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The map label for this gene is pilF [H]

Identifier: 66044761

GI number: 66044761

Start: 1700093

End: 1701724

Strand: Reverse

Name: pilF [H]

Synonym: Psyr_1514

Alternate gene names: 66044761

Gene position: 1701724-1700093 (Counterclockwise)

Preceding gene: 66044762

Following gene: 66044760

Centisome position: 27.93

GC content: 56.0

Gene sequence:

>1632_bases
ATGAGCGGCGCGGTACGAATTGAGCCAGACATTGAGGCTGATCCGTTGGCACGCCAGGTCGCCCGGCCGACCGCAGTCGT
GCAACCGATCACCATTGCCATGCAATCTGACGTGGTCGGAACCGTCCTGACTGCGGAGGGAAATCGGTGGGGGGACATCA
CCAGCGAGTTGCGTCAGCTCGTTGCCTTGTTAGCACCTGCCCCAGAGGCTGAAGAAGCAGAATTGCTCGTGTCGTCTCAG
CACATGCACGACCCTTATGTGATGGCATTTATGGACCGCCTGGACCGAGCCGGCGTGCCGTTTCAGACCATCAGCAGCAC
GCTCAGCGACATCAAGTCTCTGTATCAGAATGCGGGTAAAGCTTTCGCCGGATCTATAGACAGTACCCAGCGTCAACAAG
AGGTGATTCGTATCATCGGCGCGGCCCATGCACGACGTGCTTCCGACGTTCACTTCATCGTTGGCCACGAAATCACCTAC
ATCAAGTTTCGTGAGGACGGTCTCTTGCGGGAGTACGGGCAGATCCAGAGCGCCGTAGGAAACGAGCTCTGTTCTTCACT
TTTCAACTCAATGTGTGACGTCAAATCTGAGGGCTACTATCAACCGGAACTGAAACAAGATGCGCGCATGGCTCGCACCT
TCCTCGATCAATTGGGACTGTCAGGGGCCCGTGTAGCAACCCGCCCTCTCGTCGAAGGGCCTTTAATGGTGCTGCGACTG
ATTTATGACGATCAGGAGAAACAGTCCGTCGACGATCTGGGGTTTCTGCCAGAACAGAATTTGCTGTTCAAGCGGCTGAG
AAGTCTTCCTTACGGCGTGATCATATTGACCGGTCCGACTGGATCAGGGAAATCGAAGTCGCTCCAGGTGCAGATCAACC
TTCTGGCTGAAGAGTGCAAAGGCACAAAACATATCCTGACCGTTGAGGACCCGCCCGAATATCCGATGCGTGCAAATCAG
TCCCCTCTTGGCAGCAATGAGTCATGGGGAGAATCCATTACAAACTCCGTGCGACTCGACCCCGACATACTGATGTATGG
GGAGATTCGAGACCTTGAATCAGCTCAGGGGGCACTGCGAGGGGGGATGACCGGCCACCTGGTTTTATCGACTCTGCACA
CCAACAACGCTGTAGCAGCAATTCCTCGCCTGATCGACATGGGGGCTGATACAAGCCTGGTCACCGACCCAACACTCATT
GTCGGCCTTATCAACCAGTCACTGCTGCCAACGCTGTGCAAGAAGTGTCGCGTGCCTGCGCGCAGCCAGATCAGTACCTT
GGATCCGCAGCTGGTCGAGCGGCTCGAAAAGATGACCGTGCTGGATCAGGTCTATCTGGCTGGGCCGGGTTGCTCTCTAT
GCCGTGACGGGGTGAACGGCAGGGCCGCTGTCGCCGAGATTCTTCTCCCGACCCACCGGTTCATGGAGGAGATCCGAAAA
AATGGCCCAAGCGCTGCGCGCCAGTATTGGGTCAAGCACATGGGTGGTATCACGAAGGTTGCCCACACGCTCATCAAGAT
AAATACAGGACTGATTGACCCGCGCATGGCAGAGGCCGTAGTCGGTCCATTGGATTTTGACAGCTACATGCTGGATGCCG
AAGCGCCTGAGGCAGAAGTCCATGCTCAGTAA

Upstream 100 bases:

>100_bases
TTGACGCGCCCCAAATGTCACTGCAGACACTCAAGCCTGTAGCTCCGGGGCAGCCCTCGGCTATGAGTCCGCTGTCCAGC
ATGTCGCAGGAGTTTCAGCG

Downstream 100 bases:

