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The map label for this gene is nemA [H]

Identifier: 66044802

GI number: 66044802

Start: 1751745

End: 1752866

Strand: Reverse

Name: nemA [H]

Synonym: Psyr_1557

Alternate gene names: 66044802

Gene position: 1752866-1751745 (Counterclockwise)

Preceding gene: 66044804

Following gene: 66044801

Centisome position: 28.77

GC content: 63.37

Gene sequence:

>1122_bases
TTGACTCTGTTCAATGAATTCAAACTCGGCAACACGCCCCTCAGCAACCGCGTCGTCATGGCGCCCATGACCCGCTCGCG
TGCCCCCGAGGATATCGCCGCCGAACAGATCGCGCTGCACTACACCCAGCGCGCCACCGCCGGCCTGATCGTGTCGGAAG
GCACGCCGATCTCACGCGAAGGCCAGGGCTACCTGTTCAATCCAGGCATCTATACCCCGGAGCAGATCCAGGGCTGGAAG
CTGGTGACCGACTCGGTCCACAGCGTCGGCGGTCGCATGTTCGCGCAGCTCTGGCATGTCGGCCGCGTGTCCCACACCTC
GATCCAGATCGACGGCAAAGCACCGGTCAGCGCCACCACCAGACAAGCACAGGGCGCAGTCGCATTCGCCTATGGCGAAG
ACGGCGAACCCGGCTTCGTGCCCACCTCCGCGCCGCGCCCGCTCACCACCGAAGAAGTGGCACGAGTCGTCGAGGATTTC
GCCCAGGCCGCGCAAAATGCGATCGACGCCGGTTTCGACGGCGTGGAAATTCACGGCGCCAATGGATACCTGCTGGAGCA
GTTCCTCAACCCGCTGGTCAACGACCGCGGCGATCAGTACGGCGCGAGCAACCTGCAAGATCGCCTGCGCTTCGTCTTCG
AAGTGGTCGATGCAGCCTGCGCCAGAATCGGTGCCGACCGCGTTGGTATTCGCATCTCGCCCTATGGTCAGTTGTTCGAC
ATGCCGCTCTACCCGGAGATCGACGAAACCTACTCGGCGCTGTGCGCAGGCATGCGCGAACGCGGCATCGCCTATGTACA
CGTCATGGACCAGACGCACTTCTTTATGGCCGGCGAAAGCTCCGCCGCGCAAGAACAGGCACTGCGCAAACTGCTCAAGC
ACTGCAAGGCCGAACTGGGCAACACCGCGCTGATCCTCGCCGGCGACATGACCCGTGAACGCGCTGATGCGCTGCTGGAA
GAAAACCTGATCGACCTTGCTGCGTTTGGCCAGCCTTTCATCGGCAACCCCGACCTGGTCGCCCGCCTGAAAAACGGCTG
GCCCCTGACGACCCCGGACCGTGACACCTACTACGGTGGCGATGCGCACGGCTACATCGACTACGCGCCTTACCGCGCTT
GA

Upstream 100 bases:

>100_bases
GCTACCGCTGACACGTCGCCAGTAATCCCCTGTCAGCCGCCGTCCCAAAGGCTGCTGCCGTCATTGTCCTTTTGATTCCC
CCTCCACAGGAGAAACTGCC

Downstream 100 bases:

>100_bases
TCTAGTGGCCGGGGCGCTGCGTTCGAGCGGTGCCTCGGCAGGGTTCACGGTGAAAGCTGCCTTTCAGGGTAGAAGTGAGG
GGCTGGGCTGGGCTTTCTAC

Product: NADH:flavin oxidoreductase/NADH oxidase

Products: NADP; Cytotoxic compound [C]

Alternate protein names: N-ethylmaleimide reducing enzyme [H]

Number of amino acids: Translated: 373; Mature: 372

Protein sequence:

>373_residues
MTLFNEFKLGNTPLSNRVVMAPMTRSRAPEDIAAEQIALHYTQRATAGLIVSEGTPISREGQGYLFNPGIYTPEQIQGWK
LVTDSVHSVGGRMFAQLWHVGRVSHTSIQIDGKAPVSATTRQAQGAVAFAYGEDGEPGFVPTSAPRPLTTEEVARVVEDF
AQAAQNAIDAGFDGVEIHGANGYLLEQFLNPLVNDRGDQYGASNLQDRLRFVFEVVDAACARIGADRVGIRISPYGQLFD
MPLYPEIDETYSALCAGMRERGIAYVHVMDQTHFFMAGESSAAQEQALRKLLKHCKAELGNTALILAGDMTRERADALLE
ENLIDLAAFGQPFIGNPDLVARLKNGWPLTTPDRDTYYGGDAHGYIDYAPYRA

Sequences:

