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The map label for this gene is arsB [H]

Identifier: 66044927

GI number: 66044927

Start: 1890072

End: 1891352

Strand: Direct

Name: arsB [H]

Synonym: Psyr_1682

Alternate gene names: 66044927

Gene position: 1890072-1891352 (Clockwise)

Preceding gene: 66044926

Following gene: 66044929

Centisome position: 31.02

GC content: 60.81

Gene sequence:

>1281_bases
ATGCTGATAGCAACGCTGATTTTCCTGTTCACCATCACGCTGGTCATCTGGCAACCCAAGGGCCTGGGCGTAGGCTGGAG
TGCCGTATTGGGGGCAATTCTGGCGTTGCTGACAGGCGTCGTGCACGTCAGTGACATCGCTGTGGTGTGGGGCATCATCT
GGAATGCCACGGCGACGTTCATTTCGCTGATCATCATCACGCTGCTGCTCGATGAGGCCGGTTTCTTCGCCTGGGCAGCG
CTGCATGTGGCGCGCTGGGGCAAGGGCAACGGGCGCCGTTTGTTTGCCCTGTTCGTGCTATTCGGTGCGCTGGTGTCCGC
CTTGTTCGCCAACGATGGCGCGGTGCTGATCCTCACCCCGATCGTCATTGCCATGATGCTGGCGTTGCGTTTCTCGCCTG
CGTCGACGCTGGCGTTCGTCATGGCGGCCGGTTTCATCGCCGATACGGCCAGCCTGCCGCTGGTGGTCTCGAACCTGGTG
AACATCGTGTCGGCGGATTACTTCAAGATCGGTTTCAACCGTTACGCAGCCGTCATGGTGCCGGTGAATCTGGCCAGTGT
CGCCGCCACCCTGGCCGTGCTGATGTGGTATTTCCGTCGGGATATTCCCGCCGAGTTCGAGCCCGAACAGCTGGACAAGC
CGGAAACGGTCATCCATGACCGCGCCACGTTTCTGGCCGGTTGGGCGGTGCTGGTGATTCTGCTGGTGGGCTGTTTCGCC
CTTGAACCGCTGGGCATCCCGATCAGCGCGATCTCGTCGGTGTGTGCAGTGTTGCTGCTGGCCATCGCCGCCAAAGGGCA
TACGATCAAGACCCGCAAGGTGATGAAAGAAGCACCGTGGCACATCGTGGTCTTTTCGCTGGGCATGTACCTGGTGGTCT
ATGGCCTGCGTAACGCCGGGCTGACCGATTACCTGGCCAATCTGCTGGACTGGTTCGCGGGCTATGGCATCTGGGGCGCG
GCGATGGGCACCGGCGTGATGACTGCGTTCCTGTCTTCGATCATGAACAACATGCCCACGGTACTCATCGGGCTGCTGTC
CATCGACGCAAGTCATGCCAGCGGTGCGATCCAGGAAGCCATGATCTACGCCAACGTGATCGGTAGCGACCTCGGCCCGA
AAATCACCCCCATCGGCAGCCTGGCCACGCTGCTCTGGCTGCATGTGCTGCAGCGCAAGGGCATCACTATCAGCTGGGGC
TACTACTTCAAGGTCGGTATCGTGCTGACCGTGCCTGTGCTGCTGGTCACACTGGCTGCGCTGGCATTGCGGTTGAGTTG
A

Upstream 100 bases:

>100_bases
GTTATCTGGCTGACAGCCACGGCATCCTGTTCGTCTACACGCTGTGCTCGTACCTGCCGCTGCTCGGTATCCTCGCGATT
CTTCTGCCTCGGACCAAATA

Downstream 100 bases:

>100_bases
GCGGATGACTGGTATCGGCGAGGTTCGGCCACATGTCCGACACCAGAAACAGCCGTTCGGCTTCTTCCCAGTGGCCCTCG
GCATTCTCCCTGAGGCGCAC

Product: arsenical pump membrane protein

Products: ADP; phosphate; arsenite [Periplasm] [C]

Alternate protein names: Arsenic efflux pump protein [H]

Number of amino acids: Translated: 426; Mature: 426

Protein sequence:

>426_residues
MLIATLIFLFTITLVIWQPKGLGVGWSAVLGAILALLTGVVHVSDIAVVWGIIWNATATFISLIIITLLLDEAGFFAWAA
LHVARWGKGNGRRLFALFVLFGALVSALFANDGAVLILTPIVIAMMLALRFSPASTLAFVMAAGFIADTASLPLVVSNLV
NIVSADYFKIGFNRYAAVMVPVNLASVAATLAVLMWYFRRDIPAEFEPEQLDKPETVIHDRATFLAGWAVLVILLVGCFA
LEPLGIPISAISSVCAVLLLAIAAKGHTIKTRKVMKEAPWHIVVFSLGMYLVVYGLRNAGLTDYLANLLDWFAGYGIWGA
AMGTGVMTAFLSSIMNNMPTVLIGLLSIDASHASGAIQEAMIYANVIGSDLGPKITPIGSLATLLWLHVLQRKGITISWG
YYFKVGIVLTVPVLLVTLAALALRLS

Sequences:

>Translated_426_residues
MLIATLIFLFTITLVIWQPKGLGVGWSAVLGAILALLTGVVHVSDIAVVWGIIWNATATFISLIIITLLLDEAGFFAWAA
LHVARWGKGNGRRLFALFVLFGALVSALFANDGAVLILTPIVIAMMLALRFSPASTLAFVMAAGFIADTASLPLVVSNLV
NIVSADYFKIGFNRYAAVMVPVNLASVAATLAVLMWYFRRDIPAEFEPEQLDKPETVIHDRATFLAGWAVLVILLVGCFA
LEPLGIPISAISSVCAVLLLAIAAKGHTIKTRKVMKEAPWHIVVFSLGMYLVVYGLRNAGLTDYLANLLDWFAGYGIWGA
AMGTGVMTAFLSSIMNNMPTVLIGLLSIDASHASGAIQEAMIYANVIGSDLGPKITPIGSLATLLWLHVLQRKGITISWG
YYFKVGIVLTVPVLLVTLAALALRLS
>Mature_426_residues
MLIATLIFLFTITLVIWQPKGLGVGWSAVLGAILALLTGVVHVSDIAVVWGIIWNATATFISLIIITLLLDEAGFFAWAA
LHVARWGKGNGRRLFALFVLFGALVSALFANDGAVLILTPIVIAMMLALRFSPASTLAFVMAAGFIADTASLPLVVSNLV
NIVSADYFKIGFNRYAAVMVPVNLASVAATLAVLMWYFRRDIPAEFEPEQLDKPETVIHDRATFLAGWAVLVILLVGCFA
LEPLGIPISAISSVCAVLLLAIAAKGHTIKTRKVMKEAPWHIVVFSLGMYLVVYGLRNAGLTDYLANLLDWFAGYGIWGA
AMGTGVMTAFLSSIMNNMPTVLIGLLSIDASHASGAIQEAMIYANVIGSDLGPKITPIGSLATLLWLHVLQRKGITISWG
YYFKVGIVLTVPVLLVTLAALALRLS

Specific function: Involved in arsenical resistance. Thought to form the channel of an arsenite pump [H]

COG id: COG1055

COG function: function code P; Na+/H+ antiporter NhaD and related arsenite permeases

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ArsB family [H]

Homologues:

Organism=Escherichia coli, GI87082277, Length=426, Percent_Identity=70.18779342723, Blast_Score=578, Evalue=1e-166,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000802 [H]

Pfam domain/function: PF02040 ArsB [H]

EC number: NA

Molecular weight: Translated: 45627; Mature: 45627

Theoretical pI: Translated: 8.96; Mature: 8.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLIATLIFLFTITLVIWQPKGLGVGWSAVLGAILALLTGVVHVSDIAVVWGIIWNATATF
CHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ISLIIITLLLDEAGFFAWAALHVARWGKGNGRRLFALFVLFGALVSALFANDGAVLILTP
HHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEHHHH
IVIAMMLALRFSPASTLAFVMAAGFIADTASLPLVVSNLVNIVSADYFKIGFNRYAAVMV
HHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEE
PVNLASVAATLAVLMWYFRRDIPAEFEPEQLDKPETVIHDRATFLAGWAVLVILLVGCFA
CHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LEPLGIPISAISSVCAVLLLAIAAKGHTIKTRKVMKEAPWHIVVFSLGMYLVVYGLRNAG
HCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCC
LTDYLANLLDWFAGYGIWGAAMGTGVMTAFLSSIMNNMPTVLIGLLSIDASHASGAIQEA
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCHHHHHHHHHH
MIYANVIGSDLGPKITPIGSLATLLWLHVLQRKGITISWGYYFKVGIVLTVPVLLVTLAA
HHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEECHHHHHHHHHHHHHHHHHHHH
LALRLS
HHHHCC
>Mature Secondary Structure
MLIATLIFLFTITLVIWQPKGLGVGWSAVLGAILALLTGVVHVSDIAVVWGIIWNATATF
CHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ISLIIITLLLDEAGFFAWAALHVARWGKGNGRRLFALFVLFGALVSALFANDGAVLILTP
HHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEHHHH
IVIAMMLALRFSPASTLAFVMAAGFIADTASLPLVVSNLVNIVSADYFKIGFNRYAAVMV
HHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEE
PVNLASVAATLAVLMWYFRRDIPAEFEPEQLDKPETVIHDRATFLAGWAVLVILLVGCFA
CHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LEPLGIPISAISSVCAVLLLAIAAKGHTIKTRKVMKEAPWHIVVFSLGMYLVVYGLRNAG
HCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCC
LTDYLANLLDWFAGYGIWGAAMGTGVMTAFLSSIMNNMPTVLIGLLSIDASHASGAIQEA
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCHHHHHHHHHH
MIYANVIGSDLGPKITPIGSLATLLWLHVLQRKGITISWGYYFKVGIVLTVPVLLVTLAA
HHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEECHHHHHHHHHHHHHHHHHHHH
LALRLS
HHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; arsenite [Cytoplasm]; H2O [C]

Specific reaction: ATP + arsenite [Cytoplasm] + H2O = ADP + phosphate + arsenite [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9006011 [H]