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The map label for this gene is emrB [H]

Identifier: 66044976

GI number: 66044976

Start: 1965072

End: 1966610

Strand: Direct

Name: emrB [H]

Synonym: Psyr_1731

Alternate gene names: 66044976

Gene position: 1965072-1966610 (Clockwise)

Preceding gene: 66044975

Following gene: 66044979

Centisome position: 32.25

GC content: 62.05

Gene sequence:

>1539_bases
ATGAGCAACAACGCCCCCGCTTCTTTTACCCCGCCAAGCCTGTTGCTGTGCACCATCGGTCTGTCGCTGGCGACCTTCAT
GCAGGTGCTCGATACCACGATTGCCAACGTGGCGCTGCCGACCATCGCCGGCAACCTGGGCGTCAGCTCAGAGCAGAGCA
CCTGGGTGATCACCTCGTTTGCGGTCAGCAACGCGATTGCCTTGCCACTGACCGGCTGGCTCAGTCGCCGCTTCGGTGAG
GTCAAGCTGTTCCTGTGGGCCACCATTCTGTTCGTCATCGCTTCGTTTCTGTGCGGCATTTCCCAGTCCATGCCGGAACT
GGTGGGCTTCCGCGCGCTGCAGGGGATGGTCGCCGGGCCGCTGTATCCGATGAGTCAGACCCTGCTGATTGCGGTGTACC
CGCCCGCCAAAAGGGGCATGGCCCTGGCGTTGCTGGCAATGGTGACGGTGGTGGCGCCGATTGCCGGGCCGATCCTCGGT
GGCTGGATCACCGACAGTTACAGCTGGCCGTGGATCTTCTTCATCAACATTCCCATCGGTCTGTTCGCTGTGCTGGTAGT
GCGTAGCCAGATGTCCAAAAGACCGGTCACGACCGCTCGTCAACCGCTGGACTACATTGGCCTGCTGGCGCTGATCGTCG
GTGTGGGCGCGCTGCAGGTAGTGCTCGACAAGGGTAATGATCTGGACTGGTTCGAGTCGAACTTCATCATCTTCGGCTCG
CTGATTTCGCTGGTGGCGCTGGTTTTCTTCGTCATCTGGGAAATGACCGACAAGCACCCGATCGTCAACCTGCGCCTGTT
TGCCTACCGCAATTTCCGCATCGGCACGCTGGTCATGATCGGCGGCTATTCCGGCTTCTTCGGCATCAACCTGATATTGC
CGCAGTGGTTGCAGACCCAGATGGGCTATACCGCGACCTGGGCGGGGCTGGCTGTCGCGCCGATTGGCATCCTGCCAGTA
CTGATGTCGCCCTTTGTCGGCAAGTACGCGCACAAGTTCGACCTGCGTCTGCTGGCCGGGCTGGCGTTCCTGGCGATGGG
GCTGAGCTGCTTCATGCGTGCAGGCTTCAACACCGACGTGGATTTCGAACACGTAGCGATGGTGCAGCTGTTCATGGGCA
TCGGCGTGGCACTGTTCTTCATGCCGACCCTGAGCATTCTGCTGTCCGACCTGCCGCCCGATCAGATCGCCGATGGCTCC
GGACTGGCAACCTTCCTGCGCACCCTGGGAGGCAGCTTCGCAGCGTCGCTGACCACCTGGATCTGGATCCGCCGCGCCAA
CCAGCATCATGCCTACCTGAGCGAAAACATCAGCACCTTCGACCCCGCCACCCGCCACGCCCTCGACAGCCTGGGCGGCG
CCAGCCCGCAAGCCTACGCGCAACTGGAGCAGACCCTCAACGCACAGGCCTACATGATGTCCACCGTGGATTACTTCTAC
CTGCTGGGCTGGATATTCGCCGGACTGATCCTGCTCGTCTGGCTGGCCAAACCCCCATTCGCCGCCAAGGCCGGGCCTGC
GGCAGGCGGCGGGCACTAG

Upstream 100 bases:

>100_bases
CAGTGCGCCCGGTGGCAAGACTGCCGTTCAGCGCTGAACCGCTAATCCGTGAGCCCCGGCCTGCTGGCCGGGGTGTCGCA
TTCGTCTTCAGGGATTCGCG

Downstream 100 bases:

>100_bases
TTTCTTGTGCGCCTATCTGAACCGTGTCTATGGCCAAACTGCTCTAAAGTGCTTTTTCAACCACGATGGCACAGACGACG
CAGAACCGCTGTGTGACGCA

Product: EmrB/QacA family drug resistance transporter

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 512; Mature: 511

Protein sequence:

>512_residues
MSNNAPASFTPPSLLLCTIGLSLATFMQVLDTTIANVALPTIAGNLGVSSEQSTWVITSFAVSNAIALPLTGWLSRRFGE
VKLFLWATILFVIASFLCGISQSMPELVGFRALQGMVAGPLYPMSQTLLIAVYPPAKRGMALALLAMVTVVAPIAGPILG
GWITDSYSWPWIFFINIPIGLFAVLVVRSQMSKRPVTTARQPLDYIGLLALIVGVGALQVVLDKGNDLDWFESNFIIFGS
LISLVALVFFVIWEMTDKHPIVNLRLFAYRNFRIGTLVMIGGYSGFFGINLILPQWLQTQMGYTATWAGLAVAPIGILPV
LMSPFVGKYAHKFDLRLLAGLAFLAMGLSCFMRAGFNTDVDFEHVAMVQLFMGIGVALFFMPTLSILLSDLPPDQIADGS
GLATFLRTLGGSFAASLTTWIWIRRANQHHAYLSENISTFDPATRHALDSLGGASPQAYAQLEQTLNAQAYMMSTVDYFY
LLGWIFAGLILLVWLAKPPFAAKAGPAAGGGH

Sequences:

>Translated_512_residues
MSNNAPASFTPPSLLLCTIGLSLATFMQVLDTTIANVALPTIAGNLGVSSEQSTWVITSFAVSNAIALPLTGWLSRRFGE
VKLFLWATILFVIASFLCGISQSMPELVGFRALQGMVAGPLYPMSQTLLIAVYPPAKRGMALALLAMVTVVAPIAGPILG
GWITDSYSWPWIFFINIPIGLFAVLVVRSQMSKRPVTTARQPLDYIGLLALIVGVGALQVVLDKGNDLDWFESNFIIFGS
LISLVALVFFVIWEMTDKHPIVNLRLFAYRNFRIGTLVMIGGYSGFFGINLILPQWLQTQMGYTATWAGLAVAPIGILPV
LMSPFVGKYAHKFDLRLLAGLAFLAMGLSCFMRAGFNTDVDFEHVAMVQLFMGIGVALFFMPTLSILLSDLPPDQIADGS
GLATFLRTLGGSFAASLTTWIWIRRANQHHAYLSENISTFDPATRHALDSLGGASPQAYAQLEQTLNAQAYMMSTVDYFY
LLGWIFAGLILLVWLAKPPFAAKAGPAAGGGH
>Mature_511_residues
SNNAPASFTPPSLLLCTIGLSLATFMQVLDTTIANVALPTIAGNLGVSSEQSTWVITSFAVSNAIALPLTGWLSRRFGEV
KLFLWATILFVIASFLCGISQSMPELVGFRALQGMVAGPLYPMSQTLLIAVYPPAKRGMALALLAMVTVVAPIAGPILGG
WITDSYSWPWIFFINIPIGLFAVLVVRSQMSKRPVTTARQPLDYIGLLALIVGVGALQVVLDKGNDLDWFESNFIIFGSL
ISLVALVFFVIWEMTDKHPIVNLRLFAYRNFRIGTLVMIGGYSGFFGINLILPQWLQTQMGYTATWAGLAVAPIGILPVL
MSPFVGKYAHKFDLRLLAGLAFLAMGLSCFMRAGFNTDVDFEHVAMVQLFMGIGVALFFMPTLSILLSDLPPDQIADGSG
LATFLRTLGGSFAASLTTWIWIRRANQHHAYLSENISTFDPATRHALDSLGGASPQAYAQLEQTLNAQAYMMSTVDYFYL
LGWIFAGLILLVWLAKPPFAAKAGPAAGGGH