>100_bases
GGTGATGGATGCCTGGTCTGGGCTTGTAGATGGCTTTTACAGGAACCAGTTTGGAGGCAATGAGCGGATCCGGCTTTACG
AGAGCATGACCGCTCTGCTC

Product: type II secretion system protein E

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 543; Mature: 542

Protein sequence:

>543_residues
MSGAVRIEPDIEADPLARQVARPTAVVQPITIAMQSDVVGTVLTAEGNRWGDITSELRQLVALLAPAPEAEEAELLVSSQ
HMHDPYVMAFMDRLDRAGVPFQTISSTLSDIKSLYQNAGKAFAGSIDSTQRQQEVIRIIGAAHARRASDVHFIVGHEITY
IKFREDGLLREYGQIQSAVGNELCSSLFNSMCDVKSEGYYQPELKQDARMARTFLDQLGLSGARVATRPLVEGPLMVLRL
IYDDQEKQSVDDLGFLPEQNLLFKRLRSLPYGVIILTGPTGSGKSKSLQVQINLLAEECKGTKHILTVEDPPEYPMRANQ
SPLGSNESWGESITNSVRLDPDILMYGEIRDLESAQGALRGGMTGHLVLSTLHTNNAVAAIPRLIDMGADTSLVTDPTLI
VGLINQSLLPTLCKKCRVPARSQISTLDPQLVERLEKMTVLDQVYLAGPGCSLCRDGVNGRAAVAEILLPTHRFMEEIRK
NGPSAARQYWVKHMGGITKVAHTLIKINTGLIDPRMAEAVVGPLDFDSYMLDAEAPEAEVHAQ

Sequences:

>Translated_543_residues
MSGAVRIEPDIEADPLARQVARPTAVVQPITIAMQSDVVGTVLTAEGNRWGDITSELRQLVALLAPAPEAEEAELLVSSQ
HMHDPYVMAFMDRLDRAGVPFQTISSTLSDIKSLYQNAGKAFAGSIDSTQRQQEVIRIIGAAHARRASDVHFIVGHEITY
IKFREDGLLREYGQIQSAVGNELCSSLFNSMCDVKSEGYYQPELKQDARMARTFLDQLGLSGARVATRPLVEGPLMVLRL
IYDDQEKQSVDDLGFLPEQNLLFKRLRSLPYGVIILTGPTGSGKSKSLQVQINLLAEECKGTKHILTVEDPPEYPMRANQ
SPLGSNESWGESITNSVRLDPDILMYGEIRDLESAQGALRGGMTGHLVLSTLHTNNAVAAIPRLIDMGADTSLVTDPTLI
VGLINQSLLPTLCKKCRVPARSQISTLDPQLVERLEKMTVLDQVYLAGPGCSLCRDGVNGRAAVAEILLPTHRFMEEIRK
NGPSAARQYWVKHMGGITKVAHTLIKINTGLIDPRMAEAVVGPLDFDSYMLDAEAPEAEVHAQ
>Mature_542_residues
SGAVRIEPDIEADPLARQVARPTAVVQPITIAMQSDVVGTVLTAEGNRWGDITSELRQLVALLAPAPEAEEAELLVSSQH
MHDPYVMAFMDRLDRAGVPFQTISSTLSDIKSLYQNAGKAFAGSIDSTQRQQEVIRIIGAAHARRASDVHFIVGHEITYI
KFREDGLLREYGQIQSAVGNELCSSLFNSMCDVKSEGYYQPELKQDARMARTFLDQLGLSGARVATRPLVEGPLMVLRLI
YDDQEKQSVDDLGFLPEQNLLFKRLRSLPYGVIILTGPTGSGKSKSLQVQINLLAEECKGTKHILTVEDPPEYPMRANQS
PLGSNESWGESITNSVRLDPDILMYGEIRDLESAQGALRGGMTGHLVLSTLHTNNAVAAIPRLIDMGADTSLVTDPTLIV
GLINQSLLPTLCKKCRVPARSQISTLDPQLVERLEKMTVLDQVYLAGPGCSLCRDGVNGRAAVAEILLPTHRFMEEIRKN
GPSAARQYWVKHMGGITKVAHTLIKINTGLIDPRMAEAVVGPLDFDSYMLDAEAPEAEVHAQ

Specific function: Involved in the translocation of the type IV pilin [H]