>Translated_373_residues
MTLFNEFKLGNTPLSNRVVMAPMTRSRAPEDIAAEQIALHYTQRATAGLIVSEGTPISREGQGYLFNPGIYTPEQIQGWK
LVTDSVHSVGGRMFAQLWHVGRVSHTSIQIDGKAPVSATTRQAQGAVAFAYGEDGEPGFVPTSAPRPLTTEEVARVVEDF
AQAAQNAIDAGFDGVEIHGANGYLLEQFLNPLVNDRGDQYGASNLQDRLRFVFEVVDAACARIGADRVGIRISPYGQLFD
MPLYPEIDETYSALCAGMRERGIAYVHVMDQTHFFMAGESSAAQEQALRKLLKHCKAELGNTALILAGDMTRERADALLE
ENLIDLAAFGQPFIGNPDLVARLKNGWPLTTPDRDTYYGGDAHGYIDYAPYRA
>Mature_372_residues
TLFNEFKLGNTPLSNRVVMAPMTRSRAPEDIAAEQIALHYTQRATAGLIVSEGTPISREGQGYLFNPGIYTPEQIQGWKL
VTDSVHSVGGRMFAQLWHVGRVSHTSIQIDGKAPVSATTRQAQGAVAFAYGEDGEPGFVPTSAPRPLTTEEVARVVEDFA
QAAQNAIDAGFDGVEIHGANGYLLEQFLNPLVNDRGDQYGASNLQDRLRFVFEVVDAACARIGADRVGIRISPYGQLFDM
PLYPEIDETYSALCAGMRERGIAYVHVMDQTHFFMAGESSAAQEQALRKLLKHCKAELGNTALILAGDMTRERADALLEE
NLIDLAAFGQPFIGNPDLVARLKNGWPLTTPDRDTYYGGDAHGYIDYAPYRA

Specific function: Catalyzes the reduction of N-ethylmaleimide (NEM) to N- ethylsuccinimide [H]

COG id: COG1902

COG function: function code C; NADH:flavin oxidoreductases, Old Yellow Enzyme family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NADH:flavin oxidoreductase/NADH oxidase family [H]

Homologues:

Organism=Escherichia coli, GI1787939, Length=368, Percent_Identity=43.75, Blast_Score=277, Evalue=7e-76,
Organism=Escherichia coli, GI1789463, Length=351, Percent_Identity=29.6296296296296, Blast_Score=117, Evalue=8e-28,
Organism=Caenorhabditis elegans, GI17565138, Length=299, Percent_Identity=28.4280936454849, Blast_Score=94, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17559804, Length=268, Percent_Identity=29.8507462686567, Blast_Score=93, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI72001454, Length=269, Percent_Identity=30.8550185873606, Blast_Score=92, Evalue=6e-19,
Organism=Caenorhabditis elegans, GI17564188, Length=264, Percent_Identity=29.1666666666667, Blast_Score=91, Evalue=7e-19,
Organism=Caenorhabditis elegans, GI17559802, Length=264, Percent_Identity=26.8939393939394, Blast_Score=90, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17540738, Length=209, Percent_Identity=30.622009569378, Blast_Score=84, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6325086, Length=388, Percent_Identity=36.8556701030928, Blast_Score=206, Evalue=5e-54,
Organism=Saccharomyces cerevisiae, GI6321973, Length=388, Percent_Identity=36.0824742268041, Blast_Score=200, Evalue=2e-52,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001155 [H]

Pfam domain/function: PF00724 Oxidored_FMN [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 40789; Mature: 40658

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLFNEFKLGNTPLSNRVVMAPMTRSRAPEDIAAEQIALHYTQRATAGLIVSEGTPISRE
CCCCCCCCCCCCCCCCCEEECCCHHCCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCCC
GQGYLFNPGIYTPEQIQGWKLVTDSVHSVGGRMFAQLWHVGRVSHTSIQIDGKAPVSATT
CCCEEECCCCCCHHHCCCCEEEHHHHHHHHHHHHHHHHHHCCEECCEEEECCCCCCCHHH
RQAQGAVAFAYGEDGEPGFVPTSAPRPLTTEEVARVVEDFAQAAQNAIDAGFDGVEIHGA
HHCCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEECC
NGYLLEQFLNPLVNDRGDQYGASNLQDRLRFVFEVVDAACARIGADRVGIRISPYGQLFD
CCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCHHHCC
MPLYPEIDETYSALCAGMRERGIAYVHVMDQTHFFMAGESSAAQEQALRKLLKHCKAELG
CCCCCCHHHHHHHHHHHHHHCCEEEEEEECCCEEEEECCCHHHHHHHHHHHHHHHHHHHC
NTALILAGDMTRERADALLEENLIDLAAFGQPFIGNPDLVARLKNGWPLTTPDRDTYYGG
CEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHEECCCCCCCCCCCCCCCCC
DAHGYIDYAPYRA
CCCCEECCCCCCC
>Mature Secondary Structure 
TLFNEFKLGNTPLSNRVVMAPMTRSRAPEDIAAEQIALHYTQRATAGLIVSEGTPISRE
CCCCCCCCCCCCCCCCEEECCCHHCCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCCC
GQGYLFNPGIYTPEQIQGWKLVTDSVHSVGGRMFAQLWHVGRVSHTSIQIDGKAPVSATT
CCCEEECCCCCCHHHCCCCEEEHHHHHHHHHHHHHHHHHHCCEECCEEEECCCCCCCHHH
RQAQGAVAFAYGEDGEPGFVPTSAPRPLTTEEVARVVEDFAQAAQNAIDAGFDGVEIHGA
HHCCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEECC
NGYLLEQFLNPLVNDRGDQYGASNLQDRLRFVFEVVDAACARIGADRVGIRISPYGQLFD
CCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCHHHCC
MPLYPEIDETYSALCAGMRERGIAYVHVMDQTHFFMAGESSAAQEQALRKLLKHCKAELG
CCCCCCHHHHHHHHHHHHHHCCEEEEEEECCCEEEEECCCHHHHHHHHHHHHHHHHHHHC
NTALILAGDMTRERADALLEENLIDLAAFGQPFIGNPDLVARLKNGWPLTTPDRDTYYGG
CEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHEECCCCCCCCCCCCCCCCC
DAHGYIDYAPYRA
CCCCEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: FMN. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH; N-ethylmaleimide [C]

Specific reaction: NADPH + N-ethylmaleimide = NADP + Cytotoxic compound [C]

General reaction: Reduction [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9013822; 9278503 [H]