Specific function: Translocase that confers resistance to substances of high hydrophobicity [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1789042, Length=492, Percent_Identity=51.4227642276423, Blast_Score=494, Evalue=1e-141,
Organism=Escherichia coli, GI1788710, Length=507, Percent_Identity=43.9842209072978, Blast_Score=434, Evalue=1e-123,
Organism=Escherichia coli, GI1790195, Length=413, Percent_Identity=30.5084745762712, Blast_Score=162, Evalue=4e-41,
Organism=Escherichia coli, GI1788392, Length=411, Percent_Identity=28.9537712895377, Blast_Score=126, Evalue=3e-30,
Organism=Escherichia coli, GI87081983, Length=408, Percent_Identity=24.2647058823529, Blast_Score=120, Evalue=2e-28,
Organism=Saccharomyces cerevisiae, GI6325455, Length=181, Percent_Identity=31.4917127071823, Blast_Score=84, Evalue=7e-17,
Organism=Saccharomyces cerevisiae, GI6322958, Length=209, Percent_Identity=26.7942583732057, Blast_Score=79, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6321663, Length=150, Percent_Identity=28.6666666666667, Blast_Score=76, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 55438; Mature: 55307

Theoretical pI: Translated: 8.07; Mature: 8.07

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNNAPASFTPPSLLLCTIGLSLATFMQVLDTTIANVALPTIAGNLGVSSEQSTWVITSF
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEH
AVSNAIALPLTGWLSRRFGEVKLFLWATILFVIASFLCGISQSMPELVGFRALQGMVAGP
HHHCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
LYPMSQTLLIAVYPPAKRGMALALLAMVTVVAPIAGPILGGWITDSYSWPWIFFINIPIG
CCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCHH
LFAVLVVRSQMSKRPVTTARQPLDYIGLLALIVGVGALQVVLDKGNDLDWFESNFIIFGS
HHHHHHHHHHHHCCCCCHHHCCHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCHHHHHH
LISLVALVFFVIWEMTDKHPIVNLRLFAYRNFRIGTLVMIGGYSGFFGINLILPQWLQTQ
HHHHHHHHHHHHHHHCCCCCEEEEEEEEEECCEEEEEEEECCCCCHHHHHHHHHHHHHHH
MGYTATWAGLAVAPIGILPVLMSPFVGKYAHKFDLRLLAGLAFLAMGLSCFMRAGFNTDV
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
DFEHVAMVQLFMGIGVALFFMPTLSILLSDLPPDQIADGSGLATFLRTLGGSFAASLTTW
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCCCCHHHHHHHHHCCHHHHHHHHH
IWIRRANQHHAYLSENISTFDPATRHALDSLGGASPQAYAQLEQTLNAQAYMMSTVDYFY
HHHEECCCCHHHHHCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHCHHHHHHHHHHHHH
LLGWIFAGLILLVWLAKPPFAAKAGPAAGGGH
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SNNAPASFTPPSLLLCTIGLSLATFMQVLDTTIANVALPTIAGNLGVSSEQSTWVITSF
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEH
AVSNAIALPLTGWLSRRFGEVKLFLWATILFVIASFLCGISQSMPELVGFRALQGMVAGP
HHHCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
LYPMSQTLLIAVYPPAKRGMALALLAMVTVVAPIAGPILGGWITDSYSWPWIFFINIPIG
CCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCHH
LFAVLVVRSQMSKRPVTTARQPLDYIGLLALIVGVGALQVVLDKGNDLDWFESNFIIFGS
HHHHHHHHHHHHCCCCCHHHCCHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCHHHHHH
LISLVALVFFVIWEMTDKHPIVNLRLFAYRNFRIGTLVMIGGYSGFFGINLILPQWLQTQ
HHHHHHHHHHHHHHHCCCCCEEEEEEEEEECCEEEEEEEECCCCCHHHHHHHHHHHHHHH
MGYTATWAGLAVAPIGILPVLMSPFVGKYAHKFDLRLLAGLAFLAMGLSCFMRAGFNTDV
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
DFEHVAMVQLFMGIGVALFFMPTLSILLSDLPPDQIADGSGLATFLRTLGGSFAASLTTW
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCCCCHHHHHHHHHCCHHHHHHHHH
IWIRRANQHHAYLSENISTFDPATRHALDSLGGASPQAYAQLEQTLNAQAYMMSTVDYFY
HHHEECCCCHHHHHCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHCHHHHHHHHHHHHH
LLGWIFAGLILLVWLAKPPFAAKAGPAAGGGH
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]