Click here to switch to the map view.

The map label for this gene is holB [H]

Identifier: 66044898

GI number: 66044898

Start: 1857645

End: 1858631

Strand: Direct

Name: holB [H]

Synonym: Psyr_1653

Alternate gene names: 66044898

Gene position: 1857645-1858631 (Clockwise)

Preceding gene: 66044897

Following gene: 66044899

Centisome position: 30.48

GC content: 61.6

Gene sequence:

>987_bases
GTGGCTGAAGCCTACCCCTGGCAGGCTTCGCTCTGGCAGCAGATGGCCGGTCGCGCCCAGCACGCTCACGCCTATCTGCT
GCACGGGCCGGCGGGTATCGGCAAGCGCGCTCTGGCTGAACGACTGATGGCCAGCCTGTTGTGCAAGACCCCTGAAGGGC
TTGATGCCTGTGGACACTGCAAGTCCTGTCTGCTGCTGGCGGCTGGCAGCCACCCTGACAACTACGTGCTCGAGCCGGAA
GAGGCGGACAAGCCGATCAAGGTCGATCAGGTGCGTGATCTGGTCAGTTTTGTAGTGCAGACCGCTCAGATGGGCGGGCG
CAAGGTGGTGCTCGTCGAGCCTGTGGAAGCGATGAATACCAACGCGGCCAATGCCTTGCTGAAAAGTCTGGAAGAGCCTT
CAGGCAATACCGTTCTGTTGCTGGTCAGTCATCAACCGAGCCGCTTGCTGCCGACGGTCAAGAGCCGTTGTGTGCAGCAG
GCGTGCCCGTTGCCCGGCGAACAGATGAGCCTCGACTGGCTGAGCGGCGCATTGCCCGATTGCACGCAGGACGAACGCTT
CGAGTTGCTGACGCTGGCCGCCGGATCGCCGCTGGCTGCGGTGGCGCTGCACACGCAGGGCGTTCGCGAACAGCGCGCGC
TGGTGGTAGACGGCGTCAAGAAGCTGCTCAAGCAACAGCAGTCGGCTACCCAGCTGGCCGAGGGCTGGAAGGATATTCCG
CTGTTACTGCTGTTCGACTGGTTCTGTGACTGGTCGCACCTGATCCTGCGCTATCAGATGACCCAGGACGAGGAGGGCCT
TGGCCTGGGTGACATGCGCAAGGTCATTCAATACCTGGCGCAAAAATCTTCTCGCGCCAAAGTGCTGGAAATTCAGGACT
GGATTCTGGCGCAGCGCCAGAAAGTCATGTCCAAGGCCAACCTCAATCGGGTGTTGCTGCTTGAAGCGTTATTGGTGCAG
TGGGCAGGCCTGCCGGGACAGGCGTAG

Upstream 100 bases:

>100_bases
GCCGAGCCTGCGCGCTATCGCCTGGTAGACGCCGCACAGACTCTGGCCGACGTTCAGGCTTGCCTCGACACCTTGCTGCC
TCAGTTGCTGGAGCTGCAAC

Downstream 100 bases:

>100_bases
ACTCTGCGTTATCCGGAACCGTTGAGGTGTTGTCATGAGTCTGCCGCCGAGCACAGGTCCACGTAATGGCATTCTGTCCC
TGACCATCAAGGACAAATCC

Product: DNA polymerase III subunit delta'

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 328; Mature: 327

Protein sequence:

>328_residues
MAEAYPWQASLWQQMAGRAQHAHAYLLHGPAGIGKRALAERLMASLLCKTPEGLDACGHCKSCLLLAAGSHPDNYVLEPE
EADKPIKVDQVRDLVSFVVQTAQMGGRKVVLVEPVEAMNTNAANALLKSLEEPSGNTVLLLVSHQPSRLLPTVKSRCVQQ
ACPLPGEQMSLDWLSGALPDCTQDERFELLTLAAGSPLAAVALHTQGVREQRALVVDGVKKLLKQQQSATQLAEGWKDIP
LLLLFDWFCDWSHLILRYQMTQDEEGLGLGDMRKVIQYLAQKSSRAKVLEIQDWILAQRQKVMSKANLNRVLLLEALLVQ
WAGLPGQA

Sequences:

