271, Length=268, Percent_Identity=25.3731343283582, Blast_Score=72, Evalue=6e-13,
Organism=Homo sapiens, GI27436969, Length=322, Percent_Identity=26.3975155279503, Blast_Score=72, Evalue=6e-13,
Organism=Homo sapiens, GI4504825, Length=322, Percent_Identity=26.3975155279503, Blast_Score=72, Evalue=7e-13,
Organism=Homo sapiens, GI27436964, Length=322, Percent_Identity=26.3975155279503, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI27436966, Length=322, Percent_Identity=26.3975155279503, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI27436962, Length=322, Percent_Identity=25.776397515528, Blast_Score=71, Evalue=1e-12,
Organism=Escherichia coli, GI1788081, Length=272, Percent_Identity=52.5735294117647, Blast_Score=285, Evalue=2e-78,
Organism=Escherichia coli, GI1786400, Length=253, Percent_Identity=29.2490118577075, Blast_Score=108, Evalue=3e-25,
Organism=Escherichia coli, GI87082198, Length=265, Percent_Identity=30.5660377358491, Blast_Score=99, Evalue=4e-22,
Organism=Escherichia coli, GI1789199, Length=336, Percent_Identity=30.6547619047619, Blast_Score=98, Evalue=5e-22,
Organism=Escherichia coli, GI1788070, Length=302, Percent_Identity=28.8079470198675, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI87081735, Length=273, Percent_Identity=26.7399267399267, Blast_Score=77, Evalue=9e-16,
Organism=Escherichia coli, GI1787674, Length=283, Percent_Identity=24.7349823321555, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI48994888, Length=245, Percent_Identity=27.3469387755102, Blast_Score=64, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17552492, Length=274, Percent_Identity=27.7372262773723, Blast_Score=102, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI17561298, Length=272, Percent_Identity=27.9411764705882, Blast_Score=99, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17564128, Length=298, Percent_Identity=28.1879194630872, Blast_Score=95, Evalue=5e-20,
Organism=Caenorhabditis elegans, GI71998625, Length=270, Percent_Identity=27.4074074074074, Blast_Score=92, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI17537077, Length=303, Percent_Identity=27.3927392739274, Blast_Score=87, Evalue=6e-18,
Organism=Caenorhabditis elegans, GI17537075, Length=303, Percent_Identity=27.0627062706271, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17566692, Length=281, Percent_Identity=27.7580071174377, Blast_Score=83, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17550248, Length=292, Percent_Identity=24.6575342465753, Blast_Score=82, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI17561300, Length=272, Percent_Identity=26.8382352941176, Blast_Score=81, Evalue=7e-16,
Organism=Caenorhabditis elegans, GI17538386, Length=276, Percent_Identity=24.6376811594203, Blast_Score=73, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6320576, Length=293, Percent_Identity=30.3754266211604, Blast_Score=102, Evalue=8e-23,
Organism=Saccharomyces cerevisiae, GI6324694, Length=272, Percent_Identity=27.9411764705882, Blast_Score=101, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6320079, Length=281, Percent_Identity=23.8434163701068, Blast_Score=72, Evalue=9e-14,
Organism=Drosophila melanogaster, GI24644950, Length=299, Percent_Identity=28.0936454849498, Blast_Score=99, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24663317, Length=308, Percent_Identity=27.2727272727273, Blast_Score=90, Evalue=1e-18,
Organism=Drosophila melanogaster, GI20129731, Length=298, Percent_Identity=29.5302013422819, Blast_Score=89, Evalue=3e-18,
Organism=Drosophila melanogaster, GI45553081, Length=284, Percent_Identity=26.4084507042254, Blast_Score=86, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24662789, Length=276, Percent_Identity=25.7246376811594, Blast_Score=76, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24657054, Length=292, Percent_Identity=23.972602739726, Blast_Score=76, Evalue=2e-14,
Organism=Drosophila melanogaster, GI21356425, Length=273, Percent_Identity=23.8095238095238, Blast_Score=75, Evalue=5e-14,
Organism=Drosophila melanogaster, GI281366140, Length=250, Percent_Identity=26, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24662781, Length=285, Percent_Identity=26.3157894736842, Blast_Score=64, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001395
- InterPro:   IPR020471
- InterPro:   IPR023210 [H]

Pfam domain/function: PF00248 Aldo_ket_red [H]

EC number: NA

Molecular weight: Translated: 30349; Mature: 30349

Theoretical pI: Translated: 5.57; Mature: 5.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRTLELAGRTVPVLGQGTWHMGEKGHQRSAEIAGLCLGIEQGLTLIDTAEMYADGAAEEI
CCCCCCCCCEEEEEECCCCCCCCCCCCCCHHHHHEEEEHHHCCEEEEHHHHHHCCCHHHH
VGQAIAGQRDKVFVVSKVYPHNASRAGIPTACEASLRRMNTDYIDLYLLHWPGQYPLTET
HHHHHCCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHCCCCEEEEEEEECCCCCCHHHH
VEAFERLREAGKIGAWGVSNFDVPDMIELNNPRCATNQVLYNPEQRGIEFDLMLWSADRH
HHHHHHHHHHCCCCCCCCCCCCCCCEEECCCCCCCCCCEEECCHHHCEEEEEEEEECCCC
LPLMAYCPIGQAGDLLYHPALHEIAKRHDATPAQISLAWVLRQDNMIAIPKATNPEHIRL
CCEEEECCCCCCCCEEECHHHHHHHHHCCCCCCEEEEEEEEECCCEEEECCCCCCCEEEE
NIAAEQIRLTERDLADIDLAWPAPTRKVPLAMV
EEEHHHEEECCCCCCCEEEECCCCCCCCCEEEC
>Mature Secondary Structure
MRTLELAGRTVPVLGQGTWHMGEKGHQRSAEIAGLCLGIEQGLTLIDTAEMYADGAAEEI
CCCCCCCCCEEEEEECCCCCCCCCCCCCCHHHHHEEEEHHHCCEEEEHHHHHHCCCHHHH
VGQAIAGQRDKVFVVSKVYPHNASRAGIPTACEASLRRMNTDYIDLYLLHWPGQYPLTET
HHHHHCCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHCCCCEEEEEEEECCCCCCHHHH
VEAFERLREAGKIGAWGVSNFDVPDMIELNNPRCATNQVLYNPEQRGIEFDLMLWSADRH
HHHHHHHHHHCCCCCCCCCCCCCCCEEECCCCCCCCCCEEECCHHHCEEEEEEEEECCCC
LPLMAYCPIGQAGDLLYHPALHEIAKRHDATPAQISLAWVLRQDNMIAIPKATNPEHIRL
CCEEEECCCCCCCCEEECHHHHHHHHHCCCCCCEEEEEEEEECCCEEEECCCCCCCEEEE
NIAAEQIRLTERDLADIDLAWPAPTRKVPLAMV
EEEHHHEEECCCCCCCEEEECCCCCCCCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097040; 9278503 [H]