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The map label for this gene is mltD [H]

Identifier: 66045005

GI number: 66045005

Start: 2001324

End: 2002928

Strand: Reverse

Name: mltD [H]

Synonym: Psyr_1760

Alternate gene names: 66045005

Gene position: 2002928-2001324 (Counterclockwise)

Preceding gene: 66045006

Following gene: 66045004

Centisome position: 32.87

GC content: 60.19

Gene sequence:

>1605_bases
ATGTCGTCATCTACCAGCAAGCCTTCACATTCGGACGCATTGACTCGGTTGGCTCAGGCCATGGCCGTCACCGCGAGTGC
CCTTCTGGCCGGTTGCCAGAGCACCGCCAGCGTCGACCCGGCCAGTAATCATCGCGCGCCTAACCTGGCCGCCGGAATCA
AGCAAAAACCGATTTTTCTGTCACACAAGCCTGCCACCCCTCTCGCACCTCCACAGGATGTCTGGGAGCGCATGCGTCAG
GGCTTTCAACTGCAACAAGGCAACGATCAGAACCCCCGCATCGATCAACAGCGCCTGTGGTTCGCCAACAACCCTTCCTT
TCTGGAAAACGCTGGCGAGCGCGGCAGCCTGTACATGCACTACATCGTCGAGCGCCTTGAAGAACGCAACATGCCGCTGG
AACTGGCCCTGCTTCCGGTTATCGAAAGCGCCTACAACCCGATGGCCGTTTCACGCAGCCAGGCGGTCGGCCTCTGGCAG
TTCATCCCGTCGACCGGGCGTTACTTCAACCTGCGCCAGACCAGCTTTTATGACGGACGTCGCGACATCCAGGCCTCGAC
TGTTGCGGCACTGGATTACCTGACCCGCCTGCACGACATGTTCAACGGTGACTGGCTGCTGGCCCTGGCAGCCTACAATG
CCGGCGAAGGCACGGTGAGCCGCGCCATCGAGCGCAATGACAAGCTCAACCTGCCGACCGACTACTGGAACCTGCCGCTG
CCGCAGGAAACCCGCGATTACGTGCCCAAGCTGCTGGCCCTGTCCCAGGTTGTATTGTCGCCGGAAGCTTATGGCGTCAA
TCTGAACCCGATCGCCAACCAGCCTTACTTCGAAGTCGTCGAGCTCAACAAGCGTGTCGACCTGTCCAAAGTGGCCAGCG
CCGCCGACATCGACGAAGACGAGCTTATCCAGCTCAACCCCGCCTACAAGAAGCGCCTGACCGTCGATGGCCCCCAGCAC
CTGCTGGTGCCCACCTCCAAGGCGCAACTGCTGACGGCCAGCCTGCTGACCATGAAACAGGAAGAGCTCGTCGCCTGGCA
GCCTTACCGGGTGCGCAAGGGCGACACTCTGGAAAGCCTGGCAAGTCGTTATCAGGTCACGGTCAGCTCGCTCAAGGGCA
ATAACAAGCTGTCCGGCAACCGCCTGAAGGTCGGTCAGTCGCTGAGCATACCGGTCAAGCCGGGCATGCAGAATTCACAG
CCTGTGTTCGAAGCGCTGGCCAGCAACGACAAGCCGTCACGTACCCGCAGCTACAAAGTGCGCAGCGGTGACAACCTGAC
CAACATCGCGCAAGCCAACAAGGTTGACGTCGAAGACCTGCAACGCTGGAACAAGCTGACCGGCAAGAAGCTCAAGGTCG
GCCAGACGCTGGTCATGCAGGACACCAGCAAACCTGCCGGTAAAGCCGCCAGCAAGGCGGTCGCCAGCACCAGCGCCAAG
GATGGCGAAAAGAAATCCATGCAGTACAAGATCCAGAAAGGTGACTCGATGTACCTGGTCGCCAAGCGCTTCAACGTCGA
GATGCAACATCTCAAGCGCTGGAATCCACGTAGCGGGCAGGCCCTCAAACCCGGCCAGACCCTGACCGTTTACCTGCCAC
ATTGA

Upstream 100 bases:

>100_bases
ACCCGACCCTAAAACCGCAATAAAATTCTGAAAGGTTGACCGACGGCAGGCTGCTTCCTAGAATCGCCCGACATTTTCGT
CTGGATGTACCCCCCGAACA

Downstream 100 bases:

>100_bases
TGAGCTCGGCCACTTGCCCTTCGATAACCTTGGGGCGGGTGGCCGATGCAGCCCCGGCAACGCCATCACCTGCAGTCTTT
TTCCAGCTGGAACAAGCTGT

Product: peptidoglycan-binding LysM:SLT:MLTD_N

Products: 1,6-Anhydrobond In The Muramic Acid Residue [C]