>Translated_328_residues
MAEAYPWQASLWQQMAGRAQHAHAYLLHGPAGIGKRALAERLMASLLCKTPEGLDACGHCKSCLLLAAGSHPDNYVLEPE
EADKPIKVDQVRDLVSFVVQTAQMGGRKVVLVEPVEAMNTNAANALLKSLEEPSGNTVLLLVSHQPSRLLPTVKSRCVQQ
ACPLPGEQMSLDWLSGALPDCTQDERFELLTLAAGSPLAAVALHTQGVREQRALVVDGVKKLLKQQQSATQLAEGWKDIP
LLLLFDWFCDWSHLILRYQMTQDEEGLGLGDMRKVIQYLAQKSSRAKVLEIQDWILAQRQKVMSKANLNRVLLLEALLVQ
WAGLPGQA
>Mature_327_residues
AEAYPWQASLWQQMAGRAQHAHAYLLHGPAGIGKRALAERLMASLLCKTPEGLDACGHCKSCLLLAAGSHPDNYVLEPEE
ADKPIKVDQVRDLVSFVVQTAQMGGRKVVLVEPVEAMNTNAANALLKSLEEPSGNTVLLLVSHQPSRLLPTVKSRCVQQA
CPLPGEQMSLDWLSGALPDCTQDERFELLTLAAGSPLAAVALHTQGVREQRALVVDGVKKLLKQQQSATQLAEGWKDIPL
LLLFDWFCDWSHLILRYQMTQDEEGLGLGDMRKVIQYLAQKSSRAKVLEIQDWILAQRQKVMSKANLNRVLLLEALLVQW
AGLPGQA

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity [H]

COG id: COG0470

COG function: function code L; ATPase involved in DNA replication

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787341, Length=326, Percent_Identity=33.1288343558282, Blast_Score=128, Evalue=7e-31,
Organism=Escherichia coli, GI1786676, Length=137, Percent_Identity=36.4963503649635, Blast_Score=83, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003959
- InterPro:   IPR004622 [H]

Pfam domain/function: PF00004 AAA [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 36104; Mature: 35973

Theoretical pI: Translated: 6.92; Mature: 6.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEAYPWQASLWQQMAGRAQHAHAYLLHGPAGIGKRALAERLMASLLCKTPEGLDACGHC
CCCCCCCHHHHHHHHHCCHHHCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHH
KSCLLLAAGSHPDNYVLEPEEADKPIKVDQVRDLVSFVVQTAQMGGRKVVLVEPVEAMNT
HHHHHEECCCCCCCCEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHCC
NAANALLKSLEEPSGNTVLLLVSHQPSRLLPTVKSRCVQQACPLPGEQMSLDWLSGALPD
HHHHHHHHHHCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCCCC
CTQDERFELLTLAAGSPLAAVALHTQGVREQRALVVDGVKKLLKQQQSATQLAEGWKDIP
CCCCCCEEEEEEECCCCHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCH
LLLLFDWFCDWSHLILRYQMTQDEEGLGLGDMRKVIQYLAQKSSRAKVLEIQDWILAQRQ
HHHHHHHHHCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHH
KVMSKANLNRVLLLEALLVQWAGLPGQA
HHHHHHCCCHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
AEAYPWQASLWQQMAGRAQHAHAYLLHGPAGIGKRALAERLMASLLCKTPEGLDACGHC
CCCCCCHHHHHHHHHCCHHHCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHH
KSCLLLAAGSHPDNYVLEPEEADKPIKVDQVRDLVSFVVQTAQMGGRKVVLVEPVEAMNT
HHHHHEECCCCCCCCEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHCC
NAANALLKSLEEPSGNTVLLLVSHQPSRLLPTVKSRCVQQACPLPGEQMSLDWLSGALPD
HHHHHHHHHHCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCCCC
CTQDERFELLTLAAGSPLAAVALHTQGVREQRALVVDGVKKLLKQQQSATQLAEGWKDIP
CCCCCCEEEEEEECCCCHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCH
LLLLFDWFCDWSHLILRYQMTQDEEGLGLGDMRKVIQYLAQKSSRAKVLEIQDWILAQRQ
HHHHHHHHHCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHH
KVMSKANLNRVLLLEALLVQWAGLPGQA
HHHHHHCCCHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8550441; 10984043 [H]