COG id: COG2804

COG function: function code NU; Type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI1789723, Length=373, Percent_Identity=34.0482573726542, Blast_Score=167, Evalue=2e-42,
Organism=Escherichia coli, GI1786296, Length=387, Percent_Identity=29.9741602067183, Blast_Score=128, Evalue=8e-31,
Organism=Escherichia coli, GI87082188, Length=121, Percent_Identity=39.6694214876033, Blast_Score=89, Evalue=7e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013374
- InterPro:   IPR007831
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E; PF05157 GSPII_E_N [H]

EC number: NA

Molecular weight: Translated: 59412; Mature: 59281

Theoretical pI: Translated: 5.26; Mature: 5.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGAVRIEPDIEADPLARQVARPTAVVQPITIAMQSDVVGTVLTAEGNRWGDITSELRQL
CCCCEEECCCCCCCHHHHHHHCCHHHHCHHEEEEECCCEEEEEEECCCCCCHHHHHHHHH
VALLAPAPEAEEAELLVSSQHMHDPYVMAFMDRLDRAGVPFQTISSTLSDIKSLYQNAGK
HHHHCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCC
AFAGSIDSTQRQQEVIRIIGAAHARRASDVHFIVGHEITYIKFREDGLLREYGQIQSAVG
HHCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCEEEEEEECCCCHHHHHHHHHHHHH
NELCSSLFNSMCDVKSEGYYQPELKQDARMARTFLDQLGLSGARVATRPLVEGPLMVLRL
HHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHCCHHHHHHHHHHH
IYDDQEKQSVDDLGFLPEQNLLFKRLRSLPYGVIILTGPTGSGKSKSLQVQINLLAEECK
HHCCHHHHCHHHHCCCCCHHHHHHHHHCCCCEEEEEECCCCCCCCCEEEEEEEEHHHHCC
GTKHILTVEDPPEYPMRANQSPLGSNESWGESITNSVRLDPDILMYGEIRDLESAQGALR
CCEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHCCEECCCCEEEECCHHHHHHHHHHHH
GGMTGHLVLSTLHTNNAVAAIPRLIDMGADTSLVTDPTLIVGLINQSLLPTLCKKCRVPA
CCCCCEEEEEEEECCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCC
RSQISTLDPQLVERLEKMTVLDQVYLAGPGCSLCRDGVNGRAAVAEILLPTHRFMEEIRK
CHHHHCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
NGPSAARQYWVKHMGGITKVAHTLIKINTGLIDPRMAEAVVGPLDFDSYMLDAEAPEAEV
CCCHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCHHHHHHCCCCCCCCEEECCCCCCCCC
HAQ
CCC
>Mature Secondary Structure 
SGAVRIEPDIEADPLARQVARPTAVVQPITIAMQSDVVGTVLTAEGNRWGDITSELRQL
CCCEEECCCCCCCHHHHHHHCCHHHHCHHEEEEECCCEEEEEEECCCCCCHHHHHHHHH
VALLAPAPEAEEAELLVSSQHMHDPYVMAFMDRLDRAGVPFQTISSTLSDIKSLYQNAGK
HHHHCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCC
AFAGSIDSTQRQQEVIRIIGAAHARRASDVHFIVGHEITYIKFREDGLLREYGQIQSAVG
HHCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCEEEEEEECCCCHHHHHHHHHHHHH
NELCSSLFNSMCDVKSEGYYQPELKQDARMARTFLDQLGLSGARVATRPLVEGPLMVLRL
HHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHCCHHHHHHHHHHH
IYDDQEKQSVDDLGFLPEQNLLFKRLRSLPYGVIILTGPTGSGKSKSLQVQINLLAEECK
HHCCHHHHCHHHHCCCCCHHHHHHHHHCCCCEEEEEECCCCCCCCCEEEEEEEEHHHHCC
GTKHILTVEDPPEYPMRANQSPLGSNESWGESITNSVRLDPDILMYGEIRDLESAQGALR
CCEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHCCEECCCCEEEECCHHHHHHHHHHHH
GGMTGHLVLSTLHTNNAVAAIPRLIDMGADTSLVTDPTLIVGLINQSLLPTLCKKCRVPA
CCCCCEEEEEEEECCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCC
RSQISTLDPQLVERLEKMTVLDQVYLAGPGCSLCRDGVNGRAAVAEILLPTHRFMEEIRK
CHHHHCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
NGPSAARQYWVKHMGGITKVAHTLIKINTGLIDPRMAEAVVGPLDFDSYMLDAEAPEAEV
CCCHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCHHHHHHCCCCCCCCEEECCCCCCCCC
HAQ
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8100347 [H]