Alternate protein names: Murein hydrolase D; Regulatory protein dniR [H]

Number of amino acids: Translated: 534; Mature: 533

Protein sequence:

>534_residues
MSSSTSKPSHSDALTRLAQAMAVTASALLAGCQSTASVDPASNHRAPNLAAGIKQKPIFLSHKPATPLAPPQDVWERMRQ
GFQLQQGNDQNPRIDQQRLWFANNPSFLENAGERGSLYMHYIVERLEERNMPLELALLPVIESAYNPMAVSRSQAVGLWQ
FIPSTGRYFNLRQTSFYDGRRDIQASTVAALDYLTRLHDMFNGDWLLALAAYNAGEGTVSRAIERNDKLNLPTDYWNLPL
PQETRDYVPKLLALSQVVLSPEAYGVNLNPIANQPYFEVVELNKRVDLSKVASAADIDEDELIQLNPAYKKRLTVDGPQH
LLVPTSKAQLLTASLLTMKQEELVAWQPYRVRKGDTLESLASRYQVTVSSLKGNNKLSGNRLKVGQSLSIPVKPGMQNSQ
PVFEALASNDKPSRTRSYKVRSGDNLTNIAQANKVDVEDLQRWNKLTGKKLKVGQTLVMQDTSKPAGKAASKAVASTSAK
DGEKKSMQYKIQKGDSMYLVAKRFNVEMQHLKRWNPRSGQALKPGQTLTVYLPH

Sequences:

>Translated_534_residues
MSSSTSKPSHSDALTRLAQAMAVTASALLAGCQSTASVDPASNHRAPNLAAGIKQKPIFLSHKPATPLAPPQDVWERMRQ
GFQLQQGNDQNPRIDQQRLWFANNPSFLENAGERGSLYMHYIVERLEERNMPLELALLPVIESAYNPMAVSRSQAVGLWQ
FIPSTGRYFNLRQTSFYDGRRDIQASTVAALDYLTRLHDMFNGDWLLALAAYNAGEGTVSRAIERNDKLNLPTDYWNLPL
PQETRDYVPKLLALSQVVLSPEAYGVNLNPIANQPYFEVVELNKRVDLSKVASAADIDEDELIQLNPAYKKRLTVDGPQH
LLVPTSKAQLLTASLLTMKQEELVAWQPYRVRKGDTLESLASRYQVTVSSLKGNNKLSGNRLKVGQSLSIPVKPGMQNSQ
PVFEALASNDKPSRTRSYKVRSGDNLTNIAQANKVDVEDLQRWNKLTGKKLKVGQTLVMQDTSKPAGKAASKAVASTSAK
DGEKKSMQYKIQKGDSMYLVAKRFNVEMQHLKRWNPRSGQALKPGQTLTVYLPH
>Mature_533_residues
SSSTSKPSHSDALTRLAQAMAVTASALLAGCQSTASVDPASNHRAPNLAAGIKQKPIFLSHKPATPLAPPQDVWERMRQG
FQLQQGNDQNPRIDQQRLWFANNPSFLENAGERGSLYMHYIVERLEERNMPLELALLPVIESAYNPMAVSRSQAVGLWQF
IPSTGRYFNLRQTSFYDGRRDIQASTVAALDYLTRLHDMFNGDWLLALAAYNAGEGTVSRAIERNDKLNLPTDYWNLPLP
QETRDYVPKLLALSQVVLSPEAYGVNLNPIANQPYFEVVELNKRVDLSKVASAADIDEDELIQLNPAYKKRLTVDGPQHL
LVPTSKAQLLTASLLTMKQEELVAWQPYRVRKGDTLESLASRYQVTVSSLKGNNKLSGNRLKVGQSLSIPVKPGMQNSQP
VFEALASNDKPSRTRSYKVRSGDNLTNIAQANKVDVEDLQRWNKLTGKKLKVGQTLVMQDTSKPAGKAASKAVASTSAKD
GEKKSMQYKIQKGDSMYLVAKRFNVEMQHLKRWNPRSGQALKPGQTLTVYLPH

Specific function: Murein-degrading enzyme. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG0741

COG function: function code M; Soluble lytic murein transglycosylase and related regulatory proteins (some contain LysM/invasin domains)

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 LysM repeats [H]

Homologues:

Organism=Escherichia coli, GI1786405, Length=461, Percent_Identity=41.2147505422993, Blast_Score=311, Evalue=5e-86,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008258
- InterPro:   IPR010511
- InterPro:   IPR018392
- InterPro:   IPR002482
- InterPro:   IPR000189 [H]

Pfam domain/function: PF01476 LysM; PF06474 MLTD_N; PF01464 SLT [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 59397; Mature: 59266

Theoretical pI: Translated: 10.11; Mature: 10.11

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS00922 TRANSGLYCOSYLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSSTSKPSHSDALTRLAQAMAVTASALLAGCQSTASVDPASNHRAPNLAAGIKQKPIFL
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHCCCCCCEEE
SHKPATPLAPPQDVWERMRQGFQLQQGNDQNPRIDQQRLWFANNPSFLENAGERGSLYMH
ECCCCCCCCCHHHHHHHHHCCCEECCCCCCCCCCCHHHEEECCCCHHHHCCCCCCCCHHH
YIVERLEERNMPLELALLPVIESAYNPMAVSRSQAVGLWQFIPSTGRYFNLRQTSFYDGR
HHHHHHHHCCCCEEEHHHHHHHHCCCCCCCCCHHHCCHHEECCCCCCEEEEECCCCCCCC
RDIQASTVAALDYLTRLHDMFNGDWLLALAAYNAGEGTVSRAIERNDKLNLPTDYWNLPL
CCHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHCCCCCCCCCCCCCCCC
PQETRDYVPKLLALSQVVLSPEAYGVNLNPIANQPYFEVVELNKRVDLSKVASAADIDED
CHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEHHHHCCCCCHHHHHHHCCCCCC
ELIQLNPAYKKRLTVDGPQHLLVPTSKAQLLTASLLTMKQEELVAWQPYRVRKGDTLESL
CEEEECHHHHHEEECCCCCEEEEECCHHHHHHHHHHHHHHHHHEECCCEEECCCCHHHHH
ASRYQVTVSSLKGNNKLSGNRLKVGQSLSIPVKPGMQNSQPVFEALASNDKPSRTRSYKV
HHHHEEEHHHHCCCCCCCCCEEECCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCEEEE
RSGDNLTNIAQANKVDVEDLQRWNKLTGKKLKVGQTLVMQDTSKPAGKAASKAVASTSAK
CCCCCHHHHHHCCCCCHHHHHHHHHHCCCEEECCCEEEEECCCCCCHHHHHHHHHHCCCC
DGEKKSMQYKIQKGDSMYLVAKRFNVEMQHLKRWNPRSGQALKPGQTLTVYLPH
CCCHHCEEEEEECCCEEEEEEECCCCCHHHHHCCCCCCCCCCCCCCEEEEEECC
>Mature Secondary Structure 
SSSTSKPSHSDALTRLAQAMAVTASALLAGCQSTASVDPASNHRAPNLAAGIKQKPIFL
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHCCCCCCEEE
SHKPATPLAPPQDVWERMRQGFQLQQGNDQNPRIDQQRLWFANNPSFLENAGERGSLYMH
ECCCCCCCCCHHHHHHHHHCCCEECCCCCCCCCCCHHHEEECCCCHHHHCCCCCCCCHHH
YIVERLEERNMPLELALLPVIESAYNPMAVSRSQAVGLWQFIPSTGRYFNLRQTSFYDGR
HHHHHHHHCCCCEEEHHHHHHHHCCCCCCCCCHHHCCHHEECCCCCCEEEEECCCCCCCC
RDIQASTVAALDYLTRLHDMFNGDWLLALAAYNAGEGTVSRAIERNDKLNLPTDYWNLPL
CCHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHCCCCCCCCCCCCCCCC
PQETRDYVPKLLALSQVVLSPEAYGVNLNPIANQPYFEVVELNKRVDLSKVASAADIDED
CHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEHHHHCCCCCHHHHHHHCCCCCC
ELIQLNPAYKKRLTVDGPQHLLVPTSKAQLLTASLLTMKQEELVAWQPYRVRKGDTLESL
CEEEECHHHHHEEECCCCCEEEEECCHHHHHHHHHHHHHHHHHEECCCEEECCCCHHHHH
ASRYQVTVSSLKGNNKLSGNRLKVGQSLSIPVKPGMQNSQPVFEALASNDKPSRTRSYKV
HHHHEEEHHHHCCCCCCCCCEEECCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCEEEE
RSGDNLTNIAQANKVDVEDLQRWNKLTGKKLKVGQTLVMQDTSKPAGKAASKAVASTSAK
CCCCCHHHHHHCCCCCHHHHHHHHHHCCCEEECCCEEEEECCCCCCHHHHHHHHHHCCCC
DGEKKSMQYKIQKGDSMYLVAKRFNVEMQHLKRWNPRSGQALKPGQTLTVYLPH
CCCHHCEEEEEECCCEEEEEEECCCCCHHHHHCCCCCCCCCCCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond Between N-Acetylmuramic Acid And N-Acetylglucosamine Residues, Thereby Conserving The Energy In A Newly Synthesized 1,6-Anhydrobond In The Muramic Acid Residue. [C]

General